Difference between revisions of "Team:BostonU HW/Digestion"

 
(15 intermediate revisions by 3 users not shown)
Line 18: Line 18:
 
}
 
}
 
#page_background{
 
#page_background{
−
background-image: url("https://static.igem.org/mediawiki/2017/0/04/MARS_General_Background.png");
+
        background-image: url("https://static.igem.org/mediawiki/2017/9/94/LARGE_background_MARS.png");
−
}
+
        background-size:100% ;
 +
    }
 
#Header_Pic{
 
#Header_Pic{
 
height: 60%;
 
height: 60%;
Line 65: Line 66:
 
<div class="wrapper" id="page_background">
 
<div class="wrapper" id="page_background">
 
<div class="header" id="Header_Pic">
 
<div class="header" id="Header_Pic">
−
<img src="https://static.igem.org/mediawiki/2017/0/02/MARSbackground.png" id="BACKGROUND">
+
<img src="https://static.igem.org/mediawiki/2017/9/94/LARGE_background_MARS.png" id="BACKGROUND">
−
<img src="https://static.igem.org/mediawiki/2017/2/22/MARSLogo2.png" id="MARS">
+
<div class="container" margin-top:"2%;">
−
<img src="https://static.igem.org/mediawiki/2017/7/7a/MARS_Digestion.png" id="TITLE">
+
<div class="col-md-3">
 +
 
 +
<img src="https://static.igem.org/mediawiki/2017/2/22/MARSLogo2.png" width="100%" style="margin-top:-37%;">
 +
</div>
 +
<div class="col-md-9" style="color:#eef1f5; font-size:100px; font-family:Arial,Gadget,sans-serif; margin-top:1%;">
 +
DNA Digestion
 +
</div>
 +
</div>
 
</div>
 
</div>
−
<div class="main main-raised" style="margin-bottom:5%;">
+
<div class="main main-raised" style="margin-top:2%;">
 
<div class="container">
 
<div class="container">
−
<div class="col-md-9">
+
<div class="col-md-12">
 
<div class="text_section">
 
<div class="text_section">
 
<h1>Summary</h1>
 
<h1>Summary</h1>
Line 78: Line 86:
 
</div>
 
</div>
 
</div>
 
</div>
−
</div>
 
−
<div class="col-md-3" style="text-align:center; margin:auto; vertical-align:middle;" >
 
−
<a href="MARStest.zip"download>
 
−
<button class="btn btn-primary btn-lg btn-danger">Download Files Here!<i class="material-icons">get_app</i></button>
 
−
</a>
 
 
</div>
 
</div>
 
</div>
 
</div>
Line 88: Line 91:
 
<div class="col-md-9">
 
<div class="col-md-9">
 
<div class="text">
 
<div class="text">
−
This microfluidic chip design performs DNA digestion. The desired DNA segment, restriction enzymes, water and buffer solution are inputted and mixed. The resulting solution is then sealed in an incubation chamber on top of which a heating element is placed. After the designated incubation time has passed, the liquid can then be transferred from the chip using a pipett
+
This microfluidic chip design performs DNA digestion. The desired DNA segment, restriction enzymes, water and buffer solution are inputted and mixed. The resulting solution is then sealed in an incubation chamber on top of which a heating element is placed. After the designated incubation time has passed, the liquid can then be transferred from the chip using a pipette.
 +
                                <br><br>
 +
This chip has been milled and tested, but not deemed fully fluid functional as of this time. For a more complete understanding of the chip, click the download button in order to access its CNC millable SVG files, JSON file, full device documentation and PNG files of its flow and control layers.
 +
 
 
</div>
 
</div>
 
</div>
 
</div>
 
<div class="col-md-3">
 
<div class="col-md-3">
 +
<div style="text-align:center; margin:auto; vertical-align:middle;" >
 +
  <a href="https://2017.igem.org/Team:BostonU_HW/Demonstrate#Chips">
 +
    <button class="btn btn-primary btn-lg btn-danger" style="margin-top:0px;">MARS Repository<i class="material-icons">replay</i></button>
 +
  </a>
 +
</div>
 +
<div style="text-align:center; margin:auto; vertical-align:middle;" >
 +
<a href="https://static.igem.org/mediawiki/2017/7/70/MARSDigestion.zip"download>
 +
<button class="btn btn-primary btn-lg btn-danger">Download Files Here!<i class="material-icons">get_app</i></button>
 +
</a>
 +
</div>
 
<div class="profile-tabs">
 
<div class="profile-tabs">
 
<div class="nav-align-center">
 
<div class="nav-align-center">
Line 147: Line 163:
 
</div>
 
</div>
 
</div>
 
</div>
 +
 +
<!-- THIS IS FOOTER -->
 +
<div class="wrapper" style="background:#1c1f1f; margin-top:0px;margin-right:0px !important; margin-left:0px !important;" id="Footer">
 +
<div class="container" style="text-align:center !important">
 +
 +
<div class="col-md-2" style="color:white; margin-bottom:30px; margin-top:5px;">
 +
<h3>CONTACT US</h3>
 +
<div style="text-align:center;">
 +
<a href="mailto:igembuhw@gmail.com">
 +
<img src="https://static.igem.org/mediawiki/2017/7/74/MARS_WHITEEmail.png" style="height:60px; margin-top:20px;">
 +
</a>
 +
<a href="https://www.instagram.com/buigemhardware/?hl=en">
 +
<img src="https://static.igem.org/mediawiki/2017/9/93/MARS_Final_insta.png" style="height:60px; margin-top:20px;">
 +
</a>
 +
<a href="https://twitter.com/igemhwbu">
 +
<img src="https://static.igem.org/mediawiki/2017/b/b6/MARS_Twitter_White.png" style="height:60px; margin-top:20px;">
 +
</a>
 +
</div>
 +
</div>
 +
<div class="col-md-10" style="margin-bottom:30px;">
 +
<img src="https://static.igem.org/mediawiki/2017/0/0e/MARS_SponsorsFinal.png" style="width:100%; margin-top:30px;" usemap="#image-map">
 +
</div>
 +
</div>
 +
 +
</div>
 +
 +
 
</div>
 
</div>
 
</body>
 
</body>

Latest revision as of 18:12, 31 October 2017

BostonU_HW

Digestion
DNA Digestion

Summary

DNA digestion, or DNA fragmentation, is a basic protocol in synthetic biology. This is typically performed prior to analysis of the DNA sequence, or in order to perform further protocols. Restriction enzymes are mixed and then incubated with DNA in a buffer solution. This yields DNA fragments cleaved at specific sites according to the enzymes used.
This microfluidic chip design performs DNA digestion. The desired DNA segment, restriction enzymes, water and buffer solution are inputted and mixed. The resulting solution is then sealed in an incubation chamber on top of which a heating element is placed. After the designated incubation time has passed, the liquid can then be transferred from the chip using a pipette.

This chip has been milled and tested, but not deemed fully fluid functional as of this time. For a more complete understanding of the chip, click the download button in order to access its CNC millable SVG files, JSON file, full device documentation and PNG files of its flow and control layers.
Picture
Picture
Picture
Picture