Line 15: | Line 15: | ||
background-color:#393a1f; | background-color:#393a1f; | ||
background-image:url('https://static.igem.org/mediawiki/2017/2/24/T--NYMU-Taipei--model_background.png'); | background-image:url('https://static.igem.org/mediawiki/2017/2/24/T--NYMU-Taipei--model_background.png'); | ||
− | |||
} | } | ||
Line 86: | Line 85: | ||
} | } | ||
+ | table, th, td { border: 1px solid black;} | ||
</style> | </style> | ||
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} | } | ||
} | } | ||
+ | |||
+ | |||
</script> | </script> | ||
Line 249: | Line 251: | ||
</h6> | </h6> | ||
− | + | <table> | |
− | + | <tr> | |
− | + | <th></th> | |
− | + | <th scope="col"></th> | |
− | + | <th scope="col"&></th> | |
− | + | <th scope="col"&></th> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <th>R</th> | |
− | + | <th>co2 productive rate</th> | |
+ | <th>mol/g*min </th> | ||
+ | <th>0.000046</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <th>i</th> | ||
+ | <th>irradiance</th> | ||
+ | <th>uE/m^2</th> | ||
+ | <th></th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <th>n</th> | ||
+ | <th> irradiance exponential constant</th> | ||
+ | <th></th> | ||
+ | <th>1.19</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <th>ki</th> | ||
+ | <th>productive coefficient</th> | ||
+ | <th>uE/(m^2)*s</th> | ||
+ | <th>174</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <th>m</th> | ||
+ | <th>constant</th> | ||
+ | <th> (m^2)*s /uE</th> | ||
+ | <th>0.0022</th> | ||
+ | </tr> | ||
+ | </table> | ||
+ | |||
<p></p> | <p></p> | ||
<center> | <center> | ||
Line 273: | Line 304: | ||
R=A1exp(-E1/rT)-A2exp(-E2/rT) | R=A1exp(-E1/rT)-A2exp(-E2/rT) | ||
</h6> | </h6> | ||
− | + | ||
− | + | <table> | |
− | + | <tr> | |
− | + | <th></th> | |
− | + | <th scope="col"></th> | |
− | + | <th scope="col"&></th> | |
− | + | <th scope="col"&></th> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <th>R</th> | |
− | + | <th>co2 productive rate</th> | |
+ | <th></th> | ||
+ | <th></th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <th>A1</th> | ||
+ | <th>preexponential factor at i=400</th> | ||
+ | <th></th> | ||
+ | <th>1147.7</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <th>A2</th> | ||
+ | <th>preexponential factor at i=200</th> | ||
+ | <th></th> | ||
+ | <th>3.818*10^8</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <th>E1</th> | ||
+ | <th>activation energy at i=400</th> | ||
+ | <th>mol/J </th> | ||
+ | <th>42700</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <th>E2</th> | ||
+ | <th>activation energy at i=200</th> | ||
+ | <th>mol/J</th> | ||
+ | <th>77100</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <th>T</th> | ||
+ | <th>temperature</th> | ||
+ | <th>K</th> | ||
+ | <th></th> | ||
+ | </tr> | ||
+ | </table> | ||
<p></p> | <p></p> | ||
Line 364: | Line 429: | ||
<br>Umax = A*exp(-E/RT) | <br>Umax = A*exp(-E/RT) | ||
</h6> | </h6> | ||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
+ | <table> | ||
+ | <tr> | ||
+ | <th></th> | ||
+ | <th scope="col"></th> | ||
+ | <th scope="col"&></th> | ||
+ | <th scope="col"&></th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <th>U</th> | ||
+ | <th>specific growth rate</th> | ||
+ | <th> day^-1</th> | ||
+ | <th></th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <th>Umax</th> | ||
+ | <th>maximum specific growth rate</th> | ||
+ | <th> day^-1</th> | ||
+ | <th></th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <th>Kss</th> | ||
+ | <th>substrate parameter</th> | ||
+ | <th></th> | ||
+ | <th>1</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <th>A</th> | ||
+ | <th>constant</th> | ||
+ | <th> day^-1 </th> | ||
+ | <th>1.0114*10^10</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <th>E</th> | ||
+ | <th>activation energy</th> | ||
+ | <th> cal/mol</th> | ||
+ | <th>6842</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <th>R</th> | ||
+ | <th>gas constant </th> | ||
+ | <th>cal/K*mol </th> | ||
+ | <th>8.314</th> | ||
+ | </tr> | ||
+ | </table> | ||
+ | |||
<p></p> | <p></p> | ||
<center> | <center> | ||
Line 409: | Line 508: | ||
</h6> | </h6> | ||
− | + | <table> | |
− | + | <tr> | |
− | + | <th></th> | |
− | + | <th scope="col"></th> | |
− | + | <th scope="col"&></th> | |
− | + | <th scope="col"&></th> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <th>R</th> | |
− | + | <th>co2 productive rate</th> | |
+ | <th></th> | ||
+ | <th></th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <th>A1</th> | ||
+ | <th>preexponential factor at i=400</th> | ||
+ | <th></th> | ||
+ | <th>8.625*10^-5</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <th>A2</th> | ||
+ | <th>preexponential factor at i=200</th> | ||
+ | <th></th> | ||
+ | <th>1.83885*10^-2</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <th>B1</th> | ||
+ | <th>activation energy at i=400</th> | ||
+ | <th>mol/J </th> | ||
+ | <th>6.45</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <th>B2</th> | ||
+ | <th>activation energy at i=200</th> | ||
+ | <th>mol/J</th> | ||
+ | <th>69.2</th> | ||
+ | </tr> | ||
+ | </table> | ||
+ | |||
<p></p> | <p></p> | ||
<center> | <center> | ||
Line 452: | Line 580: | ||
</h6> | </h6> | ||
− | + | <table> | |
− | + | <tr> | |
− | + | <th></th> | |
− | + | <th scope="col"></th> | |
− | + | <th scope="col"&></th> | |
− | + | <th scope="col"&></th> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <th>Rmax</th> | |
+ | <th>maximum rate</th> | ||
+ | <th>mol/g*min </th> | ||
+ | <th>0.000046</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <th>e</th> | ||
+ | <th>absorbed energy</th> | ||
+ | <th>w/m^2</th> | ||
+ | <th></th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <th>n</th> | ||
+ | <th>energy exponential constant</th> | ||
+ | <th></th> | ||
+ | <th>1.252</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <th>ke</th> | ||
+ | <th>productive coefficient</th> | ||
+ | <th>uE/(m^2)*s</th> | ||
+ | <th>157.88</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <th>m</th> | ||
+ | <th>constant</th> | ||
+ | <th> (m^2)*s /uE</th> | ||
+ | <th>0.0035</th> | ||
+ | </tr> | ||
+ | </table> | ||
<p></p> | <p></p> | ||
Line 498: | Line 655: | ||
<h6 style='color:#bc0101; font-family:"Roboto Mono", monospace;'>ln(Xt/X0)/t=A+Bexp(-C(t-M))=μ(specific growth rate)</h6> | <h6 style='color:#bc0101; font-family:"Roboto Mono", monospace;'>ln(Xt/X0)/t=A+Bexp(-C(t-M))=μ(specific growth rate)</h6> | ||
− | + | <table> | |
− | + | <tr> | |
− | + | <th></th> | |
− | + | <th scope="col"></th> | |
− | + | <th scope="col"&></th> | |
− | + | <th scope="col"&></th> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <th>X</th> | |
− | + | <th>biomass concentration</th> | |
− | + | <th>g/l</th> | |
+ | <th></th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <th>t</th> | ||
+ | <th>time</th> | ||
+ | <th>hr</th> | ||
+ | <th></th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <th>A</th> | ||
+ | <th>the asymptotic of ln Xt/Xo as t decrese indefinitely</th> | ||
+ | <th></th> | ||
+ | <th>1.252</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <th>B</th> | ||
+ | <th>the asymptotic of ln Xt/Xo as t increase indefinitely</th> | ||
+ | <th></th> | ||
+ | <th></th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <th>C</th> | ||
+ | <th>the relative growth rate at time </th> | ||
+ | <th> M</th> | ||
+ | <th></th> | ||
+ | </tr> | ||
+ | </table> | ||
<p></p> | <p></p> | ||
Line 557: | Line 741: | ||
</h6> | </h6> | ||
− | + | <table> | |
− | + | <tr> | |
− | + | <th></th> | |
− | + | <th scope="col"></th> | |
− | + | <th scope="col"& style="border-left:3px solid;"></th> | |
− | + | <th scope="col"&></th> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <th>N</th> | |
− | + | <th width=200>nitrogen</th> | |
− | + | <th style="border-left:3px solid;">X</th> | |
− | + | <th width=200>biomass</th> | |
− | + | <tr> | |
− | + | <th>α</th> | |
− | + | <th width=200>the instantaneous yield coefficient of product formation due to cell growth</th> | |
− | + | <th style="border-left:3px solid;">β</th> | |
− | + | <th width=200>the specific formation rate of product</th> | |
+ | </tr> | ||
+ | <tr> | ||
+ | <th>q</th> | ||
+ | <th width=200>Minimum N quota</th> | ||
+ | <th style="border-left:3px solid;">qM</th> | ||
+ | <th width=200>Maximum N quota</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <th>Q</th> | ||
+ | <th width=200> N quota</th> | ||
+ | <th style="border-left:3px solid;">Vm</th> | ||
+ | <th width=200>Maximum uptake rate of nitrogen</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <th>Vh</th> | ||
+ | <th width=200>Half-saturation coefficient</th> | ||
+ | <th style="border-left:3px solid;">P</th> | ||
+ | <th width=200>lipid</th> | ||
+ | </tr> | ||
+ | </table> | ||
+ | |||
<p></p> | <p></p> | ||
<center> | <center> | ||
Line 610: | Line 815: | ||
</h6> | </h6> | ||
− | + | <table> | |
− | + | <tr> | |
− | + | <th></th> | |
− | + | <th scope="col"></th> | |
− | + | <th scope="col"& style="border-left:3px solid;"></th> | |
− | + | <th scope="col"&></th> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <th>n1</th> | |
− | + | <th width=200>biomass at frist state</th> | |
− | + | <th style="border-left:3px solid;">n2:</th> | |
− | + | <th width=200>biomass at secind state</th> | |
− | + | <tr> | |
− | + | <th>x</th> | |
− | + | <th width=200>biomass concentration(g/l)</th> | |
− | + | <th style="border-left:3px solid;">t</th> | |
− | + | <th width=200> time(hr)</th> | |
− | + | </tr> | |
+ | </table> | ||
+ | |||
+ | <table> | ||
+ | <tr> | ||
+ | <th></th> | ||
+ | <th scope="col"></th> | ||
+ | <th scope="col"&></th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <th>A</th> | ||
+ | <th>the asymptotic of ln Xt/Xo as t decrese indefinitely</th> | ||
+ | <th>-39.9532</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <th>B</th> | ||
+ | <th>the asymptotic of ln Xt/Xo as t increase indefinitely </th> | ||
+ | <th>-0.0222</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <th>C</th> | ||
+ | <th>the relative growth rate at time M hr</th> | ||
+ | <th>45.6931</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <th>k</th> | ||
+ | <th>constant</th> | ||
+ | <th>8.15229</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <th>b</th> | ||
+ | <th>yield coefficient</th> | ||
+ | <th>1207.569</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <th>ns</th> | ||
+ | <th>initial nitrogen concentration</th> | ||
+ | <th></th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <th>a</th> | ||
+ | <th>regression constant</th> | ||
+ | <th>0.01</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <th>e</th> | ||
+ | <th>a perturbation</th> | ||
+ | <th>0.50678</th> | ||
+ | </tr> | ||
+ | </table> | ||
<p></p> | <p></p> | ||
Line 661: | Line 915: | ||
dn/dt=Yxn*dx/dt+m*x | dn/dt=Yxn*dx/dt+m*x | ||
</h6> | </h6> | ||
− | + | <table> | |
− | + | <tr> | |
− | + | <th></th> | |
− | + | <th scope="col"></th> | |
− | + | <th scope="col"&></th> | |
− | + | <th scope="col"&></th> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <th>n</th> | |
+ | <th>nitrogen concentration</th> | ||
+ | <th> </th> | ||
+ | <th></th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <th>Yxn</th> | ||
+ | <th>nitrate coefficient</th> | ||
+ | <th>g/g</th> | ||
+ | <th> 0.21016</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <th>m</th> | ||
+ | <th>maintenance parameter</th> | ||
+ | <th>hr^-1</th> | ||
+ | <th>0.0014393</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <th>x</th> | ||
+ | <th>biomass concentration</th> | ||
+ | <th></th> | ||
+ | <th></th> | ||
+ | </tr> | ||
+ | </table> | ||
<p></p> | <p></p> | ||
Line 704: | Line 981: | ||
</h6> | </h6> | ||
− | + | <table> | |
− | + | <tr> | |
− | + | <th></th> | |
− | + | <th scope="col"></th> | |
− | + | <th scope="col"&></th> | |
− | + | <th scope="col"&></th> | |
− | + | </tr> | |
− | + | <tr> | |
+ | <th>p</th> | ||
+ | <th>lipid concentrtion</th> | ||
+ | <th> </th> | ||
+ | <th></th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <th>K1</th> | ||
+ | <th>growth correlation coefficient</th> | ||
+ | <th>g^2/g^2</th> | ||
+ | <th>122.40085</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <th>K2</th> | ||
+ | <th> non-growth correlation coefficient</th> | ||
+ | <th> g^-1</th> | ||
+ | <th>0.28736</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <th>e</th> | ||
+ | <th> a perturbation</th> | ||
+ | <th>g/l*hr</th> | ||
+ | <th>-0.078</th> | ||
+ | </tr> | ||
+ | </table> | ||
<p></p> | <p></p> | ||
Line 748: | Line 1,049: | ||
</h6> | </h6> | ||
− | + | <table> | |
− | + | <tr> | |
− | + | <th></th> | |
− | + | <th scope="col"></th> | |
− | + | <th scope="col"&></th> | |
− | + | <th scope="col"&></th> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <th>Z</th> | |
− | + | <th>e.coil</th> | |
− | + | <th> </th> | |
− | + | <th></th> | |
− | + | </tr> | |
− | + | <tr> | |
+ | <th>Rx</th> | ||
+ | <th>symbiosis coefficient</th> | ||
+ | <th>g/hr</th> | ||
+ | <th>1.0000023</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <th>Rz</th> | ||
+ | <th>symbiosis coefficient</th> | ||
+ | <th>g/hr</th> | ||
+ | <th>1.178</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <th>Yx</th> | ||
+ | <th> correlation coefficient</th> | ||
+ | <th></th> | ||
+ | <th>12.576</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <th>Yz</th> | ||
+ | <th> correlation coefficient</th> | ||
+ | <th></th> | ||
+ | <th>2.276</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <th>a</th> | ||
+ | <th>population constant</th> | ||
+ | <th></th> | ||
+ | <th>0.80467</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <th>c</th> | ||
+ | <th>population constant</th> | ||
+ | <th></th> | ||
+ | <th>0.61198</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <th>b</th> | ||
+ | <th>relative parameter</th> | ||
+ | <th></th> | ||
+ | <th>0.00027</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <th>g</th> | ||
+ | <th>relative parameter</th> | ||
+ | <th></th> | ||
+ | <th>0.0013</th> | ||
+ | </tr> | ||
+ | </table> | ||
<p></p> | <p></p> | ||
Line 813: | Line 1,162: | ||
</h6> | </h6> | ||
− | + | <table> | |
− | + | <tr> | |
− | + | <th></th> | |
− | + | <th scope="col"></th> | |
− | + | <th scope="col"&></th> | |
− | + | <th scope="col"&></th> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <th>l</th> | |
− | + | <th>lipid proportion in cell</th> | |
+ | <th> </th> | ||
+ | <th></th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <th>k</th> | ||
+ | <th>constant</th> | ||
+ | <th> g/100g</th> | ||
+ | <th>1.13372</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <th>b</th> | ||
+ | <th>yield coefficient</th> | ||
+ | <th></th> | ||
+ | <th>1.57172</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <th>ns</th> | ||
+ | <th>initial nitrogen concentration</th> | ||
+ | <th></th> | ||
+ | <th></th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <th>a</th> | ||
+ | <th> correlation coefficient</th> | ||
+ | <th></th> | ||
+ | <th>2.276</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <th>a</th> | ||
+ | <th>regression constant</th> | ||
+ | <th></th> | ||
+ | <th>0.51653</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <th>e</th> | ||
+ | <th> a perturbation</th> | ||
+ | <th> g/100g</th> | ||
+ | <th>-55.2776</th> | ||
+ | </tr> | ||
+ | |||
+ | </table> | ||
<p></p> | <p></p> |
Revision as of 04:52, 16 October 2017
Modeling is an extremely important part to our project, because it helps us accurately check and predict the results of the experiments, which are worked in the wet lab. In our project, there are two essential types of microalgae that play very important roles, Synechosistic PCC7942andChlorella vulgaris. The following will show our success in modeling.
Synechosistis PCC7942
The modeling from figure 1 to figure 5 belongs to the experiments of Synechosistis PCC7942 pigments.
Chlorella vulgaris
The modeling from figure 6 to figure 12 belongs to the experiments of Chlorella vulgaris nitrogen starvation.
Modeling is an extremely important part to our project, because it helps us accurately check and predict the results of the experiments, which are worked in the wet lab. In our project, there are two essential types of microalgae that play very important roles, Synechosistic PCC7942andChlorella vulgaris. The following will show our success in modeling.
Synechosistis PCC7942
The modeling from figure 1 to figure 5 belongs to the experiments of Synechosistis PCC7942 pigments.
Chlorella vulgaris
The modeling from figure 6 to figure 12 belongs to the experiments of Chlorella vulgaris nitrogen starvation.