Difference between revisions of "Team:HBUT-China/Results"

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{{HBUT-China}}
 
 
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<h1>Results</h1>
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<p>Here you can describe the results of your project and your future plans. </p>
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<script src="http://code.jquery.com/jquery-1.10.2.min.js"></script>
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<h5>What should this page contain?</h5>
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<ul>
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<script type="text/javascript" src="http://123.207.175.242/csdn/js/jq22.js"></script>
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<li> Clearly and objectively describe the results of your work.</li>
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<li> Future plans for the project. </li>
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<li> Considerations for replicating the experiments. </li>
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<h5>You should also describe what your results mean: </h5>
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        <li><img src="https://static.igem.org/mediawiki/2017/5/55/HBUT-logo2.png" height="45"/></li>
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        <li><a href='https://2017.igem.org/Team:HBUT-China'>HOME</a></li>
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<ul>
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        <li class='has-sub'><a href='#'>TEAM</a>
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<li> Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for. </li>
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            <ul>
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<li> Show data, but remember all measurement and characterization data must be on part pages in the Registry. </li>
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              <li ><a href='https://2017.igem.org/Team:HBUT-China/Team'>Team</a></li>
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<li> Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project. </li>
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              <li ><a href='https://2017.igem.org/Team:HBUT-China/Collaborations'>Collaborations</a></li>
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        <li class='has-sub'><a href='javascript:;'>PROJECT</a>
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            <ul>
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              <li ><a href='https://2017.igem.org/Team:HBUT-China/Description'>Description</a></li>
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              <li ><a href='https://2017.igem.org/Team:HBUT-China/Design'>Design</a></li>
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              <li ><a href='https://2017.igem.org/Team:HBUT-China/Experiments'>Experiments</a></li>
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              <li ><a href='https://2017.igem.org/Team:HBUT-China/Notebook'>Notebook</a></li>
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              <li ><a href='https://2017.igem.org/Team:HBUT-China/InterLab'>InterLab</a></li>
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              <li ><a href='https://2017.igem.org/Team:HBUT-China/Model'>Model</a></li>
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              <li ><a href='https://2017.igem.org/Team:HBUT-China/Results'>Results</a></li>
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              <li ><a href='https://2017.igem.org/Team:HBUT-China/Demonstrate'>Demonstrate</a></li>
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              <li ><a href='https://2017.igem.org/Team:HBUT-China/Attributions'>Attributions</a></li>
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            </ul>
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        </li>
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        <li class='has-sub'><a href='javascript:;'>PARTS</a>
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            <ul>
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              <li ><a href='https://2017.igem.org/Team:HBUT-China/Parts'>Parts</a></li>
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              <li ><a href='https://2017.igem.org/Team:HBUT-China/Basic_Parts'>Basic Parts</a></li>
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              <li ><a href='https://2017.igem.org/Team:HBUT-China/Composite_Parts'>Composite Parts</a></li>
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            </ul>
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        </li>
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        <li><a href='https://2017.igem.org/Team:HBUT-China/SAFETY'>SAFETY</a></li>
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        <li class='has-sub'><a href='javascript:;'>HUMAN PRACTICES </a>
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            <ul>
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              <li ><a href='https://2017.igem.org/Team:HBUT-China/Silver_HP'>Silver HP</a></li>
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              <li ><a href='https://2017.igem.org/Team:HBUT-China/Integrated_and_Gold'>Integrated and Gold</a></li>
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              <li ><a href='https://2017.igem.org/Team:HBUT-China/Public_Engagement'>Public Engagement</a></li>
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            </ul>
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        </li>
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      </ul>
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        <a href="javascript:;"><img src="https://static.igem.org/mediawiki/2017/5/55/HBUT-logo2.png" width="300" class="wp1_3 slide1_bot"/></a>               
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<h5> Project Achievements </h5>
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        <span class="txt1">Nickel ion!</span>      
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        <span class="txt2">Heavy metal pollutants!</span>                                 
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<p>You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.</p>
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        <span class="txt3 short">For this year¡¯s iGEM Competition, our team will be focusing on putting forward a biological approach to detect nickel ions as a heavy metal contaminant in the natural environment</span>                                  
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      <div class="oneByOne_item">                                  
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<li>A list of linked bullet points of the successful results during your project</li>
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        <a href="javascript:;"><img src="https://static.igem.org/mediawiki/2017/5/55/HBUT-logo2.png" width="300" class="wp1_3 wp1_left slide2_bot" /></a>                  
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<li>A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.</li>
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        <span class="txt1 blue txt_right2">In order to achieve this goal, we use a nickel-sensitive repressive promoter (pncrA)</span>      
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        <span class="txt1 blue txt_right2">In the absence of nickel ions, the repressor protein (NcrB) binds to the promoter, preventing the expression of the subsequent gene</span>
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              <h1>Result</h1>
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            <h5>We will show our results from three aspects：</h5>
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<small class="bigtitle">Qualitative analysis：</small>
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<h5>After 12 hours of incubation(LB，37℃，200r/min), we observed the bacteria on a fluorescence microscope.</h5>
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<h5>In the medium with the addition of nickel ions, we can see visible red fluorescence.</h5>
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<img src="https://static.igem.org/mediawiki/2017/5/50/Hbut-result-1.png" alt="this is a photo">
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<h5>In other media, there is no such phenomenon. The whole vision is black.</h5>
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<img src="https://static.igem.org/mediawiki/2017/3/3d/Hbut-result-5.png" alt="this is a photo">
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<img src="https://static.igem.org/mediawiki/2017/1/1f/Hbut-result-6.png" alt="this is a photo">
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<h5>So our device only responds to nickel ions.</h5>
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<small class="bigtitle">Quantitative analysis：</small>
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<h5>At the initial induction concentration of OD0.8, and the Induction time is 2 hours.  Under this condition, the nickel ion concentration has a significant function relationship with the fluorescence intensity.</h5>
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<h5>Especially in the nickel ion concentration of 10-6mmol / L to 10-4mmol / L between.</h5>
  
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<div align="center" style="margin:10px 0;">
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<img src="https://static.igem.org/mediawiki/2017/7/71/Hbut-result-7.png" alt="this is a photo">
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<small class="bigtitle">model：</small>
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<h5>At first, we made some assumptions.</h5>
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<h5>Inspiration</h5>
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<small class="bigtitle">Assumptions:</small>
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<p>See how other teams presented their results.</p>
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<p>The degradation of  and other substances are linear.</p>
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<ul>
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<p>Each cell has the same state.</p>
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<li><a href="https://2014.igem.org/Team:TU_Darmstadt/Results/Pathway">2014 TU Darmstadt </a></li>
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<p>The reaction in the process satisfies the first-order kinetic reaction.</p>
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<li><a href="https://2014.igem.org/Team:Imperial/Results">2014 Imperial </a></li>
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<h5>Then we got some conclusions through modeling.</h5>
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<li><a href="https://2014.igem.org/Team:Paris_Bettencourt/Results">2014 Paris Bettencourt </a></li>
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<small class="bigtitle">Incomplete Repression: </small>
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</ul>
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<h5>Under incomplete repression, the bacteria express red fluorescent proteins without the presence of nickel ions.</h5>
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<h5>The incomplete repression of promoters is a major issue in our fluorescence system. This issue was particularly observed in following diagram.</h5>
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<div align="center" style="margin:10px 0;">
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<img src="https://static.igem.org/mediawiki/2017/6/60/8.png" alt="this is a photo">
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</div>
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<h5>The solution of this differential equation is as follows：</h5>
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<div align="center" style="margin:10px 0;">
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<img src="https://static.igem.org/mediawiki/2017/a/a1/Hbut-result-9.png" alt="this is a photo">
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</div>
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<small class="bigtitle">Cytotoxicity:</small>
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<p>We found that a high concentration of nickel ions will reduce the expression of fluorescence, because nickel ions have cytotoxicity. </p>
 +
<p>The July 20 experimental data is as follows:</p>
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<div align="center" style="margin:10px 0;">
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<img src="https://static.igem.org/mediawiki/2017/7/7b/Hbut-result-10.png" alt="this is a photo">
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<p>The different equation of the red fluorescent protein is then modified to:</p>
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<img src="https://static.igem.org/mediawiki/2017/a/a5/Hbut-result-11.png" alt="this is a photo">
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<p>Where</p>
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<img src="https://static.igem.org/mediawiki/2017/e/e2/Hbut-result-12.png" alt="this is a photo">
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<br/><br/><br/><br/>
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  <p>copyright &copy;  2017 IGEM-HBUT-China All rights reserved.</p>
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Revision as of 08:23, 21 October 2017

Result

We will show our results from three aspects：
Qualitative analysis：
After 12 hours of incubation(LB，37℃，200r/min), we observed the bacteria on a fluorescence microscope.
In the medium with the addition of nickel ions, we can see visible red fluorescence.
this is a photo
In other media, there is no such phenomenon. The whole vision is black.
this is a photo
this is a photo
this is a photo
this is a photo
this is a photo
So our device only responds to nickel ions.
Quantitative analysis：
At the initial induction concentration of OD0.8, and the Induction time is 2 hours. Under this condition, the nickel ion concentration has a significant function relationship with the fluorescence intensity.
Especially in the nickel ion concentration of 10-6mmol / L to 10-4mmol / L between.
this is a photo
model：
At first, we made some assumptions.
Assumptions:

The degradation of and other substances are linear.

Each cell has the same state.

The reaction in the process satisfies the first-order kinetic reaction.

Then we got some conclusions through modeling.
Incomplete Repression:
Under incomplete repression, the bacteria express red fluorescent proteins without the presence of nickel ions.
The incomplete repression of promoters is a major issue in our fluorescence system. This issue was particularly observed in following diagram.
this is a photo
The solution of this differential equation is as follows：
this is a photo
Cytotoxicity:

We found that a high concentration of nickel ions will reduce the expression of fluorescence, because nickel ions have cytotoxicity.

The July 20 experimental data is as follows:

this is a photo

The different equation of the red fluorescent protein is then modified to:

this is a photo

Where

this is a photo