Revision as of 23:19, 29 June 2017

Engineering of N-Acyl Homoserine Lactone (AHL) genes in the quorum-sensing of Pseudomonas

Hundreds of millions of patients every year acquire nosocomial infections according to the World Health Organisation. Treatment is complicated by antibiotic resistance with a rapid increase of multi-drug resistance (MDR) bacteria, including strains of the gram-negative genera: Pseudomonas and Escherichia. Opportunistic Pseudomonas are responsible for 10% of all global nosocomial infections in immunocompromised individuals with 50-90% of patients of cystic fibrosis patients suffering from a Pseudomonas Aeruginosa infection. A major contributor to antibiotic resistance is bacterial aggregation to form biofilms. Bacteria within biofilms communicate through chemicals, such as N-Acyl homoserine lactones (AHL), quorum-sensing molecules essential to biofilm formation. We aim to develop strategies to inhibit biofilm formation by targeting AHL regulation genes: ppuR, ppuA, ppuI and RsaL in Pseudomonas putida. This project has a vast range of potential therapeutic applications such as the development of biocontainment devices and will provide fundamental information to understanding and developing tools in combating antibiotic resistance.

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@@ Line 11: / Line 11: @@
 <div class="column full_size" >
 <h1> Engineering of N-Acyl Homoserine Lactone (AHL) genes in the quorum-sensing of Pseudomonas</h1>
-<p>Hundreds of millions of patients every year acquire an infection from their healthcare setting, according to the World Health Organisation. Treating these nosocomial infections is complicated by antibiotic resistance; there has been a rapid increase of multidrug resistance (MDR) bacteria, including strains of the gram-negative genera, Pseudomonas and Escherichia. Opportunistic Pseudomonas are responsible for 10% of all global hospital acquired infections which cause disease in immuno-deficient individuals. In cystic fibrosis, Pseudomonas aeruginosa is estimated to colonise the lungs of 50-90% of patients.
+<p>Hundreds of millions of patients every year acquire nosocomial infections according to the World Health Organisation. Treatment is complicated by antibiotic resistance with a rapid increase of multi-drug resistance (MDR) bacteria, including strains of the gram-negative genera: Pseudomonas and Escherichia. Opportunistic Pseudomonas are responsible for 10% of all global nosocomial infections in immunocompromised individuals with 50-90% of patients of cystic fibrosis patients suffering from a Pseudomonas Aeruginosa infection.
-A major contributor to bacterial antibiotic resistance is the aggregation of bacteria to form biofilms. Bacteria within biofilms communicate with each other through the release of chemicals, such as N-Acyl homoserine lactones (AHL), these quorum-sensing molecules are essential to biofilm formation. This project will aim to develop strategies to inhibit biofilm formation by targeting the specific genes responsible for AHL regulation; ppuR, ppuA, ppuI and RsaL in Pseudomonas putida. This project has a vast range of potential therapeutic applications such as development of biocontainment devices against MDR bacteria. This project will provide a fundamental contribution to understanding and combating antibiotic resistance with applications for treating nosocomical infections.
+A major contributor to  antibiotic resistance is  bacterial aggregation to form biofilms. Bacteria within biofilms communicate through chemicals, such as N-Acyl homoserine lactones (AHL), quorum-sensing molecules essential to biofilm formation. We  aim to develop strategies to inhibit biofilm formation by targeting AHL regulation genes: ppuR, ppuA, ppuI and RsaL in Pseudomonas putida. This project has a vast range of potential therapeutic applications such as the development of biocontainment devices and will provide fundamental information to understanding and developing tools in combating antibiotic resistance.
   </p>
 </div>

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