Difference between revisions of "Team:NYMU-Taipei/Parts"

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{{NYMU-Taipei}}
 
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<div class = "igem_2017_content_wrapper">
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<h3></h3>
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<center>
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<img src="https://static.igem.org/mediawiki/2017/4/4d/T--NYMU-Taipei--parts_title.jpeg"
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style="width:45%;border-radius:50%;border:8px solid #412a07;">
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</center>
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<h3></h3>
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<p></p>
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        <div style="min-height:100px;width:800px;background: rgba(100%, 100%, 100%, 0.6);margin:auto;">
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                <table width="700px" border="1" style="background: rgba(100%, 100%, 100%, 0);font-size:20px;">
 +
　              <tr>
 +
              　<td>Part Number </td>
 +
                  <td width="300px"> Description </td>
 +
                  <td > Type </td>
 +
                  <td> Length(bp)</td>
 +
                  <td>  Designer </td>
 +
              　</tr>
 +
　              <tr>
 +
              　<td><a href="http://parts.igem.org/Part:BBa_K2350000"  title="BBa_K2350000">BBa_K2350000</a></td>
 +
                  <td> MelA </td>
 +
                  <td> Coding sequence </td>
 +
                  <td>1833 bp</td>
 +
                  <td>YU-HSIANG LIN</td>
 +
              　</tr>
 +
　              <tr>
 +
              　<td><a href="http://parts.igem.org/Part:BBa_K2350001"  title="BBa_K2350001">BBa_K2350001</a></td>
 +
                  <td>Crt Z (astaxanthin) </td>
 +
                  <td>Coding sequence  </td>
 +
                  <td> 489 bp</td>
 +
                  <td> Priscilla Lee Ann Hweek </td>
 +
              　</tr>
 +
　              <tr>
 +
              　<td><a href="http://parts.igem.org/Part:BBa_K2350002"  title="BBa_K2350002">BBa_K2350002</a></td>
 +
                  <td>Crt W (astaxanthin) </td>
 +
                  <td>Coding sequence  </td>
 +
                  <td> 729 bp</td>
 +
                  <td> Priscilla Lee Ann Hweek </td>
 +
              　</tr>
 +
　              <tr>
 +
              　<td><a href="http://parts.igem.org/Part:BBa_K2350003"  title="BBa_K2350003">BBa_K2350003</a></td>
 +
                  <td>Intrinsic promoter of Rubisco large subunit (PrbcL) </td>
 +
                  <td>Regulatory  </td>
 +
                  <td> 396 bp</td>
 +
                  <td>JO-NING HUNG</td>
 +
              　</tr>
 +
　              <tr>
 +
              　<td><a href="http://parts.igem.org/Part:BBa_K2350004"  title="BBa_K2350004">BBa_K2350004</a></td>
 +
                  <td>A part of nitrate channel protein from Synechocystis sp. PCC 6803 (NrtA) </td>
 +
                  <td>Coding sequence  </td>
 +
                  <td> 1341 bp</td>
 +
                  <td>YEN-CHING TU</td>
 +
              　</tr>
 +
　              <tr>
 +
              　<td><a href="http://parts.igem.org/Part:BBa_K2350005"  title="BBa_K2350005">BBa_K2350005</a></td>
 +
                  <td>3'-ends of the neutral site II (NSII)</td>
 +
                  <td>DNA</td>
 +
                  <td> 1094 bp</td>
 +
                  <td>JO-NING HUNG</td>
 +
              　</tr>
 +
　              <tr>
 +
              　<td><a href="http://parts.igem.org/Part:BBa_K2350006"  title="BBa_K2350006">BBa_K2350006</a></td>
 +
                  <td>5'-ends of the neutral site II (NSII)</td>
 +
                  <td>DNA</td>
 +
                  <td> 1094 bp</td>
 +
                  <td>JO-NING HUNG</td>
 +
              　</tr>
 +
　              <tr>
 +
              　<td><a href="http://parts.igem.org/Part:BBa_K2350007"  title="BBa_K2350007">BBa_K2350007</a></td>
 +
                  <td>Lycopene cyclase upstream in PCC7942</td>
 +
                  <td>Coding sequence</td>
 +
                  <td> 1248 bp</td>
 +
                  <td>CHEN-CHIA JIANG</td>
 +
              　</tr>
 +
　              <tr>
 +
              　<td><a href="http://parts.igem.org/Part:BBa_K2350008"  title="BBa_K2350008">BBa_K2350008</a></td>
 +
                  <td>Lycopene cyclase downstream in PCC7942</td>
 +
                  <td>Coding sequence</td>
 +
                  <td>1214 bp</td>
 +
                  <td>CHEN-CHIA JIANG</td>
 +
              　</tr>
 +
　              <tr>
 +
              　<td><a href="http://parts.igem.org/Part:BBa_K2350009"  title="BBa_K2350009">BBa_K2350009</a></td>
 +
                  <td>Pigment double-crossover homologous gene recombination bacbone (pPIGBACK)</td>
 +
                  <td>Plasmid_Backbone</td>
 +
                  <td>3857 bp</td>
 +
                  <td>JO-NING HUNG</td>
 +
              　</tr>
 +
　              <tr>
 +
              　<td><a href="http://parts.igem.org/Part:BBa_K2350010"  title="BBa_K2350010">BBa_K2350010</a></td>
 +
                  <td>PrbcL-MelA-rrnb</td>
 +
                  <td>Composite</td>
 +
                  <td>2692 bp</td>
 +
                  <td>YU-HSIANG LIN</td>
 +
              　</tr>
 +
　              <tr>
 +
              　<td><a href="http://parts.igem.org/Part:BBa_K2350011"  title="BBa_K2350011">BBa_K2350011</a></td>
 +
                  <td>PrbcL-CrtZ</td>
 +
                  <td>Composite</td>
 +
                  <td>933 bp</td>
 +
                  <td>CHIA-SUI CHIANG</td>
 +
              　</tr>
 +
　              <tr>
 +
              　<td><a href="http://parts.igem.org/Part:BBa_K2350012"  title="BBa_K2350012">BBa_K2350012</a></td>
 +
                  <td>PrbcL-IndC</td>
 +
                  <td>Composite</td>
 +
                  <td>4257 bp</td>
 +
                  <td>JO-NING HUNG</td>
 +
              　</tr>
 +
　              <tr>
 +
              　<td><a href="http://parts.igem.org/Part:BBa_K2350013"  title="BBa_K2350013">BBa_K2350013</a></td>
 +
                  <td>PrbcL-CrtW-CrtZ-rrnB</td>
 +
                  <td>Composite</td>
 +
                  <td>2281 bp</td>
 +
                  <td>Priscilla Lee Ann Hweek</td>
 +
              　</tr>
 +
　              <tr>
 +
              　<td><a href="http://parts.igem.org/Part:BBa_K2350014" title="BBa_K2350014">BBa_K2350014</a></td>
 +
                  <td>Lycopene cyclase upstream + Ampicillin Resistance + Lycopene cyclase downstream</td>
 +
                  <td>Composite</td>
 +
                  <td>3428 bp</td>
 +
                  <td>CHEN-CHIA JIANG</td>
 +
              　</tr>
 +
　              <tr>
 +
              　<td><a href="http://parts.igem.org/Part:BBa_K2350015"  title="BBa_K2350015">BBa_K2350015</a></td>
 +
                  <td>3NSII-ORI-5NSII</td>
 +
                  <td>DNA</td>
 +
                  <td>2762 bp</td>
 +
                  <td>JO-NING HUNG</td>
 +
              　</tr>
 +
　              <tr>
 +
              　<td><a href="http://parts.igem.org/Part:BBa_K2350016"  title="BBa_K2350016">BBa_K2350016</a></td>
 +
                  <td>BBa_B0015 and Ampicillin resistance gene</td>
 +
                  <td>Composite</td>
 +
                  <td>1095 bp</td>
 +
                  <td>JO-NING HUNG</td>
 +
              　</tr>
 +
　              <tr>
 +
              　<td><a href="http://parts.igem.org/Part:BBa_K2350017"  title="BBa_K2350017">BBa_K2350017</a></td>
 +
                  <td>J23118-B0034-NrtA-B0015</td>
 +
                  <td>Composite</td>
 +
                  <td>1539 bp</td>
 +
                  <td>YI-CHIEN LIN</td>
 +
              　</tr>
 +
　              <tr>
 +
              　<td><a href="http://parts.igem.org/Part:BBa_K2350018"  title="BBa_K2350018">BBa_K2350018</a></td>
 +
                  <td> J23106-B0034-Endolysin-B0010-B0012 </td>
 +
                  <td>Composite</td>
 +
                  <td>714 bp</td>
 +
                  <td>PIN-YU CHEN</td>
 +
              　</tr>
 +
　              <tr>
 +
              　<td><a href="http://parts.igem.org/Part:BBa_K2350019"  title="BBa_K2350019">BBa_K2350019</a></td>
 +
                  <td>R0010-B0034-Holin-B0010-B0012</td>
 +
                  <td>Composite</td>
 +
                  <td>1020 bp</td>
 +
                  <td>PIN-YU CHEN</td>
 +
              　</tr>
 +
　              <tr>
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              　<td><a href="http://parts.igem.org/Part:BBa_K2350020"  title="BBa_K2350020">BBa_K2350020</a></td>
 +
                  <td>J23106-B0034-Endolysin-B0010-B0012-R0010-B0034-Holin-B0010-B0012</td>
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                  <td>Composite</td>
 +
                  <td>1742 bp</td>
 +
                  <td>YA-XUAN YANG</td>
 +
              　</tr>
 +
　              <tr>
 +
              　<td><a href="http://parts.igem.org/Part:BBa_K2350021"  title="BBa_K2350021">BBa_K2350021</a></td>
 +
                  <td>J23106-B0034-Endolysin-B0010-B0012-R0010-B0034-Holin-B0010-B0012-J23118-B0034-NrtA-B0015</td>
 +
                  <td>Composite</td>
 +
                  <td>1970 bp</td>
 +
                  <td>YA-XUAN YANG</td>
 +
              　</tr>
 +
　              <tr>
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              　<td><a href="http://parts.igem.org/Part:BBa_K2350022"  title="BBa_K2350022">BBa_K2350022</a></td>
 +
                  <td>pPIGBACK-PrbcL-CrtZ</td>
 +
                  <td>Composite</td>
 +
                  <td>933 bp</td>
 +
                  <td> CHIA-SUI CHIANG</td>
 +
              　</tr>
 +
　              <tr>
 +
              　<td><a href="http://parts.igem.org/Part:BBa_K2350023"  title="BBa_K2350023">BBa_K2350023</a></td>
 +
                  <td>pPIGBACK-PrbcL-CrtW-CrtZ-rrnB</td>
 +
                  <td>Composite</td>
 +
                  <td>6164 bp</td>
 +
                  <td>Priscilla Lee Ann Hweek</td>
 +
              　</tr>
 +
　              <tr>
 +
              　<td><a href="http://parts.igem.org/Part:BBa_K2350024"  title="BBa_K2350024">BBa_K2350024</a></td>
 +
                  <td>pPIGBACK-PrbcL-MelA-rrnb</td>
 +
                  <td>Composite</td>
 +
                  <td>6557 bp</td>
 +
                  <td>YU-HSIANG LIN</td>
 +
              　</tr>
 +
　              <tr>
 +
              　<td><a href="http://parts.igem.org/Part:BBa_K2350025"  title="BBa_K2350025">BBa_K2350025</a></td>
 +
                  <td>pPIGBACK-PrbcL-IndC</td>
 +
                  <td>Composite</td>
 +
                  <td>8122 bp</td>
 +
                  <td>JO-NING HUNG</td>
 +
              　</tr>
 +
                </table>
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<p></p>
  
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</div>
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<div style="width:800px;margin:auto">
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<p>　　Our part collection included basic parts and composite parts. We focused on two aspects, one, nitrogen affinity protein for nitrogen starvation and two, pigment production in cyanobacteria. These two approaches were aimed to produce more energy from microalgae by <font class="mark_backbone">nitrogen starvation</font> and <font class="mark_backbone">artificial photosynthesis</font>. We also implemented <font class="mark_backbone">suicide mechanism</font> by combining holin and endolysin with nitrogen affinity protein in one construct for issues regarding biosafety. We successfully proved the function of nitrogen affinity protein, suicide mechanism and yellow pigment production in E. coli and cyanobacteria. We also set up a <font class="mark_backbone">transformation platform</font> for cyanobacteria with these new transformation parts and protocols. In conclusion, we proved our concept by this part collection from BBa_K2350000 to BBa_K2350025.
 +
</p>
 +
<h6>Favorate Basic Parts :  <a href="https://2017.igem.org/Team:NYMU-Taipei/Basic_Part">BBa_K2350004</a></h6>
 +
<h6>Favorate Composite Parts :  <a href="https://2017.igem.org/Team:NYMU-Taipei/Composite_Part">BBa_K2350022</a></h6>
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<h1>Parts</h1>
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<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
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<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
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<h5>Note</h5>
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<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
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<h5>Adding parts to the registry</h5>
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<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
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<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
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<h5>What information do I need to start putting my parts on the Registry?</h5>
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<p>The information needed to initially create a part on the Registry is:</p>
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<ul>
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<li>Part Name</li>
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<li>Part type</li>
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<li>Creator</li>
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<li>Sequence</li>
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<li>Short Description (60 characters on what the DNA does)</li>
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<li>Long Description (Longer description of what the DNA does)</li>
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<li>Design considerations</li>
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<p>
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We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
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<h5>Inspiration</h5>
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<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
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<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
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<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
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<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
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<h5>Part Table </h5>
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<p>Please include a table of all the parts your team has made during your project on this page. Remember part characterization and measurement data must go on your team part pages on the Registry. </p>
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<groupparts>iGEM17 NYMU-Taipei</groupparts>
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Latest revision as of 11:07, 30 October 2017

HOME
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Email us: 2017igem.nymutaipei@gmail.com
Call us: 886-2-28267316
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AFFILIATIONS & ACKNOWLEDGMENT

IGEM 2017
National Yang-Ming University
Special Thanks

　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　
Part Number Description Type Length(bp) Designer
BBa_K2350000 MelA Coding sequence 1833 bp YU-HSIANG LIN
BBa_K2350001 Crt Z (astaxanthin) Coding sequence 489 bp Priscilla Lee Ann Hweek
BBa_K2350002 Crt W (astaxanthin) Coding sequence 729 bp Priscilla Lee Ann Hweek
BBa_K2350003 Intrinsic promoter of Rubisco large subunit (PrbcL) Regulatory 396 bp JO-NING HUNG
BBa_K2350004 A part of nitrate channel protein from Synechocystis sp. PCC 6803 (NrtA) Coding sequence 1341 bp YEN-CHING TU
BBa_K2350005 3'-ends of the neutral site II (NSII) DNA 1094 bp JO-NING HUNG
BBa_K2350006 5'-ends of the neutral site II (NSII) DNA 1094 bp JO-NING HUNG
BBa_K2350007 Lycopene cyclase upstream in PCC7942 Coding sequence 1248 bp CHEN-CHIA JIANG
BBa_K2350008 Lycopene cyclase downstream in PCC7942 Coding sequence 1214 bp CHEN-CHIA JIANG
BBa_K2350009 Pigment double-crossover homologous gene recombination bacbone (pPIGBACK) Plasmid_Backbone 3857 bp JO-NING HUNG
BBa_K2350010 PrbcL-MelA-rrnb Composite 2692 bp YU-HSIANG LIN
BBa_K2350011 PrbcL-CrtZ Composite 933 bp CHIA-SUI CHIANG
BBa_K2350012 PrbcL-IndC Composite 4257 bp JO-NING HUNG
BBa_K2350013 PrbcL-CrtW-CrtZ-rrnB Composite 2281 bp Priscilla Lee Ann Hweek
BBa_K2350014 Lycopene cyclase upstream + Ampicillin Resistance + Lycopene cyclase downstream Composite 3428 bp CHEN-CHIA JIANG
BBa_K2350015 3NSII-ORI-5NSII DNA 2762 bp JO-NING HUNG
BBa_K2350016 BBa_B0015 and Ampicillin resistance gene Composite 1095 bp JO-NING HUNG
BBa_K2350017 J23118-B0034-NrtA-B0015 Composite 1539 bp YI-CHIEN LIN
BBa_K2350018 J23106-B0034-Endolysin-B0010-B0012 Composite 714 bp PIN-YU CHEN
BBa_K2350019 R0010-B0034-Holin-B0010-B0012 Composite 1020 bp PIN-YU CHEN
BBa_K2350020 J23106-B0034-Endolysin-B0010-B0012-R0010-B0034-Holin-B0010-B0012 Composite 1742 bp YA-XUAN YANG
BBa_K2350021 J23106-B0034-Endolysin-B0010-B0012-R0010-B0034-Holin-B0010-B0012-J23118-B0034-NrtA-B0015 Composite 1970 bp YA-XUAN YANG
BBa_K2350022 pPIGBACK-PrbcL-CrtZ Composite 933 bp CHIA-SUI CHIANG
BBa_K2350023 pPIGBACK-PrbcL-CrtW-CrtZ-rrnB Composite 6164 bp Priscilla Lee Ann Hweek
BBa_K2350024 pPIGBACK-PrbcL-MelA-rrnb Composite 6557 bp YU-HSIANG LIN
BBa_K2350025 pPIGBACK-PrbcL-IndC Composite 8122 bp JO-NING HUNG

　　Our part collection included basic parts and composite parts. We focused on two aspects, one, nitrogen affinity protein for nitrogen starvation and two, pigment production in cyanobacteria. These two approaches were aimed to produce more energy from microalgae by nitrogen starvation and artificial photosynthesis. We also implemented suicide mechanism by combining holin and endolysin with nitrogen affinity protein in one construct for issues regarding biosafety. We successfully proved the function of nitrogen affinity protein, suicide mechanism and yellow pigment production in E. coli and cyanobacteria. We also set up a transformation platform for cyanobacteria with these new transformation parts and protocols. In conclusion, we proved our concept by this part collection from BBa_K2350000 to BBa_K2350025.

Favorate Basic Parts : BBa_K2350004
Favorate Composite Parts : BBa_K2350022