Difference between revisions of "Team:Stuttgart/Rose and Limonene Fragrance"
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<p>The plasmids from Guo et al. pET28a-KDC-YjgB-ARO8 and pET28a-ATF1, used in this study, were sucessfully confirmed by sequencing the plasmids at GATC (Figure 9 and 10). | <p>The plasmids from Guo et al. pET28a-KDC-YjgB-ARO8 and pET28a-ATF1, used in this study, were sucessfully confirmed by sequencing the plasmids at GATC (Figure 9 and 10). | ||
| + | The rose genes KDC-YjgB-ARO8 and ATF1 were sucessfully amplified by PCR. | ||
| + | The agarosegel analysis (Fig.11) showed expected ties in the range between 4000-5000 kDa for KDC-YjgB-ARO8 and another tie at 1600 kDa for ATF1. | ||
| + | The arabiniose-inducable promotor pBAD (BBa_K206000 from the iGEM kit plate) could also be amplified by PCR. The purified PCR product showed a discrete tie at 130 kDa. | ||
| + | Additionally another PCR was perfomed, to couple the pBAD promotor to the gene complex with KDC-YjgB-ARO8. | ||
| + | The Verification by agarosegel electrophoresis showed that the coupling wasn't sucessfull. | ||
</p> | </p> | ||
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<h8>Figure 9: Sequenced rose plasmid (pET28a-ATF1) from Guo et al. </h8> | <h8>Figure 9: Sequenced rose plasmid (pET28a-ATF1) from Guo et al. </h8> | ||
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<!--<https://static.igem.org/mediawiki/2017/d/d7/Rosescent.png">--> | <!--<https://static.igem.org/mediawiki/2017/d/d7/Rosescent.png">--> | ||
<img src="https://static.igem.org/mediawiki/2017/7/77/PETKDCYjgBAro8.png" class="img-responsive"/> | <img src="https://static.igem.org/mediawiki/2017/7/77/PETKDCYjgBAro8.png" class="img-responsive"/> | ||
<h8>Figure 10: Sequenced rose plasmid (pET28a-KDC-YjgB-ARO8) from Guo et al. </h8> | <h8>Figure 10: Sequenced rose plasmid (pET28a-KDC-YjgB-ARO8) from Guo et al. </h8> | ||
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| + | <!--<https://static.igem.org/mediawiki/2017/d/d1/Gelrose1.png">--> | ||
| + | <img src="https://static.igem.org/mediawiki/2017/f/fa/Rosegel.png" class="img-responsive"/> | ||
| + | <h8>Figure 11: Agaraose-gel electrophoresis of purified rose-PCR products</h8> | ||
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<p>HIER TEXT REINKOPIEREN</p> | <p>HIER TEXT REINKOPIEREN</p> | ||
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Revision as of 19:19, 30 October 2017
Results
Rose and Limonene Fragrance
Rose Fragrance
The plasmids from Guo et al. pET28a-KDC-YjgB-ARO8 and pET28a-ATF1, used in this study, were sucessfully confirmed by sequencing the plasmids at GATC (Figure 9 and 10). The rose genes KDC-YjgB-ARO8 and ATF1 were sucessfully amplified by PCR. The agarosegel analysis (Fig.11) showed expected ties in the range between 4000-5000 kDa for KDC-YjgB-ARO8 and another tie at 1600 kDa for ATF1. The arabiniose-inducable promotor pBAD (BBa_K206000 from the iGEM kit plate) could also be amplified by PCR. The purified PCR product showed a discrete tie at 130 kDa. Additionally another PCR was perfomed, to couple the pBAD promotor to the gene complex with KDC-YjgB-ARO8. The Verification by agarosegel electrophoresis showed that the coupling wasn't sucessfull.
HIER TEXT REINKOPIEREN
HIER TEXT REINKOPIEREN
HIER TEXT REINKOPIEREN
