Difference between revisions of "Team:USTC/Composite Part"

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{{USTC}}
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<h3>★  ALERT! </h3>
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font-size: 13px!important;
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<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
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font-weight: bold;
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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<div class="column full_size">
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<h1>Composite Parts</h1>
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<p>
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A composite part is a functional unit of DNA consisting of two or more basic parts assembled together. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_I13507">BBa_I13507</a> is an example of a composite part, consisting of an RBS, a protein coding region for a red fluorescent protein, and a terminator.
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</script>
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</p>
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<p>New composite BioBrick devices can be made by combining existing BioBrick Parts (like Inverters, Amplifiers, Smell Generators, Protein Balloon Generators, Senders, Receivers, Actuators, and so on).</p>
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</head>
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<body>
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<header>
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<nav class="top-nav">
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<div class="container">
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<div class="nav-wrapper"><a class="page-title">Composite Parts</a>
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</div>
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</div>
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</nav>
  
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<br>
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<div class="container">
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<h3>Best Composite Part Special Prize</h3>
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<a href="#" data-activates="nav-mobile" class="button-collapse top-nav full hide-on-large-only ">
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<img src="https://static.igem.org/mediawiki/2017/a/a2/Ustc_2017_menu_32px.png">
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</a>
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</div>
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<ul id="nav-mobile" class="side-nav fixed">
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<li class="logo" style="border-bottom: 2px solid #ee6e73">
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<div class="logo-container" >
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<img src="https://static.igem.org/mediawiki/2017/thumb/8/89/Ustc_2017team_logo.png/900px-Ustc_2017team_logo.png" width="100%" height="100%">
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</div>
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</li>
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<li class="bold1 lavender_choosed">
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<a href="https://2017.igem.org/Team:USTC" class="waves-effect waves-teal">Home</a>
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</li>
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<p>New BioBrick devices can be made by combining existing BioBrick Parts. For example, Inverters, Amplifiers, Smell Generators, Protein Balloon Generators, Senders, Receivers, Actuators, and so on. To be eligible for this award, this part must adhere to <a href="http://parts.igem.org/DNA_Submission">Registry sample submission guidelines</a> and have been sent to the Registry of Standard Biological Parts. If you have a part you wish to nominate your team for this <a href="https://2017.igem.org/Judging/Awards">special prize</a>, make sure you add your part number to your <a href="https://2017.igem.org/Judging/Judging_Form">judging form</a> and delete the box at the top of this page.
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<li class="no-padding">
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          <ul class="collapsible collapsible-accordion">
  
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<br><br>
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            <li class="bold Aliceblue_choosed"><a class="collapsible-header  waves-effect waves-teal">Project</a>
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<b>Please note:</b> Judges will only look at the first part number you list, so please only enter ONE (1) part number in the judging form for this prize. </p>
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              <div class="collapsible-body">
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<br>
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                <ul>
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<div class="highlight">
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                  <li class="bg_all"><a href="https://2017.igem.org/Team:USTC/Description">Description</a></li>
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<h4>Note</h4>
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                  <li class="bg_all"><a href="https://2017.igem.org/Team:USTC/Design">Design</a></li>
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<p>This page should list all the composite parts your team has made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page. Remember judges will only look at the first part in the list for the Best Composite Part award, so put your best part first!</p>
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                  <li class="bg_all"><a href="https://2017.igem.org/Team:USTC/Demonstrate">Results</a></li>
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                  <li><a href="https://2017.igem.org/Team:USTC/Demonstrate/1">&nbsp;&nbsp;>Conduction</a></li>  
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                  <li><a href="https://2017.igem.org/Team:USTC/Demonstrate/2">&nbsp;&nbsp;>Photocatalyst</a></li>
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                  <li><a href="https://2017.igem.org/Team:USTC/Demonstrate/3">&nbsp;&nbsp;>Harvest</a></li>
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                </ul>
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              </div>
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            </li>
  
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</div>
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            <li class="bold1 azure_choosed">
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<a href="https://2017.igem.org/Team:USTC/Safety" class="waves-effect waves-teal">Safety</a>
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</li>
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<li class="bold honeydew_choosed"><a class="collapsible-header  waves-effect waves-teal">Model</a>
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              <div class="collapsible-body">
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                <ul>
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                  <li class="bg_all"><a href="https://2017.igem.org/Team:USTC/Model">Overview</a></li>
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                  <li class="bg_all"><a href="https://2017.igem.org/Team:USTC/Model/2">DLA Crystal</a></li>
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                  <li id="static_word" class="bg_all"><a>Electron transfer</a></li>
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                  <li><a href="https://2017.igem.org/Team:USTC/Model/4">&nbsp;&nbsp;>Semi-conductor</a></li>
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                  <li><a href="https://2017.igem.org/Team:USTC/Model/3">&nbsp;&nbsp;>Markov</a></li>
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                  <li><a href="https://2017.igem.org/Team:USTC/Model/1">&nbsp;&nbsp;>MeCiM</a></li>
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                  <li class="bg_all"><a href="https://2017.igem.org/Team:USTC/Model/5">UPEP</a></li>
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                </ul>
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              </div>
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            </li>
  
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</div>
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            <li class="bold honeydew_choosed"><a class="collapsible-header  waves-effect waves-teal">Parts</a>
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              <div class="collapsible-body">
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                <ul>
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                  <li><a href="https://2017.igem.org/Team:USTC/Parts">Parts</a></li>
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                  <li><a href="https://2017.igem.org/Team:USTC/Basic_Part">Basic Parts</a></li>
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                  <li><a href="https://2017.igem.org/Team:USTC/Composite_Part">Composite Parts</a></li>
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                  <li><a href="https://2017.igem.org/Team:USTC/Contribution">Contributions</a></li>
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                  <li><a href="https://2017.igem.org/Team:USTC/Improve">Improve</a></li>
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                </ul>
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              </div>
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            </li>
  
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            <li class="bold ivory_choosed"><a class="collapsible-header waves-effect waves-teal">Notebook</a>
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              <div class="collapsible-body">
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                <ul>
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                  <li><a href="https://2017.igem.org/Team:USTC/Notebook">Experiment Log</a></li>
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                  <li><a href="https://2017.igem.org/Team:USTC/Experiments">Experiments</a></li>
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                </ul>
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              </div>
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            </li>
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            <li class="bold lemonchiffon_choosed"><a class="collapsible-header  waves-effect waves-teal">Human Practice</a>
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              <div class="collapsible-body">
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                <ul>
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                  <li><a href="https://2017.igem.org/Team:USTC/HP/Silver">Silver HP</a></li>
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                  <li style="font-size: 16px"><a href="https://2017.igem.org/Team:USTC/HP/Gold_Integrated">Integrated and Gold</a></li>
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                  <li><a href="https://2017.igem.org/Team:USTC/Engagement">Public Engagement</a></li>
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                </ul>
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              </div>
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            </li>
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            <li class="bold1 cornsilk_choosed">
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<a href="https://2017.igem.org/Team:USTC/Collaborations" class="waves-effect waves-teal">Collaborations</a>
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</li>
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<li class="bold1 seashell_choosed">
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<a href="https://2017.igem.org/Team:USTC/Achievements" class="waves-effect waves-teal">Achievements</a>
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</li>
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<li class="bold1 lavenderblush_choosed">
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<a href="https://2017.igem.org/Team:USTC/Team" class="waves-effect waves-teal">Team</a>
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</li>
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<li class="bold1 mistyrose_choosed">
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<a href="https://2017.igem.org/Team:USTC/Attributions" class="waves-effect waves-teal">Attributions</a>
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</li>
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          </ul>
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        </li>
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</ul>
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</header>
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<main>
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<div class="container">
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<div class="row">
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<div class="col s12 m11 110">
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<div >
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<p><br>This year we, team USTC, submitted 7 basic parts. All of them are coding part as we did not use any special promoters or RBS in our project. These parts are different proteins that we have used in our project under control of different promoter or operator.</p>
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</div>
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<div >
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                                        <table>
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                                                  <tr>
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                                                      <th>Part’s Number</th>
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                                                      <th>Part’s Name</th>
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                                                      <th>Type</th>
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                                                      <th>Length</th>
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                                                      <th>Function</th>
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                                                      <th>Source</th>
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                                                      <th>Safety Issues</th>
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                                                  </tr>
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                                                  <tr>
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                                                    <td><a href="http://parts.igem.org/Part:BBa_K2242920">BBa_K2242920</a></td>
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                                                    <td>araC+pBAD+CmCR</td>
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                                                    <td>Coding</td>
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                                                    <td>2089</td>
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                                                    <td>To induce the gene expression, we use a promotor called pBAD, which is induced by arabinose.</td>
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                                                    <td>The plasmid is bought from a bio-company. The reductase CmCR’s gene is kindly provided by Prof.HongJiong. The recombinant plasmid is constructed by ourselves.</td>
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                                                    <td>The substrate of this reductase is COBE, which is an organic compound with a pungent smell. More importantly, it’s harmful to our skin and respiratory tract. So during the experiment process, we need to take up certain measures to protect ourselves.</td>
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                                                  </tr>
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                                                  <tr>
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                                                    <td><a href="http://parts.igem.org/Part:BBa_K2242521">BBa_K2242521</a></td>
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                                                    <td>placI+lacI+pTAC+CmCR</td>
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                                                    <td>Coding</td>
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                                                    <td>2326</td>
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                                                    <td>We use a promoter pTAC to control the gene CmCR’s expression, which is induced by IPTG.</td>
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                                                    <td>The plasmid backbone pYYDT is kindly provided by Prof. Yu Hanqing. The gene CmCR is kindly provided by Prof. Hong Jiong. The recombinant plasmid is constructed by ourselves.</td>
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                                                    <td>The substrate of this reductase is COBE, which is an organic compound with a pungent smell. More importantly, it’s harmful to our skin and respiratory tract. So during the experiment process, we need to take up certain measures to protect ourselves.</td>
 +
                                                  </tr>
 +
                                                  <tr>
 +
                                                    <td><a href="http://parts.igem.org/Part:BBa_K2242018">BBa_K2242018</a></td>
 +
                                                    <td>T7+LacO+Mtr</td>
 +
                                                    <td>Coding</td>
 +
                                                    <td>5272</td>
 +
                                                    <td>We use T7 promoter and Lac operator to control Mtr CAB’s expression. Both of this two units are induced by IPTG. With this dual switch, we can reduce the leak of the gene expression as much as possible.</td>
 +
                                                    <td>The plasmid is bought from a bio-company. The Mtr gene is obtained from the kit plate. The recombinant plasmid is constructed by ourselves.</td>
 +
                                                    <td></td>
 +
                                                  </tr>
 +
                                                  <tr>
 +
                                                    <td><a href="http://parts.igem.org/Part:BBa_K2242419">BBa_K2242419</td>
 +
                                                    <td>pTET+ccm</td>
 +
                                                    <td>Coding</td>
 +
                                                    <td>6372</td>
 +
                                                    <td>We use a constitutively on promoter pTET to control gene ccmA-H’s expression.</td>
 +
                                                    <td>The plasmid backbone and the promoter pTET is from the kit plate. The ccm A-H gene is amplified from the genome of an E.coli strain BL21(DE3). The recombinant plasmid is constructed by ourselves.</td>
 +
                                                    <td></td>
 +
                                                  </tr>
 +
                                                  <tr>
 +
                                                    <td><a href="http://parts.igem.org/Part:BBa_K2242633">BBa_K2242633</a></td>
 +
                                                    <td>pLuxR+CysDes</td>
 +
                                                    <td>Coding</td>
 +
                                                    <td>2367</td>
 +
                                                    <td>We use the pLuxR promoter from the quorum sensing system, which can be induced by AHL.</td>
 +
                                                    <td>The plasmid backbone and the promoter pLuxR is from the kit plate. The CysDes gene is synthesized by IDT. The recombinant plasmid is constructed by ourselves.</td>
 +
                                                    <td>As this aminotransferase can produce hydrogen sulfide and CdS nanoparticles if S<sup>2-</sup>is in the system, special measures must be taken.</td>
 +
                                                  </tr>
 +
                                                  <tr>
 +
                                                    <td><a href="http://parts.igem.org/Part:BBa_K2242005">BBa_K2242005</a></td>
 +
                                                    <td>T7+LacO+KmAdh</td>
 +
                                                    <td>Coding</td>
 +
                                                    <td>948</td>
 +
                                                    <td>We use T7 promoter and Lac operator to control KMADH’s expression. Both of this two units are induced by IPTG. With this dual switch, we can reduce the leak of the gene expression as much as possible.</td>
 +
                                                    <td>The plasmid is bought from a bio-company. The Mtr gene is kindly provided by Prof.HongJiong. The recombinant plasmid is constructed by ourselves.</td>
 +
                                                    <td></td>
 +
                                                  </tr>
 +
                                                  <tr>
 +
                                                    <td><a href="http://parts.igem.org/Part:BBa_K2242384">BBa_K2242384</a></td>
 +
                                                    <td>placI+lacI+pTAC+KmAdh</td>
 +
                                                    <td>Coding</td>
 +
                                                    <td>2341</td>
 +
                                                    <td>To express this reductase KMADH in Shewallena, we construct a shuttle plasmid. On this plasmid, we use this pTAC to control its expression, which is induced by IPTG.</td>
 +
                                                    <td>The plasmid backbone pYYDT is kindly provided by Prof. Yu Hanqing. The gene KMADH is kindly provided by Prof. Hong Jiong. The recombinant plasmid is constructed by ourselves.</td>
 +
                                                    <td></td>
 +
                                                  </tr>
 +
                                              </table>
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Latest revision as of 14:43, 30 October 2017

子网页测试-队员


This year we, team USTC, submitted 7 basic parts. All of them are coding part as we did not use any special promoters or RBS in our project. These parts are different proteins that we have used in our project under control of different promoter or operator.

Part’s Number Part’s Name Type Length Function Source Safety Issues
BBa_K2242920 araC+pBAD+CmCR Coding 2089 To induce the gene expression, we use a promotor called pBAD, which is induced by arabinose. The plasmid is bought from a bio-company. The reductase CmCR’s gene is kindly provided by Prof.HongJiong. The recombinant plasmid is constructed by ourselves. The substrate of this reductase is COBE, which is an organic compound with a pungent smell. More importantly, it’s harmful to our skin and respiratory tract. So during the experiment process, we need to take up certain measures to protect ourselves.
BBa_K2242521 placI+lacI+pTAC+CmCR Coding 2326 We use a promoter pTAC to control the gene CmCR’s expression, which is induced by IPTG. The plasmid backbone pYYDT is kindly provided by Prof. Yu Hanqing. The gene CmCR is kindly provided by Prof. Hong Jiong. The recombinant plasmid is constructed by ourselves. The substrate of this reductase is COBE, which is an organic compound with a pungent smell. More importantly, it’s harmful to our skin and respiratory tract. So during the experiment process, we need to take up certain measures to protect ourselves.
BBa_K2242018 T7+LacO+Mtr Coding 5272 We use T7 promoter and Lac operator to control Mtr CAB’s expression. Both of this two units are induced by IPTG. With this dual switch, we can reduce the leak of the gene expression as much as possible. The plasmid is bought from a bio-company. The Mtr gene is obtained from the kit plate. The recombinant plasmid is constructed by ourselves.
BBa_K2242419 pTET+ccm Coding 6372 We use a constitutively on promoter pTET to control gene ccmA-H’s expression. The plasmid backbone and the promoter pTET is from the kit plate. The ccm A-H gene is amplified from the genome of an E.coli strain BL21(DE3). The recombinant plasmid is constructed by ourselves.
BBa_K2242633 pLuxR+CysDes Coding 2367 We use the pLuxR promoter from the quorum sensing system, which can be induced by AHL. The plasmid backbone and the promoter pLuxR is from the kit plate. The CysDes gene is synthesized by IDT. The recombinant plasmid is constructed by ourselves. As this aminotransferase can produce hydrogen sulfide and CdS nanoparticles if S2-is in the system, special measures must be taken.
BBa_K2242005 T7+LacO+KmAdh Coding 948 We use T7 promoter and Lac operator to control KMADH’s expression. Both of this two units are induced by IPTG. With this dual switch, we can reduce the leak of the gene expression as much as possible. The plasmid is bought from a bio-company. The Mtr gene is kindly provided by Prof.HongJiong. The recombinant plasmid is constructed by ourselves.
BBa_K2242384 placI+lacI+pTAC+KmAdh Coding 2341 To express this reductase KMADH in Shewallena, we construct a shuttle plasmid. On this plasmid, we use this pTAC to control its expression, which is induced by IPTG. The plasmid backbone pYYDT is kindly provided by Prof. Yu Hanqing. The gene KMADH is kindly provided by Prof. Hong Jiong. The recombinant plasmid is constructed by ourselves.






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