Line 75: | Line 75: | ||
<h1>Summary</h1> | <h1>Summary</h1> | ||
<div class="text" style="margin-bottom:3%;"> | <div class="text" style="margin-bottom:3%;"> | ||
− | + | DNA digestion, or DNA fragmentation, is a basic protocol in synthetic biology. This is typically performed prior to analysis of the DNA sequence, or in order to perform further protocols. Restriction enzymes are mixed and then incubated with DNA in a buffer solution. This yields DNA fragments cleaved at specific sites according to the enzymes used. | |
</div> | </div> | ||
</div> | </div> | ||
Line 88: | Line 88: | ||
<div class="col-md-9"> | <div class="col-md-9"> | ||
<div class="text"> | <div class="text"> | ||
− | This microfluidic chip | + | This microfluidic chip design performs DNA digestion. The desired DNA segment, restriction enzymes, water and buffer solution are inputted and mixed. The resulting solution is then sealed in an incubation chamber on top of which a heating element is placed. After the designated incubation time has passed, the liquid can then be transferred from the chip using a pipett |
</div> | </div> | ||
</div> | </div> | ||
Line 113: | Line 113: | ||
</div> | </div> | ||
− | <div class="container"> | + | <div class="container" style="margin-bottom:5%;"> |
<div class="tab-content gallery text-center"> | <div class="tab-content gallery text-center"> | ||
<div class="tab-pane active" id="Design"> | <div class="tab-pane active" id="Design"> | ||
<div class="col-md-6 text-center"> | <div class="col-md-6 text-center"> | ||
− | <img class="pics" src="https://static.igem.org/mediawiki/2017/ | + | <img class="pics" src="https://static.igem.org/mediawiki/2017/2/28/MARS_Digestion_F.png" alt="Picture" style="margin-top:20px; padding-top:18px;"> |
<div class="row text-center"> | <div class="row text-center"> | ||
<button class="btn btn-info btn-round"><span style="font-size:17px;">Flow Layer</span></button> | <button class="btn btn-info btn-round"><span style="font-size:17px;">Flow Layer</span></button> | ||
Line 123: | Line 123: | ||
</div> | </div> | ||
<div class="col-md-6 text-center"> | <div class="col-md-6 text-center"> | ||
− | <img class="pics" src="https://static.igem.org/mediawiki/2017/ | + | <img class="pics" src="https://static.igem.org/mediawiki/2017/f/fa/MARS_Digestion_C.png" alt="Picture" style="margin-top:20px; padding-top:18px;"> |
<div class="row text-center"> | <div class="row text-center"> | ||
<button class="btn btn-danger btn-round"><span style="font-size:17px;">Control Layer</span></button> | <button class="btn btn-danger btn-round"><span style="font-size:17px;">Control Layer</span></button> | ||
Line 131: | Line 131: | ||
<div class="tab-pane" id="Mill"> | <div class="tab-pane" id="Mill"> | ||
<div class="col-md-6 text-center"> | <div class="col-md-6 text-center"> | ||
− | <img class="pics" src="https://static.igem.org/mediawiki/2017/ | + | <img class="pics" src="https://static.igem.org/mediawiki/2017/6/6b/MARS_Digestion_MF.png" alt="Picture" style="margin-top:20px; padding-top:18px;"> |
<div class="row"> | <div class="row"> | ||
<button class="btn btn-info btn-round"><span style="font-size:17px;">Flow Layer</span></button> | <button class="btn btn-info btn-round"><span style="font-size:17px;">Flow Layer</span></button> | ||
Line 137: | Line 137: | ||
</div> | </div> | ||
<div class="col-md-6 text-center"> | <div class="col-md-6 text-center"> | ||
− | <img class="pics" src="https://static.igem.org/mediawiki/2017/ | + | <img class="pics" src="https://static.igem.org/mediawiki/2017/2/25/MARS_Digestion_MC.png" alt="Picture" style="margin-top:20px; padding-top:18px;"> |
<div class="row text-center"> | <div class="row text-center"> | ||
<button class="btn btn-danger btn-round"><span style="font-size:17px;">Control Layer</span></button> | <button class="btn btn-danger btn-round"><span style="font-size:17px;">Control Layer</span></button> | ||
Line 145: | Line 145: | ||
</div> | </div> | ||
</div> | </div> | ||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
</div> | </div> | ||
</div> | </div> |
Revision as of 03:45, 29 October 2017
![](https://static.igem.org/mediawiki/2017/0/02/MARSbackground.png)
![](https://static.igem.org/mediawiki/2017/2/22/MARSLogo2.png)
![](https://static.igem.org/mediawiki/2017/7/7a/MARS_Digestion.png)
Summary
DNA digestion, or DNA fragmentation, is a basic protocol in synthetic biology. This is typically performed prior to analysis of the DNA sequence, or in order to perform further protocols. Restriction enzymes are mixed and then incubated with DNA in a buffer solution. This yields DNA fragments cleaved at specific sites according to the enzymes used.
This microfluidic chip design performs DNA digestion. The desired DNA segment, restriction enzymes, water and buffer solution are inputted and mixed. The resulting solution is then sealed in an incubation chamber on top of which a heating element is placed. After the designated incubation time has passed, the liquid can then be transferred from the chip using a pipett
![Picture](https://static.igem.org/mediawiki/2017/2/28/MARS_Digestion_F.png)
![Picture](https://static.igem.org/mediawiki/2017/f/fa/MARS_Digestion_C.png)
![Picture](https://static.igem.org/mediawiki/2017/6/6b/MARS_Digestion_MF.png)
![Picture](https://static.igem.org/mediawiki/2017/2/25/MARS_Digestion_MC.png)