Difference between revisions of "Team:UNOTT/Modelling"

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       <h1><a href="http://">Modeling</a></h1>
 
       <h1><a href="http://">Modeling</a></h1>
 
       <p style="text-align: center;">All our models and simulations over the course of our project.</p>
 
       <p style="text-align: center;">All our models and simulations over the course of our project.</p>
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<h5> It was decided that the central aim for the modeling would be to write a simulation for the wet lab to use to show what they can expect during the construction of the bacteria. The parameters that were decided for the model would be the protein concentrations and wavelength of the lasers that would enter the culture.</h5>
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<a href="https://github.com/BurgundyIsAPublicEnemy/iGEMNotts2017/blob/master/LuciferA.c">Download our source code from our gitHub page</a>
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     </main>
 
     </main>
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         <h2>Predicting Fluoresce Intensity and Wavelengths Given Parameters</h2>
 
         <h2>Predicting Fluoresce Intensity and Wavelengths Given Parameters</h2>
 
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<h5> It was decided that the central aim for the modeling would be to write a simulation for the wet lab to use to show what they can expect during the construction of the bacteria. The parameters that were decided for the model would be the protein concentrations and wavelength of the lasers that would enter the culture.</h5>
 
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<a href="https://github.com/BurgundyIsAPublicEnemy/iGEMNotts2017/blob/master/LuciferA.c">Download our source code from our gitHub page</a>
 
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<h2> Constitutive Gene Expression For Protein and mRNA Expression over Time </h2>
 
<h2> Constitutive Gene Expression For Protein and mRNA Expression over Time </h2>
 
<h5>Assuming the gene expression was unregulated and the gene is always on, the constitutive gene expression model was used to guide the model.</h5>
 
<h5>Assuming the gene expression was unregulated and the gene is always on, the constitutive gene expression model was used to guide the model.</h5>

Revision as of 21:04, 2 September 2017

Modeling

All our models and simulations over the course of our project.

It was decided that the central aim for the modeling would be to write a simulation for the wet lab to use to show what they can expect during the construction of the bacteria. The parameters that were decided for the model would be the protein concentrations and wavelength of the lasers that would enter the culture.
Download our source code from our gitHub page
  • Predicting Fluoresce Intensity and Wavelengths
  • MISSION: SYNTHESISING BRICKS
  • MISSION: MUTATING DCAS9
  • MISSION: LIGATING BRICKS
  • MISSION: CONTROL PLASMID TRANSFORMATION

  • Predicting Fluoresce Intensity and Wavelengths Given Parameters

    Constitutive Gene Expression For Protein and mRNA Expression over Time

    Assuming the gene expression was unregulated and the gene is always on, the constitutive gene expression model was used to guide the model.
        $$ sfGFP \underset{Transcriptin}{\rightarrow} mRNA \underset{Translation}{\rightarrow} sfGFP $$ $$ mRNA \underset{Degradation}{\rightarrow} \oslash $$ $$ sfGFP \underset{Degradation}{\rightarrow} \oslash $$
    Using the Law of Mass Action, this model can be described as:
    $$ mRNA = k_{1} -d _{1 } mRNA $$ $$ Protein = k_{2} \cdot mRNA - d_{2} \cdot Protein $$  
    Using this, we can calculate the concentration of proteins we can expect over time. This was useful because it allowed us to see how much protein was made which were emitting the light so we could make an accurate prediction of how much intensity there was at a certain amount of time.

Text

  • MISSION: SYNTHESISING BRICKS

    Text

  • MISSION: MUTATING DCAS9

    Text

  • MISSION: LIGATING BRICKS

    Text

  • MISSION: CONTROL PLASMID TRANSFORMATION

    Text