Difference between revisions of "Team:Stuttgart/Keratinases"

Line 43: Line 43:
 
<tr><td>KerUS BB</td><td>0,385</td><td>7841,667</td></tr>
 
<tr><td>KerUS BB</td><td>0,385</td><td>7841,667</td></tr>
 
<tr><td>KerA pet28</td><td>0,482</td><td>10266,667</td></tr>
 
<tr><td>KerA pet28</td><td>0,482</td><td>10266,667</td></tr>
−
<tr><td>KerA psB1C3</td>0,585<td></td><td>12858,333</td></tr>
+
<tr><td>KerA psB1C3</td><td>0,585</td><td>12858,333</td></tr>
 
<tr><td>KerA BB</td><td>0,410</td><td>8475,000</td></tr>
 
<tr><td>KerA BB</td><td>0,410</td><td>8475,000</td></tr>
 
<tr><td>wild type <i>E.coli</i></td><td>0,615</td><td>13600,000</td></tr>
 
<tr><td>wild type <i>E.coli</i></td><td>0,615</td><td>13600,000</td></tr>

Revision as of 22:02, 30 October 2017

Results

Keratinases


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Figure 6:

TEXT


Figure 7: cell disruption (dilution 1:25)
sample OD562concentration [µg/mL]
KerP psD1K30,51411075,000
KerP psD1C30,61113491,667
KerUS pet280,4058358,333
KerUS psD1C30,2855350,000
KerUS BB0,3857841,667
KerA pet280,48210266,667
KerA psB1C30,58512858,333
KerA BB0,4108475,000
wild type E.coli0,61513600,000

BCA Assay Results


Protein concentration of supernatant and cell disruption of E.coli carrying different keratinase plasmids (KerP, KerUS and KerA) was measured with BCA Assay method (see chapter methods). The picture below shows the BSA standard curve (blank corrected, arithmetic average of triplicates):

Figure 8: supernatant (dilution 1:25)
sample OD562concentration [µg/mL]
KerP psD1K30,1943066,667
KerP psD1C30,1953091,667
KerUS pet280,1912991,667
KerUS psD1C30,2073391,667
KerUS BB0,2263866,667
KerA pet280,1943083,333
KerA psB1C30,2133550,000
KerA BB0,2013241,667
wild type E.coli0,2484416,667

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