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− | <title>Cell | + | <title>Cell Sorting</title> |
<meta content='width=device-width, initial-scale=1.0, maximum-scale=1.0, user-scalable=0' name='viewport' /> | <meta content='width=device-width, initial-scale=1.0, maximum-scale=1.0, user-scalable=0' name='viewport' /> | ||
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<img src="https://static.igem.org/mediawiki/2017/0/02/MARSbackground.png" id="BACKGROUND"> | <img src="https://static.igem.org/mediawiki/2017/0/02/MARSbackground.png" id="BACKGROUND"> | ||
<img src="https://static.igem.org/mediawiki/2017/2/22/MARSLogo2.png" id="MARS"> | <img src="https://static.igem.org/mediawiki/2017/2/22/MARSLogo2.png" id="MARS"> | ||
− | <img src="https://static.igem.org/mediawiki/2017/ | + | <img src="https://static.igem.org/mediawiki/2017/e/ee/MARS_CellSorting.png" id="TITLE"> |
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<div class="main main-raised" style="margin-bottom:5%;"> | <div class="main main-raised" style="margin-bottom:5%;"> | ||
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<h1>Summary</h1> | <h1>Summary</h1> | ||
<div class="text" style="margin-bottom:3%;"> | <div class="text" style="margin-bottom:3%;"> | ||
− | + | The ability to sort cells by type or physical properties is a valuable tool in many synthetic biology labs. Prior to analysis or in order to perform specialised protocols, creating homogenous cell suspensions from a mixture is necessary. In addition to sorting cells, the removal of cell fragments, activated magnetic particles or unwanted debris through sorting also makes up a key part of purification protocols. | |
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<div class="text"> | <div class="text"> | ||
− | This microfluidic chip is | + | This microfluidic chip design carries out cell sorting as a cell suspension is passed through it. Cells are sorted based on size and pushed to the periphery of the channel. These cells are then carried away from the main solution through the two periphery outputs, and the cell-free solution can be collected from the central output. |
</div> | </div> | ||
</div> | </div> |
Revision as of 03:55, 29 October 2017
![](https://static.igem.org/mediawiki/2017/0/02/MARSbackground.png)
![](https://static.igem.org/mediawiki/2017/2/22/MARSLogo2.png)
![](https://static.igem.org/mediawiki/2017/e/ee/MARS_CellSorting.png)
Summary
The ability to sort cells by type or physical properties is a valuable tool in many synthetic biology labs. Prior to analysis or in order to perform specialised protocols, creating homogenous cell suspensions from a mixture is necessary. In addition to sorting cells, the removal of cell fragments, activated magnetic particles or unwanted debris through sorting also makes up a key part of purification protocols.
This microfluidic chip design carries out cell sorting as a cell suspension is passed through it. Cells are sorted based on size and pushed to the periphery of the channel. These cells are then carried away from the main solution through the two periphery outputs, and the cell-free solution can be collected from the central output.
![Picture](https://static.igem.org/mediawiki/2017/b/bb/MARS_Lysis_F.png)
![Picture](https://static.igem.org/mediawiki/2017/0/00/MARS_Lysis_C.png)
![Picture](https://static.igem.org/mediawiki/2017/0/08/MARS_Lysis_M.png)
![Picture](https://static.igem.org/mediawiki/2017/0/00/MARS_Lysis_C.png)
Testing
This following video shows a test of the chip using colored water. This is to show help explain the functionality of the chip.
No biological material was inserted into this chip.