Difference between revisions of "Team:Stuttgart/Rose and Limonene Fragrance"

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             <p>HIER TEXT REINKOPIEREN
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             <p>The rose genes KDC-YjgB-ARO8 and ATF1 were sucessfully amplified by PCR, using plasmids pET28a-KDC-YjgB-ARO8 and pET28a-ATF1 from Guo et al.
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              The agarosegel analysis showed expected ties in the range between 4000-5000 kDa for KDC-YjgB-ARO8 and another tie at 1600 kDa for ATF1.
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              The arabiniose-inducable promotor pBAD (BBa_K206000 from the iGEM kit plate) could also be amplified by PCR. The purified PCR product showed a discrete tie at 130 kDa.
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              Additionally another PCR was perfomed, to couple the pBAD promotor to the gene complex with KDC-YjgB-ARO8.
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              The Verification by agarosegel electrophoresis showed that the coupling wasn't sucessfull.
 
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   <h8>Figure 9: </h8>
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   <h8>Figure 9: Agaraose-gel electrophoresis of purified rose-PCR products</h8>
 
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Revision as of 17:23, 30 October 2017

Results

Rose and Limonene Fragrance


Rose Fragrance

The rose genes KDC-YjgB-ARO8 and ATF1 were sucessfully amplified by PCR, using plasmids pET28a-KDC-YjgB-ARO8 and pET28a-ATF1 from Guo et al. The agarosegel analysis showed expected ties in the range between 4000-5000 kDa for KDC-YjgB-ARO8 and another tie at 1600 kDa for ATF1. The arabiniose-inducable promotor pBAD (BBa_K206000 from the iGEM kit plate) could also be amplified by PCR. The purified PCR product showed a discrete tie at 130 kDa. Additionally another PCR was perfomed, to couple the pBAD promotor to the gene complex with KDC-YjgB-ARO8. The Verification by agarosegel electrophoresis showed that the coupling wasn't sucessfull.

Figure 9: Agaraose-gel electrophoresis of purified rose-PCR products

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Figure 10: HIER TEXT REINKOPIEREN

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Figure 11:HIER TEXT REINKOPIEREN

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