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| | function deletebaton3() { | | function deletebaton3() { |
| | document.getElementById("batonbleu3").style.display = "none"; | | document.getElementById("batonbleu3").style.display = "none"; |
| − | }
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| − |
| |
| − | function apparitionbaton3a() {
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| − | document.getElementById("batonbleu3a").style.display = "block";
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| − | }
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| − |
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| − | function deletebaton3a() {
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| − | document.getElementById("batonbleu3a").style.display = "none";
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| − | }
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| − |
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| − | function apparitionbaton3b() {
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| − | document.getElementById("batonbleu3b").style.display = "block";
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| − | }
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| − |
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| − | function deletebaton3b() {
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| − | document.getElementById("batonbleu3b").style.display = "none";
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| | } | | } |
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| | function deletebaton13() { | | function deletebaton13() { |
| | document.getElementById("batonbleu13").style.display = "none"; | | document.getElementById("batonbleu13").style.display = "none"; |
| − | }
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| − |
| |
| − | function apparitionbaton13b() {
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| − | document.getElementById("batonbleu13b").style.display = "block";
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| − | }
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| − |
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| − | function deletebaton13b() {
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| − | document.getElementById("batonbleu13b").style.display = "none";
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| | } | | } |
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| | function deletebaton15() { | | function deletebaton15() { |
| | document.getElementById("batonbleu15").style.display = "none"; | | document.getElementById("batonbleu15").style.display = "none"; |
| | + | } |
| | + | |
| | + | function apparitionbaton16() { |
| | + | document.getElementById("batonbleu16").style.display = "block"; |
| | + | } |
| | + | |
| | + | function deletebaton16() { |
| | + | document.getElementById("batonbleu16").style.display = "none"; |
| | } | | } |
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| | | | |
| | body{ | | body{ |
| | + | margin:30px; |
| | + | padding:30px; |
| | background-color:white; | | background-color:white; |
| − | width:100%; | + | height:100%; |
| | } | | } |
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| | list-style:none; | | list-style:none; |
| | } | | } |
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| |
| | | | |
| | .left{ | | .left{ |
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| | display:block; | | display:block; |
| | position:relative; | | position:relative; |
| − | top:-50px; | + | top:-10px; |
| − | left:10px; | + | left:0px; |
| | border-bottom:4px dotted black; | | border-bottom:4px dotted black; |
| − | width: 20%; | + | width: 40px; |
| | } | | } |
| | | | |
| − | #caseadroite {
| + | .underline1{ |
| | display:block; | | display:block; |
| − | position: fixed; | + | position:relative; |
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| − | left:35vw; | + | left:0px; |
| − | width:53vw; | + | border-bottom:4px dotted black; |
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| − | font-family: Helvetica, sans-serif;
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| − | content:url(https://static.igem.org/mediawiki/2017/d/d0/T--Pasteur_Paris--Carnet.png); | + | |
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| | | | |
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| − | position: fixed; | + | display:block; |
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| | | | |
| − | .caseadroitetext2 { | + | .underline3{ |
| − | position: fixed; | + | display:block; |
| − | top:230px; | + | position:relative; |
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| − | height:85%; | + | border-bottom:4px dotted black; |
| − | font-family: Helvetica, sans-serif; | + | width: 508px; |
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| − | display:none;
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| − | text-align: justify;
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| | } | | } |
| | | | |
| − | .caseadroitetext3 { | + | .underline4{ |
| − | position: relative; | + | display:block; |
| − | left:33vw; | + | position:relative; |
| − | width:33vw; | + | top:-10px; |
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| − | font-family: Helvetica, sans-serif; | + | border-bottom:4px dotted black; |
| − | font-size: 9pt;
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| − | cursor: default;
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| − | text-align: justify;
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| − | line-height: 18px;
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| | | | |
| − | .caseadroitetext4 { | + | .underline5{ |
| − | position: relative; | + | display:block; |
| − | top:-87vh; | + | position:relative; |
| − | left:63vw; | + | top:-10px; |
| − | width:33vw; | + | left:0px; |
| − | height:85vh; | + | border-bottom:4px dotted black; |
| − | font-family: Helvetica, sans-serif;
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| − | font-size: 9pt;
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| − | text-align: justify;
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| − | line-height: 18px;
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| | | | |
| − | .calendar { | + | .underline6{ |
| − | position: relative; | + | display:block; |
| − | left:300px; | + | position:relative; |
| − | width:65%; | + | top:-10px; |
| − | background: white; | + | left:0px; |
| − | cursor: default; | + | border-bottom:4px dotted black; |
| − | | + | width: 582px; |
| | } | | } |
| − |
| |
| | | | |
| | #top_title{ | | #top_title{ |
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| | | | |
| | .subtitle{ | | .subtitle{ |
| − | margin-left:300px; | + | margin-left:12.25px; |
| − | margin-top:112.5px; | + | margin-top:55px; |
| | cursor: default; | | cursor: default; |
| − | font-size: 26.75pt; | + | font-size: 18pt; |
| | } | | } |
| | | | |
| | | | |
| | .maintext{ | | .maintext{ |
| − | font-size: 30pt; | + | font-size: 9pt; |
| − | margin-left:300px; | + | font-family:Helvetica; |
| − | margin-right:300px;
| + | margin-top:10px; |
| − | margin-top:112.5px; | + | |
| | text-align: justify; | | text-align: justify; |
| | cursor: default; | | cursor: default; |
| − | font-family:Helvetica; | + | width:75%; |
| | } | | } |
| | | | |
| | .maintextsuite{ | | .maintextsuite{ |
| − | font-size: 30pt; | + | font-size: 9pt; |
| − | margin-left:300px;
| + | |
| − | margin-right:300px;
| + | |
| | margin-top:10px; | | margin-top:10px; |
| | text-align: justify; | | text-align: justify; |
| | cursor: default; | | cursor: default; |
| | font-family:Helvetica; | | font-family:Helvetica; |
| | + | width:75%; |
| | } | | } |
| | | | |
| − | .maintextsuite2{ | + | .maintextsuiteimage{ |
| − | font-size: 30pt; | + | font-size: 9pt; |
| − | margin-left:500px;
| + | |
| − | margin-right:300px;
| + | |
| | margin-top:10px; | | margin-top:10px; |
| | text-align: justify; | | text-align: justify; |
| | cursor: default; | | cursor: default; |
| | font-family:Helvetica; | | font-family:Helvetica; |
| | + | width:40%; |
| | + | } |
| | + | |
| | + | .text { |
| | + | position:relative; |
| | + | margin-left: 287.75px; |
| | + | line-height:12pt; |
| | } | | } |
| | | | |
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| | } | | } |
| | | | |
| − | .Jours:hover .caseadroitetext1{ | + | .Chili{ |
| − | display:block;
| + | z-index:-1; |
| − | z-index:100;
| + | margin-left: 287.75px; |
| − | cursor: default;
| + | top:170px; |
| − | color:black;
| + | width:5vw; |
| − | }
| + | height: 150vh; |
| − | .Jours:hover .caseadroitetext2{ | + | |
| − | display:block;
| + | |
| − | z-index:100;
| + | |
| − | cursor: default;
| + | |
| − | color:black;
| + | |
| | } | | } |
| | | | |
| − | .Jours:hover{
| + | table{ |
| − | color: #3298CB;
| + | border-collapse: collapse; |
| − | font-weight:bold; | + | } |
| − | }
| + | |
| − | | + | |
| − | .Jours{
| + | |
| − | text-align:center; | + | |
| − | } | + | |
| | | | |
| | /*////////////////////////////////////////////*/ | | /*////////////////////////////////////////////*/ |
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| | top: 26%; | | top: 26%; |
| | } | | } |
| | + | |
| | | | |
| | .outer5 { | | .outer5 { |
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| | position: fixed; | | position: fixed; |
| | left: 40px; | | left: 40px; |
| − | top: 42%; | + | top: 50%; |
| | + | } |
| | + | |
| | + | .outer16 { |
| | + | position: fixed; |
| | + | left: 40px; |
| | + | top: 36%; |
| | } | | } |
| | | | |
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| | } | | } |
| | /*////////////////////////////////////////////*/ | | /*////////////////////////////////////////////*/ |
| | + | |
| | | | |
| | .rotate { | | .rotate { |
| | -webkit-transform: rotate(180deg); | | -webkit-transform: rotate(180deg); |
| − | }
| |
| − |
| |
| − | .list a{
| |
| − | text-decoration: none;
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| − | color:black;
| |
| | } | | } |
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| | top:5px; | | top:5px; |
| | left:10%; | | left:10%; |
| | + | display:none; |
| | + | height:3px; |
| | + | width:22.5px; |
| | + | background: #3298CB; |
| | + | content:"" |
| | + | } |
| | + | |
| | + | #batonbleu16{ |
| | + | position:relative; |
| | + | top:5px; |
| | + | left:35%; |
| | display:none; | | display:none; |
| | height:3px; | | height:3px; |
| Line 1,025: |
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| | background: #3298CB; | | background: #3298CB; |
| | content:"" | | content:"" |
| | + | } |
| | + | |
| | + | li{margin:5px} |
| | + | |
| | + | a:visited { |
| | + | color: black; |
| | + | text-decoration:none; |
| | + | } |
| | + | |
| | + | |
| | + | |
| | + | /*////////////////////////////////*/ |
| | + | |
| | + | |
| | + | #title{ |
| | + | font-size: 60pt; |
| | + | border-bottom:4px dotted black; |
| | + | width: 450px; |
| | + | z-index:-1; |
| | + | margin-left: 287.75px; |
| | + | margin-top: 90px; |
| | + | line-height:59pt; |
| | + | } |
| | + | |
| | + | #subtitles{ |
| | + | position:relative; |
| | + | margin-left: 300px; |
| | + | margin-top:-5vw; |
| | + | font-size: 18pt; |
| | + | font-weight: normal; |
| | + | /*border-bottom:4px dotted black;*/ |
| | + | z-index:-1; |
| | + | width: 250px; |
| | + | border-bottom:4px dotted black; |
| | } | | } |
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| | | | |
| | | | |
| − | <body onload="$('#caseadroite').followTo(390); ; periodiqueapparition()""> | + | <body> |
| − | <div style="position:fixed; width:100%; background: white; z-index:10; opacity: 100%"></div>
| + | |
| | <a class="left" href="https://2017.igem.org/Team:Pasteur_Paris"><img src="https://static.igem.org/mediawiki/2017/d/d8/T--Pasteur_Paris--aether.png"; width="220px"; height="70px"></img></a> | | <a class="left" href="https://2017.igem.org/Team:Pasteur_Paris"><img src="https://static.igem.org/mediawiki/2017/d/d8/T--Pasteur_Paris--aether.png"; width="220px"; height="70px"></img></a> |
| | <a class="right" href="https://2017.igem.org/Team:Pasteur_Paris"><img src="https://static.igem.org/mediawiki/2017/1/10/T--Pasteur_Paris--iGEM.png"; width="90px"; height="70px"></img></a> | | <a class="right" href="https://2017.igem.org/Team:Pasteur_Paris"><img src="https://static.igem.org/mediawiki/2017/1/10/T--Pasteur_Paris--iGEM.png"; width="90px"; height="70px"></img></a> |
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| | <div class="inner"; onmouseover="apparitionbaton9()"; onmouseout="deletebaton9()"> | | <div class="inner"; onmouseover="apparitionbaton9()"; onmouseout="deletebaton9()"> |
| | <div class="list rotate"> | | <div class="list rotate"> |
| − | <a href="https://2017.igem.org/Team:Pasteur_Paris/Interlab">Interlab</a> | + | <a href="https://2017.igem.org/Team:Pasteur_Paris/InterLab">InterLab</a> |
| | <div id="batonbleu9"> | | <div id="batonbleu9"> |
| | </div> | | </div> |
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| | <a href="https://2017.igem.org/Team:Pasteur_Paris/Parts">Parts</a> | | <a href="https://2017.igem.org/Team:Pasteur_Paris/Parts">Parts</a> |
| | <div id="batonbleu15"> | | <div id="batonbleu15"> |
| | + | </div> |
| | + | </div> |
| | + | </div> |
| | + | </div> |
| | + | <div class="outer16"> |
| | + | <div class="inner"; onmouseover="apparitionbaton16()"; onmouseout="deletebaton16()"> |
| | + | <div class="list rotate"> |
| | + | <a href="https://2017.igem.org/Team:Pasteur_Paris/GiantJamboree">GiantJamboree</a> |
| | + | <div id="batonbleu16"> |
| | </div> | | </div> |
| | </div> | | </div> |
| Line 1,245: |
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| | </div> | | </div> |
| | </div> | | </div> |
| | + | |
| | <ul class="com"> | | <ul class="com"> |
| | <li style="text-align:center"><a href="https://www.facebook.com/igem.pasteur/" target="_blank"><i id="logo" class="fa fa-facebook"></i></a></li> | | <li style="text-align:center"><a href="https://www.facebook.com/igem.pasteur/" target="_blank"><i id="logo" class="fa fa-facebook"></i></a></li> |
| Line 1,252: |
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| | </ul> | | </ul> |
| | | | |
| − | <div class="subtitle"> | + | <div> |
| − | <p style="font-size:50pt; font-family: CrimsonTextRoman, serif;">Labwork</p> | + | |
| − | <div class="underline"></div><br><br><br><br> | + | <div style="height:120vh"> |
| | + | <img src="https://static.igem.org/mediawiki/2017/a/ab/T--Pasteur_Paris--MainDesign.jpeg"; style="position:absolute; left:390px; height: auto;width: 55vw; z-index:-2;top:-70px"/> |
| | + | <p id="title" style="font-size:50pt; font-family: MinionPro, serif; text-align:left">Welcome to the <br> team iGEM Pasteur 2017's webpage !</p><br></p> |
| | + | <div class="Chili"></div> |
| | </div> | | </div> |
| | | | |
| | + | <div class="text" style="margin-top:20vh"> |
| | + | <p class="maintext">For the third year in a row, the Institut Pasteur hosts a multidisciplinary student team composed of Biology, Chemistry, Engineering but also Industrial Design and Law students. After the success of the 2015 and 2016 teams, which were rewarded by bronze and golden medals respectively, we have all the assets needed to maintain momentum.</p> |
| | | | |
| − |
| + | <p class="maintextsuite">Our team's research focuses on the issue of air pollution. This worldwide disaster is responsible for the death of between 7.3 to 9.0 million people per year, according to WHO's 2012 and Lancet 2017 reports. It concerns both indoor and outdoor air, especially in urban areas. Pollution in the domestic setting is a particularly big issue, even in rural areas. Indeed, we spend 80 % or more of our time indoors. Air contamination is also a threat to many ecosystems and infrastructures. Even though there is a rising awareness about the dangers of this issue, too few solutions exist. Our will is to design an innovative, biodegradable and affordable device to fight air pollution. |
| − | <div class="calendar" style="border:0; padding: 2px;"> | + | |
| − | <table>
| + | |
| − | <tr>
| + | |
| − | <td colspan="7" style="font-family: CrimsonTextRoman, serif; font-weight:bold">July
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td style="text-align:center">M</td>
| + | |
| − | <td style="text-align:center">T</td>
| + | |
| − | <td style="text-align:center">W</td>
| + | |
| − | <td style="text-align:center">T</td>
| + | |
| − | <td style="text-align:center">F</td>
| + | |
| − | <td style="text-align:center">S</td>
| + | |
| − | <td style="text-align:center">S</td>
| + | |
| − | </tr>
| + | |
| − | <tr id="cal">
| + | |
| − | <div class="Jours">
| + | |
| | | | |
| − | <td></td>
| + | <br><br><br><br><br><br><br><br><br><br><br><br></p> |
| − | <td></td>
| + | |
| − | <td></td>
| + | |
| − | <td></td>
| + | |
| − | <td></td>
| + | |
| − | | + | |
| − | | + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 1
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 2
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | | + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | | + | |
| − | </tr><tr>
| + | |
| − | | + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 3
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 4
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 5
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − | Operators: Deshmukh, Ersin, Gabriel, Alexis
| + | |
| − | <br>
| + | |
| − | Transformation of bacteria DH5α with pet43.1a.C162, for the positive control we will use the plasmid pUC19 + a negative control
| + | |
| − | according to the transformation protocol except for volumes.
| + | |
| − | <br>
| + | |
| − | <br><br>
| + | |
| − | Observations 16h later:
| + | |
| − | <li>• The culture for the positive test revealed mean bacterial cultures (more than 50) this test confirms our DH5α cells are competent</li>
| + | |
| − | <li>• The culture for the negative test did not reveal any bacterial culture, which proved that the cells without any plasmids couldn’t survive in an antibiotic based media</li>
| + | |
| − | <li>• The cell cultures containing pET43.1a.C162 do not present any colonies; there must be a problem with the pET43.1a.C162 solution, because the DH5α cells are truly competent</li>
| + | |
| − | <li>• By spectroscopy UV-visible using the equipment UV5 Nano Mettler Toledo, at 260 nm with a 1µL sample, we measured a plasmid concentration of 0, the solution used did not contain plasmids, which explains the death of our cultures</li><br><br>For the detailed protocols followed, refer to the PDF documents below
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 6
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − | Operators: Deshmukh, Ersin, Gabriel, Alexis
| + | |
| − | <br>
| + | |
| − | Transformation of plasmids pET43.1a.C162 in DH5α (2nd test) according to the transformation protocol except for volumes
| + | |
| − | <br><br><br><br><br><br><br><br><br><br>For the detailed protocols followed, refer to the PDF documents below
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − | Operators: Deshmukh, Ersin, Gabriel, Alexis
| + | |
| − | <br>
| + | |
| − | Petri Dish preparation according to the preparation protocol except for volumes.
| + | |
| − | 33 Dish total
| + | |
| − | <br><br>
| + | |
| − | Then the Petri Dish are stored at 4°C or placed at the incubator at 37°C for immediate use.<br><br><br><br><br><br><br><br>For the detailed protocols followed, refer to the PDF documents below
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 7
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − | Culture in liquid media of DH5α containing pET43.1a.C162 according to the protocol of liquid culture except for the volumes.
| + | |
| − | <br><br>
| + | |
| − | <li> The pellet is stored at -20°C named: DH5α pET43.1a.C162. </li> <br><br><br><br><br><br><br><br>For the detailed protocols followed, refer to the PDF documents below
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 8
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 9
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | | + | |
| − | </tr><tr>
| + | |
| − | | + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 10
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | | + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 11
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − | Operators: Deshmukh, Karima, Pierre-Louis, Juliette, Paul, Nathan, Diane
| + | |
| − | <br>
| + | |
| − | Culture in liquid media of DH5α containing pET43.1a.C162 according to the protocol of liquid culture except for the volumes.
| + | |
| − | <br><br>
| + | |
| − | Note: the Erlenmeyer number 5 was given 2 doses of antibiotics so we have 50µL of antibiotics for 25mL of LB broth media.
| + | |
| − | <br><br><br><br><br><br><br><br><br>For the detailed protocols followed, refer to the PDF documents below
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 12
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − | Operators: Juliette, Karima, Paul
| + | |
| − | <br>
| + | |
| − | Midiprep of pET43.1a.C162 previous liquid culture with the Qiafilter Midi kit from Qiagen.
| + | |
| − | <br><br>
| + | |
| − | Four samples with an average concentration of 60 ng/µL where store at -20°C.
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − | Operators: Ersin, Gabriel, Alexis
| + | |
| − | <br>
| + | |
| − | Digestion of the plasmid pET43.1a.C162 according to the digestion protocol except for the volumes with BamHI and XbaI.
| + | |
| − | <br><br>
| + | |
| − | Launch of an electrophoresis in order to extract the empty plasmid pET43.1a.
| + | |
| − | <br><br>
| + | |
| − | The cutted gel were stored at 4°C<br><br><br><br><br><br><br><br>For the detailed protocols followed, refer to the PDF documents below
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 13
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − | Operators: Ersin, Gabriel, Alexis
| + | |
| − | <br>
| + | |
| − | Gel extraction of the plasmid pET43.1a with the Qiagen gel extraction kit.
| + | |
| − | <br><br>
| + | |
| − | The sample is stored at -20°C, with an average concentration of 7,418 ng/µL.<br><br><br><br><br><br><br><br>For the detailed protocols followed, refer to the PDF documents below
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − | Operators: Gabriel, Paul
| + | |
| − | <br>
| + | |
| − | Electrophoresis of pET43.1a.C162 midiprep from the 12/10/2017.
| + | |
| − | <br><br>
| + | |
| − | Check of the plasmid purity.<br><br><br><br><br><br><br><br>For the detailed protocols followed, refer to the PDF documents below
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 14
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 15
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 16
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | | + | |
| − | </tr><tr>
| + | |
| − | | + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 17
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − | Operators: Paul, Ersin, Diane, Alexis
| + | |
| − | <br>
| + | |
| − | Digestion of plasmids pET43.1a.C162
| + | |
| − | <br><br>
| + | |
| − | Launch of an electrophoresis in order to extract the empty plasmid pET43.1a.
| + | |
| − | <br><br>
| + | |
| − | The cutted gel were stored at 4°C<br><br><br><br><br><br><br><br>For the detailed protocols followed, refer to the PDF documents below
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 18
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − | Operators: Paul, Ersin, Gabriel, Diane
| + | |
| − | <br>
| + | |
| − | Gel extraction of the plasmid pET43.1a with the Qiagen gel extraction kit.
| + | |
| − | <br><br>
| + | |
| − | Four samples with an average concentration of 61 ng/µL where store at -20°C.<br><br><br><br><br><br><br><br>For the detailed protocols followed, refer to the PDF documents below
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 19
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − | Operators: Ersin, Alexis
| + | |
| − | <br>
| + | |
| − | Solubilisation of Eurofins Plasmid E2 gene in TE 0.1X : pEXA128-E2.
| + | |
| − | <br><br><br>
| + | |
| − | Operators: Diane, Ersin, Gabriel, Alexis, Karima
| + | |
| − | <br><br>
| + | |
| − | Protocol for Bacteria DH5α Transformation with pEXA128-E2 <br><br><br><br><br><br><br><br>For the detailed protocols followed, refer to the PDF documents below
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − | Operators: Gabriel, Ersin, Alexis, Diane, Karima
| + | |
| − | <br>
| + | |
| − | Dephosphorylation of plasmid or DNA: pET43.1a (X-B)<br><br><br><br><br><br><br><br>For the detailed protocols followed, refer to the PDF documents below
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 20
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 21
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 22
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 23
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − | Operator: Deshmukh
| + | |
| − | <br>
| + | |
| − | Liquid Culture for miniprep on transformed Bacteria DH5α pEXA128-E2.<br><br><br><br><br><br><br><br>For the detailed protocols followed, refer to the PDF documents below
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | | + | |
| − | </tr><tr>
| + | |
| − | | + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 24
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − | Operators: Alexis, Juliette
| + | |
| − | <br>
| + | |
| − | Petri Dish preparation according to the preparation protocol except for volumes.
| + | |
| − | <br>
| + | |
| − | 20 Dish total
| + | |
| − | <br><br>
| + | |
| − | Then the Petri Dish are stored at 4°C or placed at the incubator at 37°C for immediate use.
| + | |
| − | <br><br>
| + | |
| − | Operator: Gabriel
| + | |
| − | <br>
| + | |
| − | Solubilisation of the lyophilized Eurofins plasmid pEXA128 E1_2, for its future transformation
| + | |
| − | <br><br>
| + | |
| − | Operators: Alexis, Juliette, Karima
| + | |
| − | <br>
| + | |
| − | Transformation of DH5α with pSB1C3
| + | |
| − | <br><br>
| + | |
| − | Operators: Diane, Nathan
| + | |
| − | <br>
| + | |
| − | Transformation of DH5α with pEXA128 E1_2. <br><br><br><br><br><br><br><br>For the detailed protocols followed, refer to the PDF documents below
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − | Operators: Ersin, Gaétan
| + | |
| − | <br>
| + | |
| − | Miniprep of previous liquid culture with the QIAprep Spin Miniprep kit from Qiagen.
| + | |
| − | <br>
| + | |
| − | Four samples with an average concentration of 280 ng/µL.
| + | |
| − | <br><br>
| + | |
| − | Operators: Ersin, Gabriel
| + | |
| − | <br>
| + | |
| − | Digestion of the plasmid pEXA128 E2 according to the digestion protocol except for the volumes with BamHI and XbaI to retrieve our gene E2.
| + | |
| − | <br><br>
| + | |
| − | Operator: Gabriel
| + | |
| − | <br>
| + | |
| − | Electrophoresis gel preparation
| + | |
| − | <br>
| + | |
| − | Electrophoresis run<br><br><br><br><br><br><br><br>For the detailed protocols followed, refer to the PDF documents below
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 25
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − | Operators: Juliette Alexis
| + | |
| − | <br><br>
| + | |
| − | Liquid Culture for Miniprep on transformed Bacteria DH5α pEXA128-E1_2
| + | |
| − | <br><br>
| + | |
| − | Operators: Karima, Diane
| + | |
| − | <br><br>
| + | |
| − | Gel Extraction of the gene E2 with QIAquick Gel Extraction Kit:
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − | Operators: Karima, Juliette, Alexis
| + | |
| − | <br><br>
| + | |
| − | Transformation of DH5α by pSB1C3
| + | |
| − | <br><br>
| + | |
| − | Operators: Karima, Diane
| + | |
| − | <br><br>
| + | |
| − | Ligation between dephosphorylated pET43.1a and E2<br><br><br><br><br><br><br><br>For the detailed protocols followed, refer to the PDF documents below
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 26
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − | Operators: Karima, Diane
| + | |
| − | <br><br>
| + | |
| − | Miniprep on transformed Bacteria DH5α pEXA128-E1_2
| + | |
| − | <br><br>
| + | |
| − | • Four samples with an average concentration of 320 ng/µL.
| + | |
| − | <br><br>
| + | |
| − | Plasmid Digestion and Gel extraction of pEXA128-E1_2
| + | |
| − | <br><br>
| + | |
| − | Ligation of plasmid with DNA insert pEXA128-E1_2<br><br><br><br><br><br><br><br>For the detailed protocols followed, refer to the PDF documents below
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − | Operators: Ersin Gabriel
| + | |
| − | <br><br>
| + | |
| − | DNA Digestion pEXA128-E2<br><br><br><br><br><br><br><br>For the detailed protocols followed, refer to the PDF documents below
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 27
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 28
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 29
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 30
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | | + | |
| − | </tr><tr>
| + | |
| − | | + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 31
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − | Operators: Alexis, Ersin
| + | |
| − | <br>
| + | |
| − | Solubilisation of Eurofins Plasmid E3 gene: pEXA258-E3
| + | |
| − | <br><br>
| + | |
| − | Solubilisation of Eurofins Plasmid E1_1 gene: pEXA258-E1_1
| + | |
| − | <br><br><br>
| + | |
| − | Operators: Karima, Diane
| + | |
| − | <br>
| + | |
| − | DNA Digestion of pEX-E2 and pEX-E1_2 with XbaI and BamHI<br><br><br><br><br><br><br><br>For the detailed protocols followed, refer to the PDF documents below
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − | Operators: Alexis, Ersin
| + | |
| − | <br>
| + | |
| − | Transformation of DH5α with pEXA258-E1_1:
| + | |
| − | <br><br>
| + | |
| − | Protocol for Bacteria Transformation of DH5α with pEXA258-E3<br><br><br><br><br><br><br><br>For the detailed protocols followed, refer to the PDF documents below
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | </table>
| + | |
| − | | + | |
| − | <br>
| + | |
| − |
| + | |
| − | <table border="0">
| + | |
| − | <tr>
| + | |
| − | <td colspan="7" style="font-family: CrimsonTextRoman, serif; font-weight:bold">August
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td style="text-align:center">M</td>
| + | |
| − | <td style="text-align:center">T</td>
| + | |
| − | <td style="text-align:center">W</td>
| + | |
| − | <td style="text-align:center">T</td>
| + | |
| − | <td style="text-align:center">F</td>
| + | |
| − | <td style="text-align:center">S</td>
| + | |
| − | <td style="text-align:center">S</td>
| + | |
| − | </tr>
| + | |
| − | <tr id="cal">
| + | |
| − | <div class="Jours">
| + | |
| − | | + | |
| − | <td></td>
| + | |
| − | | + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 1
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − | Operators: Diane, Ersin
| + | |
| − | <br>
| + | |
| − | Digestion of plasmid pET43.1a.C162
| + | |
| − | <br><br>
| + | |
| − | Electrophoresis
| + | |
| − | <br><br>
| + | |
| − | Gel Extraction with QIAquick Gel Extraction Kit<br><br><br><br><br><br><br><br>For the detailed protocols followed, refer to the PDF documents below
| + | |
| − | <br><br>
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − | Operators: Diane, Alexis
| + | |
| − | <br>
| + | |
| − | Liquid Culture for Miniprep on transformed Bacteria DH5α pEXA258-E1_1 and pEXA258-E3 <br><br><br><br><br><br><br><br>For the detailed protocols followed, refer to the PDF documents below
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 2
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − | Operators: Ersin, Diane
| + | |
| − | <br>
| + | |
| − | Miniprep on transformed Bacteria DH5α pEXA258-E1_1 and pEXA258-E3
| + | |
| − | <br><br>
| + | |
| − | Ligation of plasmid pET43.1a with DNA insert E2 col 2 and E1_2 col 2
| + | |
| − | <br><br>
| + | |
| − | Gel Extraction of pET43.1a with QIAquick Gel Extraction Kit
| + | |
| − | <br><br>
| + | |
| − | Digestion of DNA pEXA258-E1_1 and pEXA258-E3 with XbaI and BamHI <br><br><br><br><br><br><br><br>For the detailed protocols followed, refer to the PDF documents below
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − | Operators: Ersin, Diane
| + | |
| − | <br>
| + | |
| − | Transformation of DH5α with pET43.1a-E1_2
| + | |
| − | <br><br>
| + | |
| − | Transformation of DH5α with pET43.1a-E2 or pET43.1a
| + | |
| − | <br><br>
| + | |
| − | Dephosphorylation of plasmid pET43.1a with XbaI and BamHI<br><br><br><br><br><br><br><br>For the detailed protocols followed, refer to the PDF documents below
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 3
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − | Operators: Ersin, Diane
| + | |
| − | <br>
| + | |
| − | Gel Extraction of E1_1 and E3 with QIAquick Gel Extraction Kit
| + | |
| − | <br><br>
| + | |
| − | Ligation of plasmid pET43.1a with DNA inserts E3 col 1 and E1-1 col 3<br><br><br><br><br><br><br><br>For the detailed protocols followed, refer to the PDF documents below
| + | |
| − | <br><br>
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − | Operators: Diane, Alexis
| + | |
| − | <br>
| + | |
| − | Liquid Culture for Miniprep on transformed Bacteria DH5α pET43.1a-E2
| + | |
| − | <br><br>
| + | |
| − | Operators: Ersin, Diane
| + | |
| − | <br>
| + | |
| − | Protocol for Bacteria Transformation of DH5α with pET43.1a-E1-1 and pET43.1a-E3<br><br><br><br><br><br><br><br>For the detailed protocols followed, refer to the PDF documents below
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 4
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − | Operators: Alexis, Paul
| + | |
| − | <br>
| + | |
| − | Miniprep on transformed Bacteria DH5α pET43.1a-E2
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − | Operators: Alexis, Paul
| + | |
| − | <br>
| + | |
| − | Digestion of pET43.1a-E2 with XbaI and BamHI<br><br><br><br><br><br><br><br>For the detailed protocols followed, refer to the PDF documents below
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 5
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 6
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | | + | |
| − | </tr><tr>
| + | |
| − | | + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 7
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − | Operators: Ersin, Diane
| + | |
| − | <br>
| + | |
| − | Digestion of pET43.1a-E1_1 and pET43.1a-E1_2 with XbaI and BamHI
| + | |
| − | <br><br>
| + | |
| − | DNA pET43.1a-E3 Digestion X-B<br><br><br><br><br><br><br><br>For the detailed protocols followed, refer to the PDF documents below
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − | Operators: Ersin, Diane
| + | |
| − | <br>
| + | |
| − | Transformation BL21DE3 pET43.1a-E1_1
| + | |
| − | <br><br>
| + | |
| − | Transformation BL21DE3 pET43.1a-E1_2
| + | |
| − | <br><br>
| + | |
| − | Transformation BL21DE3 pET43.1a-E2
| + | |
| − | <br><br>
| + | |
| − | Transformation BL21DE3 pET43.1a-E3<br><br><br><br><br><br><br><br>For the detailed protocols followed, refer to the PDF documents below
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 8
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − | Operators: Gabriel, Diane
| + | |
| − | <br>
| + | |
| − | Preparation of solutions of plasmids pET43.1a-E1_1, pET43.1a-E1_2, pET43.1a-E2 and pET43.1a-E3, for sequencing by Eurofins
| + | |
| − | <br><br>
| + | |
| − | Operators: Diane, Azéline
| + | |
| − | <br>
| + | |
| − | Liquid Culture of transformed Bacteria BL21DE3 pET43.1a-E1-2 and pET43.1a-E2<br><br><br><br><br><br><br><br>For the detailed protocols followed, refer to the PDF documents below
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − | Operators: Ersin, Diane
| + | |
| − | <br>
| + | |
| − | Transformation BL21DE3 pET43.1a-E1_1
| + | |
| − | <br><br>
| + | |
| − | Transformation BL21DE3 pET43.1a-E1_2
| + | |
| − | <br><br>
| + | |
| − | Transformation BL21DE3 pET43.1a-E2
| + | |
| − | <br><br>
| + | |
| − | Transformation BL21DE3 pET43.1a-E3 <br><br><br><br><br><br><br><br>For the detailed protocols followed, refer to the PDF documents below
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 9
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − | Operators: Gaétan, Gabriel
| + | |
| − | <br>
| + | |
| − | Expression culture and kinetic BL21 pET43.1a-E1_2 and pET43.1a-E2<br><br><br><br><br><br><br><br>For the detailed protocols followed, refer to the PDF documents below
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − | Operators: Diane, Azéline
| + | |
| − | <br>
| + | |
| − | Transformation BL21DE3 pET43.1a-E1_1, pET43.1a-E1_2, pET43.1a-E2, pET43.1a-E3<br><br><br><br><br><br><br><br>For the detailed protocols followed, refer to the PDF documents below
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 10
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − | Operators: Gaétan, Gabriel
| + | |
| − | <br>
| + | |
| − | Liquid culture of BL21DE3 pET43.1a-E1-2 and pET43.1a-E2<br><br><br><br><br><br><br><br>For the detailed protocols followed, refer to the PDF documents below
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − | Operators: Gabriel, Karima
| + | |
| − | <br>
| + | |
| − | Bacteria lysis and SDS Page gel migration of Bl21DE3 pET43.1a-E2 and pET43.1a-E1_2 <br><br><br><br><br><br><br><br>For the detailed protocols followed, refer to the PDF documents below
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 11
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − | Operators: Gaétan, Diane
| + | |
| − | <br>
| + | |
| − | Liquid culture of BL21De3 pET43.1a-E1-1 and pET43.1a-E3<br><br><br><br><br><br><br><br>For the detailed protocols followed, refer to the PDF documents below
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 12
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 13
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | | + | |
| − | </tr><tr>
| + | |
| − | | + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 14
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 15
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 16
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 17
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 18
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − | Operators: Gabriel, Diane, Alexis
| + | |
| − | <br>
| + | |
| − | Bacteria lysis and SDS Page gel migration<br><br><br><br><br><br><br><br>For the detailed protocols followed, refer to the PDF documents below
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 19
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 20
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | | + | |
| − | </tr><tr>
| + | |
| − | | + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 21
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − | Operators: Diane, Nathan
| + | |
| − | <br>
| + | |
| − | InterLab Transformation<br><br><br><br><br><br><br><br>For the detailed protocols followed, refer to the PDF documents below
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 22
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − | Operators : Ersin, Diane
| + | |
| − | <br>
| + | |
| − | Transformation BL21DE3 pET-E3
| + | |
| − | <br><br>
| + | |
| − | Transformation DH5α and BL21DE3 pET-E1_1<br><br><br><br><br><br><br><br>For the detailed protocols followed, refer to the PDF documents below
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 23
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − | Operators: Alexis
| + | |
| − | <br>
| + | |
| − | Solubilisation of Eurofins Plasmid E4 gene: pEXA258-E4
| + | |
| − | <br>
| + | |
| − | Transformation DH5α pEX-E4 <br><br><br><br><br><br><br><br>For the detailed protocols followed, refer to the PDF documents below
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
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| − | <td>
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| − | 24
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| − | </div>
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| − | </td>
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| − | <td>
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| − | 27
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| − | <div class="caseadroitetext1">
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| − | </div>
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| − | </tr><tr>
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| + | |
| − | <div class=Jours>
| + | |
| − | 28
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − | Operators : Nathan, Diane
| + | |
| − | <br>
| + | |
| − | Transformation of DH5α with pSB1C3 of E1_1, E1_2, E2, E3 and E4<br><br><br><br><br><br><br><br>For the detailed protocols followed, refer to the PDF documents below
| + | |
| − | </div>
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| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
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| − |
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| − | </div>
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| − | </div>
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| − | </td>
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| − | <div class=Jours>
| + | |
| − | 29
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − | Operators : Paul, Diane
| + | |
| − | <br>
| + | |
| − | Transformation of DH5α with pSB1C3 of E1_1, E1_2, E2, E3 and E4<br><br><br><br><br><br><br><br>For the detailed protocols followed, refer to the PDF documents below
| + | |
| − | </div>
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| − | <div class="caseadroitetext2">
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| − | <br><br><br><br>
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| − | </div>
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| − | 30
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| − |
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| − | </div>
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| − | </div>
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| − | <td colspan="7" style="font-family: CrimsonTextRoman, serif; font-weight:bold">September
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| − | <td style="text-align:center">M</td>
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| − | <td style="text-align:center">W</td>
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| − | <td style="text-align:center">T</td>
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| − | <td style="text-align:center">F</td>
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| − | <td style="text-align:center">S</td>
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| − | <td style="text-align:center">S</td>
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| − | </tr>
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| − | 3
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| − | 4
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| − | <div class="caseadroitetext1">
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| − | <br><br><br><br>
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| − |
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| − | </div>
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| − | <div class="caseadroitetext2">
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| − |
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| − | </div>
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| − | </div>
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| − | </td>
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| − | <td>
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| − | <div class=Jours>
| + | |
| − | 5
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| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − |
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| − | </div>
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| − | <div class="caseadroitetext2">
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| − | <br><br><br><br>
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| − |
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| − | </div>
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| − | </div>
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| − | </td>
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| − | <div class=Jours>
| + | |
| − | 6
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| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − | Operators: Maud, Paul, Diane
| + | |
| − | <br>
| + | |
| − | DNA Digestion and Gel extraction of pET32a<br><br><br><br><br><br><br><br>For the detailed protocols followed, refer to the PDF documents below
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − | Operators: Maud, Diane
| + | |
| − | <br>
| + | |
| − | Dephosphorylation of pET32a<br><br><br><br><br><br><br><br>For the detailed protocols followed, refer to the PDF documents below
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 7
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − | Operators : Maud, Diane
| + | |
| − | <br>
| + | |
| − | Transformation of DH5α with pET32a of E1_1, E1_2, E2, E3 and E4 <br><br><br><br><br><br><br><br>For the detailed protocols followed, refer to the PDF documents below
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − |
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| − | </div>
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| − | </div>
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| − | </td>
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| − | <td>
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| − | <div class=Jours>
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| − | 8
| + | |
| − | <div class="caseadroitetext1">
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| − | <br><br><br><br>
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| − |
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| − | </div>
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| − | <div class="caseadroitetext2">
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| − | </div>
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| − | 9
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| − | <div class="caseadroitetext1">
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| − |
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| − | 10
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| − | 11
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| − | </div>
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| − | </td>
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| − | 12
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| − | </div>
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| − | 13
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| − | 14
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| − | 15
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| − | 16
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| − | 19
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| − | 26
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| − | <div class="caseadroitetext1">
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| − | 27
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| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 28
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − | Operators: Ersin, Gaétan, Deshmukh
| + | |
| − | <br>
| + | |
| − | Miniprep on pEX-S1 transformed Bacteria<br><br><br><br><br><br><br><br>For the detailed protocols followed, refer to the PDF documents below
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − | Operators: Ersin, Gaétan, Deshmukh
| + | |
| − | <br>
| + | |
| − | Transformation of DH5α with pSB1C3-E1_1; E1_2 and E2
| + | |
| − | <br><br>
| + | |
| − | Transformation of DH5α pET32a-E1_1; E1_2 and E2 <br><br><br><br><br><br><br><br>For the detailed protocols followed, refer to the PDF documents below
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 29
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 30
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
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| − | <div class="caseadroitetext2">
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| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | </table>
| + | |
| − | | + | |
| − | <br>
| + | |
| − | | + | |
| − | <table border="0">
| + | |
| − | <tr>
| + | |
| − | <td colspan="7" style="font-family: CrimsonTextRoman, serif; font-weight:bold">October
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td style="text-align:center">M</td>
| + | |
| − | <td style="text-align:center">T</td>
| + | |
| − | <td style="text-align:center">W</td>
| + | |
| − | <td style="text-align:center">T</td>
| + | |
| − | <td style="text-align:center">F</td>
| + | |
| − | <td style="text-align:center">S</td>
| + | |
| − | <td style="text-align:center">S</td>
| + | |
| − | </tr>
| + | |
| − | <tr id="cal">
| + | |
| − | <div class="Jours">
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| − | | + | |
| − | <td></td>
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| − | <td></td>
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| − | <td></td>
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| − | <td></td>
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| − | <td></td>
| + | |
| − | <td></td>
| + | |
| − | | + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 1
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | | + | |
| − | </tr><tr>
| + | |
| − | | + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 2
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 3
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 4
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 5
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − | Operators: Juliette and Ersin
| + | |
| − | <br>
| + | |
| − | Digestion of pET32a with E1_1; E1_2 and E2
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 6
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 7
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − | Operators: Deshmukh and Juliette
| + | |
| − | <br>
| + | |
| − | Liquid culture of transformed bacteria and induction of protein expression<br><br><br><br><br><br><br><br>For the detailed protocols followed, refer to the PDF documents below
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − | Operators: Juliette
| + | |
| − | <br>
| + | |
| − | Digestion if pSB1C3 by EcoRI and PstI
| + | |
| − | <br>
| + | |
| − | DNA Electrophoresis of pSB1C3 digested by EcoRI-PsTI<br><br><br><br><br><br><br><br>For the detailed protocols followed, refer to the PDF documents below
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 8
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − | Operators: Paul and Azéline
| + | |
| − | <br>
| + | |
| − | DNA Electrophoresis of S1 digested by EcoRI-PstI or XbaI-BamHI<br><br><br><br><br><br><br><br>For the detailed protocols followed, refer to the PDF documents below
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | | + | |
| − | </tr><tr>
| + | |
| − | | + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 9
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 10
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − | Operators: Juliette and Alexis
| + | |
| − | <br>
| + | |
| − | Gel Extraction with QIAquick Gel Extraction Kit of pSB1C3, S1 E-P and S1 X-B
| + | |
| − | <br><br>
| + | |
| − | Ligation of pSB1C3 with our parts E1_1, E1_2, E2, E3, E4, S1
| + | |
| − | <br><br>
| + | |
| − | DH5α Transformation with previous plasmid<br><br><br><br><br><br><br><br>For the detailed protocols followed, refer to the PDF documents below
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 11
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − | Operators: Juliette
| + | |
| − | <br><br>
| + | |
| − | Ligation of pET32a with S1
| + | |
| − | <br><br>
| + | |
| − | Transformation of DH5α with pET32a-S1 <br><br><br><br><br><br><br><br>For the detailed protocols followed, refer to the PDF documents below
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 12
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 13
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 14
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 15
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | | + | |
| − | </tr><tr>
| + | |
| − |
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 16
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 17
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 18
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 19
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − | Operators: Gabriel
| + | |
| − | <br>
| + | |
| − | Digestion of Biobricks made the 10-10-2017<br><br><br><br><br><br><br><br>For the detailed protocols followed, refer to the PDF documents below
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 20
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 21
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 22
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | | + | |
| − | </tr><tr>
| + | |
| − |
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 23
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 24
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − | Operators: Paul
| + | |
| − | <br>
| + | |
| − | Liquid culture of DH5α with all of our biobricks for -80°C storage<br><br><br><br><br><br><br><br>For the detailed protocols followed, refer to the PDF documents below
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 25
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 26
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − | Operators : Juliette, Gabriel, Ersin
| + | |
| − | <br><br>
| + | |
| − | Reaction kinetics of the degradation of Anthracene<br><br><br><br>Please check our Demonstrate page for further information<br><br><br><br>For the detailed protocols followed, refer to the PDF documents below
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 27
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − | Operators : Juliette, Gabriel, Ersin
| + | |
| − | <br><br>
| + | |
| − | Reaction kinetics of the degradation of Anthracene<br><br><br><br>Please check our Demonstrate page for further information<br><br><br><br>For the detailed protocols followed, refer to the PDF documents below
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 28
| + | |
| − | <div class="caseadroitetext1">
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| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
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| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 29
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | | + | |
| − | </tr><tr>
| + | |
| − |
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 30
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <div class=Jours>
| + | |
| − | 31
| + | |
| − | <div class="caseadroitetext1">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | <div class="caseadroitetext2">
| + | |
| − | <br><br><br><br>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | </table>
| + | |
| − | | + | |
| − | | + | |
| − | <br><br>For blank protocols, please check our protocol page <a href="https://2017.igem.org/Team:Pasteur_Paris/Protocol"><span style="color:#3298CB;">here</span></a><br><br><br>
| + | |
| − | For the link between our Biobricks and the abreviations used in the protocols, please check our parts page <a href="https://2017.igem.org/Team:Pasteur_Paris/Parts"><span style="color:#3298CB;">here</span></a><br><br><br><br>
| + | |
| | </div> | | </div> |
| − |
| |
| − | <div class="caseadroitetext3">
| |
| − | <div class="maintextsuite" style="font-size: 15pt; margin-left:0px;">Week 1 protocols</div>
| |
| − | <ul style="font-size: 10pt; font-family:Helvetica;" >
| |
| − | <a href="https://static.igem.org/mediawiki/2017/6/6a/T--Pasteur_Paris--20170705.pdf" target=_blank style="color:black"style="color:black">20170705</a><br>
| |
| − | <a href="https://static.igem.org/mediawiki/2017/c/c7/T--Pasteur_Paris--20170706.pdf" target=_blank style="color:black">20170706</a><br>
| |
| − | <a href="https://static.igem.org/mediawiki/2017/2/20/T--Pasteur_Paris--20170707.pdf" target=_blank style="color:black">20170707</a><br>
| |
| − | </ul><br><br><br><br>
| |
| − | <div class="maintextsuite" style="font-size: 15pt; margin-left:0px;">Week 2 protocols</div>
| |
| − | <ul style="font-size: 10pt; font-family:Helvetica;" >
| |
| − | <a href="https://static.igem.org/mediawiki/2017/3/35/T--Pasteur_Paris--20170711.pdf" target=_blank style="color:black">20170711</a><br>
| |
| − | <a href="https://static.igem.org/mediawiki/2017/e/e8/T--Pasteur_Paris--20170712.pdf" target=_blank style="color:black">20170712</a><br>
| |
| − | <a href="https://static.igem.org/mediawiki/2017/b/bd/T--Pasteur_Paris--20170713.pdf" target=_blank style="color:black">20170713</a><br>
| |
| − | </ul><br><br><br><br>
| |
| − | <div class="maintextsuite" style="font-size: 15pt; margin-left:0px;">Week 3 protocols</div>
| |
| − | <ul style="font-size: 10pt; font-family:Helvetica;" >
| |
| − | <a href="https://static.igem.org/mediawiki/2017/f/ff/T--Pasteur_Paris--20170717.pdf" target=_blank style="color:black">20170717</a><br>
| |
| − | <a href="https://static.igem.org/mediawiki/2017/d/db/T--Pasteur_Paris--20170718.pdf" target=_blank style="color:black">20170718</a><br>
| |
| − | <a href="https://static.igem.org/mediawiki/2017/5/5c/T--Pasteur_Paris--20170719.pdf" target=_blank style="color:black">20170719</a><br>
| |
| − | </ul><br><br><br><br>
| |
| − | <div class="maintextsuite" style="font-size: 15pt; margin-left:0px;">Week 4 protocols</div>
| |
| − | <ul style="font-size: 10pt; font-family:Helvetica;" >
| |
| − | <a href="https://static.igem.org/mediawiki/2017/8/8a/T--Pasteur_Paris--20170723.pdf" target=_blank style="color:black">20170723</a><br>
| |
| − | <a href="https://static.igem.org/mediawiki/2017/4/43/T--Pasteur_Paris--20170724.pdf" target=_blank style="color:black">20170724</a><br>
| |
| − | <a href="https://static.igem.org/mediawiki/2017/0/0e/T--Pasteur_Paris--20170725.pdf" target=_blank style="color:black">20170725</a><br>
| |
| − | <a href="https://static.igem.org/mediawiki/2017/c/c3/T--Pasteur_Paris--20170726.pdf" target=_blank style="color:black">20170726</a><br>
| |
| − | </ul><br><br><br>
| |
| − | <div class="maintextsuite" style="font-size: 15pt; margin-left:0px;">Week 5 protocols</div>
| |
| − | <ul style="font-size: 10pt; font-family:Helvetica;" >
| |
| − | <a href="https://static.igem.org/mediawiki/2017/4/47/T--Pasteur_Paris--20170731.pdf" target=_blank style="color:black">20170731</a><br>
| |
| − | <a href="https://static.igem.org/mediawiki/2017/3/38/T--Pasteur_Paris--20170801.pdf" target=_blank style="color:black">20170801</a><br>
| |
| − | <a href="https://static.igem.org/mediawiki/2017/e/e6/T--Pasteur_Paris--20170802.pdf" target=_blank style="color:black">20170802</a><br>
| |
| − | <a href="https://static.igem.org/mediawiki/2017/5/51/T--Pasteur_Paris--20170803.pdf" target=_blank style="color:black">20170803</a><br>
| |
| − | <a href="https://static.igem.org/mediawiki/2017/8/8d/T--Pasteur_Paris--20170804.pdf" target=_blank style="color:black">20170804</a><br>
| |
| − | </ul><br><br>
| |
| − | <div class="maintextsuite" style="font-size: 15pt; margin-left:0px;">Week 6 protocols</div>
| |
| − | <ul style="font-size: 10pt; font-family:Helvetica;" >
| |
| − | <a href="https://static.igem.org/mediawiki/2017/7/71/T--Pasteur_Paris--20170807.pdf" target=_blank style="color:black">20170807</a><br>
| |
| − | <a href="https://static.igem.org/mediawiki/2017/c/c8/T--Pasteur_Paris--20170808.pdf" target=_blank style="color:black">20170808</a><br>
| |
| − | <a href="https://static.igem.org/mediawiki/2017/f/f1/T--Pasteur_Paris--20170809.pdf" target=_blank style="color:black">20170809</a><br>
| |
| − | <a href="https://static.igem.org/mediawiki/2017/2/27/T--Pasteur_Paris--20170810.pdf" target=_blank style="color:black">20170810</a><br>
| |
| − | <a href="https://static.igem.org/mediawiki/2017/0/09/T--Pasteur_Paris--20170811.pdf" target=_blank style="color:black">20170811</a><br>
| |
| − | </ul><br><br>
| |
| − | <div class="maintextsuite" style="font-size: 15pt; margin-left:0px;">Week 7 protocols</div>
| |
| − | <ul style="font-size: 10pt; font-family:Helvetica;" >
| |
| − | <a href="https://static.igem.org/mediawiki/2017/c/cc/T--Pasteur_Paris--20170818.pdf" target=_blank style="color:black">20170818</a><br>
| |
| − | </ul></div>
| |
| − |
| |
| − | <div class="caseadroitetext4">
| |
| − | <div class="maintextsuite2" style="font-size: 15pt; margin-left:0px;">Week 8 protocols</div>
| |
| − | <ul style="font-size: 10pt; font-family:Helvetica;" >
| |
| − | <a href="https://static.igem.org/mediawiki/2017/b/b7/T--Pasteur_Paris--20170821.pdf" target=_blank style="color:black">20170821</a><br>
| |
| − | <a href="https://static.igem.org/mediawiki/2017/f/fc/T--Pasteur_Paris--20170822.pdf" target=_blank style="color:black">20170822</a><br>
| |
| − | <a href="https://static.igem.org/mediawiki/2017/b/b5/T--Pasteur_Paris--20170823.pdf" target=_blank style="color:black">20170823</a><br>
| |
| − | </ul><br><br><br><br>
| |
| − | <div class="maintextsuite2" style="font-size: 15pt; margin-left:0px;">Week 9 protocols</div>
| |
| − | <ul style="font-size: 10pt; font-family:Helvetica;" >
| |
| − | <a href="https://static.igem.org/mediawiki/2017/1/1b/T--Pasteur_Paris--20170829.pdf" target=_blank style="color:black">20170829</a><br>
| |
| − | <a href="https://static.igem.org/mediawiki/2017/2/2d/T--Pasteur_Paris--20170830.pdf" target=_blank style="color:black">20170830</a><br>
| |
| − | </ul><br><br><br><br><br>
| |
| − | <div class="maintextsuite2" style="font-size: 15pt; margin-left:0px;">Week 10 protocols</div>
| |
| − | <ul style="font-size: 10pt; font-family:Helvetica;" >
| |
| − | <a href="https://static.igem.org/mediawiki/2017/4/44/T--Pasteur_Paris--20170906.pdf" target=_blank style="color:black">20170906</a><br>
| |
| − | <a href="https://static.igem.org/mediawiki/2017/9/94/T--Pasteur_Paris--20170907.pdf" target=_blank style="color:black">20170907</a><br>
| |
| − | </ul><br><br><br><br><br>
| |
| − | <div class="maintextsuite2" style="font-size: 15pt; margin-left:0px;">Week 13 protocols</div>
| |
| − | <ul style="font-size: 10pt; font-family:Helvetica;" >
| |
| − | <a href="https://static.igem.org/mediawiki/2017/8/8e/T--Pasteur_Paris--20170928.pdf" target=_blank style="color:black">20170928</a><br>
| |
| − | </ul><br><br><br><br><br><br>
| |
| − | <div class="maintextsuite2" style="font-size: 15pt; margin-left:0px;">Week 14 protocols</div>
| |
| − | <ul style="font-size: 10pt; font-family:Helvetica;" >
| |
| − | <a href="https://static.igem.org/mediawiki/2017/0/07/T--Pasteur_Paris--20171005.pdf" target=_blank style="color:black">20171005</a><br>
| |
| − | <a href="https://static.igem.org/mediawiki/2017/4/42/T--Pasteur_Paris--20171007.pdf" target=_blank style="color:black">20171007</a><br>
| |
| − | <a href="https://static.igem.org/mediawiki/2017/7/71/T--Pasteur_Paris--20171008.pdf" target=_blank style="color:black">20171008</a><br>
| |
| − | </ul><br><br><br><br>
| |
| − | <div class="maintextsuite2" style="font-size: 15pt; margin-left:0px;">Week 15 protocols</div>
| |
| − | <ul style="font-size: 10pt; font-family:Helvetica;" >
| |
| − | <a href="https://static.igem.org/mediawiki/2017/b/bb/T--Pasteur_Paris--20171010.pdf" target=_blank style="color:black">20171010</a><br>
| |
| − | <a href="https://static.igem.org/mediawiki/2017/f/f5/T--Pasteur_Paris--20171011.pdf" target=_blank style="color:black">20171011</a><br>
| |
| − | </ul><br><br><br><br><br>
| |
| − | <div class="maintextsuite2" style="font-size: 15pt; margin-left:0px;">Week 16 protocols</div>
| |
| − | <ul style="font-size: 10pt; font-family:Helvetica;" >
| |
| − | <a href="https://static.igem.org/mediawiki/2017/4/49/T--Pasteur_Paris--20171019.pdf" target=_blank style="color:black">20171019</a><br>
| |
| − | </ul><br><br><br><br><br>
| |
| − | <div class="maintextsuite2" style="position:relative; font-size: 15pt; margin-left:0px; left: -13vw">Week 17 protocols</div>
| |
| − | <ul style="font-size: 10pt; font-family:Helvetica; position:relative; left: -12vw">
| |
| − | <a href="https://static.igem.org/mediawiki/2017/5/5b/T--Pasteur_Paris--20171026-20171027.pdf" target=_blank style="color:black">20171026</a><br>
| |
| − | <a href="https://static.igem.org/mediawiki/2017/5/5b/T--Pasteur_Paris--20171026-20171027.pdf" target=_blank style="color:black">20171027</a><br>
| |
| − | </ul></div>
| |
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