Difference between revisions of "Team:USTC/Experiments"

 
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<title>子网页测试-队员</title>
 
<title>子网页测试-队员</title>
 
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                   <li class="bg_all"><a href="https://2017.igem.org/Team:USTC/Description">Description</a></li>
 
                   <li class="bg_all"><a href="https://2017.igem.org/Team:USTC/Description">Description</a></li>
 
                   <li class="bg_all"><a href="https://2017.igem.org/Team:USTC/Design">Design</a></li>
 
                   <li class="bg_all"><a href="https://2017.igem.org/Team:USTC/Design">Design</a></li>
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                   <li class="bg_all"><a href="https://2017.igem.org/Team:USTC/Results">Results</a></li>
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                   <li class="bg_all"><a href="https://2017.igem.org/Team:USTC/Demonstrate">Results</a></li>
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                   <li class="bg_all"><a href="https://2017.igem.org/Team:USTC/Demonstrate">Demonstrate</a></li>
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                   <li><a href="https://2017.igem.org/Team:USTC/Demonstrate/1">&nbsp;&nbsp;>Conduction</a></li>
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                  <li><a href="https://2017.igem.org/Team:USTC/Demonstrate/2">&nbsp;&nbsp;>Photocatalyst</a></li>
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                  <li><a href="https://2017.igem.org/Team:USTC/Demonstrate/3">&nbsp;&nbsp;>Harvest</a></li>
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                 </ul>
 
                 </ul>
 
               </div>
 
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<a href="https://2017.igem.org/Team:USTC/Safety" class="waves-effect waves-teal">Safety</a>
 
<a href="https://2017.igem.org/Team:USTC/Safety" class="waves-effect waves-teal">Safety</a>
 
</li>
 
</li>
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<li class="bold1 mintcream_choosed">
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<li class="bold honeydew_choosed"><a class="collapsible-header  waves-effect waves-teal">Model</a>
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<a href="https://2017.igem.org/Team:USTC/Model" class="waves-effect waves-teal">Model</a>
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              <div class="collapsible-body">
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</li>
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                <ul>
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                  <li class="bg_all"><a href="https://2017.igem.org/Team:USTC/Model">Overview</a></li>
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                  <li class="bg_all"><a href="https://2017.igem.org/Team:USTC/Model/2">DLA Crystal</a></li>
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                  <li id="static_word" class="bg_all"><a>Electron transfer</a></li>
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                  <li><a href="https://2017.igem.org/Team:USTC/Model/4">&nbsp;&nbsp;>Semi-conductor</a></li>
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                  <li><a href="https://2017.igem.org/Team:USTC/Model/3">&nbsp;&nbsp;>Markov</a></li>
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                  <li><a href="https://2017.igem.org/Team:USTC/Model/1">&nbsp;&nbsp;>MeCiM</a></li>
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                  <li class="bg_all"><a href="https://2017.igem.org/Team:USTC/Model/5">UPEP</a></li>
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                </ul>
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              </div>
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            </li>
  
 
             <li class="bold honeydew_choosed"><a class="collapsible-header  waves-effect waves-teal">Parts</a>
 
             <li class="bold honeydew_choosed"><a class="collapsible-header  waves-effect waves-teal">Parts</a>
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<div >
 
<div >
 
                                                         <br>
 
                                                         <br>
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        <p id="first" class="scrollspy label label-pink">Protocols</p>
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        <p id="first" class="scrollspy label label-pink">Protocols</p><br><br>
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        <a href="https://static.igem.org/mediawiki/2017/f/f9/USTC-note-pro.pdf" class="indent_word">Click here to check out the protocols of our experiments!</a>
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                                                        <br><br><br>
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</div>
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<div >
 
                                                         <br>
 
                                                         <br>
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                                                        <p>Plasmid Extraction</p>
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        <p id="second" class="scrollspy label label-pink">Materials</p><br><br>
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                                                        <p>Materials
:</p>
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        <a href="https://static.igem.org/mediawiki/2017/d/dd/USTC-note-mat.pdf" class="indent_word">Click here to check out the preparation for different materials we have used!</a>
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                                                        <p>Buffer P1 (stored in 4 degree)
</p>
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                                                         <br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br>
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                                                        <p>Buffer P2
</p>
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                                                        <p>Buffer p3
</p>
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                                                        <p>VisualLyse</p>
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                                                        <p>
Buffer DW1
</p>
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                                                        <p>Wash Solution
dd </p>
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                                                         <p>ddH<sub>2</sub>O</p>
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                                                        <p>
Prepation
</p>
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                                                        <p>1.Check out whether RNaseA has been added in Buffer P1.

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                                                        <p>2.Check out whether ethyl alcohol has been added in Wash Solution

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                                                        <p>3.Check out whether sediment exist in Buffer P2 and P2.
Procedure

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                                                        <p>4.Absorb 1.5 to 5 mL bacteria solution in to EP tubes, centrifuge them at 8,000xg 2 mins and then discard the culture medium.

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                                                        <p>5.Add 250 ul Buffer P1 into sediment, and use spearhead to make bacteria suspended.

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                                                        <p>6.Add 250 ul Buffer P2, and overturn the EP tubes 5 to 10 times immediately and tenderly. Stand tubes about 2 to 4 mins.

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                                                        <p>7.Add 350 ul Buffer P3 and overturn the EP tubes 5 to 10 times again immediately and tenderly.

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                                                        <p>8.Centrifuge tubes at 12,000xg about 5 to 10 mins.

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                                                        <p>9.Pour the supernatant liquid into absorption columns ,centrifuge them at 8,000xg about 30 sec and then discard the liquid in the collecting pipe.

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                                                        <p>10.optional:Add 500 ul Buffer DW1 and centrifuge them at 9,000xg about 30 sec. Then discard the liquid in the collecting pipe.

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                                                        <p>11.Add 500 ul Wash Solution, centrifuge them at 9,000xg about 30 sec and discard the liquid in the collecting pipe.
12.Do the step 11 again.
13.Centrifuge the empty columns at 9,000xg about 1 min.
14.Put the columns in clean 1.5mL EP tubes, add 50 to 100 ul Elution Buffer on the absorption film, stand 10 min and centrifuge about 1 min at 9,000xg.
15.Keep the DNA solution for further work.
The Protocol is based on SanPrep Column Type DNA Plasmid Extraction Kit.</p>
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  <span id="second" class="scrollspy label label-pink">Materials</span>
 
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<img src="https://static.igem.org/mediawiki/2017/4/49/Ustc_USTCIF.jpg" width="8%" height="8%">
 
<img src="https://static.igem.org/mediawiki/2017/4/49/Ustc_USTCIF.jpg" width="8%" height="8%">
 
<img src="https://static.igem.org/mediawiki/2017/4/4f/Ustc_ustc_teach.png" width="45%" height="78%">
 
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Latest revision as of 15:21, 29 October 2017

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