Difference between revisions of "Team:Stuttgart/Rose and Limonene Fragrance"
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| + | <h8>Figure 9: Sequenced rose plasmid (pET28a-ATF1) from Guo et al. </h8> | ||
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| + | <h8>Figure 10: Sequenced rose plasmid (pET28a-KDC-YjgB-ARO8) from Guo et al. </h8> | ||
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Revision as of 19:12, 30 October 2017
Results
Rose and Limonene Fragrance
Rose Fragrance
The plasmids from Guo et al. pET28a-KDC-YjgB-ARO8 and pET28a-ATF1, used in this study, were sucessfully confirmed by sequencing the plasmids at GATC (Figure 9 and 10).
The rose genes KDC-YjgB-ARO8 and ATF1 were sucessfully amplified by PCR. The agarosegel analysis (Fig.11) showed expected ties in the range between 4000-5000 kDa for KDC-YjgB-ARO8 and another tie at 1600 kDa for ATF1. The arabiniose-inducable promotor pBAD (BBa_K206000 from the iGEM kit plate) could also be amplified by PCR. The purified PCR product showed a discrete tie at 130 kDa. Additionally another PCR was perfomed, to couple the pBAD promotor to the gene complex with KDC-YjgB-ARO8. The Verification by agarosegel electrophoresis showed that the coupling wasn't sucessfull.
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