Difference between revisions of "Team:USTC/Composite Part"

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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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<h1>Composite Parts</h1>
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<p>
 
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A composite part is a functional unit of DNA consisting of two or more basic parts assembled together. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_I13507">BBa_I13507</a> is an example of a composite part, consisting of an RBS, a protein coding region for a red fluorescent protein, and a terminator.
 
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</p>
 
  
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<p>New composite BioBrick devices can be made by combining existing BioBrick Parts (like Inverters, Amplifiers, Smell Generators, Protein Balloon Generators, Senders, Receivers, Actuators, and so on).</p>
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<h3>Best Composite Part Special Prize</h3>
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<body>
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<header>
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<nav class="top-nav">
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<div class="container">
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<div class="nav-wrapper"><a class="page-title">Basic Parts</a>
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</div>
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</div>
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</nav>
  
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<p>New BioBrick devices can be made by combining existing BioBrick Parts. For example, Inverters, Amplifiers, Smell Generators, Protein Balloon Generators, Senders, Receivers, Actuators, and so on. To be eligible for this award, this part must adhere to <a href="http://parts.igem.org/DNA_Submission">Registry sample submission guidelines</a> and have been sent to the Registry of Standard Biological Parts. If you have a part you wish to nominate your team for this <a href="https://2017.igem.org/Judging/Awards">special prize</a>, make sure you add your part number to your <a href="https://2017.igem.org/Judging/Judging_Form">judging form</a> and delete the box at the top of this page.
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<div class="container">
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<img src="https://static.igem.org/mediawiki/2017/a/a2/Ustc_2017_menu_32px.png">
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<ul id="nav-mobile" class="side-nav fixed">
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<li class="logo" style="border-bottom: 2px solid #ee6e73">
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<div class="logo-container" >
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<img src="https://static.igem.org/mediawiki/2017/thumb/8/89/Ustc_2017team_logo.png/900px-Ustc_2017team_logo.png" width="100%" height="100%">
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</div>
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</li>
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<li class="bold1 lavender_choosed">
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<a href="https://2017.igem.org/Team:USTC" class="waves-effect waves-teal">Home</a>
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</li>
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<br><br>
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<b>Please note:</b> Judges will only look at the first part number you list, so please only enter ONE (1) part number in the judging form for this prize. </p>
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<br>
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<div class="highlight">
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<h4>Note</h4>
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<p>This page should list all the composite parts your team has made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page. Remember judges will only look at the first part in the list for the Best Composite Part award, so put your best part first!</p>
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</div>
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            <li class="bold Aliceblue_choosed"><a class="collapsible-header  waves-effect waves-teal">Project</a>
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              <div class="collapsible-body">
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                <ul>
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                  <li class="bg_all"><a href="https://2017.igem.org/Team:USTC/Description">Description</a></li>
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                  <li class="bg_all"><a href="https://2017.igem.org/Team:USTC/Design">Design</a></li>
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                  <li class="bg_all"><a href="https://2017.igem.org/Team:USTC/Results">Results</a></li>
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                  <li class="bg_all"><a href="https://2017.igem.org/Team:USTC/Demonstrate">Demonstrate</a></li>
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                </ul>
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              </div>
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            </li>
  
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</div>
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            <li class="bold1 azure_choosed">
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<a href="https://2017.igem.org/Team:USTC/Safety" class="waves-effect waves-teal">Safety</a>
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</li>
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<li class="bold1 mintcream_choosed">
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<a href="https://2017.igem.org/Team:USTC/Model" class="waves-effect waves-teal">Model</a>
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</li>
  
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            <li class="bold honeydew_choosed"><a class="collapsible-header  waves-effect waves-teal">Parts</a>
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              <div class="collapsible-body">
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                <ul>
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                  <li><a href="https://2017.igem.org/Team:USTC/Parts">Parts</a></li>
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                  <li><a href="https://2017.igem.org/Team:USTC/Basic_Part">Basic Parts</a></li>
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                  <li><a href="https://2017.igem.org/Team:USTC/Composite_Part">Composite Parts</a></li>
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                  <li><a href="https://2017.igem.org/Team:USTC/Contribution">Contributions</a></li>
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                  <li><a href="https://2017.igem.org/Team:USTC/Improve">Improve</a></li>
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                </ul>
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              </div>
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            </li>
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            <li class="bold ivory_choosed"><a class="collapsible-header waves-effect waves-teal">Notebook</a>
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              <div class="collapsible-body">
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                <ul>
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                  <li><a href="https://2017.igem.org/Team:USTC/Notebook">Experiment Log</a></li>
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                  <li><a href="https://2017.igem.org/Team:USTC/Experiments">Experiments</a></li>
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                </ul>
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              </div>
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            </li>
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            <li class="bold lemonchiffon_choosed"><a class="collapsible-header  waves-effect waves-teal">Human Practice</a>
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              <div class="collapsible-body">
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                <ul>
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                  <li><a href="https://2017.igem.org/Team:USTC/HP/Silver">Silver HP</a></li>
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                  <li style="font-size: 16px"><a href="https://2017.igem.org/Team:USTC/HP/Gold_Integrated">Integrated and Gold</a></li>
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                  <li><a href="https://2017.igem.org/Team:USTC/Engagement">Public Engagement</a></li>
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                </ul>
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              </div>
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            </li>
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            <li class="bold1 cornsilk_choosed">
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<a href="https://2017.igem.org/Team:USTC/Collaborations" class="waves-effect waves-teal">Collaborations</a>
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</li>
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<li class="bold1 seashell_choosed">
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<a href="https://2017.igem.org/Team:USTC/Achievements" class="waves-effect waves-teal">Achievements</a>
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</li>
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<li class="bold1 lavenderblush_choosed">
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<a href="https://2017.igem.org/Team:USTC/Team" class="waves-effect waves-teal">Team</a>
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</li>
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<li class="bold1 mistyrose_choosed">
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<a href="https://2017.igem.org/Team:USTC/Attributions" class="waves-effect waves-teal">Attributions</a>
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</li>
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          </ul>
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        </li>
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<p><br>This year we, team USTC, submitted 5 basic parts. All of them are coding part as we did not use any special promoters or RBS in our project. Among these five basic parts, two of them are new, including reductase CmCR and KmAdh.</p>
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<div >
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                                        <table>
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                                                  <tr>
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                                                      <th>Part’s Number</th>
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                                                      <th>Part’s Name</th>
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                                                      <th>Type</th>
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                                                      <th>Length</th>
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                                                      <th>Short Description</th>
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                                                      <th>Source</th>
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                                                      <th>Safety Issues</th>
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                                                  </tr>
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                                                  <tr>
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                                                    <td><a href="http://parts.igem.org/Part:BBa_K2242920">BBa_K2242920</a></td>
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                                                    <td>araC+pBAD+CmCR</td>
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                                                    <td>Coding</td>
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                                                    <td>2089</td>
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                                                    <td>To induce the gene expression, we use a promotor called pBAD, which is induced by arabinose.</td>
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                                                    <td>The plasmid is bought from a bio-company. The reductase CmCR’s gene is kindly provided by Prof.HongJiong. The recombinant plasmid is constructed by ourselves.</td>
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                                                    <td>The substrate of this reductase is COBE, which is a organic compound with pungent smell. More importantly, it’s harmful to our skin and respiratory tract. So during the experiment process, we need to take up certain measures to protect ourselves.</td>
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                                                  </tr>
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                                                  <tr>
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                                                    <td><a href="http://parts.igem.org/Part:BBa_K2242521">BBa_K2242521</a></td>
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                                                    <td>placI+lacI+pTAC+CmCR</td>
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                                                    <td>Coding</td>
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                                                    <td>2326</td>
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                                                    <td>Its full name is Cytochrome c Maturation, which can mature Mtr CAB and enable it to function as we expected.</td>
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                                                    <td>We cloned this gene ccm A-H from the genome of an E.coli strain BL21(DE3).</td>
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                                                    <td></td>
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                                                  </tr>
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                                                  <tr>
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                                                    <td><a href="http://parts.igem.org/PartBBa_K2242233">BBa_K2242233</a></td>
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                                                    <td>CysDes</td>
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                                                    <td>Coding</td>
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                                                    <td>1203</td>
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                                                    <td>Cysteine Desulfhydrase is an aminotransferase that converts cysteine into pyruvate, ammonia, and hydrogen sulfide. In our project, we use it to generate Cd<sup>2+</sup></td>
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                                                    <td>Synthesized by IDT.</td>
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                                                    <td>As this aminotransferase can produce hydrogen sulfide and CdS nanoparticles if S<sup>2-</sup>is in the system, special measures must be taken.</td>
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                                                  </tr>
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                                                  <tr>
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                                                    <td><a href="http://parts.igem.org/PartBBa_K2242180">BBa_K2242180</td>
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                                                    <td>Reductase CmCR</td>
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                                                    <td>Coding</td>
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                                                    <td>873</td>
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                                                    <td>This reductase CmCR is a NADH-dependent reductase which can catalyze the bio-transformation from COBE to (S)-CHBE.</td>
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                                                    <td>Kindly provide by Prof.Hong Jiong</td>
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                                                    <td>As COBE is harmful to our skin and respiratory tract with pungent smell, special measures must be taken.</td>
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                                                  </tr>
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                                                  <tr>
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                                                    <td><a href="http://parts.igem.org/PartBBa_K2242626">BBa_K2242626</a></td>
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                                                    <td>Reductase KmAdh</td>
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                                                    <td>Coding</td>
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                                                    <td>888</td>
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                                                    <td>KmAdh is an alcohol dehydrogenase from Kluyveromyces marxianus. It can catalyze the ethanol fermentation ongoing in the bacteria with a high efficiency and mild reaction condition, especially temperature.</td>
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                                                    <td>Kindly provide by Prof.Hong Jiong</td>
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Revision as of 07:03, 24 October 2017

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This year we, team USTC, submitted 5 basic parts. All of them are coding part as we did not use any special promoters or RBS in our project. Among these five basic parts, two of them are new, including reductase CmCR and KmAdh.

Part’s Number Part’s Name Type Length Short Description Source Safety Issues
BBa_K2242920 araC+pBAD+CmCR Coding 2089 To induce the gene expression, we use a promotor called pBAD, which is induced by arabinose. The plasmid is bought from a bio-company. The reductase CmCR’s gene is kindly provided by Prof.HongJiong. The recombinant plasmid is constructed by ourselves. The substrate of this reductase is COBE, which is a organic compound with pungent smell. More importantly, it’s harmful to our skin and respiratory tract. So during the experiment process, we need to take up certain measures to protect ourselves.
BBa_K2242521 placI+lacI+pTAC+CmCR Coding 2326 Its full name is Cytochrome c Maturation, which can mature Mtr CAB and enable it to function as we expected. We cloned this gene ccm A-H from the genome of an E.coli strain BL21(DE3).
BBa_K2242233 CysDes Coding 1203 Cysteine Desulfhydrase is an aminotransferase that converts cysteine into pyruvate, ammonia, and hydrogen sulfide. In our project, we use it to generate Cd2+ Synthesized by IDT. As this aminotransferase can produce hydrogen sulfide and CdS nanoparticles if S2-is in the system, special measures must be taken.
BBa_K2242180 Reductase CmCR Coding 873 This reductase CmCR is a NADH-dependent reductase which can catalyze the bio-transformation from COBE to (S)-CHBE. Kindly provide by Prof.Hong Jiong As COBE is harmful to our skin and respiratory tract with pungent smell, special measures must be taken.
BBa_K2242626 Reductase KmAdh Coding 888 KmAdh is an alcohol dehydrogenase from Kluyveromyces marxianus. It can catalyze the ethanol fermentation ongoing in the bacteria with a high efficiency and mild reaction condition, especially temperature. Kindly provide by Prof.Hong Jiong
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