Team:UCC Ireland/Project/Notebook/07/08

Monday-07/08/17

Tuesday-08/08/17

• Made TE buffer
• Resuspended Mdh G-block in 83 mcl of TE buffer and incubated it for 20 minutes at 50
• Final concentration of Mdh G-block was 9.8ng/mcl
• Made new 5% M9 overnights (500microl in 10ml)
• Made new Amp plates
• Analysed sequence of TetR-pTet-RFP ligation - 3A assembly
• Primers ordered

Wednesday-09/08/17

• Checked overnights, varying growth in the new generation
• Meeting; make up dilutions of overnights and measure cell count to relate cell count to intensity of colour from the amilCP relate colour concentration

Thursday-10/08/17

• IVIS training
• Tested cell counts
• Prepared other Gblocks

Friday-11/08/17