Difference between revisions of "Team:BostonU HW/Contribution"

 
(11 intermediate revisions by 3 users not shown)
Line 18: Line 18:
 
}
 
}
 
#page_background{
 
#page_background{
−
background-image: url("https://static.igem.org/mediawiki/2017/0/04/MARS_General_Background.png");
+
background-image: url("https://static.igem.org/mediawiki/2017/9/94/LARGE_background_MARS.png");
 +
background-size:100%;
 
}
 
}
 
#BACKGROUND{
 
#BACKGROUND{
Line 54: Line 55:
 
<div class="wrapper" id="page_background">
 
<div class="wrapper" id="page_background">
 
<div class="header" id="Header_Pic">
 
<div class="header" id="Header_Pic">
−
<img src="https://static.igem.org/mediawiki/2017/0/02/MARSbackground.png" id="BACKGROUND">
+
<img src="https://static.igem.org/mediawiki/2017/9/94/LARGE_background_MARS.png" id="BACKGROUND">
−
<img src="https://static.igem.org/mediawiki/2017/2/22/MARSLogo2.png" id="MARS">
+
<div class="container" margin-top:"2%;">
−
<img src="https://static.igem.org/mediawiki/2017/6/69/MARS_Attributions.png" id="TITLE">
+
<div class="col-md-3">
 +
 
 +
<img src="https://static.igem.org/mediawiki/2017/2/22/MARSLogo2.png" width="100%" style="margin-top:-37%;">
 +
</div>
 +
<div class="col-md-9" style="color:#eef1f5; font-size:100px; font-family:Arial,Gadget,sans-serif; margin-top:1%;">
 +
Contribution
 +
</div>
 +
</div>
 
</div>
 
</div>
−
 
+
<div class="main main-raised" style="margin-top:2%;" id="uF_101">
−
<div class="main main-raised" style="margin-top:5%" id="uF_101">
+
 
<div class="container">
 
<div class="container">
−
<h2>Microfluidics 101</h2>
+
<h1 class="title text-center">Our Contribution</h1>
−
<h3>Introduction to Microfluidics | Dinithi</h3>
+
 
<div class="text">
 
<div class="text">
−
In order to effectively use the MARS system, users need to have a fundamental understanding of microfluidics. With this in mind, we created an introduction to microfluidics that teaches users the basics of microfluidics. This educational component of project MARS consists of an introduction to microfluidic chips as well as a guide to the various primitives used in our microfluidic designs.
+
Through Project MARS, the BostonU HW Team has provided three key contributions towards increasing ease of accessibility and use of microfluidics in the synthetic biology community.
−
</div>
+
 
−
</div>
+
  
−
<div class="container">
 
−
<h3>Video Tutorials | Sarah and Dinithi</h3>
 
−
<div class="text" style="margin-bottom:3%;">
 
−
In order to make the microfluidic chips we designed accessible to synthetic biologists we developed four fully-narrated tutorial videos. These videos go step by step through the process of manufacturing, assembling, and testing a microfluidic device. The four videos created teach uses how to mill a microfluidic chip, how to make PDMS, how to assemble a microfluidic chip, and how to clean a microfluidic chip. Each of these videos is accompanied by a detailed written protocol as well.
 
 
</div>
 
</div>
 
</div>
 
</div>
  
−
</div>
 
  
−
<div class="main main-raised" style="margin-top:5%" id ="Chip_Repo">
+
<div class="container"><h2>MARS Repository</h2>
 +
<div class="text">
 +
The MARS repository benefits the greater synthetic biology community through hosting the designs of nine chips that are designed to perform essential synbio procedures.
 +
                                        <br><br>
 +
                                        These chips are:<br>
 +
                                      </div>
 +
<ul>
 +
<li>Fabricated using a standardized rapid prototyping system</li>
 +
<li>Separated into three categories (isolation, modification and quantification) which come together to replicate complex synthetic biology protocols </li>
 +
<li>Provided to the user with full documentation and usage protocols</li>
 +
</ul> <br>
 +
                              <div class="text">
 +
This provides a highly accessible and relevant platform for synthetic biologists to access, fabricate, test and integrate microfluidics into their lab workflow. <br><br>
 +
While these microfluidic devices have only been tested with colored water and oil, they provide a framework that can be built upon by future iGEM teams to allow for biological testing and optimization.
 +
</div></div>
 +
 
 +
 
 
<div class="container">
 
<div class="container">
−
<h2>Mars Repository</h2>
+
<h2>Fluid Functionality</h2>
 
<div class="text">
 
<div class="text">
−
In order to make microfluidics a practical field for synthetic biologists, we created the MARS microfluidic chip repository. This repository hosts nine microfluidic chips that each perform a fundamental synthetic biological protocol. These nine protocols were determined after reaching out to synthetic biologists in the Boston University and iGEM communities. Each chip comes with all the required design files and documentation so that a synthetic biologist could download, manufacture, and test any chip in the repository. The nine chips in the MARS repository are as follows:
+
Our fluid functionality checklist allows synthetic biologists to grade microfluidic devices fabricated from the MARS archive or designed using our software workflow. Through creating and introducing a standardized method of analyzing device functionality, synthetic biologists interested in utilizing microfluidics can quantitatively and qualitatively rate their chips prior to utilizing them in laboratory procedures.
−
</div>
+
</div></div>
−
</div>
+
  
−
<div class="container">
+
<div class="container" ><h2>Microfluidics 101</h2>
−
<h3>Cellular Lysis | Dylan</h3>
+
<div class="text" style ="margin-bottom:5%">
−
</div>
+
In order to overcome the knowledge barrier that exists between synthetic biology and microfluidics, MARS hosts a variety of educational materials aimed at synbio researchers interested in using our workflow. <br><br>
−
<div class="container">
+
Microfluidics 101 focuses on:
−
<h3>DNA Digestion | Dinithi</h3>
+
                     
−
</div>
+
<ul>
−
<div class="container">
+
<li>Increasing ease of access to basic educational materials regarding microfluidics</li>
−
<h3>Cell Sorting | Dinithi</h3>
+
<li> Providing clear and relevant material regarding our continuous flow microfluidics devices </li>
−
</div>
+
<li>Including detailed video tutorials and written protocols outlining fabrication using Makerfluidics</li>
−
<div class="container">
+
</ul>  
−
<h3>Ligation | Sarah</h3>
+
                              <div class="text">
−
</div>
+
<br>All three components of MARS come together to help make microfluidics a more accessible and practical tool for synthetic biology labs. <br> <br><br></div>
−
<div class="container">
+
−
<h3>Transformation | Sarah</h3>
+
−
</div>
+
−
<div class="container">
+
−
<h3>PCR | Sarah</h3>
+
−
</div>
+
−
<div class="container">
+
−
<h3>Antibiotic Resistance | Dylan</h3>
+
−
</div>
+
−
<div class="container">
+
−
<h3>Fluorescence Testing | Dinithi</h3>
+
−
</div>
+
−
<div class="container">
+
−
<h3>Cell Culturing | Dylan</h3>
+
−
</div>
+
  
−
<div class="container" >
+
<h1 class="title text-center">Our End Product</h1>
−
<div class="text" style="margin-bottom:3%; margin-top:3%;">
+
<div class="text">
−
For more information regarding each chip, please see our <a href="https://2017.igem.org/Team:BostonU_HW/Archive">MARS repository page</a>
+
After 560 hours in the lab per team member working on: <br>
−
</div>
+
                                          </div>
−
</div>
+
<ul>
 +
                                        <li> Over 100 design iterations </li>
 +
                                        <li> 85 milled and tested chips  </li>
 +
                                        <li> 340 fluid tests  </li>
 +
                                        <li> 80 hours editing video and written content  </li>
 +
                                        </ul><br>
 +
                                <div class="text">
 +
                                We are proud to present our project MARS in its entirety. Please feel free to navigate our Wiki to learn more about each branch of the project and how it applies to the synthetic biology community at large. <br><br>
 +
                                </div>
  
 
</div>
 
</div>
  
−
<div class="main main-raised" style="margin-top:5%; margin-bottom:3%;" id="FF">
 
−
<div class="container">
 
−
<h2>Fluid Functionality</h2>
 
−
<div class="text">
 
−
In order to verify whether or not a microfluidic device is functional, a grading system was developed: the Fluid Functionality Checklist. This fluid functionality system was broken up into two portions: a qualitative checklist and a quantitative analysis.
 
−
</div>
 
−
</div>
 
  
−
<div class="container">
 
−
<h3>Qualitative Checklist | Dylan, Sarah, and Dinithi</h3>
 
−
<div class="text">
 
−
The qualitative portion of the fluid functionality checklist consists of various failure modes. These are visual cues that something has gone wrong while running a chip, such as liquid leaking out of a channel or primitive. If a chip passes each of these qualitative checks, it is deemed “fluid functional.” If a chip does not pass each of these qualitative checks, the user moves to the quantitative analysis to determine why the chip failed.
 
−
</div>
 
−
</div>
 
  
−
<div class="container">
 
−
<h3>Quantitative Analysis | Dylan and Sarah</h3>
 
−
<div class="text" style="margin-bottom:3%;">
 
−
The quantitative portion of the fluid functionality checklist consists of various quantitative analyses. Two forms of analysis are included in this portion of our evaluation system: physics-based primitive analysis and image processing-based analysis. Physics based analysis helps to determine if a qualitative failure occurred because the incorrect primitive dimensions and/or flow rate were used. Image processing analysis is used to evaluate the functionality of primitives such as mixers. Using these analyses, a user can both determine why their chip failed and evaluate the functionality of key primitives.
 
−
</div>
 
−
</div>
 
−
 
−
</div>
 
 
   </div>
 
   </div>
  
 
</div>
 
</div>
 +
 +
<!-- THIS IS FOOTER -->
 +
<div class="wrapper" style="background:#1c1f1f; margin-top:0px;margin-right:0px !important; margin-left:0px !important;" id="Footer">
 +
  <div class="container" style="text-align:center !important">
 +
 +
    <div class="col-md-2" style="color:white; margin-bottom:30px; margin-top:5px;">
 +
      <h3>CONTACT US</h3>
 +
    <div style="text-align:center;">
 +
      <a href="mailto:igembuhw@gmail.com">
 +
      <img src="https://static.igem.org/mediawiki/2017/7/74/MARS_WHITEEmail.png" style="height:60px; margin-top:20px;">
 +
      </a>
 +
        <a href="https://www.instagram.com/buigemhardware/?hl=en">
 +
        <img src="https://static.igem.org/mediawiki/2017/9/93/MARS_Final_insta.png" style="height:60px; margin-top:20px;">
 +
      </a>
 +
          <a href="https://twitter.com/igemhwbu">
 +
          <img src="https://static.igem.org/mediawiki/2017/b/b6/MARS_Twitter_White.png" style="height:60px; margin-top:20px;">
 +
          </a>
 +
      </div>
 +
      </div>
 +
      <div class="col-md-10" style="margin-bottom:30px;">
 +
        <img src="https://static.igem.org/mediawiki/2017/0/0e/MARS_SponsorsFinal.png" style="width:100%; margin-top:30px;" usemap="#image-map">
 +
    </div>
 +
</div>
 +
 +
</div>
 +
 
</body>
 
</body>
  

Latest revision as of 02:08, 1 November 2017

BostonU_HW

Attributions
Contribution

Our Contribution

Through Project MARS, the BostonU HW Team has provided three key contributions towards increasing ease of accessibility and use of microfluidics in the synthetic biology community.

MARS Repository

The MARS repository benefits the greater synthetic biology community through hosting the designs of nine chips that are designed to perform essential synbio procedures.

These chips are:
  • Fabricated using a standardized rapid prototyping system
  • Separated into three categories (isolation, modification and quantification) which come together to replicate complex synthetic biology protocols
  • Provided to the user with full documentation and usage protocols

This provides a highly accessible and relevant platform for synthetic biologists to access, fabricate, test and integrate microfluidics into their lab workflow.

While these microfluidic devices have only been tested with colored water and oil, they provide a framework that can be built upon by future iGEM teams to allow for biological testing and optimization.

Fluid Functionality

Our fluid functionality checklist allows synthetic biologists to grade microfluidic devices fabricated from the MARS archive or designed using our software workflow. Through creating and introducing a standardized method of analyzing device functionality, synthetic biologists interested in utilizing microfluidics can quantitatively and qualitatively rate their chips prior to utilizing them in laboratory procedures.

Microfluidics 101

In order to overcome the knowledge barrier that exists between synthetic biology and microfluidics, MARS hosts a variety of educational materials aimed at synbio researchers interested in using our workflow.

Microfluidics 101 focuses on:
  • Increasing ease of access to basic educational materials regarding microfluidics
  • Providing clear and relevant material regarding our continuous flow microfluidics devices
  • Including detailed video tutorials and written protocols outlining fabrication using Makerfluidics

All three components of MARS come together to help make microfluidics a more accessible and practical tool for synthetic biology labs.


Our End Product

After 560 hours in the lab per team member working on:
  • Over 100 design iterations
  • 85 milled and tested chips
  • 340 fluid tests
  • 80 hours editing video and written content

We are proud to present our project MARS in its entirety. Please feel free to navigate our Wiki to learn more about each branch of the project and how it applies to the synthetic biology community at large.