Difference between revisions of "Team:BostonU HW/Notebook"

(Prototype team page)
 
 
(14 intermediate revisions by 3 users not shown)
Line 1: Line 1:
−
{{BostonU_HW}}
+
{{Global_Menubar}}
−
<html>
+
{{Team:BostonU_Hardware/CSS}}
 +
<html lang="en">
 +
<head>
 +
<meta charset="utf-8" />
 +
<link rel="apple-touch-icon" sizes="76x76" href="assets/img/apple-icon.png">
 +
<link rel="icon" type="image/png" href="assets/img/favicon.png">
 +
<meta http-equiv="X-UA-Compatible" content="IE=edge,chrome=1" />
  
 +
<title>Attributions</title>
  
−
<div class="column full_size">
+
<meta content='width=device-width, initial-scale=1.0, maximum-scale=1.0, user-scalable=0' name='viewport' />
  
−
<h1>Notebook</h1>
+
<!-- EXTRA STYLING -->
−
<p> Document the dates you worked on your project. This should be a detailed account of the work done each day for your project.</p>
+
<style>
 +
#Title{
 +
color: red;
 +
}
 +
#page_background{
 +
background-image: url("https://static.igem.org/mediawiki/2017/9/94/LARGE_background_MARS.png");
 +
background-size:100%;
 +
}
 +
#BACKGROUND{
 +
width: 100%;
 +
position: absolute;
 +
}
 +
#MARS{
 +
width: 15%;
 +
position: absolute;
 +
margin-top: 8%;
 +
margin-left: 5%;
 +
}
 +
#TITLE{
 +
position: absolute;
 +
width: 40%;
 +
margin-left: 22%;
 +
margin-top: 11%;
 +
}
 +
.main{
 +
margin-top: 2%;
 +
}
 +
#Header_Pic{
 +
height: 60%;
 +
}
 +
#Paragraph{
 +
text-align: center;
 +
}
 +
</style>
  
 +
</head>
 +
 +
<!-- *************THIS IS WHERE PAGE STARTS************* -->
 +
<body>
 +
<div class="landing-page">
 +
<div class="wrapper" id="page_background">
 +
<div class="header" id="Header_Pic">
 +
<img src="https://static.igem.org/mediawiki/2017/9/94/LARGE_background_MARS.png" id="BACKGROUND">
 +
<div class="container" margin-top:"2%;">
 +
<div class="col-md-3">
 +
 +
<img src="https://static.igem.org/mediawiki/2017/2/22/MARSLogo2.png" width="100%" style="margin-top:-37%;">
 +
</div>
 +
<div class="col-md-9" style="color:#eef1f5; font-size:100px; font-family:Arial,Gadget,sans-serif; margin-top:1%;">
 +
Notebook
 +
</div>
 
</div>
 
</div>
−
<div class="clear"></div>
+
</div>
 +
<div class="main main-raised" style="margin-top:2%;">
 +
<div class="container">
 +
<div class="text" style="margin-bottom:3%;">
 +
<h1>May</h1>
 +
<ul>
 +
<li>The BostonU Wetlab and Hardware iGEM teams participated in joint Wetlab safety and protocol training</li>
 +
<li>The team performed an initial literature review in order to learn more about the types of microfluidic devices being design</li>
 +
<li>
 +
The team was given a software overview/explanation of the three CIDAR lab microfluidic design tools: 3Duf, Fluigi, and Neptune
 +
</li>
 +
<li>The team was given an overview/explanation of the CIDAR lab microfluidic design manufacturing process: Makerfluidics</li>
 +
</ul>
 +
</div>
 +
</div>
 +
</div>
  
 +
<div class="main main-raised" style="margin-top:5%" id="uF_101">
 +
<div class="container">
 +
<div class="text" style="margin-bottom:3%;">
 +
<h1>June</h1>
 +
<ul>
 +
<li>From the  literature review, each member chose one chip of interest to replicate and test</li>
 +
<ol>
 +
<li>LAMP Chip<sup>[1]</sup>
 +
<ul>
 +
<li>3 iterations designed in Fluigi</li>
 +
<li>5 iterations designed in 3Duf and tested</li>
 +
</ul>
 +
</li>
 +
<li>Multiplex Drug Testing Chip<sup>[2]</sup></li>
 +
<ul>
 +
<li>2 iterations designed in 3Duf and tested</li>
 +
<li>1 iteration designed in Fluigi</li>
 +
</ul>
 +
<li>Magnetic Mixer Chip<sup>[3]</sup></li>
 +
<ul>
 +
<li>5 Iterations milled and tested</li>
 +
<li>Magnetic particles introduced into chip</li>
 +
<li>Experimented with different protocols</li>
 +
</ul>
 +
</ol>
 +
<li>BostonU Wetlab iGEM Team Collaboration began</li>
 +
<ul>
 +
<li>At the initial meeting BostonU Wetlab provided BostonU Hardware with a protocol they thought could be performed in a microfluidic device</li>
 +
<li>6 potential chips designed for Wetlab collaboration</li>
 +
</ul>
 +
<li>Submitted Microfluidics iGEM Poll to the iGEM collaborations page</li>
 +
<li>Volume Dispensing Chips
 +
<ul>
 +
<li>3 iterations designed in 3Duf and tested</li>
 +
</ul>
 +
</li>
 +
<li>Alternative Tree Primitive Designs
 +
<ul>
 +
<li>
 +
Given repeat difficulties faced while using trees in designs, alternative designs were tested to determine if there was a better design
 +
</li>
 +
<li>3 iterations designed in 3Duf and tested</li>
 +
</ul>
 +
</li>
 +
<li>Transformation Chip
 +
<ul>
 +
<li>6 Iterations designed in Fluigi</li>
 +
<li>2 Iterations designed in 3Duf and tested</li>
 +
</ul>
 +
</li>
 +
<li>Cell Sorting
 +
<ul>
 +
<li>1 iteration milled and tested </li>
 +
<li>Magnetic particles run through chip</li>
 +
</ul>
 +
</li>
 +
<li>Attended Northeastern iGEM practice conference (NEGEM) at BU
 +
<ul>
 +
<li>Received feedback on iGEM narrative</li>
 +
<li>Developed project ideas based off of feedback</li>
 +
</ul>
 +
</li>
 +
<li>MARS Repository begins to form after talking with researchers from CILSE’s Biological Design Center </li>
 +
</ul>
 +
</div>
 +
</div>
 +
</div>
  
−
<div class="column half_size">
+
<div class="main main-raised" style="margin-top:5%" id ="Chip_Repo">
−
<h5>What should this page have?</h5>
+
<div class="container">
−
<ul>
+
<h1>July</h1>
−
<li>Chronological notes of what your team is doing.</li>
+
<div class="text" style="margin-bottom:3%;">
−
<li> Brief descriptions of daily important events.</li>
+
<ul>
−
<li>Pictures of your progress. </li>
+
<li>Alternative Tree Primitive Designs
−
<li>Mention who participated in what task.</li>
+
<ul>
−
</ul>
+
<li>1 iteration designed in 3Duf and tested</li>
 +
<li>1This design was markedly better at dispensing liquid equally, therefore it was used in later designs</li>
 +
</ul>
 +
</li>
 +
<li>Cell Lysis Chip
 +
<ul>
 +
<li>3 iterations designed in 3Duf and tested</li>
 +
<li>Changes made to fix issues with fluid input and mixing </li>
 +
<li>Initial draft of protocol written</li>
 +
</ul>
 +
</li>
 +
<li>Wetlab Collaboration Chip
 +
<ul>
 +
<li>4 iterations milled</li>
 +
<li>Sealing issues improved upon</li>
 +
<li>Testing done with shared liquid input</li>
 +
</ul>
 +
</li>
 +
<li>DNA Digestion Chip
 +
<ul>
 +
<li>4 iterations milled </li>
 +
<li>Iterations made to better replicate protocol on a chip </li>
 +
<li>Pipetting chamber designed and optimized for chip</li>
 +
</ul>
 +
</li>
 +
<li>BostonU Hardware visits BosLab during monthly showcase
 +
<ul>
 +
<li>Provided insight into what small scale biohacker space is like, and what synthetic biology community is looking for in microfluidics</li>
 +
</ul>
 +
</li>
 +
<li>Fluid Functionality begins to be developed
 +
<ul>
 +
<li>Idea of primitive level analysis developed along with some qualitative failures</li>
 +
</ul>
 +
</li>
 +
<li>Worked with Neptune (2016 BostonU Hardware Team) automated syringe pumps tried to increase accuracy and improve function</li>
 +
<li>MARS Repository chips began to be placed in subsections of isolation, modification, and quantification</li>
 +
<li>Transformation Chip
 +
<ul>
 +
<li>2 iterations designed in Fluigi</li>
 +
<li>8 iterations designed in 3Duf and tested</li>
 +
<li>Changes made to overall design as well as valve sizes</li>
 +
</ul>
 +
</li>
 +
<li>Summer Pathways
 +
<ul>
 +
<li>Participated in Summer Pathways alongside the BostonU Wetlab Team</li>
 +
<li>Created an interactive microfluidics design activity for the attending high school students</li>
 +
<li>Engaged with students to design microfluidic devices based on synbio protocols </li>
 +
</ul>
 +
</li>
 +
<li>Harvard iGEM Team Collaboration
 +
<ul>
 +
<li>Initial meetings to discuss plans </li>
 +
</ul>
 +
</li>
 +
<li>WPI iGEM Team Collaboration
 +
<ul>
 +
<li>During the initial Skype call we discussed the nature of their lead assay and how it might be moved onto a microfluidic</li>
 +
<li>Their team visited our lab and performed verified their assay’s functionality on the BU spectrometer</li>
 +
</ul>
 +
</li>
 +
<li>Tutorial Videos
 +
<ul>
 +
<li>PDMS video filmed</li>
 +
</ul>
 +
</li>
 +
<li>Peristaltic Pump Chip<sup>[4]</sup>
 +
<ul>
 +
<li>Design inspired by another paper with different geometries</li>
 +
<li>4 iterations designed in 3Duf and tested</li>
 +
</ul>
 +
</li>
 +
<li>Metering Primitive
 +
<ul>
 +
<li>In order to allow for accurate volume dispensing on a microfluidic device, a metering primitive inspired by the peristaltic pump was designed</li>
 +
<li>2 iterations designed in 3Duf and tested</li>
 +
</ul>
 +
</li>
 +
</ul>
 +
</div>
 +
</div>
  
−
</div>
+
</div>
 +
 
 +
<div class="main main-raised" style="margin-top:5%" id="FF">
 +
<div class="container">
 +
<h1>August</h1>
 +
<div class="text" style="margin-bottom:3%;">
 +
<ul>
 +
<li>Transformation Chip
 +
<ul>
 +
<li>13 iterations designed in 3Duf and tested</li>
 +
<li>Different valve dimensions tested</li>
 +
</ul>
 +
</li>
 +
<li>Ligation Chip
 +
<ul>
 +
<li>2 iterations designed in 3Duf</li>
 +
</ul>
 +
</li>
 +
<li>Tutorial Videos
 +
<ul>
 +
<li>PDMS Video finalized</li>
 +
<li>Milling Video recording and scripting completed</li>
 +
</ul>
 +
</li>
 +
<li>DNA Digestion
 +
<ul>
 +
<li>2 iterations milled and tested</li>
 +
</ul>
 +
</li>
 +
<li>Wetlab Collaboration Chip
 +
<ul>
 +
<li>1 iteration milled and tested</li>
 +
</ul>
 +
</li>
 +
<li>Cell Lysis Chip
 +
<ul>
 +
<li>Design finalized </li>
 +
<li>Testing with magnetic particles in chamber</li>
 +
</ul>
 +
</li>
 +
<li>Cell Culturing<sup>[6]</sup>
 +
<ul>
 +
<li>Initial CAD model designed and tested</li>
 +
<li>Spin coated PDMS </li>
 +
<li>Second and final CAD model designed and tested </li>
 +
</ul>
 +
</li>
 +
<li>Wiki
 +
<ul>
 +
<li>Initial pages started to be constructed </li>
 +
<li>Wiki architecture organized</li>
 +
</ul>
 +
</li>
 +
<li>Fluid Functionality
 +
<ul>
 +
<li>Quantitative tests begin to be developed</li>
 +
</ul>
 +
</li>
 +
 
 +
</ul>
 +
</div>
 +
</div>
 +
 
 +
</div>
 +
 
 +
<div class="main main-raised" style="margin-top:5%;" id="uF_101">
 +
<div class="container">
 +
<h1>September</h1>
 +
<div class="text" style="margin-bottom:3%;">
 +
<ul>
 +
<li>Transformation Chip
 +
<ul>
 +
<li>1 iteration designed in 3Duf and tested</li>
 +
<li>Different valve dimension tested</li>
 +
</ul>
 +
</li>
 +
<li>Tutorial Videos
 +
<ul>
 +
<li>Milling video finalized</li>
 +
<li>Assembly video filmed and scripted</li>
 +
<li>Cleaning video filmed and audio recorded</li>
 +
</ul>
 +
</li>
 +
<li>Fluid Functionality
 +
<ul>
 +
<li>Channels quantitative tests finalized</li>
 +
</ul>
 +
</li>
 +
<li>Antibiotic Resistance<sup>[5]</sup>
 +
<ul>
 +
<li>Initial design developed</li>
 +
</ul>
 +
</li>
 +
<li>Wetlab Collaboration Chip
 +
<ul>
 +
<li>2 Iterations milled and tested </li>
 +
<li>Protocol and design finalized </li>
 +
<li>Chip tested by Wetlab </li>
 +
</ul>
 +
</li>
 +
</ul>
 +
</div>
 +
</div>
 +
 
 +
</div>
 +
 
 +
<div class="main main-raised" id="uF_101" style="margin-top:5%;">
 +
<div class="container">
 +
<h1>October</h1>
 +
<div class="text" style="margin-bottom:3%;">
 +
<ul>
 +
<li>Transformation Chip
 +
<ul>
 +
<li>4 iterations designed in 3Duf and tested</li>
 +
<li>Different metering scales tested</li>
 +
</ul>
 +
</li>
 +
<li>PCR Chip
 +
<ul>
 +
<li>4 iterations designed in 3Duf and tested</li>
 +
</ul>
 +
</li>
 +
<li>Cell Sorting
 +
<ul>
 +
<li>2 iterations milled and tested </li>
 +
<li>Protocol finalized and documented</li>
 +
</ul>
 +
</li>
 +
<li>Ligation Chip
 +
<ul>
 +
<li>1 iteration designed in 3Duf</li>
 +
</ul>
 +
</li>
 +
<li>Video tutorials
 +
<ul>
 +
<li>Assembly video finalized</li>
 +
<li>Cleaning video finalized</li>
 +
</ul>
 +
</li>
 +
<li>WPI iGEM Team Collaboration chip design documented finalized</li>
 +
<li>Attended Northeastern iGEM practice conference #2 (NEGEM) at MIT
 +
<ul>
 +
<li>Received feedback on iGEM presentation</li>
 +
<li>Incorporated feedback for final presentation</li>
 +
</ul>
 +
</li>
 +
<li>Fluid Functionality
 +
<ul>
 +
<li>Valve quantitative test finalized </li>
 +
<li>Mixer quantitative test finalized </li>
 +
</ul>
 +
</li>
 +
<li>Antibiotic Resistance
 +
<ul>
 +
<li>Design finalized using 3Duf</li>
 +
<li>Milled and documented</li>
 +
</ul>
 +
</li>
 +
 
 +
<li>Harvard iGEM Collaboration
 +
<ul>
 +
<li>Validated Harvard optical density sensor</li>
 +
</ul>
 +
</li>
 +
 
 +
</ul>
 +
</div>
 +
</div>
 +
 
 +
</div>
 +
<div class="main main-raised" style="margin-top:5%;" id="uF_101">
 +
<div class="container">
 +
<div class="text" style="margin-bottom:3%;">
 +
<h1>Citations</h1>
 +
<ol>
 +
<li>Tourlousse, D. M., Ahmad, F., Stedtfeld, R. D., Seyrig, G., Tiedje, J. M., & Hashsham, S. A. (2012). A polymer microfluidic chip for quantitative detection of multiple water- and foodborne pathogens using real-time fluorogenic loop-mediated isothermal amplification. Biomedical Microdevices, 14(4), 769–778. <a href = "https://doi.org/10.1007/s10544-012-9658-3">https://doi.org/10.1007/s10544-012-9658-3</a>
 +
</li>
 +
<li>Mohan, R., Mukherjee, A., Sevgen, S. E., Sanpitakseree, C., Lee, J., Schroeder, C. M., & Kenis, P. J. A. (2013). A multiplexed microfluidic platform for rapid antibiotic susceptibility testing. Biosensors and Bioelectronics, 49, 118–125. <a href = "https://doi.org/10.1016/j.bios.2013.04.046">https://doi.org/10.1016/j.bios.2013.04.046</a>
 +
</li>
 +
<li>Liang-Hsuan Lu, Kee Suk Ryu, & Chang Liu. (2002). A magnetic microstirrer and array for microfluidic mixing. Journal of Microelectromechanical Systems, 11(5), 462–469. <a href = "https://doi.org/10.1109/jmems.2002.802899">https://doi.org/10.1109/jmems.2002.802899</a>
 +
 
 +
</li><li>Nguyen, T. V., Duncan, P. N., Ahrar, S., & Hui, E. E. (2012). Semi-autonomous liquid handling via on-chip pneumatic digital logic. Lab on a Chip, 12(20), 3991. <a href = "https://doi.org/10.1039/c2lc40466d">https://doi.org/10.1039/c2lc40466d</a></li>
 +
<li>Hou, H. W., Bhagat, A. A. S., Lin Chong, A. G., Mao, P., Wei Tan, K. S., Han, J., & Lim, C. T. (2010). Deformability based cell margination—A simple microfluidic design for malaria-infected erythrocyte separation. Lab on a Chip, 10(19), 2605. <a href = "https://doi.org/10.1039/c003873c">https://doi.org/10.1039/c003873c</a></li>
 +
                                        <li>Lee KS, Boccazzi P, Sinskey AJ, Ram RJ. Microfluidic chemostat and turbidostat with flow rate, oxygen, and temperature control for dynamic continuous culture. Lab Chip. 2011;11(10):1730-9.<a href = "https://doi.org/10.1039/c1lc20019d">https://doi.org/10.1039/c1lc20019d</a>
 +
</li>
 +
</ol>
 +
</div>
 +
</div>
 +
</div>
 +
 
 +
  </div>
 +
 
 +
<!-- THIS IS FOOTER -->
 +
<div class="wrapper" style="background:#1c1f1f; margin-top:0px;margin-right:0px !important; margin-left:0px !important;" id="Footer">
 +
  <div class="container" style="text-align:center !important">
 +
 
 +
    <div class="col-md-2" style="color:white; margin-bottom:30px; margin-top:5px;">
 +
      <h3>CONTACT US</h3>
 +
    <div style="text-align:center;">
 +
      <a href="mailto:igembuhw@gmail.com">
 +
      <img src="https://static.igem.org/mediawiki/2017/7/74/MARS_WHITEEmail.png" style="height:60px; margin-top:20px;">
 +
      </a>
 +
        <a href="https://www.instagram.com/buigemhardware/?hl=en">
 +
        <img src="https://static.igem.org/mediawiki/2017/9/93/MARS_Final_insta.png" style="height:60px; margin-top:20px;">
 +
      </a>
 +
          <a href="https://twitter.com/igemhwbu">
 +
          <img src="https://static.igem.org/mediawiki/2017/b/b6/MARS_Twitter_White.png" style="height:60px; margin-top:20px;">
 +
          </a>
 +
      </div>
 +
      </div>
 +
      <div class="col-md-10" style="margin-bottom:30px;">
 +
        <img src="https://static.igem.org/mediawiki/2017/0/0e/MARS_SponsorsFinal.png" style="width:100%; margin-top:30px;" usemap="#image-map">
 +
    </div>
 +
</div>
 +
 
 +
</div>
  
−
<div class="column half_size">
 
−
<h5>Inspiration</h5>
 
−
<p>You can see what others teams have done to organize their notes:</p>
 
  
−
<ul>
 
−
<li><a href="https://2014.igem.org/Team:ATOMS-Turkiye/Notebook">2014 ATOMS-Turkiye</a></li>
 
−
<li><a href="https://2014.igem.org/Team:Tec-Monterrey/ITESM14_project.html#tab_notebook">2014 Tec Monterrey</a></li>
 
−
<li><a href="https://2014.igem.org/Team:Kyoto/Notebook/Magnetosome_Formation#title">2014 Kyoto</a></li>
 
−
<li><a href="https://2014.igem.org/Team:Cornell/notebook">2014 Cornell</a></li>
 
−
</ul>
 
  
 
</div>
 
</div>
 +
</body>
 +
 
</html>
 
</html>
 +
 +
{{Team:BostonU_Hardware/Javascript}}

Latest revision as of 02:22, 1 November 2017

BostonU_HW

Attributions
Notebook

May

  • The BostonU Wetlab and Hardware iGEM teams participated in joint Wetlab safety and protocol training
  • The team performed an initial literature review in order to learn more about the types of microfluidic devices being design
  • The team was given a software overview/explanation of the three CIDAR lab microfluidic design tools: 3Duf, Fluigi, and Neptune
  • The team was given an overview/explanation of the CIDAR lab microfluidic design manufacturing process: Makerfluidics

June

  • From the literature review, each member chose one chip of interest to replicate and test
    1. LAMP Chip[1]
      • 3 iterations designed in Fluigi
      • 5 iterations designed in 3Duf and tested
    2. Multiplex Drug Testing Chip[2]
      • 2 iterations designed in 3Duf and tested
      • 1 iteration designed in Fluigi
    3. Magnetic Mixer Chip[3]
      • 5 Iterations milled and tested
      • Magnetic particles introduced into chip
      • Experimented with different protocols
  • BostonU Wetlab iGEM Team Collaboration began
    • At the initial meeting BostonU Wetlab provided BostonU Hardware with a protocol they thought could be performed in a microfluidic device
    • 6 potential chips designed for Wetlab collaboration
  • Submitted Microfluidics iGEM Poll to the iGEM collaborations page
  • Volume Dispensing Chips
    • 3 iterations designed in 3Duf and tested
  • Alternative Tree Primitive Designs
    • Given repeat difficulties faced while using trees in designs, alternative designs were tested to determine if there was a better design
    • 3 iterations designed in 3Duf and tested
  • Transformation Chip
    • 6 Iterations designed in Fluigi
    • 2 Iterations designed in 3Duf and tested
  • Cell Sorting
    • 1 iteration milled and tested
    • Magnetic particles run through chip
  • Attended Northeastern iGEM practice conference (NEGEM) at BU
    • Received feedback on iGEM narrative
    • Developed project ideas based off of feedback
  • MARS Repository begins to form after talking with researchers from CILSE’s Biological Design Center

July

  • Alternative Tree Primitive Designs
    • 1 iteration designed in 3Duf and tested
    • 1This design was markedly better at dispensing liquid equally, therefore it was used in later designs
  • Cell Lysis Chip
    • 3 iterations designed in 3Duf and tested
    • Changes made to fix issues with fluid input and mixing
    • Initial draft of protocol written
  • Wetlab Collaboration Chip
    • 4 iterations milled
    • Sealing issues improved upon
    • Testing done with shared liquid input
  • DNA Digestion Chip
    • 4 iterations milled
    • Iterations made to better replicate protocol on a chip
    • Pipetting chamber designed and optimized for chip
  • BostonU Hardware visits BosLab during monthly showcase
    • Provided insight into what small scale biohacker space is like, and what synthetic biology community is looking for in microfluidics
  • Fluid Functionality begins to be developed
    • Idea of primitive level analysis developed along with some qualitative failures
  • Worked with Neptune (2016 BostonU Hardware Team) automated syringe pumps tried to increase accuracy and improve function
  • MARS Repository chips began to be placed in subsections of isolation, modification, and quantification
  • Transformation Chip
    • 2 iterations designed in Fluigi
    • 8 iterations designed in 3Duf and tested
    • Changes made to overall design as well as valve sizes
  • Summer Pathways
    • Participated in Summer Pathways alongside the BostonU Wetlab Team
    • Created an interactive microfluidics design activity for the attending high school students
    • Engaged with students to design microfluidic devices based on synbio protocols
  • Harvard iGEM Team Collaboration
    • Initial meetings to discuss plans
  • WPI iGEM Team Collaboration
    • During the initial Skype call we discussed the nature of their lead assay and how it might be moved onto a microfluidic
    • Their team visited our lab and performed verified their assay’s functionality on the BU spectrometer
  • Tutorial Videos
    • PDMS video filmed
  • Peristaltic Pump Chip[4]
    • Design inspired by another paper with different geometries
    • 4 iterations designed in 3Duf and tested
  • Metering Primitive
    • In order to allow for accurate volume dispensing on a microfluidic device, a metering primitive inspired by the peristaltic pump was designed
    • 2 iterations designed in 3Duf and tested

August

  • Transformation Chip
    • 13 iterations designed in 3Duf and tested
    • Different valve dimensions tested
  • Ligation Chip
    • 2 iterations designed in 3Duf
  • Tutorial Videos
    • PDMS Video finalized
    • Milling Video recording and scripting completed
  • DNA Digestion
    • 2 iterations milled and tested
  • Wetlab Collaboration Chip
    • 1 iteration milled and tested
  • Cell Lysis Chip
    • Design finalized
    • Testing with magnetic particles in chamber
  • Cell Culturing[6]
    • Initial CAD model designed and tested
    • Spin coated PDMS
    • Second and final CAD model designed and tested
  • Wiki
    • Initial pages started to be constructed
    • Wiki architecture organized
  • Fluid Functionality
    • Quantitative tests begin to be developed

September

  • Transformation Chip
    • 1 iteration designed in 3Duf and tested
    • Different valve dimension tested
  • Tutorial Videos
    • Milling video finalized
    • Assembly video filmed and scripted
    • Cleaning video filmed and audio recorded
  • Fluid Functionality
    • Channels quantitative tests finalized
  • Antibiotic Resistance[5]
    • Initial design developed
  • Wetlab Collaboration Chip
    • 2 Iterations milled and tested
    • Protocol and design finalized
    • Chip tested by Wetlab

October

  • Transformation Chip
    • 4 iterations designed in 3Duf and tested
    • Different metering scales tested
  • PCR Chip
    • 4 iterations designed in 3Duf and tested
  • Cell Sorting
    • 2 iterations milled and tested
    • Protocol finalized and documented
  • Ligation Chip
    • 1 iteration designed in 3Duf
  • Video tutorials
    • Assembly video finalized
    • Cleaning video finalized
  • WPI iGEM Team Collaboration chip design documented finalized
  • Attended Northeastern iGEM practice conference #2 (NEGEM) at MIT
    • Received feedback on iGEM presentation
    • Incorporated feedback for final presentation
  • Fluid Functionality
    • Valve quantitative test finalized
    • Mixer quantitative test finalized
  • Antibiotic Resistance
    • Design finalized using 3Duf
    • Milled and documented
  • Harvard iGEM Collaboration
    • Validated Harvard optical density sensor

Citations

  1. Tourlousse, D. M., Ahmad, F., Stedtfeld, R. D., Seyrig, G., Tiedje, J. M., & Hashsham, S. A. (2012). A polymer microfluidic chip for quantitative detection of multiple water- and foodborne pathogens using real-time fluorogenic loop-mediated isothermal amplification. Biomedical Microdevices, 14(4), 769–778. https://doi.org/10.1007/s10544-012-9658-3
  2. Mohan, R., Mukherjee, A., Sevgen, S. E., Sanpitakseree, C., Lee, J., Schroeder, C. M., & Kenis, P. J. A. (2013). A multiplexed microfluidic platform for rapid antibiotic susceptibility testing. Biosensors and Bioelectronics, 49, 118–125. https://doi.org/10.1016/j.bios.2013.04.046
  3. Liang-Hsuan Lu, Kee Suk Ryu, & Chang Liu. (2002). A magnetic microstirrer and array for microfluidic mixing. Journal of Microelectromechanical Systems, 11(5), 462–469. https://doi.org/10.1109/jmems.2002.802899
  4. Nguyen, T. V., Duncan, P. N., Ahrar, S., & Hui, E. E. (2012). Semi-autonomous liquid handling via on-chip pneumatic digital logic. Lab on a Chip, 12(20), 3991. https://doi.org/10.1039/c2lc40466d
  5. Hou, H. W., Bhagat, A. A. S., Lin Chong, A. G., Mao, P., Wei Tan, K. S., Han, J., & Lim, C. T. (2010). Deformability based cell margination—A simple microfluidic design for malaria-infected erythrocyte separation. Lab on a Chip, 10(19), 2605. https://doi.org/10.1039/c003873c
  6. Lee KS, Boccazzi P, Sinskey AJ, Ram RJ. Microfluidic chemostat and turbidostat with flow rate, oxygen, and temperature control for dynamic continuous culture. Lab Chip. 2011;11(10):1730-9.https://doi.org/10.1039/c1lc20019d