Difference between revisions of "Team:BIT-China/Notebook"

 
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{{BIT-China}}
 
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        <img src="https://static.igem.org/mediawiki/2017/e/ec/T--BIT-China--2017laboratory.jpg" alt="">
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    <h2 class="title-h2" id="mytop">NOTEBOOK</h2>
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    <h3 class="title-h3">Breakthrough</h3>
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    <h4 class="title-h4">2.14-4.14:team recruitment & brainstorm</h4>
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    <h4 class="title-h4">4.14-6.20: core part synthesis and heterologous expression</h4>
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      <p class="my-content-li2">4.14-5.2: core part-T1R2/T1R3 synthesis completely through OE-PCR.</p>
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      <p class="my-content-li2">4.30-6.20: heterologous expression of T1R2/T1R3. <a href="https://2017.igem.org/Team:BIT-China/Project/Sense">Click here to see more details.</a></p>
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    <h4 class="title-h4">4.30-5.15：modeling</h4>
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      <p class="my-content-li2">4.30-5.5: finished several sweeteners'docking as well as T1R2/T1R3 PDB file construction. Different sweeteners docked to the sweet taste receptor T1R2/T1R3.<a href="https://2017.igem.org/Team:BIT-China/Model/Docking">Click here to see more details.</a></p>
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      <p class="my-content-li2">5.5-5.15: MAPK pathway signal modeling. <a href="https://2017.igem.org/Team:BIT-China/Model/GPCRpathway">Click here to see more details.</a></p>
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    <h4 class="title-h4">5.2-8.20: pathway optimization</h4>
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      <p class="my-content-li2">5.2-7.1: single gene knockout</p>
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      <p class="my-content-li2">7.1-8.5: any two of the three genes knockout and three genes knockout<a href="https://2017.igem.org/Team:BIT-China/Project/Transduction">Click here to see more details.</a></p>
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      <p class="my-content-li2">8.5-8.20: characterization of engineered strains </p>
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    <h4 class="title-h4">8.21-10.20: Verification of the new pathway </h4>
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      <p class="my-content-li2">8.21-9.13: establishment of the detection circuit .<a href="https://2017.igem.org/Team:BIT-China/Project/Detection">Click here to see more details.</a></p>
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      <p class="my-content-li2">9.13-9.26: verification of the new pathway </p>
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      <p class="my-content-li2">9.27-10.10: sweetness meter verification</p>
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      <p class="my-content-li2">10.8-10.20: parts standardization</p>
  
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<h1>Notebook</h1>
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<p> Document the dates you worked on your project. This should be a detailed account of the work done each day for your project.</p>
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          No biggie... you can <a href="https://static.igem.org/mediawiki/2017/7/7c/Bit-china-notebook.pdf">click here to
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<p>You can see what others teams have done to organize their notes:</p>
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<li><a href="https://2014.igem.org/Team:ATOMS-Turkiye/Notebook">2014 ATOMS-Turkiye</a></li>
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<li><a href="https://2014.igem.org/Team:Tec-Monterrey/ITESM14_project.html#tab_notebook">2014 Tec Monterrey</a></li>
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<li><a href="https://2014.igem.org/Team:Kyoto/Notebook/Magnetosome_Formation#title">2014 Kyoto</a></li>
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<li><a href="https://2014.igem.org/Team:Cornell/notebook">2014 Cornell</a></li>
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            <li><a href="https://2017.igem.org/Team:BIT-China/Modeling">Modeling</a></li>
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            <li  class="active"><a href="https://2017.igem.org/Team:BIT-China/Notebook">Notebook</a></li>
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     <p class="my-content-p">For more details,please see our <a href="https://2017.igem.org/Team:BIT-China/Project/Description">project</a>.</p>
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    <span>TOP</span>
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{{BIT-China/footer}}

Latest revision as of 10:02, 31 October 2017

BIT-CHINA

NOTEBOOK

Breakthrough

2.14-4.14:team recruitment & brainstorm

4.14-6.20: core part synthesis and heterologous expression

4.14-5.2: core part-T1R2/T1R3 synthesis completely through OE-PCR.

4.30-6.20: heterologous expression of T1R2/T1R3. Click here to see more details.

4.30-5.15：modeling

4.30-5.5: finished several sweeteners'docking as well as T1R2/T1R3 PDB file construction. Different sweeteners docked to the sweet taste receptor T1R2/T1R3.Click here to see more details.

5.5-5.15: MAPK pathway signal modeling. Click here to see more details.

5.2-8.20: pathway optimization

5.2-7.1: single gene knockout

7.1-8.5: any two of the three genes knockout and three genes knockoutClick here to see more details.

8.5-8.20: characterization of engineered strains

8.21-10.20: Verification of the new pathway

8.21-9.13: establishment of the detection circuit .Click here to see more details.

9.13-9.26: verification of the new pathway

9.27-10.10: sweetness meter verification

10.8-10.20: parts standardization

It appears you don't have a PDF plugin for this browser. No biggie... you can click here to download the PDF file.

For more details,please see our project.

TOP

Contact

Institute of Biotransformation and synthetic biosystem

School of life science

Beijing Institute of Technology

100081, Beijing

Email: lichun@bit.edu.cn