Difference between revisions of "Team:Sydney Australia/Parts"

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Revision as of 13:52, 28 October 2017

GOLD

 

GOLD

 

 

PART NAME /  NO.

 

TYPE

SOURCE

KEY FEATURES

YncM-Winsulin

BBa_K2417005

COMPOSITE PART

 

Bacillus subtilis (YncM secretion tag)

 

 

Novel short chain insulin analogue (Winsulin) with a 12AA linker (QRGGGSGGGQRR)

YncM secretion tag to translocate protein into media

N-terminal 6x His Tag purification

4x GSS flexible linker

TEV protease cleavage site

Extended ribosome binding site

 

Ecotin-Proinsulin

BBa_K2417000

COMPOSITE PART

E. Coli (Ecotin Tag)

 

Human proinsulin

N-terminal Ecotin tag for targeting proteins to the periplasm

N terminal 6x His Tag

4x GSS flexible linker

TEV protease cleavage site

Extended ribosomal binding site

 

Human (Human proinsulin)

Cytoplasmic-Proinsulin     BBa_K2417001

COMPOSITE PART

Human (Human proinsulin)

Human proinsulin

N-terminal 6x His tag

4x GGS flexible linker

Trypsin protease cleavage site for His-tag and C-peptide cleavage

Extended ribosomal binding site

SILVER

 

PART NAME /  NO.

 

TYPE

SOURCE

KEY FEATURES

Cytoplasmic-Winsulin

BBa_K2417003

COMPOSITE PART

 

N/A Winsulin is self designed from literature and mammalian insulins

 

 

Novel single chain insulin analogue (Winsulin) with a 12AA linker (QRGGGSGGGQRR)

N-terminal 6x His Tag

4x GSS flexible linker

TEV protease cleavage site

Extended ribosome binding site

 

Ecotin-Proinsulin

BBa_K2417000

COMPOSITE PART

E. Coli (Ecotin Tag)

 

Human proinsulin

N-terminal Ecotin tag for targeting proteins to the periplasm

N terminal 6x His Tag

4x GSS flexible linker

TEV protease cleavage site

Extended ribosomal binding site

 

Human (Human proinsulin)

BRONZE

 

BRONZE

 

 

PART NAME / NO.

 

TYPE

SOURCE

KEY FEATURES

Ecotin-Winsulin

BBa_K2417002

COMPOSITE PART

 

E. Coli (Ecotin Tag)

 

Novel single chain insulin analogue (Winsulin) with a 12AA linker (QRGGGSGGGQRR)

N-terminal Ecotin tag for targeting proteins to the periplasm

N-terminal 6x His Tag

4x GSS flexible linker

TEV protease cleavage site

Extended ribosome binding site

 

N/A Winsulin is self designed from literature and mammalian insulins