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<img class="figure image" src="https://static.igem.org/mediawiki/2017/e/e6/T--Bielefeld-CeBiTec--YKE_medal_silver_icon.png"> | <img class="figure image" src="https://static.igem.org/mediawiki/2017/e/e6/T--Bielefeld-CeBiTec--YKE_medal_silver_icon.png"> | ||
<p class="figure subtitle"></p></div> | <p class="figure subtitle"></p></div> | ||
− | <h4> Validated Part | + | <h4> Validated Part</h4> |
<article> | <article> | ||
In our project we used and created various parts. We want to highlight part <a target="_blank" href="http://parts.igem.org/Part:BBa_K2201004">BBa_K2201004</a> which functioned just as expected and was well characterized and validated. | In our project we used and created various parts. We want to highlight part <a target="_blank" href="http://parts.igem.org/Part:BBa_K2201004">BBa_K2201004</a> which functioned just as expected and was well characterized and validated. | ||
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We created an <a target="_blank" href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Attributions">attribution</a> site where we listed and thanked all the people who supported us with advice and material throughout our project. If not mentioned specifically we have performed the work ourselves and the results are due to our own work. | We created an <a target="_blank" href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Attributions">attribution</a> site where we listed and thanked all the people who supported us with advice and material throughout our project. If not mentioned specifically we have performed the work ourselves and the results are due to our own work. | ||
</article> | </article> | ||
− | <h4> Characterization | + | <h4> Characterization</h4> |
<div class="article"> | <div class="article"> | ||
We characterized various <a target="_blank" href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Parts">parts</a> and successfully participated at the <a target="_blank" href="https://2017.igem.org/Team:Bielefeld-CeBiTec/InterLab"> Interlab Measurement Study </a>.</div> | We characterized various <a target="_blank" href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Parts">parts</a> and successfully participated at the <a target="_blank" href="https://2017.igem.org/Team:Bielefeld-CeBiTec/InterLab"> Interlab Measurement Study </a>.</div> |
Revision as of 14:13, 31 October 2017
![](https://static.igem.org/mediawiki/2017/7/74/T--Bielefeld-CeBiTec--title-img-centrifuge.jpg)
Achievements
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Establishment of two orthogonal methods for the detection of unnatural base pairs in a target sequence via Oxford Nanopore sequencing and an enzyme based detection method
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Development of a software suite for these orthogonal methods
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Integration and characterization of the nucleotide transporter PtNTT2 from P.tricornutum in E.coli for the uptake of unnatural nucleoside triphosphates
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Proof that certain Taq-polymerases can efficiently incorporate unnatural nucleotides
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Construction of a toolbox consisting of five aminoacyl-tRNA synthetases for incorporation of non-canonical amino acids
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Development of a photoswitchable lycopene pathway
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Design, chemical synthesis and proof of functionality of a novel, fully synthetic amino acid based on cyanonitrobenzothiazol and asparagine
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Modeling more than ten new aaRS sequences
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Library development with several hundred thousand sequences for selecting aminoacyl-tRNA synthetases
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Construction of positive and negative selection plasmids for the evolution of new synthetases for non-canonical amino acids
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Improvement of an aminoacyl-tRNA synthetase test-system by introducing a FRET-system and development of a ranking system
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Construction of an LED panel for irradiating 96-well microtiter plates, which can be used to manipulate non-canonical amino acids and much more
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Development of an Android App to control the LED panel with your smartphone via Bluetooth
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Writing a biosafety report titled “Auxotrophy to Xeno-DNA: A Comprehensive Exploration of Combinatorial Mechanisms for a High-Fidelity Biosafety System”
![](https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png)
Writing the ChImp Report on “Chances and Implications of an Expanded Genetic Code”
We Applied for the following Special Prizes
Best Integrated Human Practices
Best Education and Public Engagement
Best Measurement
Best Modeling
Best applied design
Best Basic Part
Best Composite Part
Best Part Collection
Best Software Tool
Best Hardware
![](https://static.igem.org/mediawiki/2017/2/28/T--Bielefeld-CeBiTec--YKE_medal_gold_icon.png)
Integrated Human Practices
Improve a previous part
We improved the validation system for aminoacyl-tRNA synthetases for ncAAs from Austin Texas 2014 (BBa_K1416004) and Aachen 2016 (BBa_K2020040) with a FRET system (BBa_K2201343) and used it in our project. You can find our part improvement site here.
Model your project
Demonstrate your work
![](https://static.igem.org/mediawiki/2017/e/e6/T--Bielefeld-CeBiTec--YKE_medal_silver_icon.png)
Validated Part
Collaboration
Human Practices
![](https://static.igem.org/mediawiki/2017/5/52/T--Bielefeld-CeBiTec--YKE_medal_bronze_icon.png)
Register and attend
Deliverables
Attribution
Characterization
We characterized various parts and successfully participated at the Interlab Measurement Study .