Difference between revisions of "Team:Stuttgart/Keratinases"
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<h8>Figure 6: </h8> | <h8>Figure 6: </h8> | ||
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| − | <div class="col-xs-5 col-sm-4 col-md-4"> | + | <div class="col-xs-5 col-sm-4 col-md-4"><br> |
| − | <p> | + | <p>TEXT</p><br> |
| + | |||
</div> | </div> | ||
</div> | </div> | ||
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<!--<https://static.igem.org/mediawiki/2017/a/a0/EsteraseResults2.png">--> | <!--<https://static.igem.org/mediawiki/2017/a/a0/EsteraseResults2.png">--> | ||
<img src="" class="img-responsive"/> | <img src="" class="img-responsive"/> | ||
| − | <h8>Figure 7: | + | <h8>Figure 7: cell disruption (dilution 1:25)</h8><br> |
| − | + | <table> <tr> <th>sample</th> <th>OD562</th><th>concentration [µg/mL]</th> </tr> | |
| + | <tr><td>KerP psD1K3</td><td>0,514</td><td>11075,000</td></tr> | ||
| + | <tr><td>KerP psD1C3</td><td>0,611</td><td>13491,667</td></tr> | ||
| + | <tr><td>KerUS pet28</td><td>0,405</td><td>8358,333</td></tr> | ||
| + | <tr><td>KerUS psD1C3</td><td>0,285</td><td>5350,000</td></tr> | ||
| + | <tr><td>KerUS BB</td><td>0,385</td><td>7841,667</td></tr> | ||
| + | <tr><td>KerA pet28</td><td>0,482</td><td>10266,667</td></tr> | ||
| + | <tr><td>KerA psB1C3</td>0,585<td></td><td>12858,333</td></tr> | ||
| + | <tr><td>KerA BB</td><td>0,410</td><td>8475,000</td></tr> | ||
| + | <tr><td>wild type <i>E.coli</i></td><td>0,615</td><td>13600,000</td></tr> | ||
| + | </table> | ||
</div> | </div> | ||
<div class="col-xs-12 col-sm-4 col-md-4"> | <div class="col-xs-12 col-sm-4 col-md-4"> | ||
| − | <p> | + | <p>BCA Assay Results</p> |
| + | <br>Protein concentration of supernatant and cell disruption of <i>E.coli</i> carrying different keratinase plasmids (KerP, KerUS and KerA) was measured with BCA Assay method (see chapter methods). | ||
| + | The picture below shows the BSA standard curve (blank corrected, arithmetic average of triplicates):<br> | ||
| + | <img src="https://static.igem.org/mediawiki/2017/7/75/Stgt_bcastd.png"/> <br></P> | ||
</div> | </div> | ||
</div> | </div> | ||
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<!--<https://2017.igem.org/File:EsteraseResults3.png">--> | <!--<https://2017.igem.org/File:EsteraseResults3.png">--> | ||
<img src="" class="img-responsive"/> | <img src="" class="img-responsive"/> | ||
| − | <h8>Figure 8: | + | <h8>Figure 8: supernatant (dilution 1:25)</h8><br> |
| + | <table> <tr> <th>sample</th> <th>OD562</th><th>concentration [µg/mL]</th> </tr> | ||
| + | <tr><td>KerP psD1K3</td><td>0,194</td><td>3066,667</td></tr> | ||
| + | <tr><td>KerP psD1C3</td><td>0,195</td><td>3091,667</td></tr> | ||
| + | <tr><td>KerUS pet28</td><td>0,191</td><td>2991,667</td></tr> | ||
| + | <tr><td>KerUS psD1C3</td><td>0,207</td><td>3391,667</td></tr> | ||
| + | <tr><td>KerUS BB</td><td>0,226</td><td>3866,667</td></tr> | ||
| + | <tr><td>KerA pet28</td><td>0,194</td><td>3083,333</td></tr> | ||
| + | <tr><td>KerA psB1C3</td><td>0,213</td><td>3550,000</td></tr> | ||
| + | <tr><td>KerA BB</td><td>0,201</td><td>3241,667</td></tr> | ||
| + | <tr><td>wild type <i>E.coli</i></td><td>0,248</td><td>4416,667</td></tr> | ||
| + | </table> | ||
</div> | </div> | ||
<div class="col-xs-12 col-sm-4 col-md-4"> | <div class="col-xs-12 col-sm-4 col-md-4"> | ||
Revision as of 22:01, 30 October 2017
Results
Keratinases
HIER TEXT REINKOPIEREN
TEXT
| sample | OD562 | concentration [µg/mL] |
|---|---|---|
| KerP psD1K3 | 0,514 | 11075,000 |
| KerP psD1C3 | 0,611 | 13491,667 |
| KerUS pet28 | 0,405 | 8358,333 |
| KerUS psD1C3 | 0,285 | 5350,000 |
| KerUS BB | 0,385 | 7841,667 |
| KerA pet28 | 0,482 | 10266,667 |
| KerA psB1C3 | 0,58512858,333 | |
| KerA BB | 0,410 | 8475,000 |
| wild type E.coli | 0,615 | 13600,000 |
BCA Assay Results
Protein concentration of supernatant and cell disruption of E.coli carrying different keratinase plasmids (KerP, KerUS and KerA) was measured with BCA Assay method (see chapter methods). The picture below shows the BSA standard curve (blank corrected, arithmetic average of triplicates):
| sample | OD562 | concentration [µg/mL] |
|---|---|---|
| KerP psD1K3 | 0,194 | 3066,667 |
| KerP psD1C3 | 0,195 | 3091,667 |
| KerUS pet28 | 0,191 | 2991,667 |
| KerUS psD1C3 | 0,207 | 3391,667 |
| KerUS BB | 0,226 | 3866,667 |
| KerA pet28 | 0,194 | 3083,333 |
| KerA psB1C3 | 0,213 | 3550,000 |
| KerA BB | 0,201 | 3241,667 |
| wild type E.coli | 0,248 | 4416,667 |
HIER TEXT REINKOPIEREN
