Team:USTC/Basic Part

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This year we, team USTC, submitted 5 basic parts. All of them are coding part as we did not use any special promoters or RBS in our project. Among these five basic parts, two of them are new, including reductase CmCR and KmAdh.

Part’s Number	Part’s Name	Type	Length	Function	Source	Safety Issues
BBa_K2242363	Mtr CAB	Coding	5210	It consists of 3 proteins, Mtr A, Mtr B and Mtr C, which plays the role as electron tunnel to transfer extracellular electrons into the cytoplasm.	It comes from the kit plate from iGEM 2017.
BBa_K2242666	Ccm A-H	Coding	6290	Its full name is Cytochrome c Maturation, which can mature Mtr CAB and enable it to function as we expected.	We cloned this gene ccm A-H from the genome of an E.coli strain BL21(DE3).
BBa_K2242233	CysDes	Coding	1203	Cysteine Desulfhydrase is an aminotransferase that converts cysteine into pyruvate, ammonia, and hydrogen sulfide. In our project, we use it to generate Cd²⁺	Synthesized by IDT.	As this aminotransferase can produce hydrogen sulfide and CdS nanoparticles if S^2-is in the system, special measures must be taken.
BBa_K2242180	Reductase CmCR	Coding	873	This reductase CmCR is a NADH-dependent reductase which can catalyze the bio-transformation from COBE to (S)-CHBE.	Kindly provide by Prof.Hong Jiong	As COBE is harmful to our skin and respiratory tract with pungent smell, special measures must be taken.
BBa_K2242626	Reductase KmAdh	Coding	888	KmAdh is an alcohol dehydrogenase from Kluyveromyces marxianus. It can catalyze the ethanol fermentation ongoing in the bacteria with a high efficiency and mild reaction condition, especially temperature.	Kindly provide by Prof.Hong Jiong