Difference between revisions of "Team:Sydney Australia/Parts"

Line 649: Line 649:
  
 
</div>
 
</div>
−
</div>
 
−
</div>
 
−
</div>
 
−
 
−
<div class="row" style="height:10vw;"></div>
 
−
 
−
<div class = "row">
 
−
<div class = "content" id="please">
 
−
<center><h1>BASIC PARTS</h1>
 
−
<div class="box4"></div></center>
 
−
<div class="row" style="height:2vw;"></div>
 
−
<div style="text-align:center">
 
−
<table class="MsoTableGrid" border="1" cellspacing="0" cellpadding="0" width="100%" style="width:100.0%;border-collapse:collapse;border:none;mso-border-alt:solid windowtext .5pt; mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt">
 
−
<tbody><tr style="mso-yfti-irow:0;mso-yfti-firstrow:yes;height:18.4pt">
 
−
  <td width="114" valign="top" style="width:114.15pt;border:solid windowtext 1.0pt;
 
−
  mso-border-alt:solid windowtext .5pt;background:#D9E2F3;mso-background-themecolor:
 
−
  accent1;mso-background-themetint:51;padding:0cm 5.4pt 0cm 5.4pt;height:18.4pt">
 
−
  <p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-size:14.0pt;
 
−
  font-family:Quicksand"><br>
 
−
  PART NO.<o:p></o:p></span></b></p>
 
−
  <p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-size:14.0pt;
 
−
  font-family:Quicksand"><o:p>&nbsp;</o:p></span></b></p>
 
−
  </td>
 
−
  <td width="144" valign="top" style="width:143.85pt;border:solid windowtext 1.0pt;
 
−
  border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:
 
−
  solid windowtext .5pt;background:#D9E2F3;mso-background-themecolor:accent1;
 
−
  mso-background-themetint:51;padding:0cm 5.4pt 0cm 5.4pt;height:18.4pt">
 
−
  <p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-size:14.0pt;
 
−
  font-family:Quicksand"><br>
 
−
  PART NAME<o:p></o:p></span></b></p>
 
−
  </td>
 
−
  <td width="116" valign="top" style="width:115.95pt;border:solid windowtext 1.0pt;
 
−
  border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:
 
−
  solid windowtext .5pt;background:#D9E2F3;mso-background-themecolor:accent1;
 
−
  mso-background-themetint:51;padding:0cm 5.4pt 0cm 5.4pt;height:18.4pt">
 
−
  <p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-size:14.0pt;
 
−
  font-family:Quicksand"><br>
 
−
  SOURCE<o:p></o:p></span></b></p>
 
−
  </td>
 
−
  <td width="323" valign="top" style="width:322.55pt;border:solid windowtext 1.0pt;
 
−
  border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:
 
−
  solid windowtext .5pt;background:#D9E2F3;mso-background-themecolor:accent1;
 
−
  mso-background-themetint:51;padding:0cm 5.4pt 0cm 5.4pt;height:18.4pt">
 
−
  <p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:normal"><span lang="EN-US" style="font-size:14.0pt;
 
−
  font-family:Quicksand"><br>
 
−
  DESCRIPTION<o:p></o:p></span></b></p>
 
−
  </td>
 
−
</tr>
 
−
<tr style="mso-yfti-irow:1">
 
−
  <td width="114" valign="top" style="width:114.15pt;border:solid windowtext 1.0pt;
 
−
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 
−
  padding:0cm 5.4pt 0cm 5.4pt">
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417004">BBa_K2417004</a><o:p></o:p></span></p>
 
−
  </td>
 
−
  <td width="144" valign="top" style="width:143.85pt;border-top:none;border-left:
 
−
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt">
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Glycine Glycine Serine (x4) Linker Motif A<o:p></o:p></span></p>
 
−
  </td>
 
−
  <td width="116" valign="top" style="width:115.95pt;border-top:none;border-left:
 
−
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt">
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Designed<o:p></o:p></span></p>
 
−
  </td>
 
−
  <td width="323" valign="top" style="width:322.55pt;border-top:none;border-left:
 
−
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt">
 
−
  <p class="MsoListParagraphCxSpFirst" style="text-indent:-00.0pt;mso-list:l8 level1 lfo3"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Linker motif used
 
−
  to provide spacer region<o:p></o:p></span></p>
 
−
  <p class="MsoListParagraphCxSpMiddle" style="text-indent:-00.0pt;mso-list:l8 level1 lfo3"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Used to expose
 
−
  affinity purification tags such as 6x His tag<o:p></o:p></span></p>
 
−
  <p class="MsoListParagraphCxSpLast" style="text-indent:-00.0pt;mso-list:l8 level1 lfo3"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Serine and glycine
 
−
  used as they are small and flexible<o:p></o:p></span></p>
 
−
  </td>
 
−
</tr>
 
−
<tr style="mso-yfti-irow:2">
 
−
  <td width="114" valign="top" style="width:114.15pt;border:solid windowtext 1.0pt;
 
−
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 
−
  padding:0cm 5.4pt 0cm 5.4pt">
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417006">BBa_K2417006</a><o:p></o:p></span></p>
 
−
  </td>
 
−
  <td width="144" valign="top" style="width:143.85pt;border-top:none;border-left:
 
−
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt">
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Human Proinsulin Coding Sequence<o:p></o:p></span></p>
 
−
  </td>
 
−
  <td width="116" valign="top" style="width:115.95pt;border-top:none;border-left:
 
−
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt">
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Humans<o:p></o:p></span></p>
 
−
  </td>
 
−
  <td width="323" valign="top" style="width:322.55pt;border-top:none;border-left:
 
−
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt">
 
−
  <p class="MsoListParagraphCxSpFirst" style="text-indent:-00.0pt;mso-list:l1 level1 lfo4"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Coding sequence for
 
−
  human proinsulin, sourced from NCIB<o:p></o:p></span></p>
 
−
  <p class="MsoListParagraphCxSpMiddle" style="text-indent:-00.0pt;mso-list:l1 level1 lfo4"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Consists of A, B
 
−
  and C chains, but C-peptide is cleaved out<o:p></o:p></span></p>
 
−
  <p class="MsoListParagraphCxSpMiddle" style="text-indent:-00.0pt;mso-list:l1 level1 lfo4"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Contains 3 <span class="SpellE">disulphide</span> bonds<o:p></o:p></span></p>
 
−
  <p class="MsoListParagraphCxSpLast" style="text-indent:-00.0pt;mso-list:l1 level1 lfo4"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Contains arginine
 
−
  residues adjacent to C-peptide to allow cleavage<o:p></o:p></span></p>
 
−
  </td>
 
−
</tr>
 
−
<tr style="mso-yfti-irow:3">
 
−
  <td width="114" valign="top" style="width:114.15pt;border:solid windowtext 1.0pt;
 
−
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 
−
  padding:0cm 5.4pt 0cm 5.4pt">
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417007">BBa_K2417007</a><o:p></o:p></span></p>
 
−
  </td>
 
−
  <td width="144" valign="top" style="width:143.85pt;border-top:none;border-left:
 
−
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt">
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span class="SpellE"><span lang="EN-US" style="font-size:14.0pt;
 
−
  font-family:Quicksand">Winsulin - Single Chain Insulin Analogue<o:p></o:p></span></p>
 
−
  </td>
 
−
  <td width="116" valign="top" style="width:115.95pt;border-top:none;border-left:
 
−
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt">
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Designed<o:p></o:p></span></p>
 
−
  </td>
 
−
  <td width="323" valign="top" style="width:322.55pt;border-top:none;border-left:
 
−
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt">
 
−
  <p class="MsoListParagraphCxSpFirst" style="text-indent:-00.0pt;mso-list:l5 level1 lfo5"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Consists of short linker peptide instead of C-peptide<o:p></o:p></span></p>
 
−
  <p class="MsoListParagraphCxSpMiddle" style="text-indent:-00.0pt;mso-list:l5 level1 lfo5"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Does not require cleavage therefore simplifies production procedures<o:p></o:p></span></p>
 
−
  <p class="MsoListParagraphCxSpMiddle" style="text-indent:-00.0pt;mso-list:l5 level1 lfo5"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Linker peptide raises <span class="SpellE">pI</span> of insulin analogue (long lasting after administration)<o:p></o:p></span></p>
 
−
  <p class="MsoListParagraphCxSpLast" style="text-indent:-00.0pt;mso-list:l5 level1 lfo5"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Increased <span class="SpellE">thermostability</span> (based on other single chain insulin analogues tested)<o:p></o:p></span></p>
 
−
  </td>
 
−
</tr>
 
−
<tr style="mso-yfti-irow:4">
 
−
  <td width="114" valign="top" style="width:114.15pt;border:solid windowtext 1.0pt;
 
−
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 
−
  padding:0cm 5.4pt 0cm 5.4pt">
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417008">BBa_K2417008</a><o:p></o:p></span></p>
 
−
  </td>
 
−
  <td width="144" valign="top" style="width:143.85pt;border-top:none;border-left:
 
−
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt">
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"> 6x Poly-Histidine Tag codon optimized for use in <i style="mso-bidi-font-style:
 
−
  normal">E. coli</i><o:p></o:p></span></p>
 
−
  </td>
 
−
  <td width="116" valign="top" style="width:115.95pt;border-top:none;border-left:
 
−
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt">
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br> Designed (optimized for use in <i style="mso-bidi-font-style:normal">Escherichia coli</i>)<br style="mso-special-character:line-break">
 
−
  <!--[if !supportLineBreakNewLine]--><br style="mso-special-character:line-break">
 
−
  <!--[endif]--><o:p></o:p></span></p>
 
−
  </td>
 
−
  <td width="323" valign="top" style="width:322.55pt;border-top:none;border-left:
 
−
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt">
 
−
  <p class="MsoListParagraphCxSpFirst" style="text-indent:-00.0pt;mso-list:l6 level1 lfo6"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Codon optimized for use in <i style="mso-bidi-font-style:normal">E. coli</i><o:p></o:p></span></p>
 
−
  <p class="MsoListParagraphCxSpMiddle" style="text-indent:-00.0pt;mso-list:l6 level1 lfo6"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Used to immobilize ions on a chromatography affinity column (nickel, cobalt or copper)<o:p></o:p></span></p>
 
−
  <p class="MsoListParagraphCxSpLast" style="text-indent:-00.0pt;mso-list:l6 level1 lfo6"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Small, and easy to add to the ends of proteins of interest<o:p></o:p></span></p>
 
−
  </td>
 
−
</tr>
 
−
<tr style="mso-yfti-irow:5;height:27.45pt">
 
−
  <td width="114" valign="top" style="width:114.15pt;border:solid windowtext 1.0pt;
 
−
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 
−
  padding:0cm 5.4pt 0cm 5.4pt;height:27.45pt">
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br style="mso-special-character:line-break">
 
−
  <!--[if !supportLineBreakNewLine]--><br style="mso-special-character:line-break">
 
−
  <!--[endif]--><o:p></o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417009">BBa_K2417009</a><o:p></o:p></span></p>
 
−
  </td>
 
−
  <td width="144" valign="top" style="width:143.85pt;border-top:none;border-left:
 
−
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:27.45pt">
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br>Extended ribosome binding site<o:p></o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  </td>
 
−
  <td width="116" valign="top" style="width:115.95pt;border-top:none;border-left:
 
−
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:27.45pt">
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">T7 major capsid protein<o:p></o:p></span></p>
 
−
  </td>
 
−
  <td width="323" valign="top" style="width:322.55pt;border-top:none;border-left:
 
−
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:27.45pt">
 
−
  <p class="MsoListParagraphCxSpFirst" style="text-indent:-00.0pt;mso-list:l3 level1 lfo7"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">High ribosomal recruitment efficiency<o:p></o:p></span></p>
 
−
  <p class="MsoListParagraphCxSpLast" style="text-indent:-00.0pt;mso-list:l3 level1 lfo7"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Sourced from the pET15b vectors<o:p></o:p></span></p>
 
−
  </td>
 
−
</tr>
 
−
<tr style="mso-yfti-irow:6">
 
−
  <td width="114" valign="top" style="width:114.15pt;border:solid windowtext 1.0pt;
 
−
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 
−
  padding:0cm 5.4pt 0cm 5.4pt">
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417010">BBa_K2417010</a><o:p></o:p></span></p>
 
−
  </td>
 
−
  <td width="144" valign="top" style="width:143.85pt;border-top:none;border-left:
 
−
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt">
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span class="SpellE"><span lang="EN-US" style="font-size:14.0pt;
 
−
  font-family:Quicksand">Ecotin</span></span><span lang="EN-US" style="font-size:
 
−
  14.0pt;font-family:Quicksand"> Periplasmic Signal Peptide<o:p></o:p></span></p>
 
−
  </td>
 
−
  <td width="116" valign="top" style="width:115.95pt;border-top:none;border-left:
 
−
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt">
 
−
  <p class="MsoNormal"><i style="mso-bidi-font-style:normal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></i></p>
 
−
  <p class="MsoNormal"><i style="mso-bidi-font-style:normal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></i></p>
 
−
  <p class="MsoNormal"><i style="mso-bidi-font-style:normal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Escherichia coli<o:p></o:p></span></i></p>
 
−
  </td>
 
−
  <td width="323" valign="top" style="width:322.55pt;border-top:none;border-left:
 
−
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt">
 
−
  <p class="MsoListParagraphCxSpFirst" style="text-indent:-00.0pt;mso-list:l2 level1 lfo8"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Small periplasmic, <span class="SpellE">homodimeric</span> protein<o:p></o:p></span></p>
 
−
  <p class="MsoListParagraphCxSpMiddle" style="text-indent:-00.0pt;mso-list:l2 level1 lfo8"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Upon fusion to N-terminus of protein will translocate protein to periplasm of <i style="mso-bidi-font-style:normal">E. coli</i><o:p></o:p></span></p>
 
−
  <p class="MsoListParagraphCxSpMiddle" style="text-indent:-00.0pt;mso-list:l2 level1 lfo8"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Periplasm is an oxidative environment ideal for protein folding, and simplifies extraction due to eliminating the need for purifying inclusion bodies from the cytoplasm<o:p></o:p></span></p>
 
−
  <p class="MsoListParagraphCxSpLast" style="text-indent:-00.0pt;mso-list:l2 level1 lfo8"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Periplasm has reduced proteases<o:p></o:p></span></p>
 
−
  </td>
 
−
</tr>
 
−
<tr style="mso-yfti-irow:7">
 
−
  <td width="114" valign="top" style="width:114.15pt;border:solid windowtext 1.0pt;
 
−
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 
−
  padding:0cm 5.4pt 0cm 5.4pt">
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br style="mso-special-character:line-break">
 
−
  <!--[if !supportLineBreakNewLine]--><br style="mso-special-character:line-break">
 
−
  <!--[endif]--><o:p></o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417011">BBa_K2417011</a><o:p></o:p></span></p>
 
−
  </td>
 
−
  <td width="144" valign="top" style="width:143.85pt;border-top:none;border-left:
 
−
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt">
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span class="SpellE"><span lang="EN-US" style="font-size:14.0pt;
 
−
  font-family:Quicksand">YncM Bacillus Secretion Peptide<o:p></o:p></span></p>
 
−
  </td>
 
−
  <td width="116" valign="top" style="width:115.95pt;border-top:none;border-left:
 
−
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt">
 
−
  <p class="MsoNormal"><i style="mso-bidi-font-style:normal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></i></p>
 
−
  <p class="MsoNormal"><i style="mso-bidi-font-style:normal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></i></p>
 
−
  <p class="MsoNormal"><i style="mso-bidi-font-style:normal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Bacillus subtilis<o:p></o:p></span></i></p>
 
−
  </td>
 
−
  <td width="323" valign="top" style="width:322.55pt;border-top:none;border-left:
 
−
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt">
 
−
  <p class="MsoListParagraphCxSpFirst" style="text-indent:-00.0pt;mso-list:l0 level1 lfo9"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Can be fused to the
 
−
  N-terminus of proteins to induce secretion to the surrounding media wheN proteins are expressed in <i style="mso-bidi-font-style:normal">Bacillus subtilis</i><o:p></o:p></span></p>
 
−
  <p class="MsoListParagraphCxSpLast" style="text-indent:-00.0pt;mso-list:l0 level1 lfo9"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Eliminates many
 
−
  steps in the purification process<o:p></o:p></span></p>
 
−
  </td>
 
−
</tr>
 
−
<tr style="mso-yfti-irow:8;height:85.45pt">
 
−
  <td width="114" valign="top" style="width:114.15pt;border:solid windowtext 1.0pt;
 
−
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 
−
  padding:0cm 5.4pt 0cm 5.4pt;height:85.45pt">
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417012">BBa_K2417012<o:p></o:p></a></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  </td>
 
−
  <td width="144" valign="top" style="width:143.85pt;border-top:none;border-left:
 
−
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:85.45pt">
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">TEV Protease Cleavage Sequence<o:p></o:p></span></p>
 
−
  </td>
 
−
  <td width="116" valign="top" style="width:115.95pt;border-top:none;border-left:
 
−
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:85.45pt">
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N/A<o:p></o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  </td>
 
−
  <td width="323" valign="top" style="width:322.55pt;border-top:none;border-left:
 
−
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:85.45pt">
 
−
  <p class="MsoListParagraphCxSpFirst" style="text-indent:-00.0pt;mso-list:l4 level1 lfo10"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Sequence for which TEV protease is specific to<o:p></o:p></span></p>
 
−
  <p class="MsoListParagraphCxSpLast" style="text-indent:-00.0pt;mso-list:l4 level1 lfo10"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Can be added between protein of interest and peptides that require removal, such as purification tags<o:p></o:p></span></p>
 
−
  </td>
 
−
</tr>
 
−
<tr style="mso-yfti-irow:9;mso-yfti-lastrow:yes">
 
−
  <td width="114" valign="top" style="width:114.15pt;border:solid windowtext 1.0pt;
 
−
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 
−
  padding:0cm 5.4pt 0cm 5.4pt">
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417013">BBa_K2417013</a><o:p></o:p></span></p>
 
−
  </td>
 
−
  <td width="144" valign="top" style="width:143.85pt;border-top:none;border-left:
 
−
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt">
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Glycine
 
−
  <span class="SpellE">Glycine</span> Serine (x4) Linker <o:p></o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Motif
 
−
  B<o:p></o:p></span></p>
 
−
  </td>
 
−
  <td width="116" valign="top" style="width:115.95pt;border-top:none;border-left:
 
−
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt">
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
 
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Designed<o:p></o:p></span></p>
 
−
  </td>
 
−
  <td width="323" valign="top" style="width:322.55pt;border-top:none;border-left:
 
−
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt">
 
−
  <p class="MsoListParagraphCxSpFirst" style="text-indent:-00.0pt;mso-list:l8 level1 lfo3"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Linker motif used
 
−
  to provide spacer region<o:p></o:p></span></p>
 
−
  <p class="MsoListParagraphCxSpMiddle" style="text-indent:-00.0pt;mso-list:l8 level1 lfo3"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Used to expose
 
−
  affinity purification tags such as 6x His tag<o:p></o:p></span></p>
 
−
  <p class="MsoListParagraphCxSpLast" style="text-indent:-00.0pt;mso-list:l8 level1 lfo3"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
 
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Serine and glycine
 
−
  used as they are small and flexible<o:p></o:p></span></p>
 
−
  </td>
 
−
</tr>
 
−
</tbody>
 
−
</table>
 
 
</div>
 
</div>
 
</div>
 
</div>

Revision as of 02:00, 31 October 2017

COMPOSITE PARTS

All of our gold, silver and bronze winning parts are composite parts, consisting of a multitude of basic parts

The complete sequences and characteristics of these basic parts are linked on the Parts Registry pages of our composite parts which are linked in each table.

GOLD

 

PART NAME /
NO.

 


SOURCE


KEY FEATURES



WHY IS IT GOLD?











YncM-Winsulin

BBa_K2417005

 









Bacillus subtilis (YncM secretion tag)

 

 

Novel short chain insulin analogue (Winsulin) with a 12AA linker
(-QRGGGSGGGQRR-)

 

YncM secretion tag to translocate protein into media

 

N-terminal 6x His Tag purification

 

4x GSS flexible linker

 

TEV protease cleavage site

 

Extended ribosome binding site

 

 

 

We demonstrated that it works:

·      We tested it in insulin-sensitive cell lines and saw an increase in glycogen synthesis and glucose oxidation above basal levels, proving its bioactivity

·      We showed via an ELISA assay that whilst the cell lysate did not contain Winsulin, the media in which the cells were growing did. Therefore the YncM secretion tag worked as expected

 

The results for these assays can be found here











Ecotin-Proinsulin

BBa_K2417000

 









E. Coli (Ecotin tag)

 

 

 

Human proinsulin

 

N-terminal Ecotin tag for targeting proteins to the periplasm

 

N-terminal 6x His tag

 

4x GGS flexible linker

 

Trypsin protease cleavage site for His-tag and C-peptide cleavage

 

Extended ribosomal binding site

 



We improved previous parts:

BBa_M39904 and BBa_M1877 by:

 

Completing their sequence

 

Adding a periplasmic transporter tag (for more efficient folding in an oxidative environment)

 

Adding an efficient ribosomal binding site

 

Adding an N-terminal His tag for purification

 

We demonstrated that it works:

·      By testing it insulin-sensitive cell lines and saw an increase in glycogen synthesis and glucose oxidation above basal levels, therefore proving its bioactivity

 

The results for these assays can be found here







Human (Human proinsulin)

 

 

 

 




Cytoplasmic-Proinsulin     BBa_K2417001








Human (Human proinsulin)

Human proinsulin

 

N-terminal 6x His tag

 

4x GGS flexible linker

 

Trypsin protease cleavage site for His-tag and C-peptide cleavage

 

Extended ribosomal binding site

 

We improved previous parts:

BBa_M39904 and BBa_M1877 by:

·      Completing their sequence

·      Adding an efficient ribosomal binding site

·      Adding an N-terminal His tag for purification

 

We demonstrated that it works:

·      By testing it insulin-sensitive cell lines and saw an increase in glycogen synthesis and glucose oxidation above basal levels, therefore proving its bioactivity

 

The results for these assays can be found here

SILVER

 

PART NAME /
NO.

 



SOURCE



KEY FEATURES



WHY IS IT SILVER?

 

 

 

 

 

Cytoplasmic-Winsulin

BBa_K2417003

 



N/A – Winsulin is self designed from literature and mammalian insulins

 

 

Novel single chain insulin analogue (Winsulin) with a 12AA linker (QRGGGSGGGQRR)

 

N-terminal 6x His Tag

 

4x GSS flexible linker

 

TEV protease cleavage site

 

Extended ribosome binding site

 

 

 

·      We validated our parts by showing that they work in producing insulin. We performed an ELISA assay testing for properly folded insulin, and detected the presence of Cytoplasmic Winsulin.

 

 

The results for the assay can be found here

 

 

BRONZE

 

PART NAME /
NO.

 



SOURCE



KEY FEATURES



WHY IS IT BRONZE?

 

 

 

 

 

 

Ecotin-Winsulin

BBa_K2417002

 



E. Coli (Ecotin Tag)

 

 

Novel single chain insulin analogue (Winsulin) with a 12AA linker (QRGGGSGGGQRR)

 

N-terminal Ecotin tag for targeting proteins to the periplasm

 

N-terminal 6x His Tag

 

4x GSS flexible linker

 

TEV protease cleavage site

 

Extended ribosome binding site

 

 

 

 

 

 

 

We submitted this DNA part to the registry, for other teams to use in the future

 

N/A – Winsulin is self designed from literature and mammalian insulins