Difference between revisions of "Team:GZHS-United/Demonstrate"

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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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<h1>Demonstrate</h1>
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<h1>Demonstrate</h1>
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<p>To demonstrate that our system can actually work in real world, we designed two toxicity test to evaluate the toxicity of our engineered bacterial.</p>
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<h3>Gold Medal Criterion #4</h3>
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<p>This figure indicates the difference of lethality among <em>Bt.i</em>, <em>Bs</em>, cry4Ba <em>E.coli</em>, BL21 E.coli and the negative control. Cry4Ba <em>E.coli</em> and <em>Bti</em> exbited significantly higher toxicity than others. Toxicity of cry4Ba is lower than that of <em>Bt.i</em> because <em>Bt.i</em> contains other toxic proteins from Cry family. However, cry4Ba produced by our engineered <em>E.coli</em> could be purified and is more useful for industrial use.</p>
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<img src="https://static.igem.org/mediawiki/2017/9/92/T--GZHS-United--demonstrate1.png" class="demonstrate_img">
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Teams that can show their system working under real world conditions are usually good at impressing the judges in iGEM. To achieve gold medal criterion #4, convince the judges that your project works. There are many ways in which your project working could be demonstrated, so there is more than one way to meet this requirement. This gold medal criterion was introduced in 2016, so check our what 2016 teams did to achieve a their gold medals!
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<p>Our toxicity test results were showing below. According to the data, higher concentration have higher toxicity. We mainly use KT_50 as the parameter to judge the toxicity of toxin. </p>
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<p>It's abnormal that the concentration of 4% have higher KT_50 than 5%,</p>
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Please see the <a href="https://2017.igem.org/Judging/Medals">2017 Medals Page</a> for more information.
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<img src="https://static.igem.org/mediawiki/2017/7/76/T--GZHS-United--demonstrate2.png" class="demonstrate_img">
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<p>but according to the error bar, this may be just caused by  random error.</p>
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<p>Apart from these two toxicity tests, we also did a mathematical modeling to demonstrate our system. The result of modeling is showing in the chapter “<a href="https://2017.igem.org/Team:GZHS-United/Model">modeling</a>”</p>
  
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<h4> What should we do for our demonstration?</h4>
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<h5> Standard teams </h5>
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If you have built a proof of concept system, you can demonstrate it working under real world conditions. If you have built a biological device that is intended to be a sensor, can you show it detecting whatever it is intended to sense. If it is intended to work in the field, you can show how this might work using a simulated version in the lab, or a simulation of your device in the field.<strong> Please note biological materials must not be taken out of the lab</strong>.
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<h5> Special track teams </h5>
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Special track teams can achieve this medal criterion by bringing their work to the Jamboree and showcasing it in the track event. Art & Design, Measurement, Hardware and Software tracks will all have showcase events at the Giant Jamboree.<strong> Please note biological materials must not be taken out of the lab</strong>.
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Latest revision as of 17:42, 28 October 2017

demonstrate

Demonstrate

To demonstrate that our system can actually work in real world, we designed two toxicity test to evaluate the toxicity of our engineered bacterial.

This figure indicates the difference of lethality among Bt.i, Bs, cry4Ba E.coli, BL21 E.coli and the negative control. Cry4Ba E.coli and Bti exbited significantly higher toxicity than others. Toxicity of cry4Ba is lower than that of Bt.i because Bt.i contains other toxic proteins from Cry family. However, cry4Ba produced by our engineered E.coli could be purified and is more useful for industrial use.

Our toxicity test results were showing below. According to the data, higher concentration have higher toxicity. We mainly use KT_50 as the parameter to judge the toxicity of toxin.

It's abnormal that the concentration of 4% have higher KT_50 than 5%,

but according to the error bar, this may be just caused by random error.

Apart from these two toxicity tests, we also did a mathematical modeling to demonstrate our system. The result of modeling is showing in the chapter “modeling”