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Revision as of 03:38, 11 October 2017
![](https://static.igem.org/mediawiki/2017/f/f9/MARS_Background1.png)
Get Ready to Learn about Microfludics!
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Contents
Microfluidics 101
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What is Microfluidics?
Microfluidics is the manipulation of microlitre volumes of liquid in order to perform scientific experiments. This is performed on devices called microfluidic chips that can be made of polycarbonate, glass or silicone.
What is a Microfluidic Chip?
A continuous flow microfluidic chip typically consists of three layers: a flow layer and control layer with a flexible layer of PDMS between them. There are many other types of microfluidics such as digital or paper which operate using different mechanisms, however we will focus on continuous flow chips as this is the type utilised by MARS.
![Picture](https://static.igem.org/mediawiki/2017/5/5f/LysisFlow.png)
Control Layer
This is the section that will talk about Control layer.
PDMS Layer
This is the section that will talk about PDMS layer.
Flow Layer
These features are called “primitives”. The Control layer allows us to move the elastic PDMS layer in the middle to further manipulate the flow layer, typically by opening or closing valves.
To further understand how primitives work click on the following link: Primitive Table
How does this apply to Synthetic Biology?
Microfluidic chips can be designed to perform various procedures used in synthetic biology. However, microfluidics is often an overlooked tool because designing and using chips requires specialized knowledge.
Primitive Table
Primitive | Design | Mill |
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Channel
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Mixer
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3-D Valve
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Chamber
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Metering
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