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| | + | {{Template:Home}} |
| | <html> | | <html> |
| − |
| |
| − | <!--- THIS IS WHERE THE HTML BEGINS --->
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| − |
| |
| − | <head>
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| − | <link href="https://netdna.bootstrapcdn.com/bootstrap/3.0.0/css/bootstrap.min.css" rel="stylesheet"
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| − | id="bootstrap-css">
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| − | <style>
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| − |
| |
| − |
| |
| − | /***************************************************** DEFAULT WIKI SETTINGS ****************************************************/
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| − |
| |
| − | /* Clear the default wiki settings */
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| − |
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| − | #home_logo, #sideMenu {
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| − | display: none;
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| − | }
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| − |
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| − | #sideMenu, #top_title, .patrollink {
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| − | display: none;
| |
| − | }
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| − |
| |
| − | #content {
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| − | width: 100%;
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| − | padding: 0px;
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| − | margin-top: -7px;
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| − | margin-left: 0px;
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| − | }
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| − |
| |
| − | body {
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| − | background: white;
| |
| − | }
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| − |
| |
| − | #bodyContent h1, #bodyContent h2, #bodyContent h3, #bodyContent h4, #bodyContent h5 {
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| − | margin-bottom: 0;
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| − | color: #F8CE63;
| |
| − | }
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| − |
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| − | table {;
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| − | }
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| − |
| |
| − | /**************************************************************** MENU ***************************************************************/
| |
| − | /* Wrapper for the menu */
| |
| − | .igem_2017_menu_wrapper {
| |
| − | width: 15%;
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| − | height: 100vh;
| |
| − | position: fixed;
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| − | right: 0;
| |
| − | padding: 0;
| |
| − | float: right;
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| − | border-left: 1px solid #F8CE63;
| |
| − | background-color: white;
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| − | text-align: left;
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| − | font-family: Tahoma, Geneva, sans-serif;
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| − | overflow-y: auto;
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| − | overflow-x: hidden;
| |
| − | }
| |
| − |
| |
| − | /* this hides the scrollbar to keep view consistency */
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| − | .igem_2017_menu_wrappe::-webkit-scrollbar {
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| − | display: none;
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| − | }
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| − |
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| − | /* styling for links in the menu, removes the line under text */
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| − | .igem_2017_menu_wrapper a {
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| − | text-decoration: none;
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| − | }
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| − |
| |
| − | /* styling for the images in the menu */
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| − | .igem_2017_menu_wrapper img {
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| − | width: 100%;
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| − | }
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| − |
| |
| − | /* styling for the menu buttons */
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| − | .igem_2017_menu_wrapper .menu_button {
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| − | width: 100%;
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| − | padding: 10px 0px 10px 15px;
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| − | float: left;
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| − | border-bottom: 1px solid #F8CE63;
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| − | font-size: 16px;
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| − | font-weight: bold;
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| − | color: #F8CE63;
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| − | cursor: pointer;
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| − | }
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| − |
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| − | .igem_2017_menu_wrapper .menu_bottom_padding {
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| − | width: 100%;
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| − | height: 30px;
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| − | float: left;
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| − | }
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| − |
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| − | .menu_button.direct_to_page {
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| − | padding-left: 36px;
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| − | }
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| − |
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| − | .igem_2017_menu_wrapper .menu_button .expand_collapse_icon {
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| − | width: 10%;
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| − | float: left;
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| − | }
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| − |
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| − | .igem_2017_menu_wrapper .menu_button .expand_collapse_icon::before {
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| − | content: "+";
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| − | }
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| − |
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| − | /* styling for the menu buttons on hover */
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| − | .igem_2017_menu_wrapper .menu_button:hover, .igem_2017_menu_wrapper .submenu_button:hover, .submenu_button.current_page:hover {
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| − | background-color: #F8CE63;
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| − | text-decoration: none;
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| − | color: #190707;
| |
| − | }
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| − |
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| − | /* styling for the menu button when it is the current page */
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| − | .current_page {
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| − | background-color: white !important;
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| − | color: #F8CE63 !important;
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| − | }
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| − |
| |
| − | /* styling for the submenu buttons */
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| − | .igem_2017_menu_wrapper .submenu_button {
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| − | width: 100%;
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| − | padding: 10px 0px 10px 34px;
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| − | float: left;
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| − | background-color: white;
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| − | border-bottom: 1px solid #F8CE63;
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| − | font-size: 15px;
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| − | color: #F8CE63;
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| − | cursor: pointer;
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| − | }
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| − |
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| − | /* wrapper for the submenu items, they are hidden by default*/
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| − | .igem_2017_menu_wrapper .submenu_wrapper {
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| − | display: none;
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| − | }
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| − |
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| − | /* when the page size is bigger than 800px, this show/hide control is hidden by default */
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| − | .igem_2017_menu_wrapper #display_menu_control {
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| − | display: none;
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| − | text-align: center;
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| − | }
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| − |
| |
| − | /***************************************************** CONTENT OF THE PAGE ****************************************************/
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| − |
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| − | /* Wrapper for the content */
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| − | .igem_2017_content_wrapper {
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| − | width: 83%;
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| − | margin: 0;
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| − | display: block;
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| − | float: left;
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| − | background-color: white;
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| − | font-family: Tahoma, Geneva, sans-serif;
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| − | }
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| − |
| |
| − | /********************************* HTML STYLING *********************************/
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| − |
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| − | /* styling for the titles h1 h2 */
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| − | .igem_2017_content_wrapper h1, .igem_2017_content_wrapper h2 {
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| − | color: #F8CE63;
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| − | padding: 15px 0 25px 0;
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| − | border-bottom: 0;
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| − | }
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| − |
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| − | /* styling for the titles h3 h4 h5 h6*/
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| − | .igem_2017_content_wrapper h3, .igem_2017_content_wrapper h4, .igem_2017_content_wrapper h5, .igem_2017_content_wrapper h6 {
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| − | padding: 5px 15px;
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| − | border-bottom: 0;
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| − | }
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| − |
| |
| − | /* font and text */
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| − | .container p,
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| − | #HQ_page p {
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| − | line-height: 24px;
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| − | font-size: 16px;
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| − | color: #333;
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| − | }
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| − |
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| − | .igem_2017_content_wrapper img {
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| − | padding: 15px 0;
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| − | }
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| − |
| |
| − | /* Links */
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| − | .igem_2017_content_wrapper a {
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| − | font-weight: bold;
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| − | text-decoration: underline;
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| − | text-decoration-color: #190707;
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| − | color: #3399ff;
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| − | -webkit-transition: all 0.4s ease;
| |
| − | -moz-transition: all 0.4s ease;
| |
| − | -ms-transition: all 0.4s ease;
| |
| − | -o-transition: all 0.4s ease;
| |
| − | transition: all 0.4s ease;
| |
| − | }
| |
| − |
| |
| − | /* hover for the links */
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| − | .igem_2017_content_wrapper a:hover {
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| − | text-decoration: none;
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| − | color: #000000;
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| − | }
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| − |
| |
| − | /* non numbered lists */
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| − | .igem_2017_content_wrapper ul {
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| − | padding: 0px 20px;
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| − | font-size: 15px;
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| − | font-family: Tahoma, Geneva, sans-serif;
| |
| − | }
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| − |
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| − | /* numbered lists */
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| − | .igem_2017_content_wrapper ol {
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| − | padding: 0px;
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| − | font-size: 15px;
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| − | font-family: Tahoma, Geneva, sans-serif;
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| − | }
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| − |
| |
| − | /* Table */
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| − | .igem_2017_content_wrapper table {
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| − | width: 97%;
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| − | margin: 15px 10px;
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| − | border: 2px solid #190707;
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| − | border-collapse: collapse;
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| − | }
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| − |
| |
| − | /* table cells */
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| − | .igem_2017_content_wrapper td {
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| − | padding: 10px;
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| − | vertical-align: text-top;
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| − | border: 1px solid #190707;
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| − | border-collapse: collapse;
| |
| − | }
| |
| − |
| |
| − | /* table headers */
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| − | .igem_2017_content_wrapper th {
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| − | padding: 10px;
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| − | vertical-align: text-top;
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| − | border: 1px solid #190707;
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| − | border-collapse: collapse;
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| − |
| |
| − | }
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| − |
| |
| − | /**********************************LAYOUT CLASSES **********************************/
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| − |
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| − | /* general class for column divs */
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| − | .igem_2017_content_wrapper .column {
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| − | padding: 10px 0px;
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| − | float: left;
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| − | }
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| − |
| |
| − | /* class for a full width column */
| |
| − | .column .full_size {
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| − | width: 100%;
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| − | }
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| − |
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| − | /* styling for images in a full width column*/
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| − | .column.full_size img {
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| − | width: 97%;
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| − | padding: 10px 15px;
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| − | }
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| − |
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| − | /* class for a half width column */
| |
| − | .column.half_size {
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| − | width: 50%;
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| − | }
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| − |
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| − | /* styling for images in a half width column*/
| |
| − | .column.half_size img {
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| − | width: 94.5%;
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| − | padding: 10px 15px;
| |
| − | }
| |
| − |
| |
| − | /********************************* SUPPORT CLASSES ********************************/
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| − |
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| − | /* class that clears content below*/
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| − | .igem_2017_content_wrapper .clear {
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| − | clear: both;
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| − | }
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| − |
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| − | /* adds extra spacing when clearing content */
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| − | .igem_2017_content_wrapper .clear.extra_space {
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| − | height: 30px;
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| − | }
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| − |
| |
| − | /* highlight class, makes content slightly smaller */
| |
| − | .igem_2017_content_wrapper .highlight {
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| − | margin: 0px 15px;
| |
| − | padding: 15px 0px;
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| − | }
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| − |
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| − | /* highlight class, adds a gray background */
| |
| − | .igem_2017_content_wrapper .highlight.gray {
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| − | background-color: #190707;
| |
| − | }
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| − |
| |
| − | /* highlight with decoration blue line on top */
| |
| − | .igem_2017_content_wrapper .highlight.blue_top {
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| − | border-top: 4px solid #190707;
| |
| − | }
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| − |
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| − | /* highlight with a full blue border decoration */
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| − | .igem_2017_content_wrapper .highlight.blue_border {
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| − | border: 4px solid #190707;
| |
| − | }
| |
| − |
| |
| − | /* button class */
| |
| − | .igem_2017_content_wrapper .button {
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| − | max-width: 35%;
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| − | margin: 30px auto;
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| − | padding: 12px 10px;
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| − | background-color: #190707;
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| − | text-align: center;
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| − | color: #F8CE63;
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| − | -webkit-transition: all 0.4s ease;
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| − | -moz-transition: all 0.4s ease;
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| − | -ms-transition: all 0.4s ease;
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| − | -o-transition: all 0.4s ease;
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| − | transition: all 0.4s ease;
| |
| − | }
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| − |
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| − | /* styling for button on hover */
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| − | .igem_2017_content_wrapper .button:hover {
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| − | background-color: #190707;
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| − | color: #F8CE63;
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| − | }
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| − |
| |
| − | /***************************************************** RESPONSIVE STYLING ****************************************************/
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| − |
| |
| − | *, *:before, *:after {
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| − | -webkit-box-sizing: border-box;
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| − | -moz-box-sizing: border-box;
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| − | box-sizing: border-box;
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| − | }
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| − |
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| − | body {
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| − | overflow-x: hidden;
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| − | margin-top: 80px;
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| − | letter-spacing: 0.02em;
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| − | }
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| − |
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| − | /* IF THE SCREEN IS LESS THAN 1200PX */
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| − | @media only screen and (max-width: 1024px) {
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| − |
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| − | #content {
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| − | width: 100%;
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| − | }
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| − |
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| − | .igem_2017_content_wrapper {
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| − | width: 100%;
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| − | }
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| − |
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| − | .igem_2017_menu_wrapper {
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| − | width: 100%;
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| − | height: 15%;
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| − | position: relative;
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| − | left: 0;
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| − | }
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| − |
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| − | .highlight {
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| − | padding: 10px 0px;
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| − | }
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| − |
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| − | .igem_2017_menu_wrapper #display_menu_control {
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| − | display: none;
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| − | }
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| − |
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| − | #menu_content {
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| − | display: block;
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| − | }
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| − |
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| − | .menu_button.direct_to_page {
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| − | padding-left: 17px;
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| − | }
| |
| − | }
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| − |
| |
| − | /* IF THE SCREEN IS LESS THAN 800PX */
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| − | @media only screen and (max-width: 800px) {
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| − |
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| − | .igem_2017_content_wrapper {
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| − | width: 100%;
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| − | margin-left: 0;
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| − | }
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| − |
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| − | .column.half_size {
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| − | width: 100%;
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| − | }
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| − |
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| − | .column.full_size img, .column.half_size img {
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| − | width: 100%;
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| − | padding: 10px 0;
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| − | }
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| − |
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| − | .highlight {
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| − | padding: 15px 5px;
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| − | }
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| − |
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| − | .igem_2017_menu_wrapper #display_menu_control {
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| − | display: block;
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| − | }
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| − |
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| − | #menu_content {
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| − | display: none;
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| − | }
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| − |
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| − | .igem_2017_menu_wrapper .menu_button .expand_collapse_icon {
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| − | width: 5%;
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| − | }
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| − |
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| − | .menu_bottom_padding {
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| − | display: none;
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| − | }
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| − |
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| − | .menu_button.direct_to_page {
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| − | padding-left: 36px;
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| − | }
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| − | }
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| − |
| |
| − | /* special class that the system uses to make sure the team wants a page to be evaluated */
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| − | .judges-will-not-evaluate {
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| − | width: 96.6%;
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| − | margin: 5px 15px;
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| − | display: block;
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| − | border: 4px solid #3399ff;
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| − | font-weight: bold;
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| − | }
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| − |
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| − | /* CUSTOM BS NAVBAR STYLES */
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| − | .navbar {
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| − | border: 0;
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| − | }
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| − |
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| − | .navbar-default {
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| − | margin-top: 15px;
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| − | background-color: #333;
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| − | }
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| − |
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| − | .navbar-brand {
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| − | padding: 0;
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| − | padding-left: 25px;
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| − | padding-right: 25px;
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| − | }
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| − |
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| − | .navbar-brand img {
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| − | height: 49px;
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| − | }
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| − |
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| − | .navbar-default .navbar-nav > li > a {
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| − | color: white;
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| − | }
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| − |
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| − | .navbar-default .navbar-nav > li > a:focus,
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| − | .navbar-default .navbar-nav > li > a:hover,
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| − | .navbar-default .navbar-nav > .dropdown > a .caret:hover {
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| − | color: #F8CE63;
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| − | }
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| − |
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| − | .navbar-default .navbar-nav > .open > a,
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| − | .navbar-default .navbar-nav > .open > a:hover,
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| − | .navbar-default .navbar-nav > .open > a:focus {
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| − | color: #F8CE63;
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| − | background-color: #444;
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| − | }
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| − |
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| − | .navbar-default .navbar-nav > .dropdown > a .caret {
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| − | border-top-color: #eee;
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| − | border-bottom-color: #eee;
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| − | }
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| − |
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| − | .dropdown-menu {
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| − | border: 0;
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| − | }
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| − | .dropdown-menu > li > a {
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| − | color: white;
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| − | }
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| − |
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| − | .dropdown-menu > li > a:hover,
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| − | .dropdown-menu > li > a:focus {
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| − | color: #111;
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| − | text-decoration: none;
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| − | background-color: #F8CE63;
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| − | }
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| − |
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| − | .navbar-default .navbar-nav > .dropdown > a:hover .caret,
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| − | .navbar-default .navbar-nav > .dropdown > a:focus .caret {
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| − | border-top-color: white;
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| − | border-bottom-color: white;
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| − | }
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| − |
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| − | @media (max-width: 767px) {
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| − | .navbar-default .navbar-nav .open .dropdown-menu>li>a {
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| − | color: #ddd;
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| − | }
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| − | .navbar-default .navbar-nav .open .dropdown-menu>li>a:focus,
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| − | .navbar-default .navbar-nav .open .dropdown-menu>li>a:hover {
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| − | color: #F8CE63;
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| − | }
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| − | }
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| − |
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| − | .navbar-nav>li>.dropdown-menu {
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| − | background-color: #444;
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| − | }
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| − |
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| − | .section {
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| − | margin: 25px 0;
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| − | }
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| − |
| |
| − | </style>
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| − | <script src="https://code.jquery.com/jquery-1.10.2.min.js"></script>
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| − | <script src="https://netdna.bootstrapcdn.com/bootstrap/3.0.0/js/bootstrap.min.js"></script>
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| − |
| |
| − | <script>
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| − | $(document).ready(function(){
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| − | $("#mw-content-text").removeClass("mw-content-ltr");
| |
| − | });
| |
| − | </script>
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| − |
| |
| − |
| |
| − | <!-- This tells the browser that your page is responsive -->
| |
| − | <meta name="viewport" content="width=device-width, initial-scale=1">
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| − |
| |
| − | </head>
| |
| − | <body>
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| − |
| |
| − | <!--MENU-->
| |
| − | <nav class="navbar navbar-default navbar-fixed-top">
| |
| − | <div class="container-fluid">
| |
| − | <!-- Brand and toggle get grouped for better mobile display -->
| |
| − | <div class="navbar-header">
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| − | <button type="button" class="navbar-toggle collapsed" data-toggle="collapse"
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| − | data-target="#bs-example-navbar-collapse-1" aria-expanded="false">
| |
| − | <span class="sr-only">Toggle navigation</span>
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| − | <span class="icon-bar"></span>
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| − | <span class="icon-bar"></span>
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| − | <span class="icon-bar"></span>
| |
| − | </button>
| |
| − | <a class="navbar-brand" href="https://2017.igem.org/Team:Stuttgart">
| |
| − | <img alt="Team:Stuttgart"
| |
| − | src="https://static.igem.org/mediawiki/2017/1/18/UNISTUTTGARTLOGO.png">
| |
| − | </a>
| |
| − | </div>
| |
| − |
| |
| − | <!-- Collect the nav links, forms, and other content for toggling -->
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| − | <div class="col-xs-12 col-sm- 12 col-md- 12"> | + | <h3>Esterases and Lipases</h3> |
| − | <h3>Esterases and Lipases</h3>
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| + | <div class="col-xs-12 col-sm-2 col-md-2"> |
| − | <p>HIER TEXT REINKOPIEREN</p> | + | <!--<https://static.igem.org/mediawiki/2017/4/45/EsteraseResults.png">--> |
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| + | <img src="https://static.igem.org/mediawiki/2017/7/7f/EsteraseundLipase.png" class="img-responsive"/> |
| − | </div>
| + | </div> |
| − | </div>
| + | <h4>Enzyme activity assay: Esterases</h4> |
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| + | <div class="col-xs-12 col-sm-10 col-md-10"> |
| − | | + | <p> In the following figures enzyme activities of the supernatant of the esterases EstCS2 and LIpB are shown. All results were obtained from biological triplicates. The detailed method for the activity determination is shown here: LINK |
| − | <div class="row section"> | + | At the N-terminal end of the esterase EstCS2 a signal peptide (PelB) is added. Therefore the gene induction leads to enzyme expression and the expression of the signalpeptide and the secretion of the enzyme is enabled. |
| − | <div class="col-xs-12 col-sm- 12 col-md- 12"> | + | </p> |
| − | <h3>Keratinases</h3>
| + | </div> |
| − | <br>
| + | </div> |
| − | <p>HIER TEXT REINKOPIEREN</p>
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| + | <div class="col-xs-7 col-sm-8 col-md-8"> |
| − | </div>
| + | <!--<https://static.igem.org/mediawiki/2017/4/45/EsteraseResults.png">--> |
| − | </div>
| + | <img src="https://static.igem.org/mediawiki/2017/4/45/EsteraseResults.png" class="img-responsive"/> |
| − | | + | <h8>Figure 3: Illustration of the esterase activity [U] of EstCS2 in the supernatant dependend on time and substrate concentration (2.5, 5, 10, 15, 20 mM). The standard abbreviation was calculated from biological triplicates.</h8> |
| − | <div class="row section"> | + | </div> |
| − | <div class="col-xs-12 col-sm- 12 col-md- 12"> | + | <div class="col-xs-5 col-sm-4 col-md-4"> |
| − | <h3>Rose and Limonene Fragrance</h3>
| + | <p>The enzyme activity of EstCS2 raises with the induction level for gene expression and with the substrate concentration. A maximum of 246,5 U is gained with 20 mM p-Nitrophenyl butyrate and 1 mM arabinose. The enzyme activities between the induction levels of 2 mM arabinose and 3 mM arabinose isn’t different. The enzyme activity is highest with an induction level of 1 mM arabinose instead of an induction level of 2 or 3 mM arabinose. This could be explained with less movement flexibility of the esterases if high gene expression rates are induced. We would further recommend to induce the gene expression with less arabinose concentrations and investigate the enzyme activity with 0.1 mM, 0.5 mM and 1 mM arabinose. </p> |
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| + | </div> |
| − | <p> HIER TEXT REINKOPIEREN</p> | + | </div> |
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| − | </div>
| + | <div class="col-xs-12 col-sm- 8 col-md- 8"> |
| − | </div>
| + | <!--<https://static.igem.org/mediawiki/2017/a/a0/EsteraseResults2.png">--> |
| − |
| + | <img src="https://static.igem.org/mediawiki/2017/a/a0/EsteraseResults2.png" class="img-responsive"/> |
| − | | + | <h8>Figure 4: Illustration of the esterase activity [U] of E. coli wilde types in the supernatant dependend on time and substrate concentration (2.5, 5, 10, 15, 20 mM). The standard abbreviation was calculated from biological triplicates. |
| | + | </h8> |
| | + | </div> |
| | + | <div class="col-xs-12 col-sm-4 col-md-4"> |
| | + | <p>In comparison we investigated the enzyme activity of two E. coli wilde type strains (E. coli DH5α and MG1655). We aimed to aquire knowledge about the esterase activity of the wild typs to identify the effect of genetically engineered organisms. The figures show less enzyme activity of the wild types in comparison to the genetically engineered E. coli that contain the plasmid with the esterase genes. This means that the investigated EstCS2 is an appropriate enzyme to degradade fat layers on hair. |
| | + | </p> |
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| | + | <div class="col-xs-12 col-sm-8 col-md-8"> |
| | + | <!--<https://2017.igem.org/File:EsteraseResults3.png">--> |
| | + | <img src="https://static.igem.org/mediawiki/2017/3/3a/EsteraseResults3.png" class="img-responsive"/> |
| | + | <h8>Figure 5:Illustration of the esterase activity [U] of LipB in the supernatant dependend on time and substrate concentration (2.5, 5, 10, 15, 20 mM). The standard abbreviation was calculated from biological triplicates. |
| | + | </h8> |
| | + | </div> |
| | + | <div class="col-xs-12 col-sm-4 col-md-4"> |
| | + | <p> Additionally we investigated the enzyme activity of LipB. To compare the enzyme activities of LipB and EstCS2, we used the same induction levels and substrate concentrations for the assays. The figures show that the enzyme activity of the supernatant isn’t higher than the enzyme activity of the supernatant of the wild type cells. These results can be declared with the absence of a signal peptide at the N-terminal side of the esterase gene. Thus, no enzyme secretion is performed and less enzyme activity can be detected in the supernatant. This leads to the conclusion that a signal peptide has to be added at the N-terminal side of the esterase gene to obtain enzyme secretion and extracellular enzyme activity. |
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