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− | <b>Establishment of two orthogonal methods for the detection of unnatural base pairs in a target sequence via Oxford Nanopore sequencing and an enzyme based detection method</b> | + | <b>Establishment of two orthogonal methods for the detection of unnatural base pairs in a target sequence via <a href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Software">Oxford Nanopore sequencing</a> and an enzyme based detection method</b> |
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− | <b>Development of a software suite for these orthogonal methods </b> | + | <b>Development of a <a href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Software">software</a> suite for these orthogonal methods </b> |
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− | <b>Integration and characterization of the nucleotide transporter PtNTT2 from <i>P.tricornutum</i> in <i>E.coli</i> for the uptake of unnatural nucleoside triphosphates</b> | + | <b>Integration and characterization of the <a href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Results/unnatural_base_pair/uptake_and_biosynthesis">nucleotide transporter PtNTT2</a> from <i>P.tricornutum</i> in <i>E.coli</i> for the uptake of unnatural nucleoside triphosphates</b> |
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− | <b>Proof that certain Taq-polymerases can efficiently incorporate unnatural nucleotides </b> | + | <b>Proof that certain Taq-polymerases can efficiently <a href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Results/unnatural_base_pair/preservation_system">incorporate unnatural nucleotides</a> </b> |
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− | <b>Construction of a toolkit consisting of five aminoacyl-tRNA synthetases for incorporation of non-canonical amino acids</b> | + | <b>Construction of a <a href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Results/toolbox">toolkit</a> consisting of five aminoacyl-tRNA synthetases for incorporation of non-canonical amino acids</b> |
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− | <b>Development of a photoswitchable lycopene pathway</b> | + | <b>Development of a <a href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Results/toolbox/photoswitching">photoswitchable lycopene pathway</a></b> |
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− | <b>Design and chemical synthesis of a novel, fully synthetic amino acid based on cyanonitrobenzothiazol and asparagine and proof of its functionality</b> | + | <b>Design and <a href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Results/toolbox/fusing">chemical synthesis</a> of a novel, fully synthetic amino acid based on cyanonitrobenzothiazol and asparagine and proof of its functionality</b> |
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Revision as of 10:47, 29 October 2017
![](https://static.igem.org/mediawiki/2017/a/ae/T--Bielefeld-CeBiTec--title-img-cebitec.jpg)
iGEM Bielefeld-CeBiTec 2017
![](https://static.igem.org/mediawiki/2017/thumb/6/6b/T--Bielefeld-CeBiTec--SVI-Teamfoto-Labor.png/1200px-T--Bielefeld-CeBiTec--SVI-Teamfoto-Labor.png.jpeg)
Expanding the Genetic Code
For more information visit our project poster on reasearch gate
Achievements
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Establishment of two orthogonal methods for the detection of unnatural base pairs in a target sequence via Oxford Nanopore sequencing and an enzyme based detection method
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Development of a software suite for these orthogonal methods
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Integration and characterization of the nucleotide transporter PtNTT2 from P.tricornutum in E.coli for the uptake of unnatural nucleoside triphosphates
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Proof that certain Taq-polymerases can efficiently incorporate unnatural nucleotides
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Construction of a toolkit consisting of five aminoacyl-tRNA synthetases for incorporation of non-canonical amino acids
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Development of a photoswitchable lycopene pathway
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Design and chemical synthesis of a novel, fully synthetic amino acid based on cyanonitrobenzothiazol and asparagine and proof of its functionality
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Modeling more than ten new aaRS sequences
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Library development with several thousand sequences for selecting aminoacyl-tRNA synthetases
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Construction of positive and negative selection plasmids for the evolution of new synthetases for non-canonical amino acids
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Improvement of an aminoacyl-tRNA synthetase test-system by introducing a FRET-system and development of a ranking system
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Construction of an LED panel for irradiating 96-well microtiter plates, which can be used to manipulate non-canonical amino acids and much more
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Development of an Android App to control the LED panel with your smartphone via Bluetooth
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Writing of a biosafety report titled “Auxotrophy to Xeno-DNA: A Comprehensive Exploration of Combinatorial Mechanisms for a High-Fidelity Biosafety System”
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Writing of the ChImp Report on the “Chances and Implications of an Expanded Genetic Code”