Revision as of 03:25, 17 September 2017

2017-06-19   prepare stock1 and stock5
2017-06-20   (1) reprepare stock5
　　　　　(2) prepare BG11 medium
　　　　　(3) unfreeze Synechocystis sp. PCC6803 and start to cultivate in BG11
2017-06-29   (1) prepare Pseudomonas aeruginosa growing medium
　　　　　(2) activate Pseudomonas aeruginosa and start to cultivate in Petri dish
　　　　　(3) subculture Synechocystis sp. PCC6803

July, 2017

2017-07-02   extract Pseudomonas aeruginosa plasmid and freeze into refrigerator
2017-07-03   proliferate Pseudomonas aeruginosa and freeze into refrigerator
2017-07-04   (1) prepare BG11 medium
　　　　　(2) proliferate Chlorella vulgaris in Petri dish and centrifuge tube
2017-07-05 to 07-10   cultivate Synechocystis sp. PCC6803 and Chlorella vulgaris in incubator
2017-07-11     (1) extract Synechocystis sp. PCC6803 plasmid
　　　　　(2) prepare LB containing ampicillin and chloramphenicol and prepare Petri dish
2017-07-12   transform BBa_K112806, BBa_K737007, BBa_K112000, BBa_J04450, BBa_K1356003, BBa_K1356004, and BBa_K356005 into competent cell
2017-07-13    (1) proliferate 07-12 transformed E.coli
　　　　　(2) proliferate Chlorella vulgaris
　　　　　(3) proliferate Synechocystis sp. PCC6803
　　　　　(4) activate Synechococcus elongatus PCC7942
2017-07-14    (1)extract 07-13 proliferated E.coli plasmid
　　　　　(2) digestion: BBa_K112806, BBa_K737007, BBa_K112000, BBa_J04450, BBa_K1356003, BBa_K1356004, and BBa_K1356005
　　　　　　(check the correction of transformed E.coli)
2017-07-17    (1) digestion again: BBa_K1356005, BBa_K112806, and BBa_K112000
　　　　　(2) retransform BBa_K112806 and BBa_K112000
2017-07-18    (1) proliferate BBa_K112806 transformed E.coli (BBa_K112000 failed)
　　　　　(2) subculture Synechococcus elongatus PCC7942
　　　　　(3) cultivate Synechocystis sp. PCC6803 in BG11
2017-07-19    (1) extract 07-18 BBa_K112806 transformed E.coli plasmid
　　　　　(2) digestion check
　　　　　(3) save BBa_K112806 transformed E.coli
　　　　　(4) save 07-18 Synechocystis sp. PCC6803
2017-07-20   (1) activate Rhizobium etli in PY medium
　　　　　(2) dilute primer 001~008
　　　　　(3) run NrtA and terminator PCR (failed)
2017-07-21 to 07-23   cultivate Chlorella vulgaris again for measuring growth curve
2017-07-24   (1) prepare Rhizobium etli liquid culture
　　　　　(2) activate Synechococcus elongatus PCC7942
2017-07-25   (1) extract Synechocystis sp. PCC6803 genomic DNA
　　　　　(2) run NrtA PCR
2017-07-26   (1) extract Rhizobium etli genomic DNA
　　　　　(2) run NrtA and terminator PCR
　　　　　(3) run NrtA+ terminator fusion PCR (failed)
2017-07-27   (1) run NrtA+ terminator fusion PCR again
　　　　　(2) purify the successful fusion product
　　　　　(3) digestion check
2017-07-28   run the whole NrtA cloning procedure again, using KOD polymerase
　　　　　(1) run NrtA and terminator PCR
　　　　　(2) digestion check NrtA and terminator and purify the PCR product
　　　　　(3) run NrtA and terminator fusion PCR
　　　　　(4) digestion check and purify the fusion PCR product (failed)
2017-07-29   (1) run NrtA and terminator fusion PCR again
　　　　　(2) digestion check and purify the fusion PCR product
　　　　　(3) ligation: backbone(psb1C3) and promoter, RBS, NrtA, and terminator
　　　　　(4) transformation in to E.coli
2017-07-30   cultivate the transformed E.coli in liquid culture
2017-07-31    (1) extract 07-30 transformed E.coli plasmid
　　　　　(2) restriction enzyme check (failed)

Augest, 2017

2017-06-19 prepare stock1 and stock5

September, 2017

2017-06-19 prepare stock1 and stock5

October, 2017

2017-06-19 prepare stock1 and stock5

Labnote

Download the file

@@ Line 124: / Line 124: @@
 			<h1>Protocol</h1>
 			<h3></h3>
-			<h3>NrtA expression</h3><a herf="">Download the file</a>
+			<h3>NrtA expression</h3>
-			<h3>Suicide gene expression</h3><a herf="">Download the file</a>
+			<p><a herf="">Download the file</a></p>
-			<h3>OD measurement</h3><a herf="">Download the file</a>
+			<h3>Suicide gene expression</h3>
+			<p><a herf="">Download the file</a></p>
+			<h3>OD measurement</h3>
+			<p><a herf="">Download the file</a></p>
 		</div>
@@ Line 132: / Line 135: @@
 			<h1>Timeline</h1>
 			<p></p>
 			<div class='panel'>
 			<div id="s" class="expandable" style='height: 20px;'>
@@ Line 146: / Line 150: @@
 					　　　　　(2) activate Pseudomonas aeruginosa and start to cultivate in Petri dish<br>
 					　　　　　(3) subculture <i>Synechocystis</i> sp. PCC6803
+				</p>
+			</div>
+			</div>
+			<div class='panel'>
+			<div id="s" class="expandable" style='height: 20px;'>
+				<a href="#!" onclick="toggleHeight(this, 240); return false"
+				style="font-family: 'Acme', sans-serif;font-size:28px;color: #479fcc;height: 20px;">
+					July, 2017
+				</a>
+				<p>
+					<b>2017-07-02</b>&nbsp;&nbsp; extract <i>Pseudomonas aeruginosa</i> plasmid and freeze into refrigerator<br>
+					<b>2017-07-03</b>&nbsp;&nbsp; proliferate <i>Pseudomonas aeruginosa</i> and freeze into refrigerator<br>
+					<b>2017-07-04</b>&nbsp;&nbsp; (1) prepare BG11 medium<br>
+					　　　　　(2) proliferate <i>Chlorella vulgaris</i> in Petri dish and centrifuge tube<br>
+					<b>2017-07-05 to 07-10</b>&nbsp;&nbsp; cultivate <i>Synechocystis</i> sp. PCC6803 and <i>Chlorella vulgaris</i> in incubator<br>
+					<b>2017-07-11</b>&nbsp;&nbsp;&nbsp;&nbsp; (1) extract <i>Synechocystis</i> sp. PCC6803 plasmid<br>
+					　　　　　(2) prepare LB containing ampicillin and chloramphenicol and prepare Petri dish<br>
+					<b>2017-07-12</b>&nbsp;&nbsp; transform BBa_K112806, BBa_K737007, BBa_K112000, BBa_J04450, BBa_K1356003, BBa_K1356004, and BBa_K356005 into competent cell<br>
+					<b>2017-07-13</b>&nbsp;&nbsp;&nbsp; (1) proliferate 07-12 transformed E.coli<br>
+					　　　　　(2) proliferate <i>Chlorella vulgaris</i><br>
+					　　　　　(3) proliferate <i>Synechocystis</i> sp. PCC6803<br>
+					　　　　　(4) activate <i>Synechococcus elongatus</i> PCC7942<br>
+					<b>2017-07-14</b>&nbsp;&nbsp;&nbsp; (1)extract 07-13 proliferated E.coli plasmid<br>
+					　　　　　(2) digestion: BBa_K112806, BBa_K737007, BBa_K112000, BBa_J04450, BBa_K1356003, BBa_K1356004, and BBa_K1356005<br>
+					　　　　　　(check the correction of transformed E.coli)<br>
+					<b>2017-07-17</b>&nbsp;&nbsp;&nbsp; (1) digestion again: BBa_K1356005, BBa_K112806, and BBa_K112000<br>
+					　　　　　(2) retransform BBa_K112806 and BBa_K112000<br>
+					<b>2017-07-18</b>&nbsp;&nbsp;&nbsp; (1) proliferate BBa_K112806 transformed E.coli (BBa_K112000 failed)<br>
+					　　　　　(2) subculture <i>Synechococcus elongatus</i> PCC7942<br>
+					　　　　　(3) cultivate <i>Synechocystis</i> sp. PCC6803 in BG11<br>
+					<b>2017-07-19</b>&nbsp;&nbsp;&nbsp; (1) extract 07-18 BBa_K112806 transformed E.coli plasmid<br>
+					　　　　　(2) digestion check<br>
+					　　　　　(3) save BBa_K112806 transformed E.coli<br>
+					　　　　　(4) save 07-18 <i>Synechocystis</i> sp. PCC6803<br>
+					<b>2017-07-20</b>&nbsp;&nbsp; (1) activate Rhizobium etli in PY medium<br>
+					　　　　　(2) dilute primer 001~008<br>
+					　　　　　(3) run NrtA and terminator PCR (failed)<br>
+					<b>2017-07-21 to 07-23</b>&nbsp;&nbsp; cultivate <i>Chlorella vulgaris</i> again for measuring growth curve<br>
+					<b>2017-07-24</b>&nbsp;&nbsp; (1) prepare <i>Rhizobium etli</i> liquid culture<br>
+					　　　　　(2) activate <i>Synechococcus elongatus</i> PCC7942<br>
+					<b>2017-07-25</b>&nbsp;&nbsp; (1) extract <i>Synechocystis</i> sp. PCC6803 genomic DNA<br>
+					　　　　　(2) run NrtA PCR<br>
+					<b>2017-07-26</b>&nbsp;&nbsp; (1) extract <i>Rhizobium etli</i> genomic DNA<br>
+					　　　　　(2) run NrtA and terminator PCR<br>
+					　　　　　(3) run NrtA+ terminator fusion PCR (failed)<br>
+					<b>2017-07-27</b>&nbsp;&nbsp; (1) run NrtA+ terminator fusion PCR again<br>
+					　　　　　(2) purify the successful fusion product <br>
+					　　　　　(3) digestion check<br>
+					<b>2017-07-28</b>&nbsp;&nbsp; run the whole NrtA cloning procedure again, using KOD polymerase<br>
+					　　　　　(1) run NrtA and terminator PCR<br>
+					　　　　　(2) digestion check NrtA and terminator and purify the PCR product<br>
+					　　　　　(3) run NrtA and terminator fusion PCR<br>
+					　　　　　(4) digestion check and purify the fusion PCR product (failed)<br>
+					<b>2017-07-29</b>&nbsp;&nbsp; (1) run NrtA and terminator fusion PCR again<br>
+					　　　　　(2) digestion check and purify the fusion PCR product<br>
+					　　　　　(3) ligation: backbone(psb1C3) and promoter, RBS, NrtA, and terminator<br>
+					　　　　　(4) transformation in to E.coli<br>
+					<b>2017-07-30</b>&nbsp;&nbsp; cultivate the transformed E.coli in liquid culture<br>
+					<b>2017-07-31</b>&nbsp;&nbsp;&nbsp; (1) extract 07-30 transformed E.coli plasmid<br>
+					　　　　　(2) restriction enzyme check (failed)<br>
+				</p>
+			</div>
+			</div>
+			<div class='panel'>
+			<div id="s" class="expandable" style='height: 20px;'>
+				<a href="#!" onclick="toggleHeight(this, 240); return false"
+				style="font-family: 'Acme', sans-serif;font-size:28px;color: #479fcc;height: 20px;">
+					Augest, 2017
+				</a>
+				<p>
+					<b>2017-06-19</b>&nbsp;&nbsp; prepare stock1 and stock5<br>
+				</p>
+			</div>
+			</div>
+			<div class='panel'>
+			<div id="s" class="expandable" style='height: 20px;'>
+				<a href="#!" onclick="toggleHeight(this, 240); return false"
+				style="font-family: 'Acme', sans-serif;font-size:28px;color: #479fcc;height: 20px;">
+					September, 2017
+				</a>
+				<p>
+					<b>2017-06-19</b>&nbsp;&nbsp; prepare stock1 and stock5<br>
+				</p>
+			</div>
+			</div>
+			<div class='panel'>
+			<div id="s" class="expandable" style='height: 20px;'>
+				<a href="#!" onclick="toggleHeight(this, 240); return false"
+				style="font-family: 'Acme', sans-serif;font-size:28px;color: #479fcc;height: 20px;">
+					October, 2017
+				</a>
+				<p>
+					<b>2017-06-19</b>&nbsp;&nbsp; prepare stock1 and stock5<br>
 				</p>
 			</div>
@@ Line 154: / Line 255: @@
 		<div id="Labnote">
 			<h1>Labnote</h1>
+			<p></p>
 			<a herf="https://static.igem.org/mediawiki/2017/7/7d/T--NYMU-Taipei--Labnote001.pdf">Download the file</a>
 			<p></p>

Difference between revisions of "Team:NYMU-Taipei/Notebook"

Revision as of 03:25, 17 September 2017

Protocol

NrtA expression

Suicide gene expression

OD measurement

Timeline

Labnote