Attributions
We would like thank everyone who made this project possible and supported the team in every stage of the process.
Special thanks to:
Daniel Jacobo, Ph.D.
Dr. Daniel Jacobo is the Medicine Department Director at Tecnológico de Monterrey Campus Guadalajara. He helped us evaluate our project, followed up on our advances and contacted the team with different experts. Supported with permissions and requests in laboratories.
Antonio Rentería, Ph.D.
Dr. Rentería is the Engineering Department Director at Tecnológico de Monterrey Campus Guadalajara. He approved and followed up our project, as well as arranged financial support.
MsC. Oscar Osorio
MsC. Oscar Osorio is the Biotechnology Engineering major coordinator and our Secondary P.I. Supported and followed up our project. Helped in problem solving and gave advice on team management.
Arturo Quintero, Ph.D.
Dr. Quintero is our P.I. He supervised our investigation, as well as being in charge of maintaining a collaboration with The CIATEJ (Center for Research and Assistance in Technology and Design of the State of Jalisco).
Luis Figueroa, Ph.D.
Dr. Figueroa is the Synthetic Biology specialist at CIATEJ. He supported the team regarding the Genetic Engineering constructs and taught us bioinformatics, as well as provided laboratory equipment.
Luis Virgen, Ph.D.
Dr. Virgen helped in the ideation of our mathematical model, as well as pointed out key aspects regarding variables and experimental conditions to define it.
Clara Ríos, Ph.D.
Dr. Clara Ríos was a crucial aid in experimentation and definition of enzyme activity in the protocol for testing the enzymes extracted from Aspergillus niger. She suggested improvements to optimize enzyme activity. Gave us throughout assessment in competent cells and transformation protocols.
Alan Gutiérrez
Alan is the Microbiology Laboratory Manager at Tecnológico de Monterrey Campus Guadalajara. He advised us in experimental processes regarding experimental stainings and growth curves of P. putida KT2440, as well as lab equipment usage.
María José Rubio
María José provided the team with laboratory materials and tracked the iGEM kit.
Maria Mercedes Roca, Ph.D.
Dr. Roca initially guided the team and advised us regarding Human Practices and Biosafety considerations. She helped to establish the first contact with CIATEJ and Dr. Figueroa. She also co-imparted The Risk Analysis and Biosafety Workshop organized by Youth Biotech, AMEXBIO (Mexican Society for Biosafety) and Jalisco’s Biosecurity Laboratory.
Luis Ochoa, Ph.D.
Dr. Ochoa is the President of AMEXBIO (Mexican Association for Biosafety). He co-imparted the Risk Analysis and Biosafety Workshop organized by Youth Biotech, AMEXBIO and Jalisco’s Biosecurity Laboratory.
Paulo Paes, Ph.D.
Dr. Paes, from the Federal University of Pernambuco in Brazil, co-imparted The Risk Analysis and Biosafety Workshop organized by Youth Biotech, AMEXBIO (Mexican Society for Biosafety) and Jalisco’s Biosecurity Laboratory.
Yo Suzuki, Ph.D.
Assistant professor and researcher at The J. Craig Venter Institute. He revised the Genetic Engineering constructs and followed up on the project.
José Claudio Moreno, Ph.D.
Dr. Moreno revised the Genetic Engineering constructs and shared with us an open source tool to test them.
MSc. Ricardo Camilo Chávez
MsC. Chávez is one of our Instructors. He guided us regarding G.E. constructs and general considerations of the project. He followed up closely and introduced the team to the iGEM requirements.Continously helped the team and helped revising protocols for characterization of parts
MSc. Heber Torres
MsC. Torres instructed us in vector design and imparted an iGEM Workshop at Monterrey City, Mexico which two of our members attended.
Yolanda Cham
Our presentation advisor and consultant, helped outlining the important aspects of the project for the presentation, advised on how to present data on an non-scientific language. Helped us with objective problem solving.
Alejandro Saldaña
Biotechnology Engineering student from ITESO University. He advised us regarding the bagasse treatment process, experimental procedures as well as contacted us with Dr. Rojas, who helped with the ideation of the bagasse treatment protocol.
Paola Gómez
Biotechnology Engineering student from ITESO University. She advised us regarding the bagasse treatment process, experimental procedures as well as contacted us with Dr. Rojas, who helped with the ideation of the bagasse treatment protocol.
Oscar Ariel Rojas Rejón , Ph.D.
Gave us a guideline for the bagasse treatment process, corrections and theoretical results.
Fernando Viacava, Ph.D.
For bagasse treatment assessment and manual for determining fibers, cellulose, hemicellulose and lignin percentages.
Jorge Benavides, Ph.D.
For his assessment on PHA extraction and purification.
MSc. Luis García
Supervised and gave constant guidance for the creation of the mathematical model.
Ediner Fuentes
Ediner advised us on Environmental Analysis and Agronomy considerations. He also followed up closely our Human Practices and collaboration projects. Helped contacting other experts in Synthetic Biology and Genetic Engineering.
Uriel Barboza
Uriel advised the team regarding general considerations of the project and iGEM requirements.
José Barboza Ph.D.
Helped by sending transformation reagents (from Guanajuato University laboratories) the team was lacking such as SOC medium, cAMP and Mg.
Ricardo Hernández
Ricardo supported the team regarding the first wiki edits.
Alex Mayorov
Natural Sciences student at Cambridge University. Alex helped to solve punctual doubts regarding PHA production, cellulose quantification, and safety forms.
Thomas Dohmen
Masters student at TU Darmstadt in Germany. Advisor for general aspects of the team and facilitator of communications with the TU Darmstadt iGEM team.
Saúl Pizarro
Biotechnology Engineering student at Tecnológico de Monterrey Campus Chihuahua. Saúl advised the team regarding general considerations of the project and iGEM requirements.
Carlos Vázquez
Biotechnology Engineering student at Tecnológico de Monterrey Campus Monterrey. Advised the team in general considerations and iGEM requirements. Participated in initial meetings regarding the funding of the project.
Carla Cárdenas
Biotechnology Engineering student at Tecnológico de Monterrey Campus Guadalajara. She supported the team with investigation work in the early stages of the project.
Luis Fernando Amador
Biotechnology Engineering student at Tecnológico de Monterrey Campus Guadalajara. He supported the team with investigation work in the early stages of the project.
Marlene Aguilar
Digital Arts & Design student at Tecnológico de Monterrey Campus Guadalajara. She helped redesigning our logo, colour palette and assessment in image.
Team member contributions
Human practices
Diana Bonilla
Founding member of Youth Biotech, organization of Youth Biotech events and projects. Completed Safety Forms with P.I. assistance, project abstract & project description. Also, she created the Risk Assessment for the project.
José Ferrer
Education manual development in collaboration with other mexican iGEM teams.
Frida García
Education manual development in collaboration with other mexican iGEM teams.
Mildred Jiménez
Founding member of Youth Biotech, organization of Youth Biotech events and projects, wiki content generation and organization, project justification, socio-economic considerations, history of the project, project abstract.
Santiago Ochoa
Founding member of Youth Biotech, social media manager.
Diana Tamayo
Founding member of Youth Biotech, organization of Youth Biotech events and projects, wiki content generation and organization, project justification, socio-economic considerations, history of the project and helped writing project abstract, helped filling out the last biosafety form.
Diana Rábago
Founding member of Youth Biotech, organization of Youth Biotech events and projects, visits to tequila production plants, social media manager.
Eliel Villegas
Founding member of Youth Biotech, Organization of Youth Biotech events, visits to tequila production plants.
Microbiology
Esmeralda Azano
Pseudomonas putida KT2440 growth kinetics, protocol for cellular lysis for PHA extraction, PHA production mediums.
Diana Bonilla
Pseudomonas putida KT2440 growth kinetics.
Mildred Jiménez
Pseudomonas putida KT2440 growth kinetics, cell culture, protocol for PHA detection.
Ana Cecilia Luque
Pseudomonas putida KT2440 growth kinetics and investigation, cell culture.
Ana Isabel Montalvo
Pseudomonas putida KT2440 growth kinetics and investigation, cell culture, stainings.
Santiago Ochoa
Pseudomonas putida KT2440 growth kinetics.
Diana Tamayo
Pseudomonas putida KT2440 growth kinetics and investigation, cell culture, protocol for PHA detection, bacterial characterization & stainings.
Diana Rábago
Pseudomonas putida KT2440 growth kinetics.
Eliel Villegas
Pseudomonas putida KT2440 growth kinetics.
Montserrat Villegas
Pseudomonas putida KT2440 growth kinetics, protocol for cellular lysis for PHA extraction, PHA production mediums.
Bagasse treatment
Esmeralda Azano
Agave leaves treatment experiment.
Yatzil Guadalajara
Bagasse experimentation for cellulose extraction, cellulase quantification, cellulase extraction.
Mildred Jiménez
Bagasse experimentation for cellulose extraction.
Ana Cecilia Luque
Obtained bagasse, bagasse hydrolysis, enzymatic tests, experimentation for glucose extraction. Also got the bagasse.
Ana Isabel Montalvo
Bagasse experimentation for glucose extraction, enzymatic tests.
Santiago Ochoa
Bagasse experimentation, bagasse setup for hidrolisis.
Diana Tamayo
Bagasse experimentation for cellulose extraction.
Diana Rábago
Bagasse experimentation for cellulose extraction.
Montserrat Villegas
Bagasse experimentation for cellulose extraction.
Estefanía Verdugo
Bagasse treatment.
Genetic engineering
Diana Bonilla
Vector construct & design, attended iGEM course imparted by Heber Torres in Monterrey, Mexico. Carried out G.E. work planning at CIATEJ.
Mildred Jiménez
Carried out G.E. work planning at CIATEJ.
Santiago Ochoa
Vector design, attended iGEM course imparted by Heber Torres in Monterrey, Mexico. Carried out G.E. work planning at CIATEJ.
Diana Tamayo
Carried out G.E. work planning at CIATEJ .
Stephany Verdugo
Assisted
Mathematicl model
Esmeralda Azano
Mathematical model investigation & design.
Yatzil Guadalajara
Mathematical model investigation & design.
Diana Rábago
Mathematical model investigation & design.
Inter lab
Diana Bonilla
Competent cells production and transformation.
Mildred Jiménez
Competent cells production and transformation.
Santiago Ochoa
Competent cells production and transformation.
Diana Tamayo
Competent cells production and transformation.
Frida García
Competent cells production and transformation.
Characterization of existing part
Diana Bonilla
Proposed a part and a method for its characterization.Characterization participation in wet lab.
Mildred Jiménez
Carried out characterization.
Santiago Ochoa
Proposed a part and a method for its characterization, carried out the characterization.
Diana Tamayo
Proposed different parts and a methods for their characterization, reviewed and obtained materials and protocols, carried out the characterization.
PHA extraction and characterization
Esmeralda Azano
Developed environmental friendly protocol for PHA extraction, characterized PHA produced by Pseudomonas putida KT2440.
Montserrat Villegas
Developed environmental friendly protocol for PHA extraction, characterized PHA produced by Pseudomonas putida KT2440.
Diana Tamayo
Protocol for detecting PHA in bacterial cells with Nile Blue plate staining and microscopy staining with Sudan B and Nile Blue, as well as carrying it out
Collaborations
José Ferrer
Education manual development in collaboration with other mexican iGEM teams.
Frida García
Education manual development in collaboration with other mexican iGEM teams. Lab collaborations.
Diana Tamayo
Lab collab Chihuahua
Diana Bonilla
Lab collab Chihuahua
Santiago Ochoa
Lab collab Chihuahua
Mildred Jiménez
Lab collab Chihuahua
Ana Isabel Montalvo
Lab collab CDMX
Ana Cecilia Luque
Lab collab CDMX
Stephany Verdugo
Lab collab CDMX
Yatzil Guadalajara
Lab collab CDMX
Finances
Diana Rábago
Project budget, campaign for science support.
Eliel Villegas
Project budget, helped with initial organization of the team and finances, designed the project’s business model. Lab collab Chihuahua.
