Difference between revisions of "Team:Pasteur Paris/Protocol"
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<a href="https://2017.igem.org/Team:Pasteur_Paris/Parts">Parts</a> | <a href="https://2017.igem.org/Team:Pasteur_Paris/Parts">Parts</a> | ||
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</div> | </div> | ||
Latest revision as of 21:35, 15 December 2017
Protocols
Agarose Gel Preparation
Bacteria Transformation
Bacteria Transformation (Interlab)
Dephosphorylation of DNA
DNA Digestion
DNA Electrophoresis and Gel Extraction
Ligation of Plasmid with DNA Insert
Liquid Culture and Induction of Protein Expression
Liquid Culture for a Miniprep on Transformed Bacteria
Measurement of DNA Concentration
Miniprep
Small Scale Bacteria Lysis and SDS PAGE Gel Migration
During our lab work, we used the following protocols. You can download them all.
