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<h1 class="headline">Definition of
system specifications</h1>
<p>Determine the target specifications of our bacterial
system regarding the detection levels and azurin production levels</p>
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Retention and Preservation System
...unnatural bases in the DNA requires a cellular retention and preservation system acting on multiple cellular levels. On one hand, degradation of the unnatur
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<h2>Translational
System Overview</h2>
...natural-base pairs (UBP) </a>, the whole transcriptional and translational
system has to be adapted if a <a href="https://2017.igem.org/Team:Bielefeld-CeBiTe
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Retention and Preservation System
...or the <i>in vivo</i> experiments with unnatural base pairs. A two-plasmid system for retention of unnatural base pairs was developed and characterized. In o
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...ystem, as they provide us with comprehensive information on the suppressor system.
...ir/unnatural_base_pairs">unnatural base pairs (UBP)</a>, the translational system enables the recognition of the new bases and the interaction of a matching
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...is incorporated naturally through an endogenous nonsense codon suppression
system(Burke <i>et al.</i>, 1998; Paul <i>et al.</i>, 2000). It was discovered in
...or orthogonality of the exogenous tRNAs towards the endogenous translation
system. The expression of a <a href="http://parts.igem.org/wiki/index.php?titl
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<h2>Translational System Overview</h2>
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...acyl synthetase (aaRS). This could be demonstrated with our CFP‑YFP
system(<a href="http://parts.igem.org/Part:BBa_K2201343">BBa_K2201343</a>)for the
...a low efficiency, thus fewer ncAA are incorporated. Although the screening
system enables to compare the efficiency, a low sfGFP signal in some cases can be
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...he selection of the clones that incorporate the target ncAA. The selection system is based on two selection steps that have to be repeated several times. In
...3 RNA polymerase with a reversed mRFP under T3 promoter control. With this system, the mRFP is expressed, resulting in a red colour of the colonies, still ow
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...org/Team:TU_Darmstadt/project/regulation_system" class="active">Regulatory System</a></li>
<h2>Orthogonal Split T7‑RNA‑Polymerase Regulatory System</h2><!--BIG HEADING-->
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...<li><a href="https://2017.igem.org/Team:CCU_Taiwan/IOT system">IOT system</a></li>
...system in order to transfer data from the device to the app or server. The system contains three parts, the device, the app and the server. The device contai
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...nd requires a considerable amount of wet lab work due to the multi-plasmid system. We aim to build a toolbox for one-step genome engineering based on a stand
...r, the synthesis of the insulin will be controlled by the natural bacteria system of catabolite repression with regulation by a sRNA. At the presence of gluc
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...current. Last but not least, the energy storing unit has a light-dependent system, which activates dormancy during nighttime to reduce energy lost by metabol
...tion was used to shape the entire approach of the light-dependent dormancy system.<br>
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...current. Last but not least, the energy storing unit has a light-dependent
system, which activates dormancy during nighttime to reduce energy lost by metabol
<p>For these experiments, the promoters controlling an RFP reporter
system were cloned into <i>E. coli</i> strain MG1655 on pSB1A2. The cultures were
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...ost complete model, we can change a spontaneously activating
system into a
system with proper signaling behavior by either increasing LuxR degradation, or by
...igem.org/Team:Wageningen_UR/Model/IntegrationQS">separate page</a>. The QS
system depends on a <a href="https://2017.igem.org/Team:Wageningen_UR/Results/Cpx"
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...zation of antigen presence can be achieved. To design an optimal molecular system for Mantis, we have considered three possible visualization strategies (Fig
...on interaction of CpxR and CpxA upon (de)phosphorylation of CpxR by CpxA. System 3) Signal production relies on the release of split YFP upon phosphorylatio
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...v class="row margin-bottom-75 padding-top-125" id="project-design-dormancy-
system" style="margin-top:-125px;"><div class="col-xs-12">
...c/T--SDU-Denmark--zzz-icon.svg" type="image/svg+xml"></object><h2>Dormancy
System</h2></div></div>
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..." style="font-weight:normal; font-size: 0.8em">Cell Free Protein Synthesis System Optimisation</a>
...of glyphosate interactions within the cell, we could not identify a simple system in which a responsive transcription factor was able to affect the productio
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...Used a Design of Experiments approach to successfully optimise a cell-free system</li>
..." style="font-weight:normal; font-size: 0.8em">Cell Free Protein Synthesis System Optimisation</a>
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...y, the basic components of our circuit are a <em>F. oxysporum</em> sensing system, a fungal killing mechanism, and a chassis survival mechanism.
...operators have not yet been identified, making it difficult to adjust the system to <em>E. coli</em>.
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...<a href="#TICS">Two interdependent components system</a>
...ise ratio of the signal detected. For that, a two-interdependent component system spatially separated within two bacterial cell populations (BCP) will be cre
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<h1>SynORI selection system</h1>
<h1>Active partitioning system</h1>
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<br>Added the following ingredients in turn in 30μL system:
<br>put the system into the PCR and set the param:
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<h3>Cumate regulatory system</h3>
<th>Cloning of cumate regulatory system gBlock into pSB1C3</th>
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...information was used during experimental characterisation to optimise our system.<br />
...elled. This design was later used as our proof-of- concept <i>in vitro</i> system.
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<div class="text">MESA two-component
system replication</div>
<div class="desc">Details on the MESA two-component
system, explanation of its relation to our design and the results of its reproduct
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of inputs and the system’s internal state.</strong>
within the circuit[13]. Once the memory system is able to scale up to more states, a cell can be
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...information was used during experimental characterisation to optimise our system.<br />
...and modelled. This design was later used as our proof-of- concept in vitro system.
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<h3>Why do we need a biocontainment system?</h3>
...aim to develop a multilayered strategy for a well-regulated biocontainment system, attempting to minimize the negative impact on the environment and the huma
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...the bacteria. This led our group to look into an antibiotic-free selection system; a number of methods for antibiotic-free selection were brought up. We also
...r evaluating the economic feasibility of implementing our waste-to-plastic system for use in the municipal wastewater treatment plant, the team started to di
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...r system (our new part, pGEME) and inducible E2 killing protein expression system (pGEMF). </p>
...sensitive system - with GFP reporter, and reconstruction of E2 expression system. </p>
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