Difference between revisions of "Team:UCC Ireland"

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<center style="font-size:24px;font-weight:700;">Bioengineered construct</center>
 
<center style="font-size:24px;font-weight:700;">Bioengineered construct</center>
 
A biological circuit designed to detect the presence of a specific substrate. The sensing element is specific to the substance being detected, while the readout for each construct is AmilCP, a blue coloured Chromoprotein.
 
A biological circuit designed to detect the presence of a specific substrate. The sensing element is specific to the substance being detected, while the readout for each construct is AmilCP, a blue coloured Chromoprotein.
 
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<img class="homepage_into_img" src='https://static.igem.org/mediawiki/2017/b/b2/T--UCC_Ireland--amilcp.png'>
 
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<center style="font-size:24px;font-weight:500;">Chassis</center>
 
<center style="font-size:24px;font-weight:500;">Chassis</center>
 
2. Chassis
 
2. Chassis
 
The system is deployed initially through plasmid containing E. coli (tube-based), with the aim of progressing to a cell free system (as a lyophilised paper system).
 
The system is deployed initially through plasmid containing E. coli (tube-based), with the aim of progressing to a cell free system (as a lyophilised paper system).
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<center style="font-size:24px;font-weight:500;">Readout device</center>
 
<center style="font-size:24px;font-weight:500;">Readout device</center>
 
3. Readout Device
 
3. Readout Device
 
The readout device has been 3D printed and is designed to attach to common smartphones. The device acts as a spectrophotometer, enabling easy measurement of the relative fluorescence or absorbance of a sample. Using a mobile app and algorithms developed by the team, the device compares the fluorescence/absorbance measurement to a standard curve and determine the concentration of the substance being tested for in the sample.
 
The readout device has been 3D printed and is designed to attach to common smartphones. The device acts as a spectrophotometer, enabling easy measurement of the relative fluorescence or absorbance of a sample. Using a mobile app and algorithms developed by the team, the device compares the fluorescence/absorbance measurement to a standard curve and determine the concentration of the substance being tested for in the sample.
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Revision as of 22:07, 1 November 2017

UCC iGEM 2017

Our project aims to create a standardised universal biosensor strategy, that will be affordable and user friendly, containing all the components for readout. This system will consist of three elements: (1) a bioengineered construct, (2) the chassis and (3) the readout device.

Bioengineered construct
A biological circuit designed to detect the presence of a specific substrate. The sensing element is specific to the substance being detected, while the readout for each construct is AmilCP, a blue coloured Chromoprotein.
Chassis
2. Chassis The system is deployed initially through plasmid containing E. coli (tube-based), with the aim of progressing to a cell free system (as a lyophilised paper system).
Readout device
3. Readout Device The readout device has been 3D printed and is designed to attach to common smartphones. The device acts as a spectrophotometer, enabling easy measurement of the relative fluorescence or absorbance of a sample. Using a mobile app and algorithms developed by the team, the device compares the fluorescence/absorbance measurement to a standard curve and determine the concentration of the substance being tested for in the sample.
To validate the idea for our Universal Biosensor Strategy, we identified irish industries with a need for such a product. We have targeted the dairy and microbrewery industries as proof of concept for our project, to test for antibiotic or methanol residue.