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<p><img src="https://static.igem.org/mediawiki/2017/1/13/T--TU_Darmstadt--Synthase_Pikto_fertig-neu.png" style="float:left;margin-right:1%;width:15%"> | <p><img src="https://static.igem.org/mediawiki/2017/1/13/T--TU_Darmstadt--Synthase_Pikto_fertig-neu.png" style="float:left;margin-right:1%;width:15%"> | ||
The functionality of the NodC enzyme was verified by performing the <i>UDP-Glo<sup>TM</sup> Glycosyltransferase</i> Assay. The evaluation of the assay shows that the NodC enzyme converts the UDP-GlcNAc to free UPD and a growing oligo-GlcNAc-chain. The free UDP is converted to ATP, which acts as a substrate for a luciferase reaction and creates luminescence. So the assay and the increasing luminescence depending on increasing enzyme concentrations shows that the NodC enzyme can create chitin oligomers. | The functionality of the NodC enzyme was verified by performing the <i>UDP-Glo<sup>TM</sup> Glycosyltransferase</i> Assay. The evaluation of the assay shows that the NodC enzyme converts the UDP-GlcNAc to free UPD and a growing oligo-GlcNAc-chain. The free UDP is converted to ATP, which acts as a substrate for a luciferase reaction and creates luminescence. So the assay and the increasing luminescence depending on increasing enzyme concentrations shows that the NodC enzyme can create chitin oligomers. | ||
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<center><img src="https://static.igem.org/mediawiki/2017/6/69/T--TU_Darmstadt--40ng_mit_allem.png" width="80%"></center> | <center><img src="https://static.igem.org/mediawiki/2017/6/69/T--TU_Darmstadt--40ng_mit_allem.png" width="80%"></center> | ||
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Revision as of 21:02, 29 October 2017
ChiTUcare
Proof of Concept
Here we want to give you a short tour about what we achieved in the project. We show our results and give a view on the resulting product, a enzyme sensing hydrogel.
Chitin Synthase NodC
The functionality of the NodC enzyme was verified by performing the UDP-GloTM Glycosyltransferase Assay. The evaluation of the assay shows that the NodC enzyme converts the UDP-GlcNAc to free UPD and a growing oligo-GlcNAc-chain. The free UDP is converted to ATP, which acts as a substrate for a luciferase reaction and creates luminescence. So the assay and the increasing luminescence depending on increasing enzyme concentrations shows that the NodC enzyme can create chitin oligomers.