Difference between revisions of "Team:Sydney Australia/Parts"

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   <script src="http://code.jquery.com/jquery-2.1.0.min.js"></script>
 
   <script src="http://code.jquery.com/jquery-2.1.0.min.js"></script>
 
   <link href="https://fonts.googleapis.com/css?family=Quicksand" rel="stylesheet">
 
   <link href="https://fonts.googleapis.com/css?family=Quicksand" rel="stylesheet">
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  <link rel="stylesheet" href="https://maxcdn.bootstrapcdn.com/bootstrap/3.3.7/css/bootstrap.min.css">
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  <script src="https://maxcdn.bootstrapcdn.com/bootstrap/3.3.7/js/bootstrap.min.js"></script>
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<script src="http://code.jquery.com/jquery-2.1.0.min.js"></script>
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<link href="https://fonts.googleapis.com/css?family=Quicksand:300,400|Roboto:400,400i" rel="stylesheet">
 +
 
<style>  
 
<style>  
  
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#please h1 {
 
#please h1 {
 
font-family: "banaue", sans-serif;  
 
font-family: "banaue", sans-serif;  
−
color: #3e3f3f
+
color: #3e3f3f;
 +
font-size: 60px;
 +
}
 +
 
 +
#please h4 {
 +
  font-family: 'Quicksand', sans-serif;
 +
color: #3e3f3f;
 +
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 +
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 +
 
 
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}
  
 
#please p {
 
#please p {
 
   font-family: 'Quicksand', sans-serif;
 
   font-family: 'Quicksand', sans-serif;
−
color: #3e3f3f
+
color: #3e3f3f;
 +
 
 
}
 
}
  
−
.img-center  {
+
#please h3 {
−
image-rendering: -moz-crisp-edges;
+
  font-family: 'quicksand', sans-serif;
−
image-rendering: -o-crisp-edges;
+
color: #db4d4f;
−
image-rendering: -webkit-optimize-contrast;
+
 
−
-ms-interpolation-mode: nearest-neighbor;
+
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−
width:80%;
+
 
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+
#please h2 {
 +
  font-family: 'Quicksand', sans-serif;
 +
color: #a2cca3;
 +
font-size: 20.8px;
 +
 
 +
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 +
 
 +
#please h5 {
 +
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 +
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 +
font-size: 22px;
 +
font-weight: bold;
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 +
 
 +
#please h6 {
 +
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 +
color: #3e3f3f;
 +
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.crispy {
 
.crispy {
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-ms-interpolation-mode: nearest-neighbor;
 
-ms-interpolation-mode: nearest-neighbor;
 
   padding:0px;  
 
   padding:0px;  
−
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+
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   margin-top: 0px;  
 
   margin-top: 0px;  
−
   margin-left:0%;,  
+
   margin-left:5%;,  
−
   margin-right:0%;
+
   margin-right:5%;
−
background: #e2e2e2;
+
background: #fff;
 
}
 
}
  
−
table caption {
+
.img-center  {
−
padding: .5em 0;
+
image-rendering: -moz-crisp-edges;
−
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+
image-rendering: -o-crisp-edges;
−
 
+
image-rendering: -webkit-optimize-contrast;
−
table.dataTable th,
+
-ms-interpolation-mode: nearest-neighbor;
−
table.dataTable td {
+
width:80%;
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+
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+
  
 
.insert {
 
.insert {
 
   height: 68px;
 
   height: 68px;
 +
  width: 100%;
 +
  background-color:#fff;
 +
}
 +
.insert2 {
 +
  height: 150px;
 
   width: 100%
 
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−
   background-color:#e2e2e2;
+
   background-color:#fff;
 
}
 
}
 +
 +
.box {
 +
  background:#fff;
 +
  border:0px solid;
 +
  border-color: #e2e2e2;
 +
  margin-top: 0px;
 +
  box-sizing: border-box;
 +
  background-clip: padding-box;
 +
  padding:0 20px 20px 20px;
 +
  min-height:500px;
 +
  min-width:400px
 +
}
 +
 
.bannerbox {
 
.bannerbox {
 
   background:#e2e2e2;
 
   background:#e2e2e2;
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   height:750px;
 
   height:750px;
 
   width:100%;
 
   width:100%;
 +
}
 +
 +
.content {
 +
  padding:0px;
 +
  width:80%;
 +
  margin-top: 0px;
 +
  margin-left:10%;
 +
  margin-right:10%;
 +
}
 +
 +
 +
tr, tf {color:#3e3f3f;}
 +
tr td:hover { background: #e2e2e2; color: #3e3f3f; } 
 +
p, caption, h1 {font-family:'Quicksand';color:#3e3f3f;}
 +
 +
select {
 +
    width:30%;
 +
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 +
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 +
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 +
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 +
.box1, .box2, .box3, .box4, .box5 {
 +
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 +
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 +
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 +
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 +
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 +
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 +
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 +
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 +
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 +
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 +
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 +
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 +
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 +
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 +
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 +
 +
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 +
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 +
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 +
 +
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 +
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 +
  border-color: #93bcc1;
 
}
 
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</div>
 
</div>
  
−
<div class="container">
+
<div class="row" style="height:5vw;"></div>
−
  <div class="row" id = "please">
+
 
−
<div class="table-responsive">
+
<div class = "row">
−
<table summary="This table shows how to create responsive tables using Bootstrap's default functionality" class="table table-bordered table-hover">
+
<div class = "content" id="please">
−
        <thead>
+
<center><h1>GOLD</h1></center>
−
          <tr>
+
<div class="box1"></div>
−
            <th>Part Number</th>
+
<div class="row" style="height:2vw;"></div>
−
            <th>Part Name</th>
+
<table class="MsoTableGrid" border="1" cellspacing="0" cellpadding="0" width="99%" style="width:99.54%;border-collapse:collapse;border:none;mso-border-alt:solid windowtext .5pt;
−
            <th>Type</th>
+
mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt">
−
            <th>Function</th>
+
<tbody><tr style="mso-yfti-irow:0;mso-yfti-firstrow:yes;height:18.35pt">
−
            <th>Description</th>
+
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−
          </tr>
+
  mso-border-alt:solid windowtext .5pt;background:#E6BC2E;padding:0cm 5.4pt 0cm 5.4pt;
−
        </thead>
+
  height:18.35pt">
−
        <tbody>
+
  <p class="MsoNormal"><span lang="EN-US"><o:p>&nbsp;</o:p></span></p>
−
          <tr>
+
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:20.0pt;font-family:Quicksand">GOLD<o:p></o:p></span></p>
−
            <td>BBa_K2417000</td>
+
  <p class="MsoNormal"><span lang="EN-US"><o:p>&nbsp;</o:p></span></p>
−
            <td>Human Proinsulin</td>
+
  </td>
−
            <td>Basic</td>
+
</tr>
−
            <td>Hormone therapy substitute</td>
+
<tr style="mso-yfti-irow:1;height:19.05pt">
−
            <td>Bronze</td>
+
  <td width="19%" style="width:19.88%;border:solid windowtext 1.0pt;border-top:
−
          </tr>
+
  none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
−
    <tr>
+
  background:#F6D562;padding:0cm 5.4pt 0cm 5.4pt;height:19.05pt">
−
            <td>BBa_K2417001</td>
+
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
−
            <td>Winsulin</td>
+
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">PART NAME / NO.<o:p></o:p></span></p>
−
            <td>Basic</td>
+
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
−
            <td>Hormone therapy substitute</td>
+
  </td>
−
            <td>Bronze</td>
+
  <td width="14%" style="width:14.62%;border-top:none;border-left:none;
−
          </tr>
+
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
−
        </tbody>
+
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
−
        <tfoot>
+
  mso-border-alt:solid windowtext .5pt;background:#F6D562;padding:0cm 5.4pt 0cm 5.4pt;
−
      </table>
+
  height:19.05pt">
−
    </div>
+
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">TYPE<o:p></o:p></span></p>
−
   </div>
+
  </td>
 +
  <td width="21%" style="width:21.14%;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;background:#F6D562;padding:0cm 5.4pt 0cm 5.4pt;
 +
  height:19.05pt">
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">SOURCE<o:p></o:p></span></p>
 +
  </td>
 +
  <td width="44%" style="width:44.36%;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;background:#F6D562;padding:0cm 5.4pt 0cm 5.4pt;
 +
  height:19.05pt">
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">KEY FEATURES<o:p></o:p></span></p>
 +
  </td>
 +
</tr>
 +
<tr style="mso-yfti-irow:2;height:78.9pt">
 +
  <td width="19%" style="width:19.88%;border:solid windowtext 1.0pt;border-top:
 +
  none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 +
  padding:0cm 5.4pt 0cm 5.4pt;height:78.9pt">
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span class="SpellE"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">YncM-Winsulin</span></span><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p></o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">BBa_K2417005<o:p></o:p></span></p>
 +
  </td>
 +
  <td width="14%" style="width:14.62%;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:78.9pt">
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">COMPOSITE PART<o:p></o:p></span></p>
 +
  </td>
 +
  <td width="21%" style="width:21.14%;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:78.9pt">
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Bacillus subtilis (<span class="SpellE">YncM</span> secretion tag)<o:p></o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  </td>
 +
  <td width="44%" style="width:44.36%;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:78.9pt">
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoListParagraphCxSpFirst" style="text-indent:-18.0pt;mso-list:l1 level1 lfo3"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 +
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Novel short chain
 +
  insulin analogue (<span class="SpellE">Winsulin</span>) with a 12AA linker
 +
  (QRGGGSGGGQRR)<o:p></o:p></span></p>
 +
  <p class="MsoListParagraphCxSpMiddle" style="text-indent:-18.0pt;mso-list:l1 level1 lfo3"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 +
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span class="SpellE"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">YncM</span></span><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"> secretion tag to
 +
  translocate protein into media<o:p></o:p></span></p>
 +
  <p class="MsoListParagraphCxSpMiddle" style="text-indent:-18.0pt;mso-list:l1 level1 lfo3"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 +
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N-terminal 6x His
 +
  Tag purification<o:p></o:p></span></p>
 +
  <p class="MsoListParagraphCxSpMiddle" style="text-indent:-18.0pt;mso-list:l1 level1 lfo3"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 +
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">4x GSS flexible linker<o:p></o:p></span></p>
 +
  <p class="MsoListParagraphCxSpMiddle" style="text-indent:-18.0pt;mso-list:l1 level1 lfo3"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 +
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">TEV protease
 +
  cleavage site<o:p></o:p></span></p>
 +
  <p class="MsoListParagraphCxSpLast" style="text-indent:-18.0pt;mso-list:l1 level1 lfo3"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 +
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Extended ribosome
 +
  binding site<o:p></o:p></span></p>
 +
  <p class="MsoNormal" style="margin-left:18.0pt"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  </td>
 +
</tr>
 +
<tr style="mso-yfti-irow:3;height:78.9pt">
 +
  <td width="19%" rowspan="2" style="width:19.88%;border:solid windowtext 1.0pt;
 +
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 +
  padding:0cm 5.4pt 0cm 5.4pt;height:78.9pt">
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span class="SpellE"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Ecotin</span></span><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">-Proinsulin<o:p></o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">BBa_K2417000<o:p></o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  </td>
 +
  <td width="14%" rowspan="2" style="width:14.62%;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:78.9pt">
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">COMPOSITE PART<o:p></o:p></span></p>
 +
  </td>
 +
  <td width="21%" style="width:21.14%;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:78.9pt">
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">E. Coli (<span class="SpellE">Ecotin</span>
 +
  tag)<o:p></o:p></span></p>
 +
  </td>
 +
  <td width="44%" rowspan="2" style="width:44.36%;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
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 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:78.9pt">
 +
  <p class="MsoNormal" style="margin-left:18.0pt"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoListParagraphCxSpFirst" style="text-indent:-18.0pt;mso-list:l5 level1 lfo1"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 +
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Human proinsulin <o:p></o:p></span></p>
 +
  <p class="MsoListParagraphCxSpMiddle" style="text-indent:-18.0pt;mso-list:l5 level1 lfo1"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 +
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N-terminal <span class="SpellE">Ecotin</span> tag for targeting proteins to the periplasm<o:p></o:p></span></p>
 +
  <p class="MsoListParagraphCxSpMiddle" style="text-indent:-18.0pt;mso-list:l5 level1 lfo1"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 +
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N-terminal 6x His
 +
  tag <o:p></o:p></span></p>
 +
  <p class="MsoListParagraphCxSpMiddle" style="text-indent:-18.0pt;mso-list:l5 level1 lfo1"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 +
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">4x GGS flexible
 +
  linker<o:p></o:p></span></p>
 +
  <p class="MsoListParagraphCxSpMiddle" style="text-indent:-18.0pt;mso-list:l5 level1 lfo1"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 +
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">trypsin protease
 +
  cleavage site for His-tag and C-peptide cleavage<o:p></o:p></span></p>
 +
  <p class="MsoListParagraphCxSpMiddle" style="text-indent:-18.0pt;mso-list:l5 level1 lfo1"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 +
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Extended ribosomal
 +
  binding site <o:p></o:p></span></p>
 +
  <p class="MsoListParagraphCxSpLast"><span lang="EN-US" style="font-size:14.0pt;
 +
  font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  </td>
 +
</tr>
 +
<tr style="mso-yfti-irow:4;height:72.85pt">
 +
  <td width="21%" style="width:21.14%;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:72.85pt">
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Human (<span class="SpellE">Human</span>
 +
  proinsulin)<o:p></o:p></span></p>
 +
  </td>
 +
</tr>
 +
<tr style="mso-yfti-irow:5;mso-yfti-lastrow:yes;height:135.8pt">
 +
  <td width="19%" style="width:19.88%;border:solid windowtext 1.0pt;border-top:
 +
  none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 +
  padding:0cm 5.4pt 0cm 5.4pt;height:135.8pt">
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Cytoplasmic-Proinsulin <span style="mso-spacerun:yes">&nbsp;&nbsp;&nbsp;&nbsp;</span>BBa_K2417001<o:p></o:p></span></p>
 +
  </td>
 +
  <td width="14%" style="width:14.62%;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:135.8pt">
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">COMPOSITE PART<o:p></o:p></span></p>
 +
  </td>
 +
  <td width="21%" style="width:21.14%;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:135.8pt">
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Human (<span class="SpellE">Human</span>
 +
  proinsulin)<o:p></o:p></span></p>
 +
  </td>
 +
  <td width="44%" style="width:44.36%;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:135.8pt">
 +
  <p class="MsoListParagraphCxSpFirst" style="text-indent:-18.0pt;mso-list:l3 level1 lfo2"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 +
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Human proinsulin<o:p></o:p></span></p>
 +
  <p class="MsoListParagraphCxSpMiddle" style="text-indent:-18.0pt;mso-list:l3 level1 lfo2"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 +
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N-terminal 6x His
 +
  tag <o:p></o:p></span></p>
 +
  <p class="MsoListParagraphCxSpMiddle" style="text-indent:-18.0pt;mso-list:l3 level1 lfo2"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 +
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">4x GGS flexible linker<o:p></o:p></span></p>
 +
  <p class="MsoListParagraphCxSpMiddle" style="text-indent:-18.0pt;mso-list:l3 level1 lfo2"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 +
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Trypsin protease
 +
  cleavage site for His-tag and C-peptide cleavage<o:p></o:p></span></p>
 +
  <p class="MsoListParagraphCxSpLast" style="text-indent:-18.0pt;mso-list:l3 level1 lfo2"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 +
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Extended ribosomal
 +
  binding site <o:p></o:p></span></p>
 +
  </td>
 +
</tr>
 +
</tbody></table>
 +
</div>
 +
</div>
 +
 
 +
<div class="row" style="height:5vw;"></div>
 +
 
 +
<div class = "row">
 +
<div class = "content" id="please">
 +
<center><h1>SILVER</h1></center>
 +
<div class="box2"></div>
 +
<div class="row" style="height:2vw;"></div>
 +
<table class="MsoTableGrid" border="1" cellspacing="0" cellpadding="0" style="border-collapse:collapse;border:none;mso-border-alt:solid windowtext .5pt;
 +
mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt">
 +
<tbody><tr style="mso-yfti-irow:0;mso-yfti-firstrow:yes;height:18.3pt">
 +
  <td colspan="4" style="border:solid windowtext 1.0pt;mso-border-alt:solid windowtext .5pt;
 +
  background:#828282;padding:0cm 5.4pt 0cm 5.4pt;height:18.3pt">
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:20.0pt;font-family:Quicksand;color:white;mso-themecolor:
 +
  background1">SILVER<o:p></o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  </td>
 +
</tr>
 +
<tr style="mso-yfti-irow:1;height:18.3pt">
 +
  <td width="149" style="width:148.6pt;border:solid windowtext 1.0pt;border-top:
 +
  none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 +
  background:#CFCFCF;padding:0cm 5.4pt 0cm 5.4pt;height:18.3pt">
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">PART NAME <span class="GramE">/ <span style="mso-spacerun:yes">&nbsp;</span>NO.</span><o:p></o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  </td>
 +
  <td width="106" style="width:106.3pt;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;background:#CFCFCF;padding:0cm 5.4pt 0cm 5.4pt;
 +
  height:18.3pt">
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">TYPE<o:p></o:p></span></p>
 +
  </td>
 +
  <td width="108" style="width:108.3pt;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;background:#CFCFCF;padding:0cm 5.4pt 0cm 5.4pt;
 +
  height:18.3pt">
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">SOURCE<o:p></o:p></span></p>
 +
  </td>
 +
  <td width="334" style="width:334.3pt;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;background:#CFCFCF;padding:0cm 5.4pt 0cm 5.4pt;
 +
  height:18.3pt">
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">KEY FEATURES<o:p></o:p></span></p>
 +
  </td>
 +
</tr>
 +
<tr style="mso-yfti-irow:2;height:11.35pt">
 +
  <td width="149" style="width:148.6pt;border:solid windowtext 1.0pt;border-top:
 +
  none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 +
  padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt">
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Cytoplasmic-<span class="SpellE">Winsulin</span> <o:p></o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">BBa_K2417003<o:p></o:p></span></p>
 +
  </td>
 +
  <td width="106" style="width:106.3pt;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt">
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">COMPOSITE PART<o:p></o:p></span></p>
 +
  </td>
 +
  <td width="108" style="width:108.3pt;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt">
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N/A </span><span lang="EN-US" style="font-size:14.0pt;font-family:Helvetica;mso-fareast-font-family:Helvetica;
 +
  mso-bidi-font-family:Helvetica">–</span><span lang="EN-US" style="font-size:
 +
  14.0pt;font-family:Quicksand"> <span class="SpellE">Winsulin</span> is <span class="SpellE">self designed</span> from literature and mammalian insulins<o:p></o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  </td>
 +
  <td width="334" style="width:334.3pt;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt">
 +
  <p class="MsoListParagraphCxSpFirst"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></b></p>
 +
  <p class="MsoListParagraphCxSpMiddle" style="text-indent:-18.0pt;mso-list:l0 level1 lfo5"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 +
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Novel single chain
 +
  insulin analogue (<span class="SpellE">Winsulin</span>) with a 12AA linker
 +
  (QRGGGSGGGQRR)<b style="mso-bidi-font-weight:normal"><o:p></o:p></b></span></p>
 +
  <p class="MsoListParagraphCxSpMiddle" style="text-indent:-18.0pt;mso-list:l0 level1 lfo5"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 +
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N-terminal 6x His
 +
  Tag<b style="mso-bidi-font-weight:normal"><o:p></o:p></b></span></p>
 +
  <p class="MsoListParagraphCxSpMiddle" style="text-indent:-18.0pt;mso-list:l0 level1 lfo5"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 +
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">4x GSS flexible
 +
  linker<b style="mso-bidi-font-weight:normal"><o:p></o:p></b></span></p>
 +
  <p class="MsoListParagraphCxSpMiddle" style="text-indent:-18.0pt;mso-list:l0 level1 lfo5"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 +
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">TEV protease
 +
  cleavage site<b style="mso-bidi-font-weight:normal"><o:p></o:p></b></span></p>
 +
  <p class="MsoListParagraphCxSpMiddle" style="text-indent:-18.0pt;mso-list:l0 level1 lfo5"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 +
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Extended ribosome
 +
  binding site<b style="mso-bidi-font-weight:normal"><o:p></o:p></b></span></p>
 +
  <p class="MsoListParagraphCxSpLast"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></b></p>
 +
  </td>
 +
</tr>
 +
<tr style="mso-yfti-irow:3;height:114.0pt">
 +
  <td width="149" rowspan="2" style="width:148.6pt;border:solid windowtext 1.0pt;
 +
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 +
  padding:0cm 5.4pt 0cm 5.4pt;height:114.0pt">
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span class="SpellE"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Ecotin</span></span><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">-Proinsulin<o:p></o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">BBa_k2417000<o:p></o:p></span></p>
 +
  </td>
 +
  <td width="106" rowspan="2" style="width:106.3pt;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:114.0pt">
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">COMPOSITE PART<o:p></o:p></span></p>
 +
  </td>
 +
  <td width="108" style="width:108.3pt;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:114.0pt">
 +
  <p class="MsoNormal" align="center" style="text-align:center"><i style="mso-bidi-font-style:normal"><span lang="EN-US" style="font-size:14.0pt;
 +
  font-family:Quicksand">E. Coli </span></i><span lang="EN-US" style="font-size:
 +
  14.0pt;font-family:Quicksand">(<span class="SpellE">Ecotin</span> Tag)<o:p></o:p></span></p>
 +
  </td>
 +
  <td width="334" rowspan="2" style="width:334.3pt;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:114.0pt">
 +
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  <p class="MsoListParagraphCxSpFirst" style="text-indent:-18.0pt;mso-list:l4 level1 lfo4"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 +
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Human proinsulin<o:p></o:p></span></p>
 +
  <p class="MsoListParagraphCxSpMiddle" style="text-indent:-18.0pt;mso-list:l5 level1 lfo1"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 +
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N-terminal <span class="SpellE">Ecotin</span> tag for targeting proteins to the periplasm<o:p></o:p></span></p>
 +
  <p class="MsoListParagraphCxSpMiddle" style="text-indent:-18.0pt;mso-list:l4 level1 lfo4"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 +
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N <span class="GramE">terminal <span style="mso-spacerun:yes">&nbsp;</span>x</span> His
 +
  Tag <o:p></o:p></span></p>
 +
  <p class="MsoListParagraphCxSpMiddle" style="text-indent:-18.0pt;mso-list:l4 level1 lfo4"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 +
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">4x GSS flexible
 +
  linker<o:p></o:p></span></p>
 +
  <p class="MsoListParagraphCxSpMiddle" style="text-indent:-18.0pt;mso-list:l4 level1 lfo4"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 +
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">TEV protease
 +
  cleavage site <b style="mso-bidi-font-weight:normal"><o:p></o:p></b></span></p>
 +
  <p class="MsoListParagraphCxSpMiddle" style="text-indent:-18.0pt;mso-list:l4 level1 lfo4"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 +
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Extended ribosomal
 +
  binding site<b style="mso-bidi-font-weight:normal"><o:p></o:p></b></span></p>
 +
  <p class="MsoListParagraphCxSpLast"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></b></p>
 +
  </td>
 +
</tr>
 +
<tr style="mso-yfti-irow:4;mso-yfti-lastrow:yes;height:114.0pt">
 +
  <td width="108" style="width:108.3pt;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:114.0pt">
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Human (<span class="SpellE">Human</span>
 +
  proinsulin)<o:p></o:p></span></p>
 +
  </td>
 +
</tr>
 +
</tbody></table>
 +
</div>
 +
</div>
 +
 
 +
<div class="row" style="height:5vw;"></div>
 +
 
 +
<div class = "row">
 +
<div class = "content" id="please">
 +
<center><h1>BRONZE</h1></center>
 +
<div class="box3"></div>
 +
<div class="row" style="height:2vw;"></div>
 +
<table class="MsoTableGrid" border="1" cellspacing="0" cellpadding="0" width="100%" style="width:100.0%;border-collapse:collapse;border:none;mso-border-alt:solid windowtext .5pt;
 +
mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt">
 +
<tbody><tr style="mso-yfti-irow:0;mso-yfti-firstrow:yes;height:4.75pt">
 +
   <td width="100%" colspan="4" style="width:100.0%;border:solid windowtext 1.0pt;
 +
  mso-border-alt:solid windowtext .5pt;background:#A36952;padding:0cm 5.4pt 0cm 5.4pt;
 +
  height:4.75pt">
 +
  <p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-size:14.0pt;
 +
  font-family:Quicksand"><o:p>&nbsp;</o:p></span></b></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:20.0pt;font-family:Quicksand;color:white;mso-themecolor:
 +
  background1">BRONZE<o:p></o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-size:14.0pt;
 +
  font-family:Quicksand"><o:p>&nbsp;</o:p></span></b></p>
 +
  </td>
 +
</tr>
 +
<tr style="mso-yfti-irow:1;height:4.75pt">
 +
  <td width="21%" style="width:21.3%;border:solid windowtext 1.0pt;border-top:
 +
  none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 +
  background:#CD8B65;padding:0cm 5.4pt 0cm 5.4pt;height:4.75pt">
 +
  <p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-size:14.0pt;
 +
  font-family:Quicksand"><o:p>&nbsp;</o:p></span></b></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-size:14.0pt;
 +
  font-family:Quicksand">PART NAME / NO.<o:p></o:p></span></b></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-size:14.0pt;
 +
  font-family:Quicksand"><o:p>&nbsp;</o:p></span></b></p>
 +
  </td>
 +
  <td width="15%" style="width:15.24%;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;background:#CD8B65;padding:0cm 5.4pt 0cm 5.4pt;
 +
  height:4.75pt">
 +
  <p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-size:14.0pt;
 +
  font-family:Quicksand"><o:p>&nbsp;</o:p></span></b></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-size:14.0pt;
 +
  font-family:Quicksand">TYPE<o:p></o:p></span></b></p>
 +
  </td>
 +
  <td width="15%" style="width:15.5%;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;background:#CD8B65;padding:0cm 5.4pt 0cm 5.4pt;
 +
  height:4.75pt">
 +
  <p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-size:14.0pt;
 +
  font-family:Quicksand"><o:p>&nbsp;</o:p></span></b></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-size:14.0pt;
 +
  font-family:Quicksand">SOURCE<o:p></o:p></span></b></p>
 +
  </td>
 +
  <td width="47%" style="width:47.96%;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;background:#CD8B65;padding:0cm 5.4pt 0cm 5.4pt;
 +
  height:4.75pt">
 +
  <p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-size:14.0pt;
 +
  font-family:Quicksand"><o:p>&nbsp;</o:p></span></b></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-size:14.0pt;
 +
  font-family:Quicksand">KEY FEATURES<o:p></o:p></span></b></p>
 +
  </td>
 +
</tr>
 +
<tr style="mso-yfti-irow:2;height:35.15pt">
 +
  <td width="21%" rowspan="2" style="width:21.3%;border:solid windowtext 1.0pt;
 +
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 +
  padding:0cm 5.4pt 0cm 5.4pt;height:35.15pt">
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span class="SpellE"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Ecotin-Winsulin</span></span><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p></o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">BBa_K2417002<o:p></o:p></span></p>
 +
  </td>
 +
  <td width="15%" rowspan="2" style="width:15.24%;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:35.15pt">
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">COMPOSITE PART<o:p></o:p></span></p>
 +
  </td>
 +
  <td width="15%" style="width:15.5%;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:35.15pt">
 +
  <p class="MsoNormal" align="center" style="text-align:center"><i style="mso-bidi-font-style:normal"><span lang="EN-US" style="font-size:14.0pt;
 +
  font-family:Quicksand"><o:p>&nbsp;</o:p></span></i></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><i style="mso-bidi-font-style:normal"><span lang="EN-US" style="font-size:14.0pt;
 +
  font-family:Quicksand">E. Coli </span></i><span lang="EN-US" style="font-size:
 +
  14.0pt;font-family:Quicksand">(<span class="SpellE">Ecotin</span> Tag)<o:p></o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-size:14.0pt;
 +
  font-family:Quicksand"><o:p>&nbsp;</o:p></span></b></p>
 +
  </td>
 +
  <td width="47%" rowspan="2" style="width:47.96%;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:35.15pt">
 +
  <p class="MsoListParagraphCxSpFirst" style="text-indent:-18.0pt;mso-list:l2 level1 lfo6"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 +
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Novel single chain
 +
  insulin analogue (<span class="SpellE">Winsulin</span>) with a 12AA linker
 +
  (QRGGGSGGGQRR)<b style="mso-bidi-font-weight:normal"><o:p></o:p></b></span></p>
 +
  <p class="MsoListParagraphCxSpMiddle" style="text-indent:-18.0pt;mso-list:l0 level1 lfo5"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 +
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N-terminal <span class="SpellE">Ecotin</span> tag for targeting proteins to the periplasm<o:p></o:p></span></p>
 +
  <p class="MsoListParagraphCxSpMiddle" style="text-indent:-18.0pt;mso-list:l0 level1 lfo5"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 +
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N-terminal 6x His
 +
  Tag<b style="mso-bidi-font-weight:normal"><o:p></o:p></b></span></p>
 +
  <p class="MsoListParagraphCxSpMiddle" style="text-indent:-18.0pt;mso-list:l0 level1 lfo5"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 +
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">4x GSS flexible
 +
  linker <b style="mso-bidi-font-weight:normal"><o:p></o:p></b></span></p>
 +
  <p class="MsoListParagraphCxSpMiddle" style="text-indent:-18.0pt;mso-list:l0 level1 lfo5"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 +
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">TEV protease
 +
  cleavage site<b style="mso-bidi-font-weight:normal"><o:p></o:p></b></span></p>
 +
  <p class="MsoListParagraphCxSpLast" style="text-indent:-18.0pt;mso-list:l0 level1 lfo5"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 +
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Extended ribosome
 +
  binding site<b style="mso-bidi-font-weight:normal"><o:p></o:p></b></span></p>
 +
  </td>
 +
</tr>
 +
<tr style="mso-yfti-irow:3;mso-yfti-lastrow:yes;height:18.0pt">
 +
  <td width="15%" style="width:15.5%;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:18.0pt">
 +
  <p class="MsoNormal" align="center" style="text-align:center"><i style="mso-bidi-font-style:normal"><span lang="EN-US" style="font-size:14.0pt;
 +
  font-family:Quicksand"><o:p>&nbsp;</o:p></span></i></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><i style="mso-bidi-font-style:normal"><span lang="EN-US" style="font-size:14.0pt;
 +
  font-family:Quicksand">N/A </span></i><i style="mso-bidi-font-style:normal"><span lang="EN-US" style="font-size:14.0pt;font-family:Helvetica;mso-fareast-font-family:
 +
  Helvetica;mso-bidi-font-family:Helvetica">–</span></i><i style="mso-bidi-font-style:
 +
  normal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"> </span></i><span class="SpellE"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Winsulin</span></span><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"> is <span class="SpellE">self designed</span> from literature and mammalian insulins<o:p></o:p></span></p>
 +
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 +
  </td>
 +
</tr>
 +
</tbody></table>
 +
</div>
 +
</div>
  
 
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<div class="row" style="height:10vw;">
 +
 
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Revision as of 12:57, 28 October 2017

GOLD

 

GOLD

 

 

PART NAME / NO.

 

TYPE

SOURCE

KEY FEATURES

YncM-Winsulin

BBa_K2417005

COMPOSITE PART

 

Bacillus subtilis (YncM secretion tag)

 

 

·      Novel short chain insulin analogue (Winsulin) with a 12AA linker (QRGGGSGGGQRR)

·      YncM secretion tag to translocate protein into media

·      N-terminal 6x His Tag purification

·      4x GSS flexible linker

·      TEV protease cleavage site

·      Extended ribosome binding site

 

Ecotin-Proinsulin

BBa_K2417000

 

COMPOSITE PART

E. Coli (Ecotin tag)

 

·      Human proinsulin

·      N-terminal Ecotin tag for targeting proteins to the periplasm

·      N-terminal 6x His tag

·      4x GGS flexible linker

·      trypsin protease cleavage site for His-tag and C-peptide cleavage

·      Extended ribosomal binding site

 

Human (Human proinsulin)

Cytoplasmic-Proinsulin     BBa_K2417001

COMPOSITE PART

Human (Human proinsulin)

·      Human proinsulin

·      N-terminal 6x His tag

·      4x GGS flexible linker

·      Trypsin protease cleavage site for His-tag and C-peptide cleavage

·      Extended ribosomal binding site

SILVER

 

SILVER

 

 

PART NAME /  NO.

 

TYPE

SOURCE

KEY FEATURES

Cytoplasmic-Winsulin

BBa_K2417003

COMPOSITE PART

N/A – Winsulin is self designed from literature and mammalian insulins

 

 

·      Novel single chain insulin analogue (Winsulin) with a 12AA linker (QRGGGSGGGQRR)

·      N-terminal 6x His Tag

·      4x GSS flexible linker

·      TEV protease cleavage site

·      Extended ribosome binding site

 

Ecotin-Proinsulin

BBa_k2417000

COMPOSITE PART

E. Coli (Ecotin Tag)

 

·      Human proinsulin

·      N-terminal Ecotin tag for targeting proteins to the periplasm

·      N terminal  x His Tag

·      4x GSS flexible linker

·      TEV protease cleavage site

·      Extended ribosomal binding site

 

Human (Human proinsulin)

BRONZE

 

BRONZE

 

 

PART NAME / NO.

 

 

TYPE

 

SOURCE

 

KEY FEATURES

Ecotin-Winsulin

BBa_K2417002

COMPOSITE PART

 

E. Coli (Ecotin Tag)

 

·      Novel single chain insulin analogue (Winsulin) with a 12AA linker (QRGGGSGGGQRR)

·      N-terminal Ecotin tag for targeting proteins to the periplasm

·      N-terminal 6x His Tag

·      4x GSS flexible linker

·      TEV protease cleavage site

·      Extended ribosome binding site

 

N/A – Winsulin is self designed from literature and mammalian insulins