Difference between revisions of "Team:NYMU-Taipei/Parts"

 
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  <h5>Part Table </h5>
 
  
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  <div class="highlight">
 
  
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         <div id='groupparts' style='min-height:100px;width:700px;'
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         <div style="min-height:100px;width:800px;background: rgba(100%, 100%, 100%, 0.6);margin:auto;">
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                <table width="800px" border="1">
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                <table width="700px" border="1" style="background: rgba(100%, 100%, 100%, 0);font-size:20px;">
 
　              <tr>
 
　              <tr>
 
               　<td>Part Number </td>
 
               　<td>Part Number </td>
−
                  <td>Type </td>
+
                  <td width="300px"> Description </td>
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                   <td> Description </td>
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                   <td > Type </td>
 
                   <td> Length(bp)</td>
 
                   <td> Length(bp)</td>
 
                   <td>  Designer </td>
 
                   <td>  Designer </td>
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　              <tr>
 
　              <tr>
 
               　<td><a href="http://parts.igem.org/Part:BBa_K2350004"  title="BBa_K2350004">BBa_K2350004</a></td>
 
               　<td><a href="http://parts.igem.org/Part:BBa_K2350004"  title="BBa_K2350004">BBa_K2350004</a></td>
−
                   <td>a part of nitrate channel protein from Synechocystis sp. PCC 6803 </td>
+
                   <td>A part of nitrate channel protein from Synechocystis sp. PCC 6803 (NrtA) </td>
 
                   <td>Coding sequence  </td>
 
                   <td>Coding sequence  </td>
 
                   <td> 1341 bp</td>
 
                   <td> 1341 bp</td>
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　              <tr>
 
　              <tr>
 
               　<td><a href="http://parts.igem.org/Part:BBa_K2350005"  title="BBa_K2350005">BBa_K2350005</a></td>
 
               　<td><a href="http://parts.igem.org/Part:BBa_K2350005"  title="BBa_K2350005">BBa_K2350005</a></td>
−
                   <td>3’-ends of the neutral site II (NSII)</td>
+
                   <td>3'-ends of the neutral site II (NSII)</td>
 
                   <td>DNA</td>
 
                   <td>DNA</td>
 
                   <td> 1094 bp</td>
 
                   <td> 1094 bp</td>
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　              <tr>
 
　              <tr>
 
               　<td><a href="http://parts.igem.org/Part:BBa_K2350006"  title="BBa_K2350006">BBa_K2350006</a></td>
 
               　<td><a href="http://parts.igem.org/Part:BBa_K2350006"  title="BBa_K2350006">BBa_K2350006</a></td>
−
                   <td>5’-ends of the neutral site II (NSII)</td>
+
                   <td>5'-ends of the neutral site II (NSII)</td>
 
                   <td>DNA</td>
 
                   <td>DNA</td>
 
                   <td> 1094 bp</td>
 
                   <td> 1094 bp</td>
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　              <tr>
 
　              <tr>
 
               　<td><a href="http://parts.igem.org/Part:BBa_K2350008"  title="BBa_K2350008">BBa_K2350008</a></td>
 
               　<td><a href="http://parts.igem.org/Part:BBa_K2350008"  title="BBa_K2350008">BBa_K2350008</a></td>
−
                   <td>NONE</td>
+
                   <td>Lycopene cyclase downstream in PCC7942</td>
−
                   <td>NONE</td>
+
                   <td>Coding sequence</td>
−
                   <td>NOENE</td>
+
                   <td>1214 bp</td>
−
                   <td>NONE</td>
+
                   <td>CHEN-CHIA JIANG</td>
 
               　</tr>
 
               　</tr>
 
　              <tr>
 
　              <tr>
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　              <tr>
 
　              <tr>
 
               　<td><a href="http://parts.igem.org/Part:BBa_K2350010"  title="BBa_K2350010">BBa_K2350010</a></td>
 
               　<td><a href="http://parts.igem.org/Part:BBa_K2350010"  title="BBa_K2350010">BBa_K2350010</a></td>
−
                   <td>PrbcL+MelA+rrnb</td>
+
                   <td>PrbcL-MelA-rrnb</td>
 
                   <td>Composite</td>
 
                   <td>Composite</td>
 
                   <td>2692 bp</td>
 
                   <td>2692 bp</td>
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　              <tr>
 
　              <tr>
 
               　<td><a href="http://parts.igem.org/Part:BBa_K2350011"  title="BBa_K2350011">BBa_K2350011</a></td>
 
               　<td><a href="http://parts.igem.org/Part:BBa_K2350011"  title="BBa_K2350011">BBa_K2350011</a></td>
−
                   <td>NONE</td>
+
                   <td>PrbcL-CrtZ</td>
−
                   <td>NONE</td>
+
                   <td>Composite</td>
−
                   <td>NOENE</td>
+
                   <td>933 bp</td>
−
                   <td>NONE</td>
+
                   <td>CHIA-SUI CHIANG</td>
 
               　</tr>
 
               　</tr>
 
　              <tr>
 
　              <tr>
 
               　<td><a href="http://parts.igem.org/Part:BBa_K2350012"  title="BBa_K2350012">BBa_K2350012</a></td>
 
               　<td><a href="http://parts.igem.org/Part:BBa_K2350012"  title="BBa_K2350012">BBa_K2350012</a></td>
−
                   <td>PrbcL+MelA+rrnb</td>
+
                   <td>PrbcL-IndC</td>
 
                   <td>Composite</td>
 
                   <td>Composite</td>
 
                   <td>4257 bp</td>
 
                   <td>4257 bp</td>
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               　</tr>
 
               　</tr>
 
　              <tr>
 
　              <tr>
−
               　<td><a href="http://parts.igem.org/Part:BBa_K2350014 title="BBa_K2350014">BBa_K2350014</a></td>
+
               　<td><a href="http://parts.igem.org/Part:BBa_K2350014" title="BBa_K2350014">BBa_K2350014</a></td>
−
                   <td>pPIGBACK-PrbcL-CrtW-CrtZ-rrnB</td>
+
                   <td>Lycopene cyclase upstream + Ampicillin Resistance + Lycopene cyclase downstream</td>
 
                   <td>Composite</td>
 
                   <td>Composite</td>
−
                   <td>5636 bp</td>
+
                   <td>3428 bp</td>
−
                   <td>Priscilla Lee Ann Hweek</td>
+
                   <td>CHEN-CHIA JIANG</td>
 
               　</tr>
 
               　</tr>
 
　              <tr>
 
　              <tr>
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               　<td><a href="http://parts.igem.org/Part:BBa_K2350021"  title="BBa_K2350021">BBa_K2350021</a></td>
 
               　<td><a href="http://parts.igem.org/Part:BBa_K2350021"  title="BBa_K2350021">BBa_K2350021</a></td>
 
                   <td>J23106-B0034-Endolysin-B0010-B0012-R0010-B0034-Holin-B0010-B0012-J23118-B0034-NrtA-B0015</td>
 
                   <td>J23106-B0034-Endolysin-B0010-B0012-R0010-B0034-Holin-B0010-B0012-J23118-B0034-NrtA-B0015</td>
−
                   <td>Coding sequence</td>
+
                   <td>Composite</td>
 
                   <td>1970 bp</td>
 
                   <td>1970 bp</td>
 
                   <td>YA-XUAN YANG</td>
 
                   <td>YA-XUAN YANG</td>
 +
              　</tr>
 +
　              <tr>
 +
              　<td><a href="http://parts.igem.org/Part:BBa_K2350022"  title="BBa_K2350022">BBa_K2350022</a></td>
 +
                  <td>pPIGBACK-PrbcL-CrtZ</td>
 +
                  <td>Composite</td>
 +
                  <td>933 bp</td>
 +
                  <td> CHIA-SUI CHIANG</td>
 +
              　</tr>
 +
　              <tr>
 +
              　<td><a href="http://parts.igem.org/Part:BBa_K2350023"  title="BBa_K2350023">BBa_K2350023</a></td>
 +
                  <td>pPIGBACK-PrbcL-CrtW-CrtZ-rrnB</td>
 +
                  <td>Composite</td>
 +
                  <td>6164 bp</td>
 +
                  <td>Priscilla Lee Ann Hweek</td>
 +
              　</tr>
 +
　              <tr>
 +
              　<td><a href="http://parts.igem.org/Part:BBa_K2350024"  title="BBa_K2350024">BBa_K2350024</a></td>
 +
                  <td>pPIGBACK-PrbcL-MelA-rrnb</td>
 +
                  <td>Composite</td>
 +
                  <td>6557 bp</td>
 +
                  <td>YU-HSIANG LIN</td>
 +
              　</tr>
 +
　              <tr>
 +
              　<td><a href="http://parts.igem.org/Part:BBa_K2350025"  title="BBa_K2350025">BBa_K2350025</a></td>
 +
                  <td>pPIGBACK-PrbcL-IndC</td>
 +
                  <td>Composite</td>
 +
                  <td>8122 bp</td>
 +
                  <td>JO-NING HUNG</td>
 
               　</tr>
 
               　</tr>
 
                 </table>
 
                 </table>
 +
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<div style="width:800px;margin:auto">
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<p>　　Our part collection included basic parts and composite parts. We focused on two aspects, one, nitrogen affinity protein for nitrogen starvation and two, pigment production in cyanobacteria. These two approaches were aimed to produce more energy from microalgae by <font class="mark_backbone">nitrogen starvation</font> and <font class="mark_backbone">artificial photosynthesis</font>. We also implemented <font class="mark_backbone">suicide mechanism</font> by combining holin and endolysin with nitrogen affinity protein in one construct for issues regarding biosafety. We successfully proved the function of nitrogen affinity protein, suicide mechanism and yellow pigment production in E. coli and cyanobacteria. We also set up a <font class="mark_backbone">transformation platform</font> for cyanobacteria with these new transformation parts and protocols. In conclusion, we proved our concept by this part collection from BBa_K2350000 to BBa_K2350025.
 +
</p>
 +
<h6>Favorate Basic Parts :  <a href="https://2017.igem.org/Team:NYMU-Taipei/Basic_Part">BBa_K2350004</a></h6>
 +
<h6>Favorate Composite Parts :  <a href="https://2017.igem.org/Team:NYMU-Taipei/Composite_Part">BBa_K2350022</a></h6>
 +
</div>
  
 
</div>
 
</div>

Latest revision as of 11:07, 30 October 2017

HOME
PROJECT
Overview
Pigments
Nitrogen starvation
Modeling
Hardware
Contribution
Improvement
Demonstration
LABWORK
Parts
Safety
Notebook
HUMAN PRACTICES
Silver HP
Gold & Integrated HP
Collaborations
ABOUT US
AWARDS
Applied Design
Modeling
Measurement
Plant
Basic Parts
Composite Parts
Part Collection
Public Engagement
JUDGING FORM

CONTACT US

Email us: 2017igem.nymutaipei@gmail.com
Call us: 886-2-28267316
Facebook: NYMU iGEM Team

AFFILIATIONS & ACKNOWLEDGMENT

IGEM 2017
National Yang-Ming University
Special Thanks

　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　
Part Number Description Type Length(bp) Designer
BBa_K2350000 MelA Coding sequence 1833 bp YU-HSIANG LIN
BBa_K2350001 Crt Z (astaxanthin) Coding sequence 489 bp Priscilla Lee Ann Hweek
BBa_K2350002 Crt W (astaxanthin) Coding sequence 729 bp Priscilla Lee Ann Hweek
BBa_K2350003 Intrinsic promoter of Rubisco large subunit (PrbcL) Regulatory 396 bp JO-NING HUNG
BBa_K2350004 A part of nitrate channel protein from Synechocystis sp. PCC 6803 (NrtA) Coding sequence 1341 bp YEN-CHING TU
BBa_K2350005 3'-ends of the neutral site II (NSII) DNA 1094 bp JO-NING HUNG
BBa_K2350006 5'-ends of the neutral site II (NSII) DNA 1094 bp JO-NING HUNG
BBa_K2350007 Lycopene cyclase upstream in PCC7942 Coding sequence 1248 bp CHEN-CHIA JIANG
BBa_K2350008 Lycopene cyclase downstream in PCC7942 Coding sequence 1214 bp CHEN-CHIA JIANG
BBa_K2350009 Pigment double-crossover homologous gene recombination bacbone (pPIGBACK) Plasmid_Backbone 3857 bp JO-NING HUNG
BBa_K2350010 PrbcL-MelA-rrnb Composite 2692 bp YU-HSIANG LIN
BBa_K2350011 PrbcL-CrtZ Composite 933 bp CHIA-SUI CHIANG
BBa_K2350012 PrbcL-IndC Composite 4257 bp JO-NING HUNG
BBa_K2350013 PrbcL-CrtW-CrtZ-rrnB Composite 2281 bp Priscilla Lee Ann Hweek
BBa_K2350014 Lycopene cyclase upstream + Ampicillin Resistance + Lycopene cyclase downstream Composite 3428 bp CHEN-CHIA JIANG
BBa_K2350015 3NSII-ORI-5NSII DNA 2762 bp JO-NING HUNG
BBa_K2350016 BBa_B0015 and Ampicillin resistance gene Composite 1095 bp JO-NING HUNG
BBa_K2350017 J23118-B0034-NrtA-B0015 Composite 1539 bp YI-CHIEN LIN
BBa_K2350018 J23106-B0034-Endolysin-B0010-B0012 Composite 714 bp PIN-YU CHEN
BBa_K2350019 R0010-B0034-Holin-B0010-B0012 Composite 1020 bp PIN-YU CHEN
BBa_K2350020 J23106-B0034-Endolysin-B0010-B0012-R0010-B0034-Holin-B0010-B0012 Composite 1742 bp YA-XUAN YANG
BBa_K2350021 J23106-B0034-Endolysin-B0010-B0012-R0010-B0034-Holin-B0010-B0012-J23118-B0034-NrtA-B0015 Composite 1970 bp YA-XUAN YANG
BBa_K2350022 pPIGBACK-PrbcL-CrtZ Composite 933 bp CHIA-SUI CHIANG
BBa_K2350023 pPIGBACK-PrbcL-CrtW-CrtZ-rrnB Composite 6164 bp Priscilla Lee Ann Hweek
BBa_K2350024 pPIGBACK-PrbcL-MelA-rrnb Composite 6557 bp YU-HSIANG LIN
BBa_K2350025 pPIGBACK-PrbcL-IndC Composite 8122 bp JO-NING HUNG

　　Our part collection included basic parts and composite parts. We focused on two aspects, one, nitrogen affinity protein for nitrogen starvation and two, pigment production in cyanobacteria. These two approaches were aimed to produce more energy from microalgae by nitrogen starvation and artificial photosynthesis. We also implemented suicide mechanism by combining holin and endolysin with nitrogen affinity protein in one construct for issues regarding biosafety. We successfully proved the function of nitrogen affinity protein, suicide mechanism and yellow pigment production in E. coli and cyanobacteria. We also set up a transformation platform for cyanobacteria with these new transformation parts and protocols. In conclusion, we proved our concept by this part collection from BBa_K2350000 to BBa_K2350025.

Favorate Basic Parts : BBa_K2350004
Favorate Composite Parts : BBa_K2350022