Difference between revisions of "Team:Warwick/InterLab"

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<h2>Interlab Study</h2>
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<h3><i>How close can the numbers be when fluorescence is measured all around the world?</i></h3>
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<p><strong>Goal:</strong> To establish a GFP measurement protocol based on engineering principles that anyone with a plate reader can use in their lab. Participants of the study were enlisted to produce common, comparable units of measuring GFP with different plate readers. We also tested some RBS devices (BCDs) which are intended to make gene expression more precise and reliable.</p>
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<p>We were supplied with eight plasmids containing the composite parts needed for the study. We also received a Measurement Kit from iGEM HQ, which was used to generate the standard curve needed to measure our parts.</p>
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<a href="https://static.igem.org/mediawiki/2017/d/d6/T--Warwick--2-InterlabProtocol.pdf"><p><strong>Protocol</p></a>
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<p><strong>Results:</strong> Our results can be found here…</p>
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<div class="hwrap"><h4>TARGET DILUTION CALCULATION</h4></div>
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<h1>InterLab</h1>
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<div class="hwrap"><h4>OD600 REFERENCE POINT</h4></div>
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<h3>Bronze Medal Criterion #4</h3>
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<p><b>Standard Tracks:</b> Participate in the Interlab Measurement Study and/or improve the characterization of an existing BioBrick Part or Device and enter this information on that part's Main Page in the Registry. The part that you are characterizing must NOT be from a 2017 part number range.
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<div class="hwrap"><h4>FLUORESCEIN STANDARD CURVE</h4></div>
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For teams participating in the <a href="https://2017.igem.org/Competition/InterLab_Study">InterLab study</a>, all work must be shown on this page.  
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<img src="https://static.igem.org/mediawiki/2017/3/37/T--Warwick--2-FluoresceinStandardCurve2.png" class="img-responsive center-block"/>
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<div class="hwrap"><h4>OD600 REFERENCE POINT</h4></div>
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Latest revision as of 16:39, 1 November 2017

Interlab Study

How close can the numbers be when fluorescence is measured all around the world?


Goal: To establish a GFP measurement protocol based on engineering principles that anyone with a plate reader can use in their lab. Participants of the study were enlisted to produce common, comparable units of measuring GFP with different plate readers. We also tested some RBS devices (BCDs) which are intended to make gene expression more precise and reliable.

We were supplied with eight plasmids containing the composite parts needed for the study. We also received a Measurement Kit from iGEM HQ, which was used to generate the standard curve needed to measure our parts.

Protocol

Results: Our results can be found here…

TARGET DILUTION CALCULATION

OD600 REFERENCE POINT

FLUORESCEIN STANDARD CURVE


OD600 REFERENCE POINT

We thank our sponsors without whom none of this would have been possible: