Difference between revisions of "Team:UCC Ireland"
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<p id='home_page'> Our project aims to create a standardised universal biosensor strategy, that will be affordable and user friendly, containing all the components for readout. This system will consist of three elements: a bioengineered construct, the chassis and the readout device. | <p id='home_page'> Our project aims to create a standardised universal biosensor strategy, that will be affordable and user friendly, containing all the components for readout. This system will consist of three elements: a bioengineered construct, the chassis and the readout device. | ||
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1. Bioengineered Construct | 1. Bioengineered Construct | ||
Comprises a biological circuit designed to detect the presence of a specific substrate. This sensing element of each construct is specific to the substance being detected, while the readout from each construct is consistent = AmilCP (a blue coloured chromoprotein). | Comprises a biological circuit designed to detect the presence of a specific substrate. This sensing element of each construct is specific to the substance being detected, while the readout from each construct is consistent = AmilCP (a blue coloured chromoprotein). | ||
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2. Chassis | 2. Chassis | ||
The system is deployed initially through plasmid containing E. coli (tube-based), with the aim of progressing to a cell free system (as a lyophilised paper system). | The system is deployed initially through plasmid containing E. coli (tube-based), with the aim of progressing to a cell free system (as a lyophilised paper system). | ||
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3. Readout Device | 3. Readout Device | ||
The readout device has been 3D printed and is designed to attach to common smartphones. The device acts as a spectrophotometer, enabling easy measurement of the relative fluorescence or absorbance of a sample. Using a mobile app and algorithms developed by the team, the device compares the fluorescence/absorbance measurement to a standard curve and determine the concentration of the substance being tested for in the sample. | The readout device has been 3D printed and is designed to attach to common smartphones. The device acts as a spectrophotometer, enabling easy measurement of the relative fluorescence or absorbance of a sample. Using a mobile app and algorithms developed by the team, the device compares the fluorescence/absorbance measurement to a standard curve and determine the concentration of the substance being tested for in the sample. | ||
Revision as of 12:42, 28 October 2017
Our project aims to create a standardised universal biosensor strategy, that will be affordable and user friendly, containing all the components for readout. This system will consist of three elements: a bioengineered construct, the chassis and the readout device.
1. Bioengineered Construct
Comprises a biological circuit designed to detect the presence of a specific substrate. This sensing element of each construct is specific to the substance being detected, while the readout from each construct is consistent = AmilCP (a blue coloured chromoprotein).
2. Chassis The system is deployed initially through plasmid containing E. coli (tube-based), with the aim of progressing to a cell free system (as a lyophilised paper system).
3. Readout Device The readout device has been 3D printed and is designed to attach to common smartphones. The device acts as a spectrophotometer, enabling easy measurement of the relative fluorescence or absorbance of a sample. Using a mobile app and algorithms developed by the team, the device compares the fluorescence/absorbance measurement to a standard curve and determine the concentration of the substance being tested for in the sample. To validate the idea for our Universal Biosensor Strategy, we identified irish industries with a need for such a product. We have targeted the dairy and microbrewery industries as proof of concept for our project, to test for antibiotic or methanol residue.
