Team:USTC/Composite Part

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This year we, team USTC, submitted 7 basic parts. All of them are coding part as we did not use any special promoters or RBS in our project. These parts are different proteins that we have used in our project under control of different promoter or operator.

Part’s Number	Part’s Name	Type	Length	Function	Source	Safety Issues
BBa_K2242920	araC+pBAD+CmCR	Coding	2089	To induce the gene expression, we use a promotor called pBAD, which is induced by arabinose.	The plasmid is bought from a bio-company. The reductase CmCR’s gene is kindly provided by Prof.HongJiong. The recombinant plasmid is constructed by ourselves.	The substrate of this reductase is COBE, which is an organic compound with a pungent smell. More importantly, it’s harmful to our skin and respiratory tract. So during the experiment process, we need to take up certain measures to protect ourselves.
BBa_K2242521	placI+lacI+pTAC+CmCR	Coding	2326	We use a promoter pTAC to control the gene CmCR’s expression, which is induced by IPTG.	The plasmid backbone pYYDT is kindly provided by Prof. Yu Hanqing. The gene CmCR is kindly provided by Prof. Hong Jiong. The recombinant plasmid is constructed by ourselves.	The substrate of this reductase is COBE, which is an organic compound with a pungent smell. More importantly, it’s harmful to our skin and respiratory tract. So during the experiment process, we need to take up certain measures to protect ourselves.
BBa_K2242018	T7+LacO+Mtr	Coding	5272	We use T7 promoter and Lac operator to control Mtr CAB’s expression. Both of this two units are induced by IPTG. With this dual switch, we can reduce the leak of the gene expression as much as possible.	The plasmid is bought from a bio-company. The Mtr gene is obtained from the kit plate. The recombinant plasmid is constructed by ourselves.
BBa_K2242419	pTET+ccm	Coding	6372	We use a constitutively on promoter pTET to control gene ccmA-H’s expression.	The plasmid backbone and the promoter pTET is from the kit plate. The ccm A-H gene is amplified from the genome of an E.coli strain BL21(DE3). The recombinant plasmid is constructed by ourselves.
BBa_K2242633	pLuxR+CysDes	Coding	2367	We use the pLuxR promoter from the quorum sensing system, which can be induced by AHL.	The plasmid backbone and the promoter pLuxR is from the kit plate. The CysDes gene is synthesized by IDT. The recombinant plasmid is constructed by ourselves.	As this aminotransferase can produce hydrogen sulfide and CdS nanoparticles if S^2-is in the system, special measures must be taken.
BBa_K2242005	T7+LacO+KmAdh	Coding	948	We use T7 promoter and Lac operator to control KMADH’s expression. Both of this two units are induced by IPTG. With this dual switch, we can reduce the leak of the gene expression as much as possible.	The plasmid is bought from a bio-company. The Mtr gene is kindly provided by Prof.HongJiong. The recombinant plasmid is constructed by ourselves.
BBa_K2242384	placI+lacI+pTAC+KmAdh	Coding	2341	To express this reductase KMADH in Shewallena, we construct a shuttle plasmid. On this plasmid, we use this pTAC to control its expression, which is induced by IPTG.	The plasmid backbone pYYDT is kindly provided by Prof. Yu Hanqing. The gene KMADH is kindly provided by Prof. Hong Jiong. The recombinant plasmid is constructed by ourselves.