Line 279: | Line 279: | ||
<h4>Endolysin Construct</h4> | <h4>Endolysin Construct</h4> | ||
<div> | <div> | ||
− | <img src="https://static.igem.org/mediawiki/2017/c/c7/T--NYMU-Taipei--NS_endolysin_snapgene.png" style="width: | + | <img src="https://static.igem.org/mediawiki/2017/c/c7/T--NYMU-Taipei--NS_endolysin_snapgene.png" style="width:40%;float:right"> |
<p> We successfully constructed <font class='mark_backbone'>J23106-B0034-Endolysin-B0010-B0012</font>. The endolysin in this part is from iGEM released part, BBa_K112806, which is endolysin from enterobacteria phage T4. Besides endolysin, in this composite part, we choose BBa_J23106 as a constitutive promoter, BBa_B0034 as ribosome binding site, BBa_B0010 and BBa_B0012 as double terminator, all of which are widely used parts in iGEM.</p> | <p> We successfully constructed <font class='mark_backbone'>J23106-B0034-Endolysin-B0010-B0012</font>. The endolysin in this part is from iGEM released part, BBa_K112806, which is endolysin from enterobacteria phage T4. Besides endolysin, in this composite part, we choose BBa_J23106 as a constitutive promoter, BBa_B0034 as ribosome binding site, BBa_B0010 and BBa_B0012 as double terminator, all of which are widely used parts in iGEM.</p> | ||
</div> | </div> | ||
Line 301: | Line 301: | ||
<h4></h4> | <h4></h4> | ||
<h4>Holin Construct</h4> | <h4>Holin Construct</h4> | ||
− | <img src="https://static.igem.org/mediawiki/2017/6/62/T--NYMU-Taipei--NS_holin_snapgene.png" style="width: | + | <img src="https://static.igem.org/mediawiki/2017/6/62/T--NYMU-Taipei--NS_holin_snapgene.png" style="width:40%;float:right"> |
<p> We successfully constructed <font class='mark_backbone'>R0010-B0034-Holin-B0010-B0012</font>. To control the precise suicide timing, we choose a lactose-induced promoter, BBa_R0010, to regulate this suicide mechanism. Besides holin and inducible promoter, in this composite part, we also choose BBa_B0034 as ribosome binding site, and BBa_B0010 and BBa_B0012 as double terminator.</p> | <p> We successfully constructed <font class='mark_backbone'>R0010-B0034-Holin-B0010-B0012</font>. To control the precise suicide timing, we choose a lactose-induced promoter, BBa_R0010, to regulate this suicide mechanism. Besides holin and inducible promoter, in this composite part, we also choose BBa_B0034 as ribosome binding site, and BBa_B0010 and BBa_B0012 as double terminator.</p> | ||
<div class="clear"></div> | <div class="clear"></div> | ||
− | <div | + | <div class="half_column"> |
− | <img src="https://static.igem.org/mediawiki/2017/9/9a/T--NYMU-Taipei--NS_holin_PCR.png | + | <img src="https://static.igem.org/mediawiki/2017/9/9a/T--NYMU-Taipei--NS_holin_PCR.png" > |
<p>This figure is electrophoresis result of holin backbone PCR product. | <p>This figure is electrophoresis result of holin backbone PCR product. | ||
The marker is 1kb. The length is 705bp as expected. | The marker is 1kb. The length is 705bp as expected. | ||
</p> | </p> | ||
− | + | </div> | |
− | + | <div class="half_column"> | |
+ | <img src="https://static.igem.org/mediawiki/2017/b/be/T--NYMU-Taipei--NS_holin_BB.png"> | ||
<p>This figure is electrophoresis result of holin PCR product. | <p>This figure is electrophoresis result of holin PCR product. | ||
The marker is 100bp. The length is 657bp as expected. | The marker is 100bp. The length is 657bp as expected. | ||
</p> | </p> | ||
</div> | </div> | ||
+ | <div class="clear"></div> | ||
<h4></h4> | <h4></h4> |
Revision as of 01:42, 29 October 2017