Difference between revisions of "Team:Stuttgart/Rose and Limonene Fragrance"

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<h3>Esterases and Lipases</h3>
 
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<h4>Enzyme activity assay: Esterases</h4>
 
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            <p>In the following figures enzyme activities of the supernatant of the esterases EstCS2 and LIpB are shown. All results were obtained from biological triplicates. The detailed method for the activity determination is shown here: LINK
 
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                At the N-terminal end of the esterase EstCS2 a signal peptide (PelB) is added. Therefore the gene induction leads to enzyme expression and the expression of the signalpeptide and the secretion of the enzyme is enabled.
 
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<h1 align=middle> Results </h1>
 
<h1 align=middle> Results </h1>
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<h8>Figure 10: Agaraose-gel electrophoresis of purified rose-PCR products</h8>
 
 
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   <h4>Rose Fragrance</h4>
 
   <h4>Rose Fragrance</h4>
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             <p>The plasmids from Guo et al. pET28a-KDC-YjgB-ARO8 and pET28a-ATF1, used in this study, were sucessfully confirmed by sequencing the plasmids at GATC (Figure 9).
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             <p>The plasmids from Guo et al. pET28a-KDC-YjgB-ARO8 and pET28a-ATF1, used in this study, were sucessfully confirmed by sequencing the plasmids at GATC (Figure 9 and 10).
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               The rose genes KDC-YjgB-ARO8 and ATF1 were sucessfully amplified by PCR.
 
               The rose genes KDC-YjgB-ARO8 and ATF1 were sucessfully amplified by PCR.
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               The agarosegel analysis (Fig.10) showed expected ties in the range between 4000-5000 kDa for KDC-YjgB-ARO8 and another tie at 1600 kDa for ATF1.
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               The agarosegel analysis (Fig.11) showed expected ties in the range between 4000-5000 kDa for KDC-YjgB-ARO8 and another tie at 1600 kDa for ATF1.
 
               The arabiniose-inducable promotor pBAD (BBa_K206000 from the iGEM kit plate) could also be amplified by PCR. The purified PCR product showed a discrete tie at 130 kDa.
 
               The arabiniose-inducable promotor pBAD (BBa_K206000 from the iGEM kit plate) could also be amplified by PCR. The purified PCR product showed a discrete tie at 130 kDa.
 
               Additionally another PCR was perfomed, to couple the pBAD promotor to the gene complex with KDC-YjgB-ARO8.
 
               Additionally another PCR was perfomed, to couple the pBAD promotor to the gene complex with KDC-YjgB-ARO8.
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   <h8>Figure 10: Agaraose-gel electrophoresis of purified rose-PCR products</h8>
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   <h8>Figure 11: Agaraose-gel electrophoresis of purified rose-PCR products</h8>
 
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   <p>HIER TEXT REINKOPIEREN</p>
 
   <p>HIER TEXT REINKOPIEREN</p>
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             <p>HIER TEXT REINKOPIEREN</P>
 
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Revision as of 19:07, 30 October 2017

Results

Rose and Limonene Fragrance


Rose Fragrance

The plasmids from Guo et al. pET28a-KDC-YjgB-ARO8 and pET28a-ATF1, used in this study, were sucessfully confirmed by sequencing the plasmids at GATC (Figure 9 and 10).

The rose genes KDC-YjgB-ARO8 and ATF1 were sucessfully amplified by PCR. The agarosegel analysis (Fig.11) showed expected ties in the range between 4000-5000 kDa for KDC-YjgB-ARO8 and another tie at 1600 kDa for ATF1. The arabiniose-inducable promotor pBAD (BBa_K206000 from the iGEM kit plate) could also be amplified by PCR. The purified PCR product showed a discrete tie at 130 kDa. Additionally another PCR was perfomed, to couple the pBAD promotor to the gene complex with KDC-YjgB-ARO8. The Verification by agarosegel electrophoresis showed that the coupling wasn't sucessfull.

Figure 11: Agaraose-gel electrophoresis of purified rose-PCR products

HIER TEXT REINKOPIEREN

Figure 10: HIER TEXT REINKOPIEREN

HIER TEXT REINKOPIEREN

Figure 11:HIER TEXT REINKOPIEREN

HIER TEXT REINKOPIEREN