Difference between revisions of "Team:BostonU HW/PCR"

 
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background-image: url("https://static.igem.org/mediawiki/2017/0/04/MARS_General_Background.png");
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background-image: url("https://static.igem.org/mediawiki/2017/9/94/LARGE_background_MARS.png");
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<div class="wrapper" id="page_background">
 
<div class="wrapper" id="page_background">
 
<div class="header" id="Header_Pic">
 
<div class="header" id="Header_Pic">
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<img src="https://static.igem.org/mediawiki/2017/0/02/MARSbackground.png" id="BACKGROUND">
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<img src="https://static.igem.org/mediawiki/2017/9/94/LARGE_background_MARS.png" id="BACKGROUND">
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<img src="https://static.igem.org/mediawiki/2017/2/22/MARSLogo2.png" id="MARS">
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<div class="container" margin-top:"2%;">
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<img src="https://static.igem.org/mediawiki/2017/e/ee/MARS_CellSorting.png" id="TITLE">
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<div class="col-md-3">
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<img src="https://static.igem.org/mediawiki/2017/2/22/MARSLogo2.png" width="100%" style="margin-top:-37%;">
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</div>
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<div class="col-md-9" style="color:#eef1f5; font-size:100px; font-family:Arial,Gadget,sans-serif; margin-top:1%;">
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PCR
 
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<div class="col-md-12">
 
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<div class="text_section">
 
<h1>Summary</h1>
 
<h1>Summary</h1>
 
<div class="text" style="margin-bottom:3%;">
 
<div class="text" style="margin-bottom:3%;">
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The ability to sort cells by type or physical properties is a valuable tool in many synthetic biology labs. Prior to analysis or in order to perform specialised protocols, creating homogenous cell suspensions from a mixture is necessary. In addition to sorting cells, the removal of cell fragments, activated magnetic particles or unwanted debris through sorting also makes up a key part of purification protocols.
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PCR, or polymerase chain reaction, is a commonly used protocol in synthetic biology in order to replicate target DNA fragments. Through the use of specific primers, polymerases, and temperature cycling, exponential replicas of the target DNA fragment can be made. These fragments can then be ligated into plasmids and transformed into bacteria.
 
</div>
 
</div>
 
</div>
 
</div>
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</div>
 
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<div class="col-md-3" style="text-align:center; margin:auto; vertical-align:middle;" >
 
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<a href="MARStest.zip"download>
 
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<button class="btn btn-primary btn-lg btn-danger">Download Files Here!<i class="material-icons">get_app</i></button>
 
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</a>
 
 
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This microfluidic chip design carries out cell sorting as a cell suspension is passed through it. Cells are sorted based on size and pushed to the periphery of the channel. These cells are then carried away from the main solution through the two periphery outputs, and the cell-free solution can be collected from the central output.
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This microfluidic chip is designed to perform PCR. A solution containing the required substrates for PCR is flowed into the chip and through a mixer, on top of chip a heating element will be placed. The valves at both ends of the mixer are closed, isolating the mixture, and then the heating element performs temperature cycling. The liquid is then pushed out into an output receptacle for future use in the lab.
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                                <br><br>
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This chip has been milled and tested, but not deemed fully fluid functional as of this time. For a more complete understanding of the chip, click the download button in order to access its CNC millable SVG files, JSON file, full device documentation and PNG files of its flow and control layers.
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</div>
 
</div>
 
</div>
 
</div>
 
<div class="col-md-3">
 
<div class="col-md-3">
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<div style="text-align:center; margin:auto; vertical-align:middle;" >
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  <a href="https://2017.igem.org/Team:BostonU_HW/Demonstrate#Chips">
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    <button class="btn btn-primary btn-lg btn-danger" style="margin-top:0px;">MARS Repository<i class="material-icons">replay</i></button>
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  </a>
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</div>
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<div style="text-align:center; margin:auto; vertical-align:middle;" >
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<a href="https://static.igem.org/mediawiki/2017/9/92/MARSPCR.zip"download>
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<button class="btn btn-primary btn-lg btn-danger">Download Files Here!<i class="material-icons">get_app</i></button>
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</a>
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<div class="profile-tabs">
 
<div class="profile-tabs">
 
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<div class="container">
 
<div class="container">
 
<div class="tab-content gallery text-center">
 
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<div class="tab-pane active" id="Design">
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<div class="tab-pane active" id="Design">
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<div class="col-md-12 text-center">
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<div class="col-md-6 text-center">
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<img class="pics" src="https://static.igem.org/mediawiki/2017/6/69/MARS_CellSorting_F.png" alt="Picture" style="margin-top:20px; padding-top:18px;">
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<img class="pics" src="https://static.igem.org/mediawiki/2017/8/87/MARS_PCR_F.png" alt="Picture" style="margin-top:20px; padding-top:18px;">
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<div class="row text-center">
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<div class="row text-center" style="margin-bottom:5%;">
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<button class="btn btn-info btn-round"><span style="font-size:17px;">Flow Layer</span></button>
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<button class="btn btn-info btn-round" ><span style="font-size:17px;">Flow Layer</span></button>
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<div class="tab-pane" id="Mill" style="margin-bottom:5%;">
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<img class="pics" src="https://static.igem.org/mediawiki/2017/e/e3/MARS_PCR_C.png" alt="Picture" style="margin-top:20px; padding-top:18px;">
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<img class="pics" src="https://static.igem.org/mediawiki/2017/thumb/7/7f/MARS_CellSorting_MF.png/800px-MARS_CellSorting_MF.png.jpeg" alt="Picture" style="margin-top:20px; padding-top:18px; width:45% !important;">
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<button class="btn btn-danger btn-round"><span style="font-size:17px;">Control Layer</span></button>
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</div>
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<button class="btn btn-info btn-round"><span style="font-size:17px;">Flow Layer</span></button>
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<div class="tab-pane" id="Mill">
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<div class="col-md-6 text-center">
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<img class="pics" src="https://static.igem.org/mediawiki/2017/a/a6/MARS_PCR_MF.png" alt="Picture" style="margin-top:20px; padding-top:18px;">
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<div class="row">
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<button class="btn btn-info btn-round" style="margin-bottom:5%;"><span style="font-size:17px;">Flow Layer</span></button>
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</div>
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</div>
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<div class="col-md-6 text-center">
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<img class="pics" src="https://static.igem.org/mediawiki/2017/4/47/MARS_PCR_MC.png" alt="Picture" style="margin-top:20px; padding-top:18px;">
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<div class="row text-center">
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<button class="btn btn-danger btn-round" style="margin-bottom:5%;"><span style="font-size:17px;">Control Layer</span></button>
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</div>
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</div>
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</div>
 
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<!-- THIS IS FOOTER -->
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<div class="wrapper" style="background:#1c1f1f; margin-top:0px;margin-right:0px !important; margin-left:0px !important;" id="Footer">
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<div class="container" style="text-align:center !important">
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<div class="col-md-2" style="color:white; margin-bottom:30px; margin-top:5px;">
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<h3>CONTACT US</h3>
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<div style="text-align:center;">
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<a href="mailto:igembuhw@gmail.com">
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<img src="https://static.igem.org/mediawiki/2017/7/74/MARS_WHITEEmail.png" style="height:60px; margin-top:20px;">
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</a>
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<a href="https://www.instagram.com/buigemhardware/?hl=en">
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<img src="https://static.igem.org/mediawiki/2017/9/93/MARS_Final_insta.png" style="height:60px; margin-top:20px;">
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</a>
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<a href="https://twitter.com/igemhwbu">
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<img src="https://static.igem.org/mediawiki/2017/b/b6/MARS_Twitter_White.png" style="height:60px; margin-top:20px;">
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</a>
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</div>
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</div>
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<div class="col-md-10" style="margin-bottom:30px;">
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<img src="https://static.igem.org/mediawiki/2017/0/0e/MARS_SponsorsFinal.png" style="width:100%; margin-top:30px;" usemap="#image-map">
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</div>
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</div>
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</div>
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</body>
 
</body>

Latest revision as of 18:14, 31 October 2017

BostonU_HW

PCR
PCR

Summary

PCR, or polymerase chain reaction, is a commonly used protocol in synthetic biology in order to replicate target DNA fragments. Through the use of specific primers, polymerases, and temperature cycling, exponential replicas of the target DNA fragment can be made. These fragments can then be ligated into plasmids and transformed into bacteria.
This microfluidic chip is designed to perform PCR. A solution containing the required substrates for PCR is flowed into the chip and through a mixer, on top of chip a heating element will be placed. The valves at both ends of the mixer are closed, isolating the mixture, and then the heating element performs temperature cycling. The liquid is then pushed out into an output receptacle for future use in the lab.

This chip has been milled and tested, but not deemed fully fluid functional as of this time. For a more complete understanding of the chip, click the download button in order to access its CNC millable SVG files, JSON file, full device documentation and PNG files of its flow and control layers.
Picture
Picture
Picture
Picture