Difference between revisions of "Team:BIT-China/Parts"
| Line 13: | Line 13: | ||
</div> | </div> | ||
<div style="box-shadow: 0 0 0 transparent; margin-bottom: 100px;" class="my-banner" id="top"> | <div style="box-shadow: 0 0 0 transparent; margin-bottom: 100px;" class="my-banner" id="top"> | ||
| − | <img style="width: 90%" src="https://static.igem.org/mediawiki/2017/ | + | <img style="width: 90%" src="https://static.igem.org/mediawiki/2017/3/35/T--BIT-China--2017partspic2.jpg" alt=""> |
</div> | </div> | ||
<section class="content_container" id="mytop"> | <section class="content_container" id="mytop"> | ||
| Line 33: | Line 33: | ||
<tbody> | <tbody> | ||
<tr> | <tr> | ||
| − | <td colspan="4" class="my-colspan4"> | + | <td colspan="4" class="my-colspan4">Receptor expression</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 40: | Line 40: | ||
</td> | </td> | ||
<td>T1R2</td> | <td>T1R2</td> | ||
| − | <td>Human source | + | <td>Human source Sweet-receptor</td> |
| − | <td> | + | <td>2520</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 48: | Line 48: | ||
</td> | </td> | ||
<td>T1R3</td> | <td>T1R3</td> | ||
| − | <td>Human source | + | <td>Human source Sweet-receptor</td> |
| − | <td> | + | <td>1989</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 56: | Line 56: | ||
</td> | </td> | ||
<td>Gal 1/10-T1R2-CYC1</td> | <td>Gal 1/10-T1R2-CYC1</td> | ||
| − | <td>Completed expression of the human source | + | <td>Completed expression of the human source Sweet-receptor</td> |
| − | <td> | + | <td>3449</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 64: | Line 64: | ||
</td> | </td> | ||
<td>Gal 1/10-T1R3-ADH1</td> | <td>Gal 1/10-T1R3-ADH1</td> | ||
| − | <td>Completed expression of the human source | + | <td>Completed expression of the human source Sweet-receptor</td> |
| − | <td> | + | <td>2814</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 73: | Line 73: | ||
<td>MYC+T1R2 overlap</td> | <td>MYC+T1R2 overlap</td> | ||
<td>Marker genes of the Sweet-receptor</td> | <td>Marker genes of the Sweet-receptor</td> | ||
| − | <td></td> | + | <td>517</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 89: | Line 89: | ||
<td>HIS+T1R3 overlap</td> | <td>HIS+T1R3 overlap</td> | ||
<td>Marker genes of the Sweet-receptor</td> | <td>Marker genes of the Sweet-receptor</td> | ||
| − | <td></td> | + | <td>187</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 122: | Line 122: | ||
<td>BCP+T1R2 overlap</td> | <td>BCP+T1R2 overlap</td> | ||
<td>Marker genes of the Sweet-receptor</td> | <td>Marker genes of the Sweet-receptor</td> | ||
| − | <td> | + | <td>710</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 156: | Line 156: | ||
<td>500bp Homologous arm＋His</td> | <td>500bp Homologous arm＋His</td> | ||
<td>knocking out the sst2 gene</td> | <td>knocking out the sst2 gene</td> | ||
| − | <td> | + | <td>2050</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 164: | Line 164: | ||
<td>500bp Homologous arm＋Ura</td> | <td>500bp Homologous arm＋Ura</td> | ||
<td>knocking out the far1 gene</td> | <td>knocking out the far1 gene</td> | ||
| − | <td> | + | <td>2050</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 172: | Line 172: | ||
<td>500bp Homologous arm＋Tdp</td> | <td>500bp Homologous arm＋Tdp</td> | ||
<td>knocking out the ste2 gene</td> | <td>knocking out the ste2 gene</td> | ||
| − | <td> | + | <td>2071</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 180: | Line 180: | ||
<td>Modified Gα Schizosaccharomyces pombe</td> | <td>Modified Gα Schizosaccharomyces pombe</td> | ||
<td>Signal tdansduction</td> | <td>Signal tdansduction</td> | ||
| − | <td> | + | <td>1421</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 186: | Line 186: | ||
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368022">BBa_2368022</a> | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368022">BBa_2368022</a> | ||
</td> | </td> | ||
| − | <td>Modified Gα | + | <td>Modified Gα Caenorhabditis elegans</td> |
<td>Signal tdansduction</td> | <td>Signal tdansduction</td> | ||
| − | <td> | + | <td>1418</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 236: | Line 236: | ||
<td>Pfus+mRFP+CYC1T</td> | <td>Pfus+mRFP+CYC1T</td> | ||
<td>The reportor</td> | <td>The reportor</td> | ||
| − | <td> | + | <td>1375</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 244: | Line 244: | ||
<td>CYC1T A terminator in</td> | <td>CYC1T A terminator in</td> | ||
<td>the yeast</td> | <td>the yeast</td> | ||
| − | <td> | + | <td>231</td> |
| + | </tr> | ||
| + | <tr> | ||
| + | <td> | ||
| + | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368029">BBa_2368029</a> | ||
| + | </td> | ||
| + | <td> Pfus(2 STE12 binding sites)</td> | ||
| + | <td>Transformational promoter</td> | ||
| + | <td>213</td> | ||
</tr> | </tr> | ||
Revision as of 11:18, 26 October 2017
| Biobrick | Name | Description | Length |
|---|---|---|---|
| Receptor expression | |||
| BBa_2368003 | T1R2 | Human source Sweet-receptor | 2520 |
| BBa_2368004 | T1R3 | Human source Sweet-receptor | 1989 |
| BBa_2368007 | Gal 1/10-T1R2-CYC1 | Completed expression of the human source Sweet-receptor | 3449 |
| BBa_2368008 | Gal 1/10-T1R3-ADH1 | Completed expression of the human source Sweet-receptor | 2814 |
| BBa_2368023 | MYC+T1R2 overlap | Marker genes of the Sweet-receptor | 517 |
| BBa_2368009 | HIS+T1R2 overlap | Marker genes of the Sweet-receptor | 50 |
| BBa_2368024 | HIS+T1R3 overlap | Marker genes of the Sweet-receptor | 187 |
| BBa_2368011 | FLAG+T1R3 overlap | Marker genes of the Sweet-receptor | 50 |
| BBa_2368012 | YCP+T1R2 overlap | Marker genes of the Sweet-receptor | 740 |
| BBa_2368013 | YCP+T1R3 overlap | Marker genes of the Sweet-receptor | 740 |
| BBa_2368014 | BCP+T1R2 overlap | Marker genes of the Sweet-receptor | 710 |
| BBa_2368015 | GFP+T1R2 overlap | Marker genes of the Sweet-receptor | 699 |
| BBa_2368020 | BCP+T1R3 overlap | Marker genes of the Sweet-receptor | 710 |
| Host engineeringn | |||
| BBa_2368017 | 500bp Homologous arm＋His | knocking out the sst2 gene | 2050 |
| BBa_2368018 | 500bp Homologous arm＋Ura | knocking out the far1 gene | 2050 |
| BBa_2368016 | 500bp Homologous arm＋Tdp | knocking out the ste2 gene | 2071 |
| BBa_2368021 | Modified Gα Schizosaccharomyces pombe | Signal tdansduction | 1421 |
| BBa_2368022 | Modified Gα Caenorhabditis elegans | Signal tdansduction | 1418 |
| BBa_2368028 | Gα | Signal tdansduction | 1421 |
| Detect circuit | |||
| BBa_2368005 | Pfus | Induced promoter | 438 |
| BBa_2368026 | Pfus(4 STE12 binding sites) | Tdansformational promoter | 223 |
| BBa_2368027 | Pfus(6 STE12 binding sites) | Tdansformational promoter | 249 |
| BBa_2368006 | Pfus+mRFP+CYC1T | The reportor | 1375 |
| BBa_2368002 | CYC1T A terminator in | the yeast | 231 |
| BBa_2368029 | Pfus(2 STE12 binding sites) | Transformational promoter | 213 |
