<h4 class="title-h4">4.14-6.20: core part synthesis and heterologous expression</h4>
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<p class="my-content-li2">4.14-5.2: core part-T1R2/T1R3 synthesis completely through OE-PCR.</p>
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<p class="my-content-li2">4.30-6.20: heterologous expression of T1R2/T1R3. <a href="https://2017.igem.org/Team:BIT-China/Project/Sense">Click here to see more details.</a></p>
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<h4 class="title-h4">4.30-5.15:modeling</h4>
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<p class="my-content-li2">4.30-5.5: finished several sweeteners'docking as well as T1R2/T1R3 PDB file construction. Different sweeteners docked to the sweet taste receptor T1R2/T1R3.<a href="https://2017.igem.org/Team:BIT-China/Model/Docking">Click here to see more details.</a></p>
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<p class="my-content-li2">5.5-5.15: MAPK pathway signal modeling. <a href="https://2017.igem.org/Team:BIT-China/Model/GPCRpathway">Click here to see more details.</a></p>
<p class="my-content-li2">5.2-7.1: single gene knockout</p>
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<p class="my-content-li2">7.1-8.5: any two of the three genes knockout and three genes knockout<a href="https://2017.igem.org/Team:BIT-China/Project/Transduction">Click here to see more details.</a></p>
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<p class="my-content-li2">8.5-8.20: characterization of engineered strains </p>
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<h4 class="title-h4">8.21-10.20: Verification of the new pathway </h4>
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<p class="my-content-li2">8.21-9.13: establishment of the detection circuit .<a href="https://2017.igem.org/Team:BIT-China/Project/Detection">Click here to see more details.</a></p>
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<p class="my-content-li2">9.13-9.26: verification of the new pathway </p>
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<p class="my-content-li2">9.27-10.10: sweetness meter verification</p>
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<p class="my-content-li2">10.8-10.20: parts standardization</p>
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<p class="my-content-p">For more details,please see our <a href="https://2017.igem.org/Team:BIT-China/Project/Description">project</a>.</p>
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4.30-5.5: finished several sweeteners'docking as well as T1R2/T1R3 PDB file construction. Different sweeteners docked to the sweet taste receptor T1R2/T1R3.Click here to see more details.