Difference between revisions of "Team:TU Darmstadt"

 
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   <center> <a href="https://2017.igem.org/Team:TU_Darmstadt/Demonstrate">  
 
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   <h2 style="color:white">> Proof of Concept <</h2></a> </center>
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<div class="4u"><span class="image fit"><a href="https://2017.igem.org/Team:TU_Darmstadt/project/hydrogel"><img src="https://static.igem.org/mediawiki/2017/e/e3/T--TU_Darmstadt--Teaser_Pic4.png" style="border-radius:50%; z-index: 1; border:3px solid #e4f1fe;" alt=""/></a></span></div>
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<div class="4u"><span class="image fit"><a href="https://2017.igem.org/Team:TU_Darmstadt/project/hydrogel"><img src="https://static.igem.org/mediawiki/2017/e/e3/T--TU_Darmstadt--Teaser_Pic4.png" style="border-radius: 50%;" class="HoverBorder" alt=""/></a></span></div>
 
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<div class="4u"><span class="image fit"><a href="https://2017.igem.org/Team:TU_Darmstadt/project/chitin_synthase"><img src="https://static.igem.org/mediawiki/2017/d/df/T--TU_Darmstadt--Teaser_Pic3.png" style="border-radius:50%; z-index: 1; border:3px solid #e4f1fe;" alt="" /></a></span></div>
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<div class="4u"><span class="image fit"><a href="https://2017.igem.org/Team:TU_Darmstadt/project/chitin_synthase"><img src="https://static.igem.org/mediawiki/2017/d/df/T--TU_Darmstadt--Teaser_Pic3.png" style="border-radius: 50%;" class="HoverBorder" alt="" /></a></span></div>
 
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<div class="4u"><span class="image fit"><a href="https://2017.igem.org/Team:TU_Darmstadt/project/chitin_deacetylase"><img src="https://static.igem.org/mediawiki/2017/c/cf/T--TU_Darmstadt--Teaser_Pic2.png" style="border-radius:50%; z-index: 1; border:3px solid #e4f1fe;" alt="" /></a></span></div>
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<div class="4u"><span class="image fit"><a href="https://2017.igem.org/Team:TU_Darmstadt/project/chitin_deacetylase"><img src="https://static.igem.org/mediawiki/2017/c/cf/T--TU_Darmstadt--Teaser_Pic2.png" style="border-radius: 50%;"  class="HoverBorder" alt="" /></a></span></div>
 
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<div class="4u"><span class="image fit"><a href="https://2017.igem.org/Team:TU_Darmstadt/project/chemistry"><img src="https://static.igem.org/mediawiki/2017/d/db/T--TU_Darmstadt--Teaser_Pic1.png" style="border-radius:50%; z-index: 1; border:3px solid #e4f1fe;" alt="" /></a></span></div>
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<div class="4u"><span class="image fit"><a href="https://2017.igem.org/Team:TU_Darmstadt/project/chemistry"><img src="https://static.igem.org/mediawiki/2017/d/db/T--TU_Darmstadt--Teaser_Pic1.png" style="border-radius: 50%;"  class="HoverBorder" alt="" /></a></span></div>
 
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        <p style="text-align: center;"><a href="http://www.igem.tu-darmstadt.de/igem_2/projekt_2/index.de.jsp">iGEM Technische Universität Darmstadt</a></p>
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        <p style="text-align: center;">We thank our sponsors, instructors and the iGEM organization!</p>
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        <p style="text-align: center;">Design: <a href="http://html5up.net">HTML5 UP</a></p>
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Latest revision as of 20:27, 1 November 2017

MainPage

image/svg+xml 1. Chitosan:Chitosan is a derivate of the linear polysaccharide chitin. The chemical and physical properties can vary remarkably. This enables a huge scope of applications. Chitosan shows antimicrobial properties and supports scar free wound healing.Visit the subpage! 2. Wound infection:Simple and cheap wound treatment is a challenging task.The detection of potentially present pathogenic bacteria iscomplicated, as removal of the wound dressing disrupts thehealing process. 3. Chitin Synthase:To realize the synthesis of chitosan, for usage in wounddressing hydrogels, we produce chitin out of N-acetyl-glucosamine-UDP monomers. Chitin is a precursor for chitosan.We implement the chitin synthase NodC, which createstetrameric and pentameric chitin.Visit the subpage! 4. Chitin Deacetylase:To manufacture chitosan out of chitin, the producedchitin oligomers have to be deacetylated. This can beperformed by using certain hydrolyzing enzymes called chitin deacetylases. In our case,we use NodB and COD.Visit the subpage! 5. Chemistry:To deal with the problem of wound infections andtheir detection, our synthesized chitosan is linkedto a flourophor via a peptide linker. This makes itpossible to detect exoproteases from pathogenicbacteria within minutes.Visit the subpage! image/svg+xml 6. Hydrogel:To use the wound healing supportive properties of chitosan,we manufactured non-toxic, low-cost hydrogels, containingdefined chitosans for usage in wound care.Visit the subpage! 9. CloneCademy:In order to share the knowledge aboutsynthetic biology and the achievementsof our project, we developed a web-basedinteractive learning platform calledCloneCademy. This education tool makesit possible for other iGEM teams to sharetheir ideas with society.Visit the subpage! 7. Solution:By combining the physiological propertiesof chitosan and the novel detectionsystem for wound infections in a hydrogelbandaid, we realized next generation wound care. Furthermore, we successfullyproved all milestones of our project.Visit the subpage! 8. Tech:We constructed a smartphone-adaptable, low-cost and mainly 3Dprinted microscope with a µm resolutionby implementing the digital inlineholographic approach. Thus, allowing the analyzation of ourhydrogel structure during the project. We also presenta software solution enabling 3D analyzation based onthe open source HoloPy package.Visit the subpage!


Proof of Concept