Team:UCC Ireland/Project/Notebook/03/07

Monday-03/07/17

Transformation of:

• BBa_c0040 - Plasmid with TetR gene
• BBa_I13600 - Plasmid with cfp reporter
• BBa_J23119 - Plasmid with constitutive promoter
• BBa_K1357008 - Plasmid with purple fluorescent protein
• BBa_K1357009 - Plasmid with amilCP gene

3A assembly method

Standard assembly method

Tuesday-04/07/17

Overnight cultures of the transformations

Wednesday-05/07/17

Meeting with Dr. Paul Young

Thursday-06/07/17

• Miniprep of TetR plasmid.
• Restriction digest using EcoR1 and Pst1.
• Control without enzymes

^Ladder. Restriction digest. Plasmid only.