Difference between revisions of "Team:Sydney Australia/Parts"

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<div class = "center">
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    <img class="center-block img-center" src="https://static.igem.org/mediawiki/2017/4/48/T--Sydney_Australia--partspage_banner.png" width="70%">
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    <img src="https://static.igem.org/mediawiki/2017/e/ee/T--Sydney_Australia--CompositePartsBanner.gif" width="70%">
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</div>
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</div>
 
</div>
  
 
<div class ="row" style="height:5vw;"></div>
 
<div class ="row" style="height:5vw;"></div>
  
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<div class = "row">
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<div class = "row"><groupparts></groupparts>
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<div class = "content" id="please" width="80%">
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<center><h1>COMPOSITE PARTS</h1></center>
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<center><div class="box5"></div></center>
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<center><h4>All of our gold, silver and bronze winning parts are composite parts, consisting of a multitude of basic parts<br><br> The complete sequences and characteristics of these basic parts are linked on the Parts Registry pages of our composite parts which are <b>linked in each table.</b></h4></center>
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</div>
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</div>
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<div class = "row">
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<div class = "content" id="please">
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<center><h1>GOLD</h1></center>
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<center><div class="box1"></div></center>
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<div class="row" style="height:2vw;"></div>
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<table class="MsoTableGrid" border="1" cellspacing="0" cellpadding="0" width="100%" style="width:100.0%;border-collapse:collapse;border:none;mso-border-alt:solid windowtext .5pt;
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mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt">
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<tbody><tr style="mso-yfti-irow:0;mso-yfti-firstrow:yes;height:19.05pt">
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  <td width="15%" style="width:15.92%;border:solid windowtext 1.0pt;mso-border-alt:
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  solid windowtext .5pt;background:#F6D562;padding:0cm 5.4pt 0cm 5.4pt;
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  height:19.05pt">
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">PART NAME /<br> NO.<o:p></o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  </td>
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  <td width="12%" style="width:12.88%;border:solid windowtext 1.0pt;border-left:
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  none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
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  background:#F6D562;padding:0cm 5.4pt 0cm 5.4pt;height:19.05pt">
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br>SOURCE<o:p></o:p></span></p>
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  </td>
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  <td width="29%" style="width:29.62%;border:solid windowtext 1.0pt;border-left:
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  none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
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  background:#F6D562;padding:0cm 5.4pt 0cm 5.4pt;height:19.05pt">
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br>KEY FEATURES<o:p></o:p></span></p>
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  </td>
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  <td width="41%" style="width:41.56%;border:solid windowtext 1.0pt;border-left:
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  none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
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  background:#F6D562;padding:0cm 5.4pt 0cm 5.4pt;height:19.05pt">
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br><br>WHY IS IT GOLD?<o:p></o:p></span></p>
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  </td>
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</tr>
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<tr style="mso-yfti-irow:1;height:78.9pt">
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  <td width="15%" style="width:15.92%;border:solid windowtext 1.0pt;border-top:
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  none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
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  padding:0cm 5.4pt 0cm 5.4pt;height:78.9pt">
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  <p class="MsoNormal" align="center" style="text-align:center"><span class="SpellE"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br><br><br><br><br><br><br><br><br><br>YncM-Winsulin</span></span><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p></o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417005">BBa_K2417005</a><o:p></o:p></span></p>
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  </td>
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  <td width="12%" style="width:12.88%;border-top:none;border-left:none;
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  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
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  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
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  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:78.9pt">
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br><br><br><br><br><br><br><br>Bacillus subtilis (<span class="SpellE">YncM</span> secretion tag)<o:p></o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  </td>
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  <td width="29%" style="width:29.62%;border-top:none;border-left:none;
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  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
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  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
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  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:78.9pt">
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Novel short chain insulin
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  analogue (<span class="SpellE">Winsulin</span>) with a 12AA linker <br>
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  (-QRGGGSGGGQRR-)<o:p></o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span class="SpellE"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">YncM</span></span><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"> secretion tag to
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  translocate protein into media<o:p></o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">N-terminal 6x His Tag
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  purification<o:p></o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">4x GSS flexible linker<o:p></o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">TEV protease cleavage site<o:p></o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Extended ribosome binding site<o:p></o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  </td>
+
−
  <td width="41%" valign="top" style="width:41.56%;border-top:none;border-left:
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  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
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−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:78.9pt">
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−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We
+
−
  demonstrated that it works:<o:p></o:p></span></p>
+
−
  <p class="MsoListParagraphCxSpFirst" style="text-indent:-00.0pt;mso-list:l10 level1 lfo19"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
+
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  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We tested it in
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  insulin-sensitive cell lines and saw an increase in glycogen synthesis and
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  glucose oxidation above basal levels, proving its bioactivity<br style="mso-special-character:line-break">
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  <!--[if !supportLineBreakNewLine]--><br style="mso-special-character:line-break">
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  <!--[endif]--><o:p></o:p></span></p>
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  <p class="MsoListParagraphCxSpMiddle" style="text-indent:-00.0pt;mso-list:l10 level1 lfo19"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
+
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We showed via an
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  ELISA assay that whilst the cell lysate did not contain <span class="SpellE">Winsulin</span>,
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  the media in which the cells were growing did. <span class="GramE">Therefore</span>
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  the <span class="SpellE">YncM</span> secretion tag worked as expected<o:p></o:p></span></p>
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  <p class="MsoListParagraphCxSpLast"><span lang="EN-US" style="font-size:15.0pt;
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  font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">The
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  results for these assays can be found <a href="https://2017.igem.org/Team:Sydney_Australia/Results">here</a><o:p></o:p></span></p>
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  </td>
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</tr>
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<tr style="mso-yfti-irow:2;height:206.35pt">
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  <td width="15%" rowspan="2" style="width:15.92%;border:solid windowtext 1.0pt;
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  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
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  padding:0cm 5.4pt 0cm 5.4pt;height:206.35pt">
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  <p class="MsoNormal" align="center" style="text-align:center"><span class="SpellE"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br><br><br><br><br><br><br><br><br><br>Ecotin</span></span><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">-Proinsulin<o:p></o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417000">BBa_K2417000<o:p></o:p></a></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  </td>
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  <td width="12%" style="width:12.88%;border-top:none;border-left:none;
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  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
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  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
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  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:206.35pt">
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br><br><br><br><br><br><br><br>E. Coli (<span class="SpellE">Ecotin</span>
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  tag)<o:p></o:p></span></p>
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  </td>
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  <td width="29%" rowspan="2" style="width:29.62%;border-top:none;border-left:
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  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
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  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
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  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:206.35pt">
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  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Human proinsulin<o:p></o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">N-terminal <span class="SpellE">Ecotin</span>
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  tag for targeting proteins to the periplasm<o:p></o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">N-terminal 6x His tag<o:p></o:p></span></p>
+
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">4x GGS flexible linker<o:p></o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Trypsin protease cleavage site
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  for His-tag and C-peptide cleavage<o:p></o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Extended ribosomal binding
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  site<o:p></o:p></span></p>
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  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  </td>
+
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  <td width="41%" rowspan="2" valign="top" style="width:41.56%;border-top:none;
+
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  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
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  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
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  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:206.35pt">
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  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br style="mso-special-character:line-break">
+
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  <!--[if !supportLineBreakNewLine]--><br style="mso-special-character:line-break">
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  <!--[endif]--><o:p></o:p></span></p>
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  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We
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  improved previous parts:<o:p></o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_M39904">BBa_M39904</a> and <a href="http://parts.igem.org/Part:BBa_M1877">BBa_M1877</a> by:<o:p></o:p></span></p>
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  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Completing their sequence<o:p></o:p></span></p>
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  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Adding a periplasmic
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  transporter tag (for more efficient folding in an oxidative environment)<o:p></o:p></span></p>
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  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Adding an efficient ribosomal binding
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  site<o:p></o:p></span></p>
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  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Adding an N-terminal His tag
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  for purification<o:p></o:p></span></p>
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
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  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We
+
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  demonstrated that it works:<o:p></o:p></span></p>
+
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  <p class="MsoListParagraph" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
+
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">By testing it
+
−
  insulin-sensitive cell lines and saw an increase in glycogen synthesis and
+
−
  glucose oxidation above basal levels, therefore proving its bioactivity<o:p></o:p></span></p>
+
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">The
+
−
  results for these assays can be found <a href="https://2017.igem.org/Team:Sydney_Australia/Results">here</a><o:p></o:p></span></p>
+
−
  </td>
+
−
</tr>
+
−
<tr style="mso-yfti-irow:3;height:72.85pt">
+
−
  <td width="12%" style="width:12.88%;border-top:none;border-left:none;
+
−
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:72.85pt">
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br><br><br><br><br><br>Human (<span class="SpellE">Human</span>
+
−
  proinsulin)<o:p></o:p></span></p>
+
−
  </td>
+
−
</tr>
+
−
<tr style="mso-yfti-irow:4;mso-yfti-lastrow:yes;height:42.15pt">
+
−
  <td width="15%" style="width:15.92%;border:solid windowtext 1.0pt;border-top:
+
−
  none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+
−
  padding:0cm 5.4pt 0cm 5.4pt;height:42.15pt">
+
−
    <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
      <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
        <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
          <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br><br><br<br><br>Cytoplasmic-Proinsulin <span style="mso-spacerun:yes">&nbsp;&nbsp;&nbsp;&nbsp;</span><a href="http://parts.igem.org/Part:BBa_K2417001">BBa_K2417001</a><o:p></o:p></span></p>
+
−
  </td>
+
−
  <td width="12%" style="width:12.88%;border-top:none;border-left:none;
+
−
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:42.15pt">
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><br><br><br><br><br><br><br>Human (<span class="SpellE">Human</span>
+
−
  proinsulin)<o:p></o:p></span></p>
+
−
  </td>
+
−
  <td width="29%" style="width:29.62%;border-top:none;border-left:none;
+
−
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:42.15pt">
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Human proinsulin<o:p></o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">N-terminal 6x His tag<o:p></o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">4x GGS flexible linker<o:p></o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Trypsin protease cleavage site
+
−
  for His-tag and C-peptide cleavage<o:p></o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Extended ribosomal binding
+
−
  site<o:p></o:p></span></p>
+
−
  </td>
+
−
  <td width="41%" valign="top" style="width:41.56%;border-top:none;border-left:
+
−
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:42.15pt">
+
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">We
+
−
  improved previous parts:<o:p></o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_M39904">BBa_M39904</a> and <a href="http://parts.igem.org/Part:BBa_M1877">BBa_M1877</a> by:<o:p></o:p></span></p>
+
−
  <p class="MsoListParagraphCxSpFirst" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
+
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Completing their
+
−
  sequence<o:p></o:p></span></p>
+
−
  <p class="MsoListParagraphCxSpMiddle" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
+
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Adding an efficient
+
−
  ribosomal binding site<o:p></o:p></span></p>
+
−
  <p class="MsoListParagraphCxSpMiddle" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
+
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">Adding an
+
−
  N-terminal His tag for purification<o:p></o:p></span></p>
+
−
  <p class="MsoListParagraphCxSpMiddle"><span lang="EN-US" style="font-size:15.0pt;
+
−
  font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoListParagraphCxSpMiddle"><span lang="EN-US" style="font-size:15.0pt;
+
−
  font-family:Quicksand">We demonstrated that it works:<o:p></o:p></span></p>
+
−
  <p class="MsoListParagraphCxSpLast" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:15.0pt;font-family:Symbol;mso-fareast-font-family:
+
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">By testing it insulin-sensitive
+
−
  cell lines and saw an increase in glycogen synthesis and glucose oxidation
+
−
  above basal levels, therefore proving its bioactivity<o:p></o:p></span></p>
+
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">The
+
−
  results for these assays can be found <a href="https://2017.igem.org/Team:Sydney_Australia/Results">here</a><o:p></o:p></span></p>
+
−
  </td>
+
−
</tr>
+
−
</tbody></table>
+
−
</div>
+
−
</div>
+
−
 
+
−
<div class="row" style="height:5vw;"></div>
+
−
 
+
−
<div class = "row">
+
−
<div class = "content" id="please">
+
−
<center><h1>SILVER</h1>
+
−
<div class="box2"></div></center>
+
−
<div class="row" style="height:2vw;"></div>
+
−
<div align="center">
+
−
<table class="MsoTableGrid" border="1" cellspacing="0" cellpadding="0" width="100%" style="width:100.0%;border-collapse:collapse;border:none;mso-border-alt:solid windowtext .5pt;
+
−
mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt">
+
−
<tbody><tr style="mso-yfti-irow:0;mso-yfti-firstrow:yes;height:67.3pt">
+
−
  <td width="16%" style="width:16.22%;border:solid windowtext 1.0pt;mso-border-alt:
+
−
  solid windowtext .5pt;background:#CFCFCF;padding:0cm 5.4pt 0cm 5.4pt;
+
−
  height:67.3pt">
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">PART NAME /<br> NO.</span><o:p></o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  </td>
+
−
  <td width="12%" style="width:12.56%;border:solid windowtext 1.0pt;border-left:
+
−
  none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+
−
  background:#CFCFCF;padding:0cm 5.4pt 0cm 5.4pt;height:67.3pt">
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>SOURCE<o:p></o:p></span></p>
+
−
  </td>
+
−
  <td width="30%" style="width:30.12%;border:solid windowtext 1.0pt;border-left:
+
−
  none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+
−
  background:#CFCFCF;padding:0cm 5.4pt 0cm 5.4pt;height:67.3pt">
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>KEY FEATURES<o:p></o:p></span></p>
+
−
  </td>
+
−
  <td width="41%" style="width:41.1%;border:solid windowtext 1.0pt;border-left:
+
−
  none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+
−
  background:#CFCFCF;padding:0cm 5.4pt 0cm 5.4pt;height:67.3pt">
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>WHY IS IT SILVER?<o:p></o:p></span></p>
+
−
  </td>
+
−
</tr>
+
−
<tr style="mso-yfti-irow:1;mso-yfti-lastrow:yes;height:11.35pt">
+
−
  <td width="16%" style="width:16.22%;border:solid windowtext 1.0pt;border-top:
+
−
  none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+
−
  padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt">
+
−
    <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
      <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
      <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Cytoplasmic-<span class="SpellE">Winsulin</span><o:p></o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417003">BBa_K2417003</a><o:p></o:p></span></p>
+
−
  </td>
+
−
  <td width="12%" style="width:12.56%;border-top:none;border-left:none;
+
−
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt">
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>N/A </span><span lang="EN-US" style="font-size:14.0pt;font-family:Helvetica;mso-fareast-font-family:Helvetica;
+
−
  mso-bidi-font-family:Helvetica">–</span><span lang="EN-US" style="font-size:
+
−
  14.0pt;font-family:Quicksand"> <span class="SpellE">Winsulin</span> is <span class="SpellE">self designed</span> from literature and mammalian insulins<o:p></o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  </td>
+
−
  <td width="30%" style="width:30.12%;border-top:none;border-left:none;
+
−
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt">
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Novel single chain insulin
+
−
  analogue (<span class="SpellE">Winsulin</span>) with a 12AA linker (QRGGGSGGGQRR)<o:p></o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N-terminal 6x His Tag<o:p></o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">4x GSS flexible linker<o:p></o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">TEV protease cleavage site<o:p></o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Extended ribosome binding site<o:p></o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  </td>
+
−
  <td width="41%" valign="top" style="width:41.1%;border-top:none;border-left:
+
−
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:11.35pt">
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoListParagraph" style="text-indent:-00.0pt;mso-list:l12 level1 lfo22"><!--[if !supportLists]--><span lang="EN-US" style="font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
+
−
  Symbol;mso-bidi-font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt &quot;Times New Roman&quot;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><!--[endif]--><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">We validated our
+
−
  parts by showing that they work in producing insulin. We performed an ELISA
+
−
  assay testing for properly folded insulin, and detected the presence of
+
−
  Cytoplasmic <span class="SpellE">Winsulin</span>.<o:p></o:p></span></p>
+
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand">The
+
−
  results for the assay can be found <a href="https://2017.igem.org/Team:Sydney_Australia/Results">here</a><o:p></o:p></span></p>
+
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:15.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  </td>
+
−
</tr>
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</tbody></table>
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</div>
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</div>
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</div>
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<div class="row" style="height:5vw;"></div>
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<div class = "row">
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<div class = "content" id="please">
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<center><h1>BRONZE</h1>
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<div class="box3"></div></center>
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−
<div class="row" style="height:2vw;"></div>
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<div align="center">
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−
<div align="center">
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+
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<table class="MsoTableGrid" border="1" cellspacing="0" cellpadding="0" width="100%" style="width:100.0%;border-collapse:collapse;border:none;mso-border-alt:solid windowtext .5pt;
+
−
mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt">
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−
<tbody><tr style="mso-yfti-irow:0;mso-yfti-firstrow:yes;height:4.75pt">
+
−
  <td width="16%" style="width:16.22%;border:solid windowtext 1.0pt;mso-border-alt:
+
−
  solid windowtext .5pt;background:#CD8B65;padding:0cm 5.4pt 0cm 5.4pt;
+
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  height:4.75pt">
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">PART NAME /<br> NO.<o:p></o:p></span></p>
+
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
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  </td>
+
−
  <td width="12%" style="width:12.48%;border:solid windowtext 1.0pt;border-left:
+
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  none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+
−
  background:#CD8B65;padding:0cm 5.4pt 0cm 5.4pt;height:4.75pt">
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>SOURCE<o:p></o:p></span></p>
+
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  </td>
+
−
  <td width="30%" style="width:30.2%;border:solid windowtext 1.0pt;border-left:
+
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  none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+
−
  background:#CD8B65;padding:0cm 5.4pt 0cm 5.4pt;height:4.75pt">
+
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  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>KEY FEATURES<o:p></o:p></span></p>
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  </td>
+
−
  <td width="41%" style="width:41.1%;border:solid windowtext 1.0pt;border-left:
+
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  none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+
−
  background:#CD8B65;padding:0cm 5.4pt 0cm 5.4pt;height:4.75pt">
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>WHY IS IT BRONZE?<o:p></o:p></span></p>
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  </td>
+
−
</tr>
+
−
<tr style="mso-yfti-irow:1;height:35.15pt">
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  <td width="16%" rowspan="2" style="width:16.22%;border:solid windowtext 1.0pt;
+
−
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+
−
  padding:0cm 5.4pt 0cm 5.4pt;height:35.15pt">
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span class="SpellE"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Ecotin-Winsulin</span></span><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p></o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><a href="http://parts.igem.org/Part:BBa_K2417002">BBa_K2417002</a><o:p></o:p></span></p>
+
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  </td>
+
−
  <td width="12%" style="width:12.48%;border-top:none;border-left:none;
+
−
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:35.15pt">
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><br><br>E. Coli (<span class="SpellE">Ecotin</span>
+
−
  Tag)<o:p></o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  </td>
+
−
  <td width="30%" rowspan="2" style="width:30.2%;border-top:none;border-left:
+
−
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:35.15pt">
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Novel single chain insulin
+
−
  analogue (<span class="SpellE">Winsulin</span>) with a 12AA linker (QRGGGSGGGQRR)<o:p></o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N-terminal <span class="SpellE">Ecotin</span>
+
−
  tag for targeting proteins to the periplasm<o:p></o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N-terminal 6x His Tag<o:p></o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">4x GSS flexible linker<o:p></o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">TEV protease cleavage site<o:p></o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">Extended ribosome binding site<o:p></o:p></span></p>
+
−
  </td>
+
−
  <td width="41%" rowspan="2" style="width:41.1%;border-top:none;border-left:
+
−
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:35.15pt">
+
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">We submitted this DNA part to
+
−
  the registry, for other teams to use in the future<o:p></o:p></span></p>
+
−
  </td>
+
−
</tr>
+
−
<tr style="mso-yfti-irow:2;mso-yfti-lastrow:yes;height:18.0pt">
+
−
  <td width="12%" style="width:12.48%;border-top:none;border-left:none;
+
−
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
−
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+
−
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:18.0pt">
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand">N/A </span><span lang="EN-US" style="font-size:14.0pt;font-family:Helvetica;mso-fareast-font-family:Helvetica;
+
−
  mso-bidi-font-family:Helvetica">–</span><span lang="EN-US" style="font-size:
+
−
  14.0pt;font-family:Quicksand"> <span class="SpellE">Winsulin</span> is <span class="SpellE">self designed</span> from literature and mammalian insulins<o:p></o:p></span></p>
+
−
  <p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-US" style="font-size:14.0pt;font-family:Quicksand"><o:p>&nbsp;</o:p></span></p>
+
−
  </td>
+
−
</tr>
+
−
</tbody></table>
+
−
 
+
−
</div>
+
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</div>
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</div>
+
 
</div>
 
</div>
  

Revision as of 10:20, 31 October 2017