Difference between revisions of "Team:BostonU HW/Fluorescence"
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<img src="https://static.igem.org/mediawiki/2017/0/02/MARSbackground.png" id="BACKGROUND"> | <img src="https://static.igem.org/mediawiki/2017/0/02/MARSbackground.png" id="BACKGROUND"> | ||
<img src="https://static.igem.org/mediawiki/2017/2/22/MARSLogo2.png" id="MARS"> | <img src="https://static.igem.org/mediawiki/2017/2/22/MARSLogo2.png" id="MARS"> | ||
| − | <img src="https://static.igem.org/mediawiki/2017/ | + | <img src="https://static.igem.org/mediawiki/2017/f/f0/MARS_Fluorescence.png" id="TITLE"> |
</div> | </div> | ||
<div class="main main-raised" style="margin-bottom:5%;"> | <div class="main main-raised" style="margin-bottom:5%;"> | ||
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<h1>Summary</h1> | <h1>Summary</h1> | ||
<div class="text" style="margin-bottom:3%;"> | <div class="text" style="margin-bottom:3%;"> | ||
| − | + | Fluorescence is commonly used as a quantifiable marker in synthetic biology procedures. Following the completion of a protocol, for example an assay test, samples are tested in a plate reader and the results analysed. | |
</div> | </div> | ||
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<div class="col-md-9"> | <div class="col-md-9"> | ||
<div class="text"> | <div class="text"> | ||
| − | This microfluidic chip is designed to perform | + | This microfluidic chip is designed to perform a Cell-free Toehold test, however it can be adapted to perform alternative fluorescent tests as desired. The metering primitive dispenses accurate ratio volumes of various substances. These are pushed into the central well which matches the dimensions of a 96-well plate. The chip can then be read in a plate reader with the use of an adapted skirt. |
</div> | </div> | ||
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<div class="tab-pane active" id="Design"> | <div class="tab-pane active" id="Design"> | ||
<div class="col-md-6 text-center"> | <div class="col-md-6 text-center"> | ||
| − | <img class="pics" src="https://static.igem.org/mediawiki/2017/ | + | <img class="pics" src="https://static.igem.org/mediawiki/2017/c/c3/MARS_Fluorescence_F.png" alt="Picture" style="margin-top:20px; padding-top:18px;"> |
<div class="row text-center" style="margin-bottom:5%;"> | <div class="row text-center" style="margin-bottom:5%;"> | ||
<button class="btn btn-info btn-round" ><span style="font-size:17px;">Flow Layer</span></button> | <button class="btn btn-info btn-round" ><span style="font-size:17px;">Flow Layer</span></button> | ||
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| − | <img class="pics" src="https://static.igem.org/mediawiki/2017/ | + | <img class="pics" src="https://static.igem.org/mediawiki/2017/6/68/MARS_Fluorescence_C.png" alt="Picture" style="margin-top:20px; padding-top:18px;"> |
<div class="row text-center" style="margin-bottom:5%;"> | <div class="row text-center" style="margin-bottom:5%;"> | ||
<button class="btn btn-danger btn-round"><span style="font-size:17px;">Control Layer</span></button> | <button class="btn btn-danger btn-round"><span style="font-size:17px;">Control Layer</span></button> | ||
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<div class="tab-pane" id="Mill"> | <div class="tab-pane" id="Mill"> | ||
<div class="col-md-6 text-center"> | <div class="col-md-6 text-center"> | ||
| − | <img class="pics" src="https://static.igem.org/mediawiki/2017/ | + | <img class="pics" src="https://static.igem.org/mediawiki/2017/a/a4/MARS_Fluorescence_MF.png" alt="Picture" style="margin-top:20px; padding-top:18px;"> |
<div class="row"> | <div class="row"> | ||
<button class="btn btn-info btn-round" style="margin-bottom:5%;"><span style="font-size:17px;">Flow Layer</span></button> | <button class="btn btn-info btn-round" style="margin-bottom:5%;"><span style="font-size:17px;">Flow Layer</span></button> | ||
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<div class="col-md-6 text-center"> | <div class="col-md-6 text-center"> | ||
| − | <img class="pics" src="https://static.igem.org/mediawiki/2017/ | + | <img class="pics" src="https://static.igem.org/mediawiki/2017/3/3a/MARS_Fluorescence_MC.png" alt="Picture" style="margin-top:20px; padding-top:18px;"> |
<div class="row text-center"> | <div class="row text-center"> | ||
<button class="btn btn-danger btn-round" style="margin-bottom:5%;"><span style="font-size:17px;">Control Layer</span></button> | <button class="btn btn-danger btn-round" style="margin-bottom:5%;"><span style="font-size:17px;">Control Layer</span></button> | ||
Revision as of 06:18, 29 October 2017
Summary
Fluorescence is commonly used as a quantifiable marker in synthetic biology procedures. Following the completion of a protocol, for example an assay test, samples are tested in a plate reader and the results analysed.
This microfluidic chip is designed to perform a Cell-free Toehold test, however it can be adapted to perform alternative fluorescent tests as desired. The metering primitive dispenses accurate ratio volumes of various substances. These are pushed into the central well which matches the dimensions of a 96-well plate. The chip can then be read in a plate reader with the use of an adapted skirt.
