Team:SCUT-China A/Practice Collabaration
COLLABORATIONS
We mentored a new team SCUT-FSE-CHINA. We shared with them
the experience of how to form an iGEM team, and guided them in the rules, timelines and precautions of
the competition. Besides, we mentored them how to standardize the genes. In return, they assisted us
the design of modeling and help us write some parts of code of the MATLAB.
Linkage to SCUT-FSE-CHINA's Collaborations pages
We met SYSU-Software in April on a meet-up meeting among teams in Southern China. This year we use the Mer-merr bi-direction promoter to sense mercury (Hg) in the environment. If the concentration of Hg transcend a threshold, Hg can repress the repressor of cds of SRRz protein, which will lead to the expression of SRRz protein. Cells lysis process could be detected because the release of the beta-galactosidase which is not able to spread cross the cell membrane at a normal situation will trigger the chromogenic reaction of oNPG whose tube will turn yellow. Since we were not aware of whether the design could work and the approximate range of expression level of the SRRz gene before the experiment, we reached out for them to do a simulation for their genetic circuits.
They built a model for our project using the same algorithm
that utilized in their software simulation section. The following is the circuit we designed for our
project redrawn by their design platform.
For a preciser measurement of the expression of their
gene, SRRz gene, we used GFP as a reporter in this case alternatively. Their model simulated the concentration
of the GFP expressed in our system. The mathematic formula and parameters' and the results are as follows:
The simulation result is as follow:
The real line chart is as follow:
The fitted curve of all the spots generated from real experiment can be seen from here:
From the three figures above, they can prove that their simulation
has the same trend of the real experiment data, which also gave us a meaningful design performance forecast.
Besides, SYSU-Software also troubleshooted the image
processing software of our project whose aim is to get the yellow degree of an experiment tube so that
we can measure the concentration of the SRRz protein. They improved the system behavior and back-end
of the software and gave some advice on during the implementation process.
During the whole collaboration process, we and SYSU-Software
established very closed friendship. We also gave our feedbacks after they got an embryonic form of their
main project. Besides, they also invited us for holding a high school meeting which they organized at
very beginning.
Linkage to SYSU-Software's Collaborations pages
SYSU-CHINA is a friendly and open team. We had a meet up and exchanged our project ideas with each other. Besides, we helped each other to complete or repeat some experiments. We helped SYSU-CHINA team to finish a western blot, and they helped us to repeat the experiment verifying the lysis gene expression by measured the growth curve of our five kinds of engineering bacteria in different concentration of metal ions, which was really very heavy work.
Linkage to SYSU-CHINA's Collaborations pages
Tianjin team came up with the idea that they could gather all the iGEM teams whose projects were about water pollution treatment and built an alliance to unite all information and data concerning the social impact, knowledge and geographical advantages they collected during the conduction of their project. We received their collaboration request via a voice conference and tried our best to help them.
Linkage to Tianjin's Collaborations pages
We joined in CCIC and exchanged our ideas with other iGEMers.
We held iGEM Southern China Regional Meeting with SZU-China and learned a lot from the meeting.
We had a meet-up with PKU and got lots of inspiration from the previous projects.
